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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFO124
Library AF
(Link to library)
Clone ID AFO124
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15626-1
Original site URL
Representative seq. ID AFO124P
(Link to Original site)
Representative DNA sequence 
>AFO124 (AFO124Q) /CSM/AF/AFO1-A/AFO124Q.Seq.d/
TTTAAAATTAGATGAATTATTAAATNTTCAAGAATGNGAAAGNGTTAAAGTTGGAGCNCC
AGCNCATGAAGAGATGTTATTTATAATTATTCATCAAACTTATGAACTTTGGTTTAAACA
AANGATTCATGAAATCGATTCAATTCGTAGTXXXXXXXXXXATATGAAAGATCAATCAAC
CAAANAAGCTAAAAAGAATATGGAATNTTTNTCAACNCTTTTCGATGAAGTTGCTTATAA
TGAAAGATTAGAAAAAGGTGAAGTTAGATTAAGTTATAAAGCATTACAATCAGCAATTTT
AATTTATTTANATAAANATGAACCAATTTTCCANACNCCATTCCTTATTTTAAATCTTTT
AACAGAAATTGATGAATTATTAACAATGTGGAGATTCCGTCANACAATGATGGNACAAAG
AATTATTGGNGCCAAGATTGGNACTGGTGGNTNTTCTGGTTATCATTATTTACGGACAAC
CGTTGGTGATCGTTATAAAATTTTCTTGGATTTATTCAATATTTCCAGTTATTTAATTCC
NAAAAANACNCTTCCNCAATTACCNAAAGTTGTTAAAGAACAAANGGATTTCGCTTGGNC
AATTTTGNAATAAGTTTAAAAAAAAAAAAAAATAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
LKLDELLNXQEXEXVKVGAPAHEEMLFIIIHQTYELWFKQXIHEIDSIRS---

---MKDQSTKXAKKNMEXXSTLFDEVAYNERLEKGEVRLSYKALQSAILIYLXKXEPIFX
TPFLILNLLTEIDELLTMWRFRXTMMXQRIIGAKIGTGGXSGYHYLRTTVGDRYKIFLDL
FNISSYLIPKXTLPQLPKVVKEQXDFAWXILX*v*kkkki
Translated Amino Acid sequence (All Frames) 
Frame A:
fkir*iikxsrmxkx*swsxsx*rdviynyssnl*tlv*txds*nrfns*---

---i*kinqpxklkriwnxxqxfsmkllimkd*kkvkld*vikhynqqf*fixixmnqfs
xxhslf*if*qklmny*qcgdsvxq*wxkellxprlxlvxxlviiiygqplvivikfswi
ysifpvi*fxkxxfxnyxkllknkxislgqfxnkfkkkkk*

Frame B:
LKLDELLNXQEXEXVKVGAPAHEEMLFIIIHQTYELWFKQXIHEIDSIRS---

---yersinqxs*keygxfxnxfr*scl**kirkr*s*ikl*sitisnfnlfx*x*tnfp
xxipyfksfnrn**iinnveipsxndgtknywxqdwxwwxfwlslftdnrw*sl*nflgf
iqyfqlfnsxkxxsxitxsc*rtxgfrlxnfxislkkkkn

