>AFO130 (AFO130Q) /CSM/AF/AFO1-B/AFO130Q.Seq.d/
XXXXXXXXXXTNATTTTNATNNTCATAGTAGTGGTGTTTNCTTTTATTATGATAATAATT
CNCNAGCTTATTATTNNTATGATTCAACAACAAATTCCTATTTACCATTCATACCAAAAT
CAAATGATAAGGCTATTGATCAAAGTGGTACTGATGCAATCAATACAACAATGACATCTT
CAACAACAACCACAACCACAACAGCAACAACAACCACAACAGCAACAACATCATCACCAT
CAATAGCAACTTCAACATCNNCCACTTTAATGNCAAAAACTGANACACCAACAAATGATC
AAACAAAACAAGAAGATAAACGTATAGTTGTATCACATGTGNCAAAATTCAATTCATTTT
TA

---XFXXHSSGVXFYYDNNSXAYYXYDSTTNSYLPFIPKSNDKAIDQSGTDAINTTMTSS
TTTTTTTATTTTTATTSSPSIATSTSXTLMXKTXTPTNDQTKQEDKRIVVSHVXKFNSFL

Frame A:
---XFXXHSSGVXFYYDNNSXAYYXYDSTTNSYLPFIPKSNDKAIDQSGTDAINTTMTSS
TTTTTTTATTTTTATTSSPSIATSTSXTLMXKTXTPTNDQTKQEDKRIVVSHVXKFNSFL


Frame B:
---ixxxivvvfxfimiiixxliixmiqqqipiyhsyqnqmirllikvvlmqsiqq*hlq
qqpqpqqqqqpqqqqhhhhq*qlqhxpl*xqklxhqqmikqnkkinv*lyhmxqnsihf


Frame C:
---fxxs**wcxlll***fxsllxx*fnnkflftihtkik**gy*skwy*cnqynndifn
nnhnhnsnnnhnsnniitinsnfnixhfnxkn*xtnk*snktrr*tyscitcxkiqfif

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CFF643 (CFF643Q) /CSM/CF/CFF6-B/CFF643Q.Seq.d/                        246   2e-64
AFO130 (AFO130Q) /CSM/AF/AFO1-B/AFO130Q.Seq.d/                        246   2e-64
AFD672 (AFD672Q) /CSM/AF/AFD6-C/AFD672Q.Seq.d/                        208   4e-53
VHO696 (VHO696Q) /CSM/VH/VHO6-D/VHO696Q.Seq.d/                         36   0.35
VHN517 (VHN517Q) /CSM/VH/VHN5-A/VHN517Q.Seq.d/                         36   0.35
VHI226 (VHI226Q) /CSM/VH/VHI2-B/VHI226Q.Seq.d/                         36   0.35
VHH501 (VHH501Q) /CSM/VH/VHH5-A/VHH501Q.Seq.d/                         36   0.35
VHE680 (VHE680Q) /CSM/VH/VHE6-D/VHE680Q.Seq.d/                         36   0.35
VHD785 (VHD785Q) /CSM/VH/VHD7-D/VHD785Q.Seq.d/                         36   0.35
VFK437 (VFK437Q) /CSM/VF/VFK4-B/VFK437Q.Seq.d/                         36   0.35

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC131855|AC131855.3 Rattus norvegicus clone CH230-200K6, WORKING DRAFT SEQUENCE, 1 ordered piece.    42  4.6  1
AC103314|AC103314.6 Rattus norvegicus clone CH230-160G3, WORKING DRAFT SEQUENCE, 2 unordered pieces.    42  4.6  1
AC120079|AC120079.5 Rattus norvegicus clone CH230-169C15, WORKING DRAFT SEQUENCE, 3 unordered pieces.    42  4.6  1
AC099279|AC099279.9 Rattus norvegicus clone CH230-29D7, WORKING DRAFT SEQUENCE, 11 unordered pieces.    42  4.6  1
AC096091|AC096091.6 Rattus norvegicus clone CH230-14N8, WORKING DRAFT SEQUENCE, 16 unordered pieces.    42  4.6  1
BF243956|BF243956.1 601876620F1 NIH_MGC_55 Homo sapiens cDNA clone IMAGE:4105013 5', mRNA sequence.    42  4.6  1
AL929185|AL929185.9 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-193L2.    42  4.6  1
AC136636|AC136636.1 Mus musculus chromosome UNK clone RP24-183E22, WORKING DRAFT SEQUENCE, 57 unordered pieces.    42  4.6  1
AZ970658|AZ970658.1 2M0243D22R Mouse 10kb plasmid UUGC2M library Mus musculus genomic clone UUGC2M0243D22 R, DNA sequence.    42  4.6  1
AX417038|AX417038.1 Sequence 4029 from Patent WO0228891.          42  4.6  1

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3039690
Number of Hits to DB: 208,907,813

 psg: 0.83  gvh: 0.35  alm: 0.56  top: 0.53  tms: 0.00  mit: 0.17  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 36.0 %: cytoplasmic
	 16.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for AFO130 is nuc

>AFO130Z.Seq
----------TNATTTTNATNNTCATAGTAGTGGTGTTTNCTTTTATTATGATAATAATT
CNCNAGCTTATTATTNNTATGATTCAACAACAAATTCCTATTTACCATTCATACCAAAAT
CAAATGATAAGGCTATTGATCAAAGTGGTACTGATGCAATCAATACAACAATGACATCTT
CAACAACAACCACAACCACAACAGCAACAACAACCACAACAGCAACAACATCATCACCAT
CAATAGCAACTTCAACATCNNCCACTTTAATGNCAAAAACTGANACACCAACAAATGATC
AAACAAAACAAGAAGATAAACGTATAGTTGTATCACATGTGNCAAAATTCAATTCATTTT
TA