>AFO108 (AFO108Q) /CSM/AF/AFO1-A/AFO108Q.Seq.d/
ATTTTTTATTTTATTTTCCAATTGATAAAATTAAATTAAATAAAATGTCATTAAATATTG
AAAAAGTTAAAGAATTTAGTAATAAAGCATGGGATGAAAATATTTTACCAACACTTTCAA
AATATATTGAAATTCCAAATCAATCACCATTATATGATGCAGAATGGGCAACCAATGGTT
ATACAGAAGAAGCAATTCAATTATTAGAGAAATGGATCTTAGCACAAGGTATCAAAGGTA
TTTCATGTGAAATTAAAAGAATTGAAGGATTAACACCAATTATTTTCATGGTAGTCGAAG
CAACCAAAACTGAAAAAGTTAAAACTGTACTTTTATATGGTCACATGGATAAACAACCAC
CACTCACTGATGCATGGGATGAAGGATTACATCCATACAAAGCAGTCATCAAGAATAACA
AACTCTATGGTCGTGGTGGTGCAGATGATGGTTATTCAACATTTGCTTCAATCTCTGCAA
TCAAAGCATTGCAAGAACAATCAATTCCACATGATCGTTATGTAATTATCATTGAAGGTT
CCGAAGAATCTGGTTCAATCCATTTACCACAATATGTTGAGAAGTTCCAAGCTGAAATTC
AAATTCXXXXXXXXXXAATGAAGCATGTCATTCCGGTAGTGCNTCTGGTATCGTTCCATC
TTCATTCCGTATTCTTCGTCAAGTTTTAGATCGTGCTGAAAATCAATTAACTGGTGAAGT
CGATAAAAGATTACACGTTGAAATTCCACAACATCGTTTAGATCAAATCGCAAAATGTGC
TGAAATTTTAGGCGAAACCATCTATGATGAATTCTCATGGTACAAAAATACCACTCCAGT
CACTAAAGATTTAACCCAATTGATGATCAACAAAACTTGGTATCCAACTATTTGCATCAC
CGGTGCTGAAGGTATTCCACACACTTCAAATGCTGGTAATGTCATGAGAACTCACACCAC
CGTTAAAGTTAGTGTTCGTTTACCACCATCATTCAAAGCAGCTGATGCATCAACTGTATT
AAAAGAAATTTTCGAAACTAATCCACCATATGGTGCTGAAGTTACCTTCAAAATGGATAA
ATGTGCTTCAGGTTGGGATTCACCACAAACTAGACCAGAATTAGAAAAAGCATTAGGAGA
AGCTTCAAATCTTTTCTTTGGCAAAGACCACGTATACATGAGTGAAGGTGGTTCCATCCC
ATTCATGAAAATGTTACATGACCGTTTCAATTGTCAATTTATCGTTACTGGTTTATTAGG
TCCAGGTTCAAATGCTCATGGTCCAAATGAATTCTTAGATATTCCATTTGCCAAGAAATT
ATTAAGTTGTGTTATTTATTTATTAGATGTCTCANTGCCAAGTTNNATTAAATAAA

FLFYFPIDKIKLNKMSLNIEKVKEFSNKAWDENILPTLSKYIEIPNQSPLYDAEWATNGY
TEEAIQLLEKWILAQGIKGISCEIKRIEGLTPIIFMVVEATKTEKVKTVLLYGHMDKQPP
LTDAWDEGLHPYKAVIKNNKLYGRGGADDGYSTFASISAIKALQEQSIPHDRYVIIIEGS
EESGSIHLPQYVEKFQAEIQI---

---NEACHSGSASGIVPSSFRILRQVLDRAENQLTGEVDKRLHVEIPQHRLDQIAKCAEI
LGETIYDEFSWYKNTTPVTKDLTQLMINKTWYPTICITGAEGIPHTSNAGNVMRTHTTVK
VSVRLPPSFKAADASTVLKEIFETNPPYGAEVTFKMDKCASGWDSPQTRPELEKALGEAS
NLFFGKDHVYMSEGGSIPFMKMLHDRFNCQFIVTGLLGPGSNAHGPNEFLDIPFAKKLLS
CVIYLLDVSXPSXIK*

