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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHG777
Library VH
(Link to library)
Clone ID VHG777
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16430-1
Original site URL
Representative seq. ID VHG777P
(Link to Original site)
Representative DNA sequence 
>VHG777 (VHG777Q) /CSM/VH/VHG7-D/VHG777Q.Seq.d/
ACATTTAGGTCCTATATTCTAATTCATAATTAATTTTATTTGTTTTTTTTTGTANAACAT
AAAAAAATGGCTGAATCACAAGTTGAACGTTTCACATTCCAAGCTGAAATTAATCAGCTT
ATGTCCCTTATTATTAATACTTTTTATTCAAATAAAGAAGTATTTTTAAGAGAATTGATT
TCAAATGCATCTGATGCCTTANATAAAATTCGTTACCAATCATTAACTGATGCATCAGTT
TTGGAATCAAAAACTGAACTCGAAATTAAAATTATTCCAGATAAGACTGCCAAAACTCTT
ACACTCATTGATAGTGGTATTGGTATGACCAAAACCGATATGGTAAAGAATTTAGGTACT
ATTGCTCGTTCAGGTACTAAAAACTTTATGGAACAATTACAAAGTGGTGCCGCTGATATC
AGTATGATTGGTCAATTTGGTGTTGGTTTCTACTCTGCTTACTTAGTTGCCGATACTGTT
ATTGTTCACTCTAAAAACAATGATGATGAACXXXXXXXXXXAGCTGCCAATGAAGAATTA
CTCAAACAAGTTAAAGATGTATTGGGTGATAAAGTTGAAAAGGTAGTTCTCTCAACTCGT
CTTGCCAACTCACCATGTGTTTTAGTTACCTCTGAATATGGTTGGTCAGCAAACATGGAA
CGTATCATGAAAGCTCAAGCTCTCCGTGACTCTTCAATGTCATCATACATGTCATCAAAG
AAAACTTTAGAATTAAATCCAGATCACCCAATCGTTAGAGATTTAGCCAAAAAAGCCGCC
GAAAAAAGTAAAACTTTCAAAGACTTTGTTTACTTATTATATGAAACTGCTCTCTTAACT
TCAGGTTTCTCATTAGATGAACCATCATCATTTGCTTCACGTATTCATCGTATGATTAAA
TTAGGTTTAAGCATTCAAGATGATTCATCAGCAACAACTGAAGAATCAACAAACACAACA
ACCAGTGATGATATTCCACCATTAGAAGAAAACGATGAACCATCTGAAAGGAAAAAGTCG
ACTAGATTTAATTACTAGCCCTTTTGCTATCAATTTTTGGAATATTGTTATATTATTATA
ATAAAATAAATTTTAAAAAAAATCTTCA
sequence update 2002.10.25
Translated Amino Acid sequence 
tfrsyilihn*FYLFFFVXHKKMAESQVERFTFQAEINQLMSLIINTFYSNKEVFLRELI
SNASDALXKIRYQSLTDASVLESKTELEIKIIPDKTAKTLTLIDSGIGMTKTDMVKNLGT
IARSGTKNFMEQLQSGAADISMIGQFGVGFYSAYLVADTVIVHSKNNDDE---

---AANEELLKQVKDVLGDKVEKVVLSTRLANSPCVLVTSEYGWSANMERIMKAQALRDS
SMSSYMSSKKTLELNPDHPIVRDLAKKAAEKSKTFKDFVYLLYETALLTSGFSLDEPSSF
ASRIHRMIKLGLSIQDDSSATTEESTNTTTSDDIPPLEENDEPSERKKSTRFNY*pfcyq
fleycyiiiik*ilkkif
Translated Amino Acid sequence (All Frames) 
Frame A:
tfrsyilihn*FYLFFFVXHKKMAESQVERFTFQAEINQLMSLIINTFYSNKEVFLRELI
SNASDALXKIRYQSLTDASVLESKTELEIKIIPDKTAKTLTLIDSGIGMTKTDMVKNLGT
IARSGTKNFMEQLQSGAADISMIGQFGVGFYSAYLVADTVIVHSKNNDDE---

---scq*ritqts*rcig**s*kgsslnsscqltmcfsyl*iwlvskhgtyhessssp*l
fnviihvikenfriksrspnr*rfsqksrrkk*nfqrlcllii*ncslnfrflir*tiii
cftyssyd*irfkhsr*fisnn*rinkhnnq**ystirrkr*ti*kekvd*i*llallls
ifgillyyynkinfkknl

Frame B:
hlgpif*fiinficfflxnikkwlnhklnvshsklklislcplllilfiqikkyf*en*f
qmhlmpxikfvtnh*lmhqfwnqklnsklklfqirlpkllhslivvlv*pkpiw*ri*vl
llvqvlktlwnnykvvplisv*lvnlvlvstllt*lpilllftlktmmmn---

