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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHH141
Library VH
(Link to library)
Clone ID VHH141
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12301-1
Original site URL
Representative seq. ID VHH141P
(Link to Original site)
Representative DNA sequence 
>VHH141 (VHH141Q) /CSM/VH/VHH1-B/VHH141Q.Seq.d/
ATAAAAAACTTTTTATAAATAATATATACATACAATGGGTAACAGAGCATTCAAAGCACA
CAACGGTCACTACTTAAGCGCTGAACACGATCACGTCAAGACCCACCACGGTCATCACGA
TCATCACACTCACTTCCATATCGAAAACCACGGAAGCAAAGTTGCCTTAAGAACCCATTG
CGGTAAATACGTTTCAATTGGTGACCATAAACAAGTTTACCTCTCACACCACTTACACGG
TGACCACTCACTCTTCCACTTAGAACACCACCACGGTAAAGTCTCAATCAAAGGTCATCA
CCACCACTACATCTCTGTTGACGGTCACGGTCATGTCTCTACCTCCCATCACCACGATCA
TCATGCTACTTTCGAAGAACACATCTTATAAACAAATAAATAAAACAATAAAAATGTTGT
AAAATATTAAAAAATAAAAAAAAAAXXXXXXXXXXATAAAAAACTTTTTATAAATAATAT
ATACATACAATGGGTAACAGAGCATTCAAAGCACACAACGGTCACTANTTAAGCGNTGAA
CACGATCACGTCAAGACCCACCACGGTCATCACGATCATCACACTCACTTCCATATCGAA
AACCACGGAAGCAAAGTTGCCTTAAGAACCCATTGCGGTAAATACGTTTCAATTGGTGAC
CATAAACAAGTTTACCTCTCACACCACTTACACGGTGACCACTCACTCTTCCANTTAGAA
CACCACCACGGTAAAGTNTCAATCAAAGGTCATCACCACCACTACATCTCTGTTGACGGT
CACGGTCATGTCTCNACCTCCCATCACCACGATCATCATGCTACTTTCGAAGAACACATC
TNANAAACAAATAAATAAAACAATAAAAATGTGTAAAATATTAAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
iknfl*iiytyng*qsiqstqrsllkr*trsrqdpprssrsshslpyrkprkqsclknpl
r*irfnw*p*tslpltpltr*pltlplrtppr*slnqrsspplhlc*rsrsclylpsprs
scyfrrthlink*ikq*KCCKILKNKKK---

---KKLFINNIYIQWVTEHSKHTTVTX*axntitsrpttvitiitltsisktteaklp*e
piavntfqlvtinkftshttytvtthssx*ntttvkxqskvittttslltvtvmsxppit
tiimllskntsxkqinktikmckilk
Translated Amino Acid sequence (All Frames) 
Frame A:
iknfl*iiytyng*qsiqstqrsllkr*trsrqdpprssrsshslpyrkprkqsclknpl
r*irfnw*p*tslpltpltr*pltlplrtppr*slnqrsspplhlc*rsrsclylpsprs
scyfrrthlink*ikq*KCCKILKNKKK---

---iknfl*iiytyng*qsiqstqrslxkr*trsrqdpprssrsshslpyrkprkqsclk
nplr*irfnw*p*tslpltpltr*pltlpxrtppr*sxnqrsspplhlc*rsrsclxlps
prsscyfrrthlxnk*ikq*kcvky*k

Frame B:
*ktfyk*yihtmgnrafkahnghylsaehdhvkthhghhdhhthfhienhgskvalrthc
gkyvsigdhkqvylshhlhgdhslfhlehhhgkvsikghhhhyisvdghghvstshhhdh
hatfeehil*tnk*nnknvvky*kikkk---

---*ktfyk*yihtmgnrafkahnghxlsxehdhvkthhghhdhhthfhienhgskvalr
thcgkyvsigdhkqvylshhlhgdhslfxlehhhgkvsikghhhhyisvdghghvstshh
hdhhatfeehixxtnk*nnknv*nik

