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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHG547
Library VH
(Link to library)
Clone ID VHG547
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15761-1
Original site URL
Representative seq. ID VHG547P
(Link to Original site)
Representative DNA sequence 
>VHG547 (VHG547Q) /CSM/VH/VHG5-B/VHG547Q.Seq.d/
CAACAGCAACAAACTATAAATGGTAGTGCACAAAAAGTTGTGAAATCAATTAGTTCAACT
ACACCCATTTCAAAATTATTTGGTGCATTTGGACCTTTTGGTAGTAGTAGTAGTAATACA
CCATCATCGACATCAGCAACTACAGCAGCAAATGGTAAAAATACACCAATGCCAAGTAAT
AGTGGTATAGCTTCATTAAGTGCAGGTGGATCAACGATTATAGCAGGTTTATCAGGTTCA
CAATCTTTAAACAATTTACAATCTGCACAATTATCAGGTGCTTTAAATACTCAACATTAT
ATTCGTACACCTTCTTTAAATTTAACAAGTGATCTCTCTGAAAGATTAACTGAAAAACAA
GATAGAGAAAGTTTAGATTTTTTAGTTGGTGATTTATTATTTAGTTCAGCTCAAAGATTA
GAAGAATTGGCAATAATTATTGGATATTTACCAGTTGATGATTATAATAGTGAAATGTTC
TTTCAAAATGTAGAAGAAGTTATATTTAAGAGTGTAGAGAATAAAAAGATTGAAGTTGAT
TCAATGACAGCATTTAGTTATCAGCCATTACAAGTCTCACTTCAATCATCGAAACAATTC
ACTCAACTXXXXXXXXXXATCGAGCTTCCAACTTATCTTTATCAAATAGATCTATCAATT
CAAAGTAGGGCTTATGTTGGTACAATAGTTCTTTTTGAAATTGAAATTACAAATTTAAAA
CAACAACAACAACAACAAAAAGAAAGTAATAATGATAACGGTAATGAAAAACAACAAAAA
CAACAACAATTACAATATCATATAGTTGNTGATTCACAAATTTGGATGATATCTGGTAAG
AGTAAACATACATTTTCATTTAATAGTGATACGGTTGGTGAAAAGCTTAAATTTAGTTGT
GGTTTAATTCCAATCTCCTCTGGCTCATTACCAATTCCAAAAGTAACTTTAGTCGGTATA
AATAATTCAAATATTTCTTATCCAAAAACTAAAAATGAAAAGATATTCGTTTATCCATCA
CCACAAATTTATTCATGTCATCAATTACAAGATAATA
sequence update 2002.10.25
Translated Amino Acid sequence 
QQQQTINGSAQKVVKSISSTTPISKLFGAFGPFGSSSSNTPSSTSATTAANGKNTPMPSN
SGIASLSAGGSTIIAGLSGSQSLNNLQSAQLSGALNTQHYIRTPSLNLTSDLSERLTEKQ
DRESLDFLVGDLLFSSAQRLEELAIIIGYLPVDDYNSEMFFQNVEEVIFKSVENKKIEVD
SMTAFSYQPLQVSLQSSKQFTQ---

---IELPTYLYQIDLSIQSRAYVGTIVLFEIEITNLKQQQQQQKESNNDNGNEKQQKQQQ
LQYHIVXDSQIWMISGKSKHTFSFNSDTVGEKLKFSCGLIPISSGSLPIPKVTLVGINNS
NISYPKTKNEKIFVYPSPQIYSCHQLQDN
Translated Amino Acid sequence (All Frames) 
Frame A:
QQQQTINGSAQKVVKSISSTTPISKLFGAFGPFGSSSSNTPSSTSATTAANGKNTPMPSN
SGIASLSAGGSTIIAGLSGSQSLNNLQSAQLSGALNTQHYIRTPSLNLTSDLSERLTEKQ
DRESLDFLVGDLLFSSAQRLEELAIIIGYLPVDDYNSEMFFQNVEEVIFKSVENKKIEVD
SMTAFSYQPLQVSLQSSKQFTQ---

---IELPTYLYQIDLSIQSRAYVGTIVLFEIEITNLKQQQQQQKESNNDNGNEKQQKQQQ
LQYHIVXDSQIWMISGKSKHTFSFNSDTVGEKLKFSCGLIPISSGSLPIPKVTLVGINNS
NISYPKTKNEKIFVYPSPQIYSCHQLQDN