Frame C:
*n*mny*xfknxkxlklexqxmkrcyl*lfiklmnfglnkxfmksiqfv---

---MKDQSTKXAKKNMEXXSTLFDEVAYNERLEKGEVRLSYKALQSAILIYLXKXEPIFX
TPFLILNLLTEIDELLTMWRFRXTMMXQRIIGAKIGTGGXSGYHYLRTTVGDRYKIFLDL
FNISSYLIPKXTLPQLPKVVKEQXDFAWXILX*v*kkkki
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO124 (AFO124Q) /CSM/AF/AFO1-A/AFO124Q.Seq.d/                        997   0.0
SSH813 (SSH813Q) /CSM/SS/SSH8-A/SSH813Q.Seq.d/                        759   0.0
AFJ375 (AFJ375Q) /CSM/AF/AFJ3-D/AFJ375Q.Seq.d/                        751   0.0
AFN150 (AFN150Q) /CSM/AF/AFN1-C/AFN150Q.Seq.d/                        444   e-124
VSF818 (VSF818Q) /CSM/VS/VSF8-A/VSF818Q.Seq.d/                        355   5e-97
VHN627 (VHN627Q) /CSM/VH/VHN6-B/VHN627Q.Seq.d/                         36   0.63
VHA819 (VHA819Q) /CSM/VH/VHA8-A/VHA819Q.Seq.d/                         36   0.63
VFF723 (VFF723Q) /CSM/VF/VFF7-A/VFF723Q.Seq.d/                         36   0.63
SSE505 (SSE505Q) /CSM/SS/SSE5-A/SSE505Q.Seq.d/                         36   0.63
SHK167 (SHK167Q) /CSM/SH/SHK1-C/SHK167Q.Seq.d/                         36   0.63
own update 2009. 4. 4
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AI036267|AI036267.1 vl83f02.r1 Stratagene mouse diaphragm (#937303) Mus musculus cDNA clone IMAGE:987291 5' similar to SW:T23O_MOUSE P48776 TRYPTOPHAN 2,3-DIOXYGENASE ;, mRNA sequence.    56  5e-04  1
AA968298|AA968298.1 uc71g09.y1 Sugano mouse liver mlia Mus musculus cDNA clone IMAGE:1431136 5' similar to SW:T23O_MOUSE P48776 TRYPTOPHAN 2,3-DIOXYGENASE ;, mRNA sequence.    56  5e-04  1
AA986253|AA986253.1 uc74a10.y1 Sugano mouse liver mlia Mus musculus cDNA clone IMAGE:1431354 5' similar to SW:T23O_MOUSE P48776 TRYPTOPHAN 2,3-DIOXYGENASE ;, mRNA sequence.    56  5e-04  1
U24493|U24493.1 Mouse tryptophan-2,3-dioxygenase (TDO) mRNA, complete cds.    56  5e-04  1
AW012551|AW012551.1 uo06c12.y1 Sugano mouse liver mlia Mus musculus cDNA clone IMAGE:2582614 5' similar to SW:T23O_MOUSE P48776 TRYPTOPHAN 2,3-DIOXYGENASE ;, mRNA sequence.    56  5e-04  1
AA986058|AA986058.1 uc72e06.y1 Sugano mouse liver mlia Mus musculus cDNA clone IMAGE:1431202 5' similar to SW:T23O_MOUSE P48776 TRYPTOPHAN 2,3-DIOXYGENASE ;, mRNA sequence.    56  5e-04  1
AA570937|AA570937.1 vl85f03.r1 Stratagene mouse diaphragm (#937303) Mus musculus cDNA clone IMAGE:987485 5' similar to SW:T23O_MOUSE P48776 TRYPTOPHAN 2,3-DIOXYGENASE ;, mRNA sequence.    56  5e-04  1
AI048545|AI048545.1 ud61b09.y1 Sugano mouse liver mlia Mus musculus cDNA clone IMAGE:1450361 5' similar to SW:T23O_MOUSE P48776 TRYPTOPHAN 2,3-DIOXYGENASE ;, mRNA sequence.    56  5e-04  1
AI195493|AI195493.1 ui50f02.y1 Sugano mouse liver mlia Mus musculus cDNA clone IMAGE:1885851 5' similar to SW:T23O_MOUSE P48776 TRYPTOPHAN 2,3-DIOXYGENASE ;, mRNA sequence.    56  5e-04  1
BC018390|BC018390.1 Mus musculus, tryptophan 2,3-dioxygenase, clone MGC:25811 IMAGE:4159877, mRNA, complete cds.    56  5e-04  1
dna update 2003. 9.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q55DB4) RecName: Full=Tryptophan 2,3-dioxygenase;          Shor...   267   2e-70
(P48775) RecName: Full=Tryptophan 2,3-dioxygenase;          Shor...   144   2e-33
(Q7SY53) RecName: Full=Tryptophan 2,3-dioxygenase B;          Sh...   142   6e-33
U24493_1(U24493|pid:none) Mouse tryptophan-2,3-dioxygenase (TDO)...   142   6e-33
AY965263_1(AY965263|pid:none) Chlamys farreri tryptophan 2,3-dio...   142   6e-33
(P48776) RecName: Full=Tryptophan 2,3-dioxygenase;          Shor...   142   6e-33
(Q2KIQ5) RecName: Full=Tryptophan 2,3-dioxygenase;          Shor...   142   8e-33
(P21643) RecName: Full=Tryptophan 2,3-dioxygenase;          Shor...   142   8e-33
(Q5EBG2) RecName: Full=Tryptophan 2,3-dioxygenase;          Shor...   141   2e-32
(A7MBU6) RecName: Full=Tryptophan 2,3-dioxygenase A;          Sh...   132   8e-30
protein update 2009. 4.26
PSORT 