Frame C:
kklfinniyiqwvtehskhttvtt*alntitsrpttvitiitltsisktteaklp*epia
vntfqlvtinkftshttytvtthsst*ntttvksqskvittttslltvtvmslppittii
mllskntsykqinktikml*nikk*kk---

---KKLFINNIYIQWVTEHSKHTTVTX*axntitsrpttvitiitltsisktteaklp*e
piavntfqlvtinkftshttytvtthssx*ntttvkxqskvittttslltvtvmsxppit
tiimllskntsxkqinktikmckilk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHK281 (VHK281Q) /CSM/VH/VHK2-D/VHK281Q.Seq.d/                        767   0.0
VHH141 (VHH141Q) /CSM/VH/VHH1-B/VHH141Q.Seq.d/                        767   0.0
VFI246 (VFI246Q) /CSM/VF/VFI2-B/VFI246Q.Seq.d/                        767   0.0
VFE103 (VFE103Q) /CSM/VF/VFE1-A/VFE103Q.Seq.d/                        767   0.0
VHE632 (VHE632Q) /CSM/VH/VHE6-B/VHE632Q.Seq.d/                        759   0.0
VSA785 (VSA785Q) /CSM/VS/VSA7-D/VSA785Q.Seq.d/                        664   0.0
SSG165 (SSG165Q) /CSM/SS/SSG1-C/SSG165Q.Seq.d/                        660   0.0
SSB534 (SSB534Q) /CSM/SS/SSB5-B/SSB534Q.Seq.d/                        642   0.0
VFN807 (VFN807Q) /CSM/VF/VFN8-A/VFN807Q.Seq.d/                        527   e-149
VHL371 (VHL371Q) /CSM/VH/VHL3-C/VHL371Q.Seq.d/                        311   1e-83
own update 2002.12.29
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ432032) Dictyostelium discoideum cDNA clone:ddv17k12, 3' ...   767   0.0    1
(BJ424209) Dictyostelium discoideum cDNA clone:ddv51a22, 5' ...   767   0.0    1
(BJ422216) Dictyostelium discoideum cDNA clone:ddv45a11, 5' ...   767   0.0    1
(BJ413917) Dictyostelium discoideum cDNA clone:ddv17k12, 5' ...   767   0.0    1
(BJ412561) Dictyostelium discoideum cDNA clone:ddv9e01, 5' e...   767   0.0    1
(BJ420789) Dictyostelium discoideum cDNA clone:ddv40o08, 5' ...   759   0.0    1
(BJ440957) Dictyostelium discoideum cDNA clone:ddv45a11, 3' ...   735   0.0    1
(U13671) Dictyostelium discoideum hisactophilin II (hsII) mR...   700   0.0    1
(BJ439432) Dictyostelium discoideum cDNA clone:ddv40o08, 3' ...   688   0.0    1
(AU261737) Dictyostelium discoideum vegetative cDNA clone:VS...   664   0.0    1
dna update 2008.11. 6
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P42526) RecName: Full=Hisactophilin-2; AltName: Full=Histidine-...   154   5e-36
(P13231) RecName: Full=Hisactophilin-1; AltName: Full=Histidine-...   141   3e-32
AF544403_1(AF544403|pid:none) Expression vector pNCO113-hisactop...   141   3e-32
EF493211_1(EF493211|pid:none) Expression vector pNCO-HISACT-(C49...   137   4e-31
FN322026_1(FN322026|pid:none) Schistosoma japonicum isolate Anhu...    38   0.52
FN322023_1(FN322023|pid:none) Schistosoma japonicum isolate Anhu...    38   0.52
FN322029_1(FN322029|pid:none) Schistosoma japonicum isolate Anhu...    37   0.68
FN322027_1(FN322027|pid:none) Schistosoma japonicum isolate Anhu...    37   0.68
FN322028_1(FN322028|pid:none) Schistosoma japonicum isolate Anhu...    37   0.68
CP000482_2739(CP000482|pid:none) Pelobacter propionicus DSM 2379...    37   0.89
protein update 2009. 7.12
PSORT 

 psg: 0.60  gvh: 0.24  alm: 0.64  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.20  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: nuclear
	 16.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: plasma membrane