Frame A:
ifyfifqlikln*ikch*ilkklknlvikhgmkifyqhfqnilkfqinhhymmqngqpmv
iqkkqfny*rngs*hkvskvfhvklkelkd*hqlfsw*skqpklkklklyfymvtwinnh
hslmhgmkdyihtkqssritnsmvvvvqmmviqhllqslqskhcknnqfhmivm*lslkv
pknlvqsiyhnmlrssklkfkf---

---NEACHSGSASGIVPSSFRILRQVLDRAENQLTGEVDKRLHVEIPQHRLDQIAKCAEI
LGETIYDEFSWYKNTTPVTKDLTQLMINKTWYPTICITGAEGIPHTSNAGNVMRTHTTVK
VSVRLPPSFKAADASTVLKEIFETNPPYGAEVTFKMDKCASGWDSPQTRPELEKALGEAS
NLFFGKDHVYMSEGGSIPFMKMLHDRFNCQFIVTGLLGPGSNAHGPNEFLDIPFAKKLLS
CVIYLLDVSXPSXIK*

Frame B:
ffilfsn**n*ik*nviky*ks*ri***smg*kyftntfkiy*nsksitii*crmgnqwl
yrrsnsiiremdlstryqryfm*n*kn*rintnyfhgsrsnqn*ks*nctfiwshg*ttt
th*cmg*ritsiqsshqe*qtlwswwcr*wlfnicfnlcnqsiartinst*slcnyh*rf
rriwfnpfttic*evps*nsn---

---mkhvipvvxlvsfhlhsvffvkf*ivlkin*lvksikdytlkfhniv*iksqnvlkf
*akpsmmnshgtkiplqslki*pn**stklgiqlfaspvlkvfhtlqmlvms*eltpplk
lvfvyhhhskqlmhqly*kkfsklihhmvlklpskwinvlqvgihhkldqn*kkh*eklq
ifslakttyt*vkvvpshs*kcymtvsivnlsllvy*vqvqmlmvqmns*ifhlprny*v
vlfiy*msxcqvxlnk

Frame C:
FLFYFPIDKIKLNKMSLNIEKVKEFSNKAWDENILPTLSKYIEIPNQSPLYDAEWATNGY
TEEAIQLLEKWILAQGIKGISCEIKRIEGLTPIIFMVVEATKTEKVKTVLLYGHMDKQPP
LTDAWDEGLHPYKAVIKNNKLYGRGGADDGYSTFASISAIKALQEQSIPHDRYVIIIEGS
EESGSIHLPQYVEKFQAEIQI---

---*smsfr*cxwyrsifipyssssfrsc*ksinw*sr*kitr*nsttsfrsnrkmc*nf
rrnhl**ilmvqkyhssh*rfnpiddqqnlvsnylhhrc*rysthfkcw*chenshhr*s
*csfttiiqss*cincikrnfrn*stiwc*sylqng*mcfrlgfttn*trirksirrsfk
sflwqrprihe*rwfhpihenvt*pfqlsiyrywfirsrfkcswsk*ilrysicqeiikl
cylfirclxakxx*i