 psg: 0.75  gvh: 0.40  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.17  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for AFO124 is cyt
5' end seq. ID AFO124F
5' end seq. 
>AFO124F.Seq
TTTAAAATTAGATGAATTATTAAATNTTCAAGAATGNGAAAGNGTTAAAGTTGGAGCNCC
AGCNCATGAAGAGATGTTATTTATAATTATTCATCAAACTTATGAACTTTGGTTTAAACA
AANGATTCATGAAATCGATTCAATTCGTAGT----------
Length of 5' end seq. 151
3' end seq. ID AFO124Z
3' end seq. 
>AFO124Z.Seq
----------ATATGAAAGATCAATCAACCAAANAAGCTAAAAAGAATATGGAATNTTTN
TCAACNCTTTTCGATGAAGTTGCTTATAATGAAAGATTAGAAAAAGGTGAAGTTAGATTA
AGTTATAAAGCATTACAATCAGCAATTTTAATTTATTTANATAAANATGAACCAATTTTC
CANACNCCATTCCTTATTTTAAATCTTTTAACAGAAATTGATGAATTATTAACAATGTGG
AGATTCCGTCANACAATGATGGNACAAAGAATTATTGGNGCCAAGATTGGNACTGGTGGN
TNTTCTGGTTATCATTATTTACGGACAACCGTTGGTGATCGTTATAAAATTTTCTTGGAT
TTATTCAATATTTCCAGTTATTTAATTCCNAAAAANACNCTTCCNCAATTACCNAAAGTT
GTTAAAGAACAAANGGATTTCGCTTGGNCAATTTTGNAATAAGTTTAAAAAAAAAAAAAA
ATAA
Length of 3' end seq. 474
Connected seq. ID AFO124P
Connected seq. 
>AFO124P.Seq
TTTAAAATTAGATGAATTATTAAATNTTCAAGAATGNGAAAGNGTTAAAGTTGGAGCNCC
AGCNCATGAAGAGATGTTATTTATAATTATTCATCAAACTTATGAACTTTGGTTTAAACA
AANGATTCATGAAATCGATTCAATTCGTAGT----------ATATGAAAGATCAATCAAC
CAAANAAGCTAAAAAGAATATGGAATNTTTNTCAACNCTTTTCGATGAAGTTGCTTATAA
TGAAAGATTAGAAAAAGGTGAAGTTAGATTAAGTTATAAAGCATTACAATCAGCAATTTT
AATTTATTTANATAAANATGAACCAATTTTCCANACNCCATTCCTTATTTTAAATCTTTT
AACAGAAATTGATGAATTATTAACAATGTGGAGATTCCGTCANACAATGATGGNACAAAG
AATTATTGGNGCCAAGATTGGNACTGGTGGNTNTTCTGGTTATCATTATTTACGGACAAC
CGTTGGTGATCGTTATAAAATTTTCTTGGATTTATTCAATATTTCCAGTTATTTAATTCC
NAAAAANACNCTTCCNCAATTACCNAAAGTTGTTAAAGAACAAANGGATTTCGCTTGGNC
AATTTTGNAATAAGTTTAAAAAAAAAAAAAAATAA
Length of connected seq. 625
Full length Seq ID -
Full length Seq. -
Length of full length seq. -