>> prediction for VHH141 is nuc
5' end seq. ID VHH141F
5' end seq. 
>VHH141F.Seq
ATAAAAAACTTTTTATAAATAATATATACATACAATGGGTAACAGAGCATTCAAAGCACA
CAACGGTCACTACTTAAGCGCTGAACACGATCACGTCAAGACCCACCACGGTCATCACGA
TCATCACACTCACTTCCATATCGAAAACCACGGAAGCAAAGTTGCCTTAAGAACCCATTG
CGGTAAATACGTTTCAATTGGTGACCATAAACAAGTTTACCTCTCACACCACTTACACGG
TGACCACTCACTCTTCCACTTAGAACACCACCACGGTAAAGTCTCAATCAAAGGTCATCA
CCACCACTACATCTCTGTTGACGGTCACGGTCATGTCTCTACCTCCCATCACCACGATCA
TCATGCTACTTTCGAAGAACACATCTTATAAACAAATAAATAAAACAATAAAAATGTTGT
AAAATATTAAAAAATAAAAAAAAAANNNNNNNNNN
Length of 5' end seq. 455
3' end seq. ID VHH141Z
3' end seq. 
>VHH141Z.Seq
NNNNNNNNNNATAAAAAACTTTTTATAAATAATATATACATACAATGGGTAACAGAGCAT
TCAAAGCACACAACGGTCACTANTTAAGCGNTGAACACGATCACGTCAAGACCCACCACG
GTCATCACGATCATCACACTCACTTCCATATCGAAAACCACGGAAGCAAAGTTGCCTTAA
GAACCCATTGCGGTAAATACGTTTCAATTGGTGACCATAAACAAGTTTACCTCTCACACC
ACTTACACGGTGACCACTCACTCTTCCANTTAGAACACCACCACGGTAAAGTNTCAATCA
AAGGTCATCACCACCACTACATCTCTGTTGACGGTCACGGTCATGTCTCNACCTCCCATC
ACCACGATCATCATGCTACTTTCGAAGAACACATCTNANAAACAAATAAATAAAACAATA
AAAATGTGTAAAATATTAAAAA
Length of 3' end seq. 442
Connected seq. ID VHH141P
Connected seq. 
>VHH141P.Seq
ATAAAAAACTTTTTATAAATAATATATACATACAATGGGTAACAGAGCATTCAAAGCACA
CAACGGTCACTACTTAAGCGCTGAACACGATCACGTCAAGACCCACCACGGTCATCACGA
TCATCACACTCACTTCCATATCGAAAACCACGGAAGCAAAGTTGCCTTAAGAACCCATTG
CGGTAAATACGTTTCAATTGGTGACCATAAACAAGTTTACCTCTCACACCACTTACACGG
TGACCACTCACTCTTCCACTTAGAACACCACCACGGTAAAGTCTCAATCAAAGGTCATCA
CCACCACTACATCTCTGTTGACGGTCACGGTCATGTCTCTACCTCCCATCACCACGATCA
TCATGCTACTTTCGAAGAACACATCTTATAAACAAATAAATAAAACAATAAAAATGTTGT
AAAATATTAAAAAATAAAAAAAAAA----------ATAAAAAACTTTTTATAAATAATAT
ATACATACAATGGGTAACAGAGCATTCAAAGCACACAACGGTCACTANTTAAGCGNTGAA
CACGATCACGTCAAGACCCACCACGGTCATCACGATCATCACACTCACTTCCATATCGAA
AACCACGGAAGCAAAGTTGCCTTAAGAACCCATTGCGGTAAATACGTTTCAATTGGTGAC
CATAAACAAGTTTACCTCTCACACCACTTACACGGTGACCACTCACTCTTCCANTTAGAA
CACCACCACGGTAAAGTNTCAATCAAAGGTCATCACCACCACTACATCTCTGTTGACGGT
CACGGTCATGTCTCNACCTCCCATCACCACGATCATCATGCTACTTTCGAAGAACACATC
TNANAAACAAATAAATAAAACAATAAAAATGTGTAAAATATTAAAAA
Length of connected seq. 877
Full length Seq ID -
Full length Seq. -
Length of full length seq. -