---AANEELLKQVKDVLGDKVEKVVLSTRLANSPCVLVTSEYGWSANMERIMKAQALRDS
SMSSYMSSKKTLELNPDHPIVRDLAKKAAEKSKTFKDFVYLLYETALLTSGFSLDEPSSF
ASRIHRMIKLGLSIQDDSSATTEESTNTTTSDDIPPLEENDEPSERKKSTRFNY*pfcyq
fleycyiiiik*ilkkif

Frame C:
i*vlysns*lilfvffcxt*kng*its*tfhips*n*sayvpyy*yflfk*rsifkridf
kci*clx*nslpiin*cisfgikn*trn*nysr*dcqnsyth**wywydqnrygkefryy
csfry*klygtitkwcr*yqydwsiwcwfllcllscrycycsl*kq***---

---lpmknysnklkmywviklkr*fsqlvlpthhvf*lplnmvgqqtwnvs*klklsvtl
qchhtchqrkl*n*iqitqslei*pkkppkkvklsktlftyymklls*lqvsh*mnhhhl
lhvfiv*ln*v*afkmihqqqlknqqtqqpvmifhh*kktmnhlkgksrldlitspfain
fwnivill**nkf*kkss
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHQ442 (VHQ442Q) /CSM/VH/VHQ4-B/VHQ442Q.Seq.d/                        874   0.0
VHP240 (VHP240Q) /CSM/VH/VHP2-B/VHP240Q.Seq.d/                        874   0.0
VHP202 (VHP202Q) /CSM/VH/VHP2-A/VHP202Q.Seq.d/                        874   0.0
VHP152 (VHP152Q) /CSM/VH/VHP1-C/VHP152Q.Seq.d/                        874   0.0
VHP148 (VHP148Q) /CSM/VH/VHP1-B/VHP148Q.Seq.d/                        874   0.0
VHO403 (VHO403Q) /CSM/VH/VHO4-A/VHO403Q.Seq.d/                        874   0.0
VHO391 (VHO391Q) /CSM/VH/VHO3-D/VHO391Q.Seq.d/                        874   0.0
VHN791 (VHN791Q) /CSM/VH/VHN7-D/VHN791Q.Seq.d/                        874   0.0
VHN633 (VHN633Q) /CSM/VH/VHN6-B/VHN633Q.Seq.d/                        874   0.0
VHN558 (VHN558Q) /CSM/VH/VHN5-C/VHN558Q.Seq.d/                        874   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(L43591) Dictyostelium discoideum (clone C 6.1.1) heat shock...   874   0.0    4
(BJ446614) Dictyostelium discoideum cDNA clone:ddv63d12, 3' ...   597   0.0    2
(BJ443243) Dictyostelium discoideum cDNA clone:ddv52h09, 3' ...   597   0.0    2
(BJ442800) Dictyostelium discoideum cDNA clone:ddv51b06, 3' ...   597   0.0    2
(BJ440833) Dictyostelium discoideum cDNA clone:ddv44j19, 3' ...   597   0.0    2
(BJ440298) Dictyostelium discoideum cDNA clone:ddv43b10, 3' ...   597   0.0    2
(BJ437296) Dictyostelium discoideum cDNA clone:ddv33f22, 3' ...   597   0.0    2
(BJ381861) Dictyostelium discoideum cDNA clone:ddc42l23, 3' ...   597   0.0    2
(BJ357655) Dictyostelium discoideum cDNA clone:dda64d07, 3' ...   597   0.0    2
(BJ357079) Dictyostelium discoideum cDNA clone:dda62k10, 3' ...   597   0.0    2
dna update 2008.11. 5
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P54651) RecName: Full=Heat shock cognate 90 kDa protein;             281   3e-74
L43591_1(L43591|pid:none) Dictyostelium discoideum (clone C 6.1....   281   3e-74
AB300196_1(AB300196|pid:none) Physarum polycephalum hsp90 mRNA f...   229   1e-58
EF687776_1(EF687776|pid:none) Crassostrea gigas heat shock prote...   220   6e-56
U39048_1(U39048|pid:none) Achlya ambisexualis heat shock protein...   218   2e-55
U39047_1(U39047|pid:none) Achlya ambisexualis heat shock protein...   218   2e-55
AF402101_1(AF402101|pid:none) Achlya ambisexualis isolate JS855 ...   218   2e-55
AF402100_1(AF402100|pid:none) Achlya ambisexualis isolate JS854 ...   218   2e-55
BT063272_1(BT063272|pid:none) Zea mays full-length cDNA clone ZM...   217   7e-55
EF532406_1(EF532406|pid:none) Argopecten irradians heat shock pr...   216   9e-55
protein update 2009. 7.12
PSORT 