Frame B:
nsnkl*mvvhkkl*nqlvqlhpfqnylvhldllvvvvvihhhrhqqlqqqmvkihqcqvi
vv*lh*vqvdqrl*qvyqvhnl*tiynlhnyqvl*ilniifvhll*i*qvislkd*lknk
iekv*if*lviyylvqlkd*knwq*lldiyqlmiiivkcsfkm*kklylrv*rikrlkli
q*qhlvishykshfnhrnnsln---

---ssfqlifik*iyqfkvglmlvq*fflklklqi*nnnnnnkkkvimitvmknnknnnn
ynii*lxihkfg*ylvrvnihfhlivirlvkslnlvvv*fqsplahyqfqk*l*sv*iiq
ifliqklkmkrysfihhhkfihvinykii

Frame C:
tatnykw*ctkscein*fnythfkiiwciwtfw*****ytiidisnysskw*kytnak**
wysfikcrwindysrfirftifkqftictiircfkystlysytffkfnk*sl*kin*ktr
*rkfrffsw*fii*fsskirrignnywifts**l***nvlskcrrsyi*ecre*kd*s*f
ndsi*lsaitsltsiietihst---

---rasnlslsnrsinsk*glcwynssf*n*nykfkttttttkrk****r**kttkttti
tisysx*ftnlddiw*e*tyifi***ygw*ka*i*lwfnsnllwlitnsksnfsryk*fk
yflskn*k*kdirlsittnlfmssitr*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHG547 (VHG547Q) /CSM/VH/VHG5-B/VHG547Q.Seq.d/                       1364   0.0
AHG346 (AHG346Q) /CSM/AH/AHG3-B/AHG346Q.Seq.d/                       1205   0.0
AHL183 (AHL183Q) /CSM/AH/AHL1-D/AHL183Q.Seq.d/                       1191   0.0
AHG114 (AHG114Q) /CSM/AH/AHG1-A/AHG114Q.Seq.d/                        977   0.0
AFF234 (AFF234Q) /CSM/AF/AFF2-B/AFF234Q.Seq.d/                        517   e-145
AFE423 (AFE423Q) /CSM/AF/AFE4-A/AFE423Q.Seq.d/                        517   e-145
AHH138 (AHH138Q) /CSM/AH/AHH1-B/AHH138Q.Seq.d/                        511   e-144
CHH616 (CHH616Q) /CSM/CH/CHH6-A/CHH616Q.Seq.d/                        381   e-104
CHA111 (CHA111Q) /CSM/CH/CHA1-A/CHA111Q.Seq.d/                        289   4e-77
AHI675 (AHI675Q) /CSM/AH/AHI6-D/AHI675Q.Seq.d/                        151   3e-35
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ421971) Dictyostelium discoideum cDNA clone:ddv44m11, 5' ...  1205   0.0    1
(BJ333628) Dictyostelium discoideum cDNA clone:dda43l11, 5' ...  1205   0.0    1
(BJ336402) Dictyostelium discoideum cDNA clone:dda53e21, 5' ...  1191   0.0    1
(AC116984) Dictyostelium discoideum chromosome 2 map 2567470...   827   0.0    4
(BJ333546) Dictyostelium discoideum cDNA clone:dda43k03, 5' ...   977   0.0    1
(BJ440681) Dictyostelium discoideum cDNA clone:ddv44m11, 3' ...   513   0.0    2
(BJ351422) Dictyostelium discoideum cDNA clone:dda43l11, 3' ...   505   0.0    2
(BJ342026) Dictyostelium discoideum cDNA clone:dda9n06, 3' e...   505   0.0    2
(BJ340005) Dictyostelium discoideum cDNA clone:dda11c10, 3' ...   505   0.0    2
(BJ352022) Dictyostelium discoideum cDNA clone:dda45k09, 3' ...   500   0.0    2
dna update 2008.11. 3
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q556Z3) RecName: Full=Trafficking protein particle complex subu...   285   2e-75
AP004047_2(AP004047|pid:none) Oryza sativa Japonica Group genomi...    40   0.18
CR382123_304(CR382123|pid:none) Kluyveromyces lactis strain NRRL...    36   2.6
(Q9VFB7) RecName: Full=Trafficking protein particle complex subu...    35   5.8
protein update 2009. 3.10
PSORT 

 psg: 0.70  gvh: 0.50  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.54  mip: 0.17
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 80.0 %: mitochondrial
	 12.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: cytoplasmic

