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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFN894
Library AF
(Link to library)
Clone ID AFN894
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10734-1
Original site URL
Representative seq. ID AFN894P
(Link to Original site)
Representative DNA sequence 
>AFN894 (AFN894Q) /CSM/AF/AFN8-D/AFN894Q.Seq.d/
GCTTTGTTNGCTAATATTGCTTTTTCATGNGAAGGTCACCCAGAACATGATCATGGGGAT
GGGGATCATGANCATGATCATGATGAATCATTTGTTAAAATCTTANATTCTGATAATTTC
CATAATTCAGTTTNTGAACATGACGTAACTNTTGTTNTGTTTTATGCCCCATGGGGXXXX
XXXXXXCATTGACTCCACTCAAGATAACACTCAAGTTCTCGAAGATGTAAAGAAAATCGC
TACCTCACAAAAAGGTAATGCCGTTTTCTGTTGGGTCGATATGAAGAAATTCCCACAACA
AGCAACTCATATGGGTCTCTCTGGTAAAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGC
CAATAAAGCTCGTTACAATTTCGATGAAAAAGAAACCTTCTCATTCGATACCGTCTCCAA
ATGGATCCAAGATGTCATTGGTGGTAAAGTTTCTCCATTTGTTAAATCTCAACCAGAACC
AGAATCAAATGATGCTCCAGTTAAGGTTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTT
AGACTCACCAAAGGATGTTTTGGTTGAATTCTACGCTCCATGGTGCGGTCATTGCAAGAA
TCTTGCTCCAATCTACGATAAATTAGGTGAATACTTAAAAGACGTTGAATCTGTCTCAAT
CGTAAAGATTGATGCCGACTCAAATGATGTACCAAGTGATATCGAAATTAGAGGTTACCC
AACCATCATGCTCTTCAAAGCTGATGACAAAGAAAATCCAATCTCTTATGAAGGTCAAAG
AAATGATCATATGAACTTTGTTGAATTCATTCAAGATAACGCTGCCATTGAATTCAAATT
ACCATCATCTCAAACTGANATAATGTGAATCTAAAAAAGATTCTTCTGCTAAACANANAA
TATAATAATTAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
ALXANIAFSXEGHPEHDHGDGDHXHDHDESFVKILXSDNFHNSVXEHDVTXVXFYAPW--
-

---IDSTQDNTQVLEDVKKIATSQKGNAVFCWVDMKKFPQQATHMGLSGKVVPAISVDSV
ANKARYNFDEKETFSFDTVSKWIQDVIGGKVSPFVKSQPEPESNDAPVKVAVGTTFKKLV
LDSPKDVLVEFYAPWCGHCKNLAPIYDKLGEYLKDVESVSIVKIDADSNDVPSDIEIRGY
PTIMLFKADDKENPISYEGQRNDHMNFVEFIQDNAAIEFKLPSSQTXIM*i*krffc*tx
niii
Translated Amino Acid sequence (All Frames) 
Frame A:
ALXANIAFSXEGHPEHDHGDGDHXHDHDESFVKILXSDNFHNSVXEHDVTXVXFYAPW--
-

---h*lhsr*hsssrrckenryltkr*crfllgryeeipttsnsygslw*scsshlc*fs
cq*sslqfr*krnlliryrlqmdprchww*sfsic*istrtrik*css*gcrwyhfqkvg
frltkgcfg*ilrsmvrslqescsnlr*ir*ilkrr*iclnrkd*crlk*ctk*yrn*rl
pnhhalqs**qrksnll*rskk*syelc*ihsr*rch*iqitiisn*xnvnlkkilllnx
xynn*

Frame B:
lcxlillfhxkvtqnmimgmgimxmimmnhllksxiliisiiqfxnmt*llxcfmphg--
-

---IDSTQDNTQVLEDVKKIATSQKGNAVFCWVDMKKFPQQATHMGLSGKVVPAISVDSV
ANKARYNFDEKETFSFDTVSKWIQDVIGGKVSPFVKSQPEPESNDAPVKVAVGTTFKKLV
LDSPKDVLVEFYAPWCGHCKNLAPIYDKLGEYLKDVESVSIVKIDADSNDVPSDIEIRGY
PTIMLFKADDKENPISYEGQRNDHMNFVEFIQDNAAIEFKLPSSQTXIM*i*krffc*tx
niii

