>AFO122 (AFO122Q) /CSM/AF/AFO1-A/AFO122Q.Seq.d/
CACTGTTGGCCTACTGGTAATTTTTCTAATTTTTAATTTTTTTATATATATACATAAATA
TTTATTTTTATTTTTATTTTTTTTTATAATTTTCCAACATAACAATATCTAATTATAATT
TGGTATTAATAGTGTAATATTTGTTTTTTTTTTTTTTTAAAAAAGGGGGGAXXXXXXXXX
X

TVGLLVIFLIFNFFIYIHKYLFLFLFFFIIFQHNNI*l*fginsvifvffff*krg---

Frame A:
hcwptgnfsnf*ffyiyt*ififififfynfpt*qyliiiwy**cnicfffflkkg---


Frame B:
TVGLLVIFLIFNFFIYIHKYLFLFLFFFIIFQHNNI*l*fginsvifvffff*krg---


Frame C:
llayw*ff*fliflyiyiniyfyfyffl*fsnitisnynlvliv*ylfffffkkgg---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFN192 (VFN192Q) /CSM/VF/VFN1-D/VFN192Q.Seq.d/                        115   2e-25
VFN105 (VFN105Q) /CSM/VF/VFN1-A/VFN105Q.Seq.d/                        115   2e-25
VFL703 (VFL703Q) /CSM/VF/VFL7-A/VFL703Q.Seq.d/                        115   2e-25
VFJ870 (VFJ870Q) /CSM/VF/VFJ8-C/VFJ870Q.Seq.d/                        115   2e-25
VFJ151 (VFJ151Q) /CSM/VF/VFJ1-C/VFJ151Q.Seq.d/                        115   2e-25
VFA731 (VFA731Q) /CSM/VF/VFA7-B/VFA731Q.Seq.d/                        115   2e-25
SSC647 (SSC647Q) /CSM/SS/SSC6-B/SSC647Q.Seq.d/                        115   2e-25
SFI646 (SFI646Q) /CSM/SF/SFI6-B/SFI646Q.Seq.d/                        115   2e-25
SFG838 (SFG838Q) /CSM/SF/SFG8-B/SFG838Q.Seq.d/                        115   2e-25
CFK151 (CFK151Q) /CSM/CF/CFK1-C/CFK151Q.Seq.d/                        115   2e-25

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

L16578|L16578.1 Dictyostelium discoideum HIV1 TAT-binding protein homologous protein mRNA, complete cds.   115  2e-22  1
AP004388|AP004388.2 Homo sapiens genomic DNA, chromosome 8q23, clone:KB1239C7.    42  2.1  1
AF286886|AF286886.3 Homo sapiens chromosome 8 clone CTA-396G5 map 8q21-q23, complete sequence.    42  2.1  1
AC021237|AC021237.5 Homo sapiens chromosome 8, clone RP11-122A21, complete sequence.    42  2.1  1
AC102198|AC102198.3 Mus musculus clone RP24-499H13, WORKING DRAFT SEQUENCE, 6 unordered pieces.    42  2.1  1
BD099441|BD099441.1 Novel genes cloned in humanneuroblastoma and fragments thereof.    36  7.2  2
BD019503|BD019503.1 Novel gene and novel gene fragment cloned in human neuroblastoma.    36  7.2  2
BD099283|BD099283.1 Novel genes cloned in humanneuroblastoma and fragments thereof.    34  7.2  2
BD019345|BD019345.1 Novel gene and novel gene fragment cloned in human neuroblastoma.    34  7.2  2
AP003747|AP003747.4 Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 7, BAC clone:OJ1127_E01.    40  8.4  1

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3039690
Number of Hits to DB: 89,334,934

 psg: 1.01  gvh: 0.60  alm: 0.21  top: 0.57  tms: 0.07  mit: 0.31  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.28  leu: 0.14  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 44.0 %: nuclear
	 28.0 %: cytoplasmic
	  8.0 %: Golgi
	  8.0 %: mitochondrial
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for AFO122 is nuc

>AFO122F.Seq
CACTGTTGGCCTACTGGTAATTTTTCTAATTTTTAATTTTTTTATATATATACATAAATA
TTTATTTTTATTTTTATTTTTTTTTATAATTTTCCAACATAACAATATCTAATTATAATT
TGGTATTAATAGTGTAATATTTGTTTTTTTTTTTTTTTAAAAAAGGGGGGA---------
-