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO108 (AFO108Q) /CSM/AF/AFO1-A/AFO108Q.Seq.d/                       2494   0.0
AHL611 (AHL611Q) /CSM/AH/AHL6-A/AHL611Q.Seq.d/                       1441   0.0
AFH725 (AFH725Q) /CSM/AF/AFH7-B/AFH725Q.Seq.d/                       1439   0.0
AFO706 (AFO706Q) /CSM/AF/AFO7-A/AFO706Q.Seq.d/                       1431   0.0
AFL825 (AFL825Q) /CSM/AF/AFL8-B/AFL825Q.Seq.d/                       1431   0.0
VFC720 (VFC720Q) /CSM/VF/VFC7-A/VFC720Q.Seq.d/                       1423   0.0
AFC812 (AFC812Q) /CSM/AF/AFC8-A/AFC812Q.Seq.d/                       1423   0.0
VSD127 (VSD127Q) /CSM/VS/VSD1-B/VSD127Q.Seq.d/                       1417   0.0
AFM302 (AFM302Q) /CSM/AF/AFM3-A/AFM302Q.Seq.d/                       1415   0.0
CFJ404 (CFJ404Q) /CSM/CF/CFJ4-A/CFJ404Q.Seq.d/                       1411   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC137602|AC137602.12 Medicago truncatula clone mth2-14k4, complete sequence.    46  0.20  4
BI433279|BI433279.1 EST536040 P. infestans-challenged leaf Solanum tuberosum cDNA clone PPCBC62 5' sequence, mRNA sequence.    48  0.29  1
BI435717|BI435717.1 EST538478 P. infestans-challenged leaf Solanum tuberosum cDNA clone PPCCI86 5' sequence, mRNA sequence.    48  0.29  1
BQ046631|BQ046631.1 EST595749 P. infestans-challenged potato leaf, incompatible reaction Solanum tuberosum cDNA clone BPLI15C18 5' end, mRNA sequence.    48  0.29  1
BI435853|BI435853.1 EST538614 P. infestans-challenged leaf Solanum tuberosum cDNA clone PPCCL22 5' sequence, mRNA sequence.    48  0.29  1
BG591390|BG591390.1 EST499232 P. infestans-challenged leaf Solanum tuberosum cDNA clone BPLI8D17 5' sequence, mRNA sequence.    48  0.29  1
BI435340|BI435340.1 EST538101 P. infestans-challenged leaf Solanum tuberosum cDNA clone PPCCD08 5' sequence, mRNA sequence.    48  0.29  1
BQ112173|BQ112173.1 EST597749 mixed potato tissues Solanum tuberosum cDNA clone STMCF59 3' end, mRNA sequence.    48  0.29  1
CD821342|CD821342.1 BN25.041I21F011127 BN25 Brassica napus cDNA clone BN25041I21, mRNA sequence.    38  0.56  2
CC535979|CC535979.1 CH240_414D20.TARBAC13P2 CHORI-240 Bos taurus genomic clone CH240_414D20, genomic survey sequence.    36  0.65  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AE008923_2905(AE008923|pid:none) Xanthomonas axonopodis pv. citr...   239   e-107
CP000113_526(CP000113|pid:none) Myxococcus xanthus DK 1622, comp...   243   e-105
AM746676_157(AM746676|pid:none) Sorangium cellulosum 'So ce 56' ...   239   e-102
CP000267_395(CP000267|pid:none) Rhodoferax ferrireducens T118, c...   224   6e-94
CP001392_3415(CP001392|pid:none) Diaphorobacter sp. TPSY, comple...   223   1e-92
CP001013_323(CP001013|pid:none) Leptothrix cholodnii SP-6, compl...   220   2e-92
CP001053_1440(CP001053|pid:none) Burkholderia phytofirmans PsJN ...   215   3e-92
AM502249_240(AM502249|pid:none) Leishmania infantum chromosome 31.    192   6e-92
CT005268_204(CT005268|pid:none) Leishmania major strain Friedlin...   189   1e-90
CP000884_6020(CP000884|pid:none) Delftia acidovorans SPH-1, comp...   214   1e-90

 psg: 0.70  gvh: 0.29  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 44.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for AFO108 is cyt

>AFO108F.Seq
ATTTTTTATTTTATTTTCCAATTGATAAAATTAAATTAAATAAAATGTCATTAAATATTG
AAAAAGTTAAAGAATTTAGTAATAAAGCATGGGATGAAAATATTTTACCAACACTTTCAA
AATATATTGAAATTCCAAATCAATCACCATTATATGATGCAGAATGGGCAACCAATGGTT
ATACAGAAGAAGCAATTCAATTATTAGAGAAATGGATCTTAGCACAAGGTATCAAAGGTA
TTTCATGTGAAATTAAAAGAATTGAAGGATTAACACCAATTATTTTCATGGTAGTCGAAG
CAACCAAAACTGAAAAAGTTAAAACTGTACTTTTATATGGTCACATGGATAAACAACCAC
CACTCACTGATGCATGGGATGAAGGATTACATCCATACAAAGCAGTCATCAAGAATAACA
AACTCTATGGTCGTGGTGGTGCAGATGATGGTTATTCAACATTTGCTTCAATCTCTGCAA
TCAAAGCATTGCAAGAACAATCAATTCCACATGATCGTTATGTAATTATCATTGAAGGTT
CCGAAGAATCTGGTTCAATCCATTTACCACAATATGTTGAGAAGTTCCAAGCTGAAATTC
AAATTC----------