Frame C:
fvx*ycffmxrsprt*swgwgs*x*s**iic*nlxf**fp*fsx*t*rnxcxvlcpmg--
-

---ltplkitlkfskm*rkslphkkvmpfsvgsi*rnshnkqliwvslvklfqpslliql
piklvtismkkkpshsipspngskmslvvkflhllnlnqnqnqmmlqlrlplvplskswf
*thqrmfwlnstlhgaviarillqstin*vnt*ktlnlsqs*rlmptqmmyqvisklevt
qpscssklmtkkiqslmkvkemii*tllnsfkitlplnsnyhhlklx*ceskkdssakxx
i**l
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFN894 (AFN894Q) /CSM/AF/AFN8-D/AFN894Q.Seq.d/                       1689   0.0
AFN855 (AFN855Q) /CSM/AF/AFN8-C/AFN855Q.Seq.d/                       1386   0.0
AFM464 (AFM464Q) /CSM/AF/AFM4-C/AFM464Q.Seq.d/                       1386   0.0
AFK217 (AFK217Q) /CSM/AF/AFK2-A/AFK217Q.Seq.d/                       1386   0.0
AFO368 (AFO368Q) /CSM/AF/AFO3-C/AFO368Q.Seq.d/                       1380   0.0
AFN679 (AFN679Q) /CSM/AF/AFN6-D/AFN679Q.Seq.d/                       1380   0.0
AFN461 (AFN461Q) /CSM/AF/AFN4-C/AFN461Q.Seq.d/                       1380   0.0
AFL563 (AFL563Q) /CSM/AF/AFL5-C/AFL563Q.Seq.d/                       1380   0.0
AFO479 (AFO479Q) /CSM/AF/AFO4-D/AFO479Q.Seq.d/                       1378   0.0
SFI119 (SFI119Q) /CSM/SF/SFI1-A/SFI119Q.Seq.d/                       1372   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BJ171852|BJ171852.1 Physcomitrella patens subsp. patens cDNA clone:pph30f12, 3' end, single read.   599  e-167  1
BM398179|BM398179.1 5009-0-41-G11.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    74  3e-09  1
AL924030|AL924030.1 Danio rerio EST sequence, clone 134-B07-2.    70  5e-08  1
CB176061|CB176061.1 pl17e07.y3 Ancylostoma ceylanicum adult Ancylostoma ceylanicum cDNA 5' similar to TR:O17908 O17908 H06O01.1 PROTEIN. [1] ;, mRNA sequence.    70  5e-08  1
BM280519|BM280519.1 ki01h03.y1 Ascaris suum L4 pSPORT1 Zarlenga v1 Ascaris suum cDNA 5' similar to SW:ERP5_CAEEL Q11067 PROBABLE PROTEIN DISULFIDE ISOMERASE P5 PRECURSOR ;, mRNA sequence.    68  2e-07  1
AW255071|AW255071.1 ML1365 peppermint glandular trichome Mentha x piperita cDNA, mRNA sequence.    64  3e-06  1
BM315730|BM315730.1 fw64d09.y1 Sugano SJD adult male Danio rerio cDNA clone 5612584 5' similar to SW:ER60_HUMAN P30101 PROBABLE PROTEIN DISULFIDE ISOMERASE ER-60 PRECURSOR ;, mRNA sequence.    62  1e-05  1
BM308748|BM308748.1 sak49h10.y1 Gm-c1036 Glycine max cDNA clone SOYBEAN CLONE ID: Gm-c1036-6523 5' similar to TR:P93358 P93358 PROTEIN DISULFIDE-ISOMERASE PRECURSOR. ;, mRNA sequence.    60  5e-05  1
BU795021|S34275 protein disulfide-isomerase homolog precursor - fluke  (Schistosoma mansoni), mRNA sequence.    60  5e-05  1
BM174369|PDA4_MOUSE PROTEIN DISULFIDE ISOMERASE A4 PRECURSOR (PROTEIN ERP-72) (ERP72), mRNA sequence.    60  5e-05  1
dna update 2003. 9.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q12730) RecName: Full=Protein disulfide-isomerase;          Sho...   174   3e-42
EU016183_1(EU016183|pid:none) Haemaphysalis longicornis strain G...   173   8e-42
(Q00248) RecName: Full=Protein disulfide-isomerase;          Sho...   172   2e-41
DQ066336_1(DQ066336|pid:none) Ixodes scapularis isolate IS-6-12-...   168   3e-40
CU633901_146(CU633901|pid:none) Podospora anserina genomic DNA c...   166   1e-39
DQ377176_1(DQ377176|pid:none) Amblyomma variegatum protein disul...   163   8e-39
AE013599_1413(AE013599|pid:none) Drosophila melanogaster chromos...   154   3e-36
CR382131_125(CR382131|pid:none) Yarrowia lipolytica strain CLIB1...   152   1e-35
AB022013_1(AB022013|pid:none) Antheraea pernyi mRNA for protein ...   151   2e-35
AK290971_1(AK290971|pid:none) Homo sapiens cDNA FLJ78033 complet...   150   4e-35
protein update 2009. 4.25
PSORT 

 psg: 0.75  gvh: 0.52  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.16  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 20.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system

