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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHG502
Library VH
(Link to library)
Clone ID VHG502
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12609-1
Original site URL
Representative seq. ID
(Link to Original site)
Representative DNA sequence 
>VHG502 (VHG502Q) /CSM/VH/VHG5-A/VHG502Q.Seq.d/
CAAGGGTCCAGTGGGTATCAAAGCCACTGTACCCTACATTAAACAAGATGTACCCGTTAT
CATTGTCTTTAGAGCATTGGGTTTTGTTGCCGATAAAGATATTTTAGAACATATTTGTTA
TGACTTCAAGGATGCCCAAATGATGGATCTTATGAGACCATCACTCGAGGAGTCCTTTGT
CATTCAATCACAAGAGGTTGCTTTGGATTATTTGGGTAAACGTGGTTCCACAATGGGTAC
TCGTGAACAACGTATCAAATTCGCTCGTGAAGTATTACAAAAGGAAATGTTACCACATTG
TTAGTGTCGCTGAATTCTATGAAACAAAGAAAGCATTTTACTTTGGTTATATCACTCATC
GTCTTTTATTGGCTTCACTTGAAAGACGTCCATTGGATGATCGTGATCATTTTGCAAATA
AACGTTTGGATTTGGCTGGTCCACTACTTGGTACACTTTTCCGTCAACTTTTTAAAAAAC
TTACAAAAGATGTCCGTCTTTATCTTCAACGTTGTATAGATAAAGATAAAGAATTCATTG
CATCATCTGCTGTTAAATCCAAAACAATAACAAGTGGTTTGAAATATTCATTGGCAACTG
GTAXXXXXXXXXX
sequence update 2002.10.25
Translated Amino Acid sequence 
rvqwvskplyptlnkmyplslslehwvllpikif*nifvmtsrmpk*wil*DHHSRSPLS
FNHKRLLWIIWVNVVPQWVLVNNVSNSLVKYYKRKCYHIVSVAEFYETKKAFYFGYITHR
LLLASLERRPLDDRDHFANKRLDLAGPLLGTLFRQLFKKLTKDVRLYLQRCIDKDKEFIA
SSAVKSKTITSGLKYSLATG---
Translated Amino Acid sequence (All Frames) 
Frame A:
qgssgyqshctlh*trctryhcl*sigfccr*ryfrtyll*lqgcpndgsyetitrgvlc
hsitrgcfglfg*twfhngys*ttyqirs*sitkgnvttllvslnsmkqrkhftlvisli
vfywlhlkdvhwmiviilqinvwiwlvhylvhfsvnflknlqkmsvfifnvv*ikiknsl
hhlllnpkq*qvv*nihwql---


Frame B:
kgpvgikatvpyikqdvpviivfralgfvadkdilehicydfkdaqmmdlmrpsleesfv
iqsqevaldylgkrgstmgtreqrikfarevlqkemlphc*cr*il*nkesillwlyhss
sfigft*ktsig*s*sfck*tfgfgwsttwytfpstf*ktykrcpslsstlyr*r*rihc
iicc*iqnnnkwfeifignw---


Frame C:
rvqwvskplyptlnkmyplslslehwvllpikif*nifvmtsrmpk*wil*DHHSRSPLS
FNHKRLLWIIWVNVVPQWVLVNNVSNSLVKYYKRKCYHIVSVAEFYETKKAFYFGYITHR
LLLASLERRPLDDRDHFANKRLDLAGPLLGTLFRQLFKKLTKDVRLYLQRCIDKDKEFIA
SSAVKSKTITSGLKYSLATG---
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHG502 (VHG502Q) /CSM/VH/VHG5-A/VHG502Q.Seq.d/                       1195   0.0
CHK802 (CHK802Q) /CSM/CH/CHK8-A/CHK802Q.Seq.d/                        952   0.0
AHM462 (AHM462Q) /CSM/AH/AHM4-C/AHM462Q.Seq.d/                        948   0.0
AHN418 (AHN418Q) /CSM/AH/AHN4-A/AHN418Q.Seq.d/                        383   e-105
AHF591 (AHF591Q) /CSM/AH/AHF5-D/AHF591Q.Seq.d/                        379   e-104
SHK610 (SHK610Q) /CSM/SH/SHK6-A/SHK610Q.Seq.d/                        333   2e-90
AFI586 (AFI586Q) /CSM/AF/AFI5-D/AFI586Q.Seq.d/                        168   6e-41
VFN513 (VFN513Q) /CSM/VF/VFN5-A/VFN513Q.Seq.d/                         38   0.15
VFL857 (VFL857Q) /CSM/VF/VFL8-C/VFL857Q.Seq.d/                         38   0.15
VFI782 (VFI782Q) /CSM/VF/VFI7-D/VFI782Q.Seq.d/                         38   0.15
own update 2002.12.28
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ421839) Dictyostelium discoideum cDNA clone:ddv44c01, 5' ...  1195   0.0    1
(BJ367521) Dictyostelium discoideum cDNA clone:ddc43d02, 5' ...   605   0.0    2
(BJ336926) Dictyostelium discoideum cDNA clone:dda55l16, 5' ...   605   0.0    3
(BJ337324) Dictyostelium discoideum cDNA clone:dda57d06, 5' ...   383   e-102  1
(BJ333450) Dictyostelium discoideum cDNA clone:dda42e23, 5' ...   379   e-101  1
(BJ397090) Dictyostelium discoideum cDNA clone:dds45c04, 5' ...   333   4e-87  1
(BJ327028) Dictyostelium discoideum cDNA clone:dda18k21, 5' ...   168   1e-37  1
(DQ017114) Homalocladium platycladum RNA polymerase II secon...    82   1e-11  2
(CJ392804) Molgula tectiformis cDNA, gonad clone:mtgd007j06,...    52   4e-10  3
(EI497883) PV_GBa0116K18.f PV_GBa Phaseolus vulgaris genomic...    78   4e-10  1
dna update 2008.11. 3
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54J75) RecName: Full=DNA-directed RNA polymerase II subunit rp...   201   e-103
DQ058635_1(DQ058635|pid:none) Spinacia oleracea RNA polymerase I...   160   2e-76
DQ058632_1(DQ058632|pid:none) Platanus orientalis RNA polymerase...   158   4e-76
DQ058630_1(DQ058630|pid:none) Eschscholzia californica RNA polym...   159   5e-76
DQ017133_1(DQ017133|pid:none) Vitis piasezkii RNA polymerase II ...   159   5e-76
DQ020634_1(DQ020634|pid:none) Berberidopsis beckleri RNA polymer...   159   5e-76
DQ020633_1(DQ020633|pid:none) Berberidopsis corallina RNA polyme...   159   5e-76
DQ017121_1(DQ017121|pid:none) Nepenthes maxima RNA polymerase II...   158   5e-76
DQ017113_1(DQ017113|pid:none) Hibbertia scandens RNA polymerase ...   158   7e-76
DQ017100_1(DQ017100|pid:none) Dicentra formosa RNA polymerase II...   159   1e-75
protein update 2009. 7.12
PSORT 

 psg: 0.66  gvh: 0.51  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.42  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: mitochondrial
	 20.0 %: cytoplasmic
	 20.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane

>> prediction for VHG502 is mit
5' end seq. ID VHG502F
5' end seq. 
>VHG502F.Seq
CAAGGGTCCAGTGGGTATCAAAGCCACTGTACCCTACATTAAACAAGATGTACCCGTTAT