>AFO108Z.Seq
----------AATGAAGCATGTCATTCCGGTAGTGCNTCTGGTATCGTTCCATCTTCATT
CCGTATTCTTCGTCAAGTTTTAGATCGTGCTGAAAATCAATTAACTGGTGAAGTCGATAA
AAGATTACACGTTGAAATTCCACAACATCGTTTAGATCAAATCGCAAAATGTGCTGAAAT
TTTAGGCGAAACCATCTATGATGAATTCTCATGGTACAAAAATACCACTCCAGTCACTAA
AGATTTAACCCAATTGATGATCAACAAAACTTGGTATCCAACTATTTGCATCACCGGTGC
TGAAGGTATTCCACACACTTCAAATGCTGGTAATGTCATGAGAACTCACACCACCGTTAA
AGTTAGTGTTCGTTTACCACCATCATTCAAAGCAGCTGATGCATCAACTGTATTAAAAGA
AATTTTCGAAACTAATCCACCATATGGTGCTGAAGTTACCTTCAAAATGGATAAATGTGC
TTCAGGTTGGGATTCACCACAAACTAGACCAGAATTAGAAAAAGCATTAGGAGAAGCTTC
AAATCTTTTCTTTGGCAAAGACCACGTATACATGAGTGAAGGTGGTTCCATCCCATTCAT
GAAAATGTTACATGACCGTTTCAATTGTCAATTTATCGTTACTGGTTTATTAGGTCCAGG
TTCAAATGCTCATGGTCCAAATGAATTCTTAGATATTCCATTTGCCAAGAAATTATTAAG
TTGTGTTATTTATTTATTAGATGTCTCANTGCCAAGTTNNATTAAATAAA

>AFO108P.Seq
ATTTTTTATTTTATTTTCCAATTGATAAAATTAAATTAAATAAAATGTCATTAAATATTG
AAAAAGTTAAAGAATTTAGTAATAAAGCATGGGATGAAAATATTTTACCAACACTTTCAA
AATATATTGAAATTCCAAATCAATCACCATTATATGATGCAGAATGGGCAACCAATGGTT
ATACAGAAGAAGCAATTCAATTATTAGAGAAATGGATCTTAGCACAAGGTATCAAAGGTA
TTTCATGTGAAATTAAAAGAATTGAAGGATTAACACCAATTATTTTCATGGTAGTCGAAG
CAACCAAAACTGAAAAAGTTAAAACTGTACTTTTATATGGTCACATGGATAAACAACCAC
CACTCACTGATGCATGGGATGAAGGATTACATCCATACAAAGCAGTCATCAAGAATAACA
AACTCTATGGTCGTGGTGGTGCAGATGATGGTTATTCAACATTTGCTTCAATCTCTGCAA
TCAAAGCATTGCAAGAACAATCAATTCCACATGATCGTTATGTAATTATCATTGAAGGTT
CCGAAGAATCTGGTTCAATCCATTTACCACAATATGTTGAGAAGTTCCAAGCTGAAATTC
AAATTC----------AATGAAGCATGTCATTCCGGTAGTGCNTCTGGTATCGTTCCATC
TTCATTCCGTATTCTTCGTCAAGTTTTAGATCGTGCTGAAAATCAATTAACTGGTGAAGT
CGATAAAAGATTACACGTTGAAATTCCACAACATCGTTTAGATCAAATCGCAAAATGTGC
TGAAATTTTAGGCGAAACCATCTATGATGAATTCTCATGGTACAAAAATACCACTCCAGT
CACTAAAGATTTAACCCAATTGATGATCAACAAAACTTGGTATCCAACTATTTGCATCAC
CGGTGCTGAAGGTATTCCACACACTTCAAATGCTGGTAATGTCATGAGAACTCACACCAC
CGTTAAAGTTAGTGTTCGTTTACCACCATCATTCAAAGCAGCTGATGCATCAACTGTATT
AAAAGAAATTTTCGAAACTAATCCACCATATGGTGCTGAAGTTACCTTCAAAATGGATAA
ATGTGCTTCAGGTTGGGATTCACCACAAACTAGACCAGAATTAGAAAAAGCATTAGGAGA
AGCTTCAAATCTTTTCTTTGGCAAAGACCACGTATACATGAGTGAAGGTGGTTCCATCCC
ATTCATGAAAATGTTACATGACCGTTTCAATTGTCAATTTATCGTTACTGGTTTATTAGG
TCCAGGTTCAAATGCTCATGGTCCAAATGAATTCTTAGATATTCCATTTGCCAAGAAATT
ATTAAGTTGTGTTATTTATTTATTAGATGTCTCANTGCCAAGTTNNATTAAATAAA