>> prediction for VHG547 is mit
5' end seq. ID VHG547F
5' end seq. 
>VHG547F.Seq
CAACAGCAACAAACTATAAATGGTAGTGCACAAAAAGTTGTGAAATCAATTAGTTCAACT
ACACCCATTTCAAAATTATTTGGTGCATTTGGACCTTTTGGTAGTAGTAGTAGTAATACA
CCATCATCGACATCAGCAACTACAGCAGCAAATGGTAAAAATACACCAATGCCAAGTAAT
AGTGGTATAGCTTCATTAAGTGCAGGTGGATCAACGATTATAGCAGGTTTATCAGGTTCA
CAATCTTTAAACAATTTACAATCTGCACAATTATCAGGTGCTTTAAATACTCAACATTAT
ATTCGTACACCTTCTTTAAATTTAACAAGTGATCTCTCTGAAAGATTAACTGAAAAACAA
GATAGAGAAAGTTTAGATTTTTTAGTTGGTGATTTATTATTTAGTTCAGCTCAAAGATTA
GAAGAATTGGCAATAATTATTGGATATTTACCAGTTGATGATTATAATAGTGAAATGTTC
TTTCAAAATGTAGAAGAAGTTATATTTAAGAGTGTAGAGAATAAAAAGATTGAAGTTGAT
TCAATGACAGCATTTAGTTATCAGCCATTACAAGTCTCACTTCAATCATCGAAACAATTC
ACTCAACTNNNNNNNNNN
Length of 5' end seq. 618
3' end seq. ID VHG547Z
3' end seq. 
>VHG547Z.Seq
NNNNNNNNNNATCGAGCTTCCAACTTATCTTTATCAAATAGATCTATCAATTCAAAGTAG
GGCTTATGTTGGTACAATAGTTCTTTTTGAAATTGAAATTACAAATTTAAAACAACAACA
ACAACAACAAAAAGAAAGTAATAATGATAACGGTAATGAAAAACAACAAAAACAACAACA
ATTACAATATCATATAGTTGNTGATTCACAAATTTGGATGATATCTGGTAAGAGTAAACA
TACATTTTCATTTAATAGTGATACGGTTGGTGAAAAGCTTAAATTTAGTTGTGGTTTAAT
TCCAATCTCCTCTGGCTCATTACCAATTCCAAAAGTAACTTTAGTCGGTATAAATAATTC
AAATATTTCTTATCCAAAAACTAAAAATGAAAAGATATTCGTTTATCCATCACCACAAAT
TTATTCATGTCATCAATTACAAGATAATA
Length of 3' end seq. 449
Connected seq. ID VHG547P
Connected seq. 
>VHG547P.Seq
CAACAGCAACAAACTATAAATGGTAGTGCACAAAAAGTTGTGAAATCAATTAGTTCAACT
ACACCCATTTCAAAATTATTTGGTGCATTTGGACCTTTTGGTAGTAGTAGTAGTAATACA
CCATCATCGACATCAGCAACTACAGCAGCAAATGGTAAAAATACACCAATGCCAAGTAAT
AGTGGTATAGCTTCATTAAGTGCAGGTGGATCAACGATTATAGCAGGTTTATCAGGTTCA
CAATCTTTAAACAATTTACAATCTGCACAATTATCAGGTGCTTTAAATACTCAACATTAT
ATTCGTACACCTTCTTTAAATTTAACAAGTGATCTCTCTGAAAGATTAACTGAAAAACAA
GATAGAGAAAGTTTAGATTTTTTAGTTGGTGATTTATTATTTAGTTCAGCTCAAAGATTA
GAAGAATTGGCAATAATTATTGGATATTTACCAGTTGATGATTATAATAGTGAAATGTTC
TTTCAAAATGTAGAAGAAGTTATATTTAAGAGTGTAGAGAATAAAAAGATTGAAGTTGAT
TCAATGACAGCATTTAGTTATCAGCCATTACAAGTCTCACTTCAATCATCGAAACAATTC
ACTCAACT----------ATCGAGCTTCCAACTTATCTTTATCAAATAGATCTATCAATT
CAAAGTAGGGCTTATGTTGGTACAATAGTTCTTTTTGAAATTGAAATTACAAATTTAAAA
CAACAACAACAACAACAAAAAGAAAGTAATAATGATAACGGTAATGAAAAACAACAAAAA
CAACAACAATTACAATATCATATAGTTGNTGATTCACAAATTTGGATGATATCTGGTAAG
AGTAAACATACATTTTCATTTAATAGTGATACGGTTGGTGAAAAGCTTAAATTTAGTTGT
GGTTTAATTCCAATCTCCTCTGGCTCATTACCAATTCCAAAAGTAACTTTAGTCGGTATA
AATAATTCAAATATTTCTTATCCAAAAACTAAAAATGAAAAGATATTCGTTTATCCATCA
CCACAAATTTATTCATGTCATCAATTACAAGATAATA
Length of connected seq. 1047
Full length Seq ID -
Full length Seq. -
Length of full length seq. -