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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHG856
Library VH
(Link to library)
Clone ID VHG856
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11371-1
Original site URL
Representative seq. ID VHG856P
(Link to Original site)
Representative DNA sequence 
>VHG856 (VHG856Q) /CSM/VH/VHG8-C/VHG856Q.Seq.d/
AATNATCAACTAAATCAATAATAAACAATTAATCAAAAATGACAATCTTTGATAGCGACG
AAGAAGAGTTAGTTACTACTCTCAGTGATTTCGAACCACCAGTTCAAATTGATACTTATT
CAATAAATTTATTGTAGTTGATAATATTCCAGTTGCACCAGAGGCAAAACACGAGAAATT
GAAATCTATTTTAGTTAAAATCTATCAAAATAGACCAGGTGCTGAAGTTGTTGGTATTCA
TTTAGCATTAGATGCTCAAAGAAATACTAAAGGTTTTGCATTTATTGAATATTCAAAAAA
AGAATCAGCAATTGATGCAGCAACTTCATTAAATAATTATCCATTAGATAAATCACATAC
ATTAAAAGTTAATTTATTAGATGATTTTAGCAAATTTGCCAATTTTGAAGAAACTTATAA
ACCACCAACTAGAGAAGATTTTAAACCAAAACCAAATTACAATCAATGGTTATCAGATCC
AAAAGCATTGAAAGGTTATGACCAATTTGTAACACGTTATGGTGATTATACAGATATTTG
TTGGAATGAGATGCATGTTGGTAAACCAATGGTAGAGAAATCATCGGTTTCATTGACCTC
ATCATACGTTCAATGGTCAAACACTGGTAGTTATTTAGTTACXXXXXXXXXXAAAACAAG
TGTTAAACTCATTAGTAAATTAGAGAATCGTAAATTGAATACCATCTTTTGGTCACCACG
TGGTACTTTGGTATTATTGGCCAATTTGGGTGACACTGGTGAATTGGAATTCTACAACAC
TCAAGACTGTGAATCCTATGGCACTCAAGAACATCTCCAATGTACTGGTGTCGATTGGAA
TCCATCTGGTCGTTATGTTACCACCTTTGTTAGTCATTGGAAGGTTCAAACCGATACTGG
TTTCAACATTTGGTCATTCAATGGTGATCTCGTCTACTCTGTACTCAAGGATCGTTTCTT
CCAATTCAATTGGCGTCCAAGACCAAAATTCATGCTCACCAATAAAGAAATCAATCAAAT
CAAACAAAACATCAAAAAATATCAAGAGAAATTCGATAAACAAGATGAAGATGATAATAA
AGCCATTCAACATATTGAACAAACTCGTCTTGATAATTTAATGAAAGAATTTTTATCTTT
CCTTCAAAAAGGTGAACAAGAATATCAAGCACTCGAACCAACTAGAAAACAACTTGGTTC
TTATGAAGATATTGATCCAAATGATATCTATGAATCAATGAAACAATGAAGAACTTATNA
TATTAAAACTATNTTTTAAAGAAAAAAAATNAAATT
sequence update 2002.10.25
Translated Amino Acid sequence 
xin*innkqlikndnl**rrrrvsyysq*frttssn*YLFNKFIVVDNIPVAPEAKHEKL
KSILVKIYQNRPGAEVVGIHLALDAQRNTKGFAFIEYSKKESAIDAATSLNNYPLDKSHT
LKVNLLDDFSKFANFEETYKPPTREDFKPKPNYNQWLSDPKALKGYDQFVTRYGDYTDIC
WNEMHVGKPMVEKSSVSLTSSYVQWSNTGSYLV---

---KTSVKLISKLENRKLNTIFWSPRGTLVLLANLGDTGELEFYNTQDCESYGTQEHLQC
TGVDWNPSGRYVTTFVSHWKVQTDTGFNIWSFNGDLVYSVLKDRFFQFNWRPRPKFMLTN
KEINQIKQNIKKYQEKFDKQDEDDNKAIQHIEQTRLDNLMKEFLSFLQKGEQEYQALEPT
RKQLGSYEDIDPNDIYESMKQ*rtyxiktxf*rkkxk
Translated Amino Acid sequence (All Frames) 
Frame A:
nxqlnq**tinqk*qsliatkks*lllsvisnhqfkliliq*iycs**yssctrgktrei
eiyfs*nlsk*trc*scwysfsircskky*rfciy*ifkkrisn*csnfik*lsir*ity
iks*fir*f*qicqf*rnl*ttn*rrf*tktklqsmvirsksierl*picntlw*lyryl
le*dacw*tngreiigfidliirsmvkhw*lfsy---