>> prediction for AFN894 is cyt
5' end seq. ID AFN894F
5' end seq. 
>AFN894F.Seq
GCTTTGTTNGCTAATATTGCTTTTTCATGNGAAGGTCACCCAGAACATGATCATGGGGAT
GGGGATCATGANCATGATCATGATGAATCATTTGTTAAAATCTTANATTCTGATAATTTC
CATAATTCAGTTTNTGAACATGACGTAACTNTTGTTNTGTTTTATGCCCCATGGGG----
------
Length of 5' end seq. 176
3' end seq. ID AFN894Z
3' end seq. 
>AFN894Z.Seq
----------CATTGACTCCACTCAAGATAACACTCAAGTTCTCGAAGATGTAAAGAAAA
TCGCTACCTCACAAAAAGGTAATGCCGTTTTCTGTTGGGTCGATATGAAGAAATTCCCAC
AACAAGCAACTCATATGGGTCTCTCTGGTAAAGTTGTTCCAGCCATCTCTGTTGATTCAG
TTGCCAATAAAGCTCGTTACAATTTCGATGAAAAAGAAACCTTCTCATTCGATACCGTCT
CCAAATGGATCCAAGATGTCATTGGTGGTAAAGTTTCTCCATTTGTTAAATCTCAACCAG
AACCAGAATCAAATGATGCTCCAGTTAAGGTTGCCGTTGGTACCACTTTCAAAAAGTTGG
TTTTAGACTCACCAAAGGATGTTTTGGTTGAATTCTACGCTCCATGGTGCGGTCATTGCA
AGAATCTTGCTCCAATCTACGATAAATTAGGTGAATACTTAAAAGACGTTGAATCTGTCT
CAATCGTAAAGATTGATGCCGACTCAAATGATGTACCAAGTGATATCGAAATTAGAGGTT
ACCCAACCATCATGCTCTTCAAAGCTGATGACAAAGAAAATCCAATCTCTTATGAAGGTC
AAAGAAATGATCATATGAACTTTGTTGAATTCATTCAAGATAACGCTGCCATTGAATTCA
AATTACCATCATCTCAAACTGANATAATGTGAATCTAAAAAAGATTCTTCTGCTAAACAN
ANAATATAATAATTAA
Length of 3' end seq. 726
Connected seq. ID AFN894P
Connected seq. 
>AFN894P.Seq
GCTTTGTTNGCTAATATTGCTTTTTCATGNGAAGGTCACCCAGAACATGATCATGGGGAT
GGGGATCATGANCATGATCATGATGAATCATTTGTTAAAATCTTANATTCTGATAATTTC
CATAATTCAGTTTNTGAACATGACGTAACTNTTGTTNTGTTTTATGCCCCATGGGG----
------CATTGACTCCACTCAAGATAACACTCAAGTTCTCGAAGATGTAAAGAAAATCGC
TACCTCACAAAAAGGTAATGCCGTTTTCTGTTGGGTCGATATGAAGAAATTCCCACAACA
AGCAACTCATATGGGTCTCTCTGGTAAAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGC
CAATAAAGCTCGTTACAATTTCGATGAAAAAGAAACCTTCTCATTCGATACCGTCTCCAA
ATGGATCCAAGATGTCATTGGTGGTAAAGTTTCTCCATTTGTTAAATCTCAACCAGAACC
AGAATCAAATGATGCTCCAGTTAAGGTTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTT
AGACTCACCAAAGGATGTTTTGGTTGAATTCTACGCTCCATGGTGCGGTCATTGCAAGAA
TCTTGCTCCAATCTACGATAAATTAGGTGAATACTTAAAAGACGTTGAATCTGTCTCAAT
CGTAAAGATTGATGCCGACTCAAATGATGTACCAAGTGATATCGAAATTAGAGGTTACCC
AACCATCATGCTCTTCAAAGCTGATGACAAAGAAAATCCAATCTCTTATGAAGGTCAAAG
AAATGATCATATGAACTTTGTTGAATTCATTCAAGATAACGCTGCCATTGAATTCAAATT
ACCATCATCTCAAACTGANATAATGTGAATCTAAAAAAGATTCTTCTGCTAAACANANAA
TATAATAATTAA
Length of connected seq. 902
Full length Seq ID -
Full length Seq. -
Length of full length seq. -