 psg: 0.50  gvh: 0.45  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.16  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 36.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for VHG777 is nuc
5' end seq. ID VHG777F
5' end seq. 
>VHG777F.Seq
ACATTTAGGTCCTATATTCTAATTCATAATTAATTTTATTTGTTTTTTTTTGTANAACAT
AAAAAAATGGCTGAATCACAAGTTGAACGTTTCACATTCCAAGCTGAAATTAATCAGCTT
ATGTCCCTTATTATTAATACTTTTTATTCAAATAAAGAAGTATTTTTAAGAGAATTGATT
TCAAATGCATCTGATGCCTTANATAAAATTCGTTACCAATCATTAACTGATGCATCAGTT
TTGGAATCAAAAACTGAACTCGAAATTAAAATTATTCCAGATAAGACTGCCAAAACTCTT
ACACTCATTGATAGTGGTATTGGTATGACCAAAACCGATATGGTAAAGAATTTAGGTACT
ATTGCTCGTTCAGGTACTAAAAACTTTATGGAACAATTACAAAGTGGTGCCGCTGATATC
AGTATGATTGGTCAATTTGGTGTTGGTTTCTACTCTGCTTACTTAGTTGCCGATACTGTT
ATTGTTCACTCTAAAAACAATGATGATGAACNNNNNNNNNN
Length of 5' end seq. 521
3' end seq. ID VHG777Z
3' end seq. 
>VHG777Z.Seq
NNNNNNNNNNAGCTGCCAATGAAGAATTACTCAAACAAGTTAAAGATGTATTGGGTGATA
AAGTTGAAAAGGTAGTTCTCTCAACTCGTCTTGCCAACTCACCATGTGTTTTAGTTACCT
CTGAATATGGTTGGTCAGCAAACATGGAACGTATCATGAAAGCTCAAGCTCTCCGTGACT
CTTCAATGTCATCATACATGTCATCAAAGAAAACTTTAGAATTAAATCCAGATCACCCAA
TCGTTAGAGATTTAGCCAAAAAAGCCGCCGAAAAAAGTAAAACTTTCAAAGACTTTGTTT
ACTTATTATATGAAACTGCTCTCTTAACTTCAGGTTTCTCATTAGATGAACCATCATCAT
TTGCTTCACGTATTCATCGTATGATTAAATTAGGTTTAAGCATTCAAGATGATTCATCAG
CAACAACTGAAGAATCAACAAACACAACAACCAGTGATGATATTCCACCATTAGAAGAAA
ACGATGAACCATCTGAAAGGAAAAAGTCGACTAGATTTAATTACTAGCCCTTTTGCTATC
AATTTTTGGAATATTGTTATATTATTATAATAAAATAAATTTTAAAAAAAATCTTCA
Length of 3' end seq. 597
Connected seq. ID VHG777P
Connected seq. 
>VHG777P.Seq
ACATTTAGGTCCTATATTCTAATTCATAATTAATTTTATTTGTTTTTTTTTGTANAACAT
AAAAAAATGGCTGAATCACAAGTTGAACGTTTCACATTCCAAGCTGAAATTAATCAGCTT
ATGTCCCTTATTATTAATACTTTTTATTCAAATAAAGAAGTATTTTTAAGAGAATTGATT
TCAAATGCATCTGATGCCTTANATAAAATTCGTTACCAATCATTAACTGATGCATCAGTT
TTGGAATCAAAAACTGAACTCGAAATTAAAATTATTCCAGATAAGACTGCCAAAACTCTT
ACACTCATTGATAGTGGTATTGGTATGACCAAAACCGATATGGTAAAGAATTTAGGTACT
ATTGCTCGTTCAGGTACTAAAAACTTTATGGAACAATTACAAAGTGGTGCCGCTGATATC
AGTATGATTGGTCAATTTGGTGTTGGTTTCTACTCTGCTTACTTAGTTGCCGATACTGTT
ATTGTTCACTCTAAAAACAATGATGATGAAC----------AGCTGCCAATGAAGAATTA
CTCAAACAAGTTAAAGATGTATTGGGTGATAAAGTTGAAAAGGTAGTTCTCTCAACTCGT
CTTGCCAACTCACCATGTGTTTTAGTTACCTCTGAATATGGTTGGTCAGCAAACATGGAA
CGTATCATGAAAGCTCAAGCTCTCCGTGACTCTTCAATGTCATCATACATGTCATCAAAG
AAAACTTTAGAATTAAATCCAGATCACCCAATCGTTAGAGATTTAGCCAAAAAAGCCGCC
GAAAAAAGTAAAACTTTCAAAGACTTTGTTTACTTATTATATGAAACTGCTCTCTTAACT
TCAGGTTTCTCATTAGATGAACCATCATCATTTGCTTCACGTATTCATCGTATGATTAAA
TTAGGTTTAAGCATTCAAGATGATTCATCAGCAACAACTGAAGAATCAACAAACACAACA
ACCAGTGATGATATTCCACCATTAGAAGAAAACGATGAACCATCTGAAAGGAAAAAGTCG
ACTAGATTTAATTACTAGCCCTTTTGCTATCAATTTTTGGAATATTGTTATATTATTATA
ATAAAATAAATTTTAAAAAAAATCTTCA
Length of connected seq. 1098
Full length Seq ID -
Full length Seq. -
Length of full length seq. -