---KTSVKLISKLENRKLNTIFWSPRGTLVLLANLGDTGELEFYNTQDCESYGTQEHLQC
TGVDWNPSGRYVTTFVSHWKVQTDTGFNIWSFNGDLVYSVLKDRFFQFNWRPRPKFMLTN
KEINQIKQNIKKYQEKFDKQDEDDNKAIQHIEQTRLDNLMKEFLSFLQKGEQEYQALEPT
RKQLGSYEDIDPNDIYESMKQ*rtyxiktxf*rkkxk

Frame B:
xin*innkqlikndnl**rrrrvsyysq*frttssn*YLFNKFIVVDNIPVAPEAKHEKL
KSILVKIYQNRPGAEVVGIHLALDAQRNTKGFAFIEYSKKESAIDAATSLNNYPLDKSHT
LKVNLLDDFSKFANFEETYKPPTREDFKPKPNYNQWLSDPKALKGYDQFVTRYGDYTDIC
WNEMHVGKPMVEKSSVSLTSSYVQWSNTGSYLV---

---kqvlnslvn*rivn*ipsfghhvvlwyywpiwvtlvnwnsttlktvnpmalknisnv
lvsigihlvvmlppllvigrfkpilvstfghsmvisstlysrivssnsigvqdqnscspi
kksiksnktsknikrnsinkmkmiikpfnilnklvlii**knfylsfkkvnknikhsnql
ennlvlmkiliqmismnq*nneelxilklxfkekkxn

Frame C:
xstksiinn*skmtifdsdeeelvttlsdfeppvqidtysinll*liifqlhqrqntrn*
nlf*lksikidqvlkllvfi*h*mlkeilkvlhllniqkknqqlmqqlh*iiih*inhih
*kliy*milanlpilkklinhqlekilnqnqitingyqiqkh*kvmtnl*hvmviiqifv
gmrcmlvnqw*rnhrfh*phhtfngqtlvvi*l---

---nkc*th**ires*ieyhllvttwyfgiigqfg*hw*igilqhsrl*ilwhsrtspmy
wcrlesiwslcyhlc*slegsnrywfqhlviqw*srllctqgsflpiqlasktkihahq*
rnqsnqtkhqkisreir*tr*r***shsty*tnss**fnerififpskr*trisstrtn*
kttwfl*ry*sk*yl*inetmknlxy*nyxlkkknxi
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHG856 (VHG856Q) /CSM/VH/VHG8-C/VHG856Q.Seq.d/                       1889   0.0
VFK194 (VFK194Q) /CSM/VF/VFK1-D/VFK194Q.Seq.d/                       1211   0.0
SFJ772 (SFJ772Q) /CSM/SF/SFJ7-C/SFJ772Q.Seq.d/                       1193   0.0
SFE596 (SFE596Q) /CSM/SF/SFE5-D/SFE596Q.Seq.d/                       1193   0.0
VHF256 (VHF256Q) /CSM/VH/VHF2-C/VHF256Q.Seq.d/                       1187   0.0
VHE819 (VHE819Q) /CSM/VH/VHE8-A/VHE819Q.Seq.d/                       1183   0.0
VFA136 (VFA136Q) /CSM/VF/VFA1-B/VFA136Q.Seq.d/                       1178   0.0
CHE725 (CHE725Q) /CSM/CH/CHE7-B/CHE725Q.Seq.d/                       1162   0.0
VHE503 (VHE503Q) /CSM/VH/VHE5-A/VHE503Q.Seq.d/                       1158   0.0
SLC263 (SLC263Q) /CSM/SL/SLC2-C/SLC263Q.Seq.d/                       1146   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ422063) Dictyostelium discoideum cDNA clone:ddv44p14, 5' ...   676   0.0    2
(BJ415230) Dictyostelium discoideum cDNA clone:ddv21k23, 5' ...   676   0.0    3
(BJ410645) Dictyostelium discoideum cDNA clone:ddv1g09, 5' e...   676   0.0    3
(BJ421094) Dictyostelium discoideum cDNA clone:ddv41o14, 5' ...   668   0.0    4
(BJ420672) Dictyostelium discoideum cDNA clone:ddv40f06, 5' ...   664   0.0    4
(BJ386847) Dictyostelium discoideum cDNA clone:dds10o23, 5' ...   664   0.0    3
(BJ389969) Dictyostelium discoideum cDNA clone:dds20p17, 5' ...   658   0.0    3
(BJ440781) Dictyostelium discoideum cDNA clone:ddv44p14, 3' ...   656   0.0    2
(BJ378237) Dictyostelium discoideum cDNA clone:ddc31b07, 3' ...   656   0.0    2
(BJ402173) Dictyostelium discoideum cDNA clone:dds20p17, 3' ...   648   0.0    2
dna update 2008.11. 5
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EF660343_1(EF660343|pid:none) Artemisia annua eukaryotic transla...   132   2e-29
AF255680_1(AF255680|pid:none) Arabidopsis thaliana eIF3b mRNA, c...   129   2e-28
BT002393_1(BT002393|pid:none) Arabidopsis thaliana eukaryotic tr...   129   2e-28
(Q1HDZ5) RecName: Full=Eukaryotic translation initiation factor ...   128   5e-28
CR954215_188(CR954215|pid:none) Ostreococcus tauri strain OTTH05...   127   7e-28
BT064159_1(BT064159|pid:none) Zea mays full-length cDNA clone ZM...   127   9e-28
AC137830_32(AC137830|pid:none) Medicago truncatula clone mth2-33...   127   1e-27
(Q9C5Z1) RecName: Full=Eukaryotic translation initiation factor ...   125   4e-27
(B0W562) RecName: Full=Eukaryotic translation initiation factor ...   117   9e-25
(Q4P6G4) RecName: Full=Eukaryotic translation initiation factor ...   115   3e-24
protein update 2009. 7.12
PSORT 

 psg: 0.71  gvh: 0.39  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.33
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 32.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for VHG856 is nuc
5' end seq. ID VHG856F
5' end seq. 
>VHG856F.Seq
AATNATCAACTAAATCAATAATAAACAATTAATCAAAAATGACAATCTTTGATAGCGACG
AAGAAGAGTTAGTTACTACTCTCAGTGATTTCGAACCACCAGTTCAAATTGATACTTATT
CAATAAATTTATTGTAGTTGATAATATTCCAGTTGCACCAGAGGCAAAACACGAGAAATT
GAAATCTATTTTAGTTAAAATCTATCAAAATAGACCAGGTGCTGAAGTTGTTGGTATTCA
TTTAGCATTAGATGCTCAAAGAAATACTAAAGGTTTTGCATTTATTGAATATTCAAAAAA
AGAATCAGCAATTGATGCAGCAACTTCATTAAATAATTATCCATTAGATAAATCACATAC
ATTAAAAGTTAATTTATTAGATGATTTTAGCAAATTTGCCAATTTTGAAGAAACTTATAA
ACCACCAACTAGAGAAGATTTTAAACCAAAACCAAATTACAATCAATGGTTATCAGATCC
AAAAGCATTGAAAGGTTATGACCAATTTGTAACACGTTATGGTGATTATACAGATATTTG
TTGGAATGAGATGCATGTTGGTAAACCAATGGTAGAGAAATCATCGGTTTCATTGACCTC
ATCATACGTTCAATGGTCAAACACTGGTAGTTATTTAGTTACNNNNNNNNNN
Length of 5' end seq. 652
3' end seq. ID VHG856Z
3' end seq. 
>VHG856Z.Seq
NNNNNNNNNNAAAACAAGTGTTAAACTCATTAGTAAATTAGAGAATCGTAAATTGAATAC
CATCTTTTGGTCACCACGTGGTACTTTGGTATTATTGGCCAATTTGGGTGACACTGGTGA
ATTGGAATTCTACAACACTCAAGACTGTGAATCCTATGGCACTCAAGAACATCTCCAATG
TACTGGTGTCGATTGGAATCCATCTGGTCGTTATGTTACCACCTTTGTTAGTCATTGGAA
GGTTCAAACCGATACTGGTTTCAACATTTGGTCATTCAATGGTGATCTCGTCTACTCTGT
ACTCAAGGATCGTTTCTTCCAATTCAATTGGCGTCCAAGACCAAAATTCATGCTCACCAA
TAAAGAAATCAATCAAATCAAACAAAACATCAAAAAATATCAAGAGAAATTCGATAAACA
AGATGAAGATGATAATAAAGCCATTCAACATATTGAACAAACTCGTCTTGATAATTTAAT
GAAAGAATTTTTATCTTTCCTTCAAAAAGGTGAACAAGAATATCAAGCACTCGAACCAAC
TAGAAAACAACTTGGTTCTTATGAAGATATTGATCCAAATGATATCTATGAATCAATGAA
ACAATGAAGAACTTATNATATTAAAACTATNTTTTAAAGAAAAAAAATNAAATT
Length of 3' end seq. 654
Connected seq. ID VHG856P
Connected seq. 
>VHG856P.Seq
AATNATCAACTAAATCAATAATAAACAATTAATCAAAAATGACAATCTTTGATAGCGACG
AAGAAGAGTTAGTTACTACTCTCAGTGATTTCGAACCACCAGTTCAAATTGATACTTATT
CAATAAATTTATTGTAGTTGATAATATTCCAGTTGCACCAGAGGCAAAACACGAGAAATT
GAAATCTATTTTAGTTAAAATCTATCAAAATAGACCAGGTGCTGAAGTTGTTGGTATTCA
TTTAGCATTAGATGCTCAAAGAAATACTAAAGGTTTTGCATTTATTGAATATTCAAAAAA
AGAATCAGCAATTGATGCAGCAACTTCATTAAATAATTATCCATTAGATAAATCACATAC
ATTAAAAGTTAATTTATTAGATGATTTTAGCAAATTTGCCAATTTTGAAGAAACTTATAA
ACCACCAACTAGAGAAGATTTTAAACCAAAACCAAATTACAATCAATGGTTATCAGATCC
AAAAGCATTGAAAGGTTATGACCAATTTGTAACACGTTATGGTGATTATACAGATATTTG
TTGGAATGAGATGCATGTTGGTAAACCAATGGTAGAGAAATCATCGGTTTCATTGACCTC
ATCATACGTTCAATGGTCAAACACTGGTAGTTATTTAGTTAC----------AAAACAAG
TGTTAAACTCATTAGTAAATTAGAGAATCGTAAATTGAATACCATCTTTTGGTCACCACG
TGGTACTTTGGTATTATTGGCCAATTTGGGTGACACTGGTGAATTGGAATTCTACAACAC
TCAAGACTGTGAATCCTATGGCACTCAAGAACATCTCCAATGTACTGGTGTCGATTGGAA
TCCATCTGGTCGTTATGTTACCACCTTTGTTAGTCATTGGAAGGTTCAAACCGATACTGG
TTTCAACATTTGGTCATTCAATGGTGATCTCGTCTACTCTGTACTCAAGGATCGTTTCTT
CCAATTCAATTGGCGTCCAAGACCAAAATTCATGCTCACCAATAAAGAAATCAATCAAAT
CAAACAAAACATCAAAAAATATCAAGAGAAATTCGATAAACAAGATGAAGATGATAATAA
AGCCATTCAACATATTGAACAAACTCGTCTTGATAATTTAATGAAAGAATTTTTATCTTT
CCTTCAAAAAGGTGAACAAGAATATCAAGCACTCGAACCAACTAGAAAACAACTTGGTTC
TTATGAAGATATTGATCCAAATGATATCTATGAATCAATGAAACAATGAAGAACTTATNA
TATTAAAACTATNTTTTAAAGAAAAAAAATNAAATT
Length of connected seq. 1286
Full length Seq ID -
Full length Seq. -
Length of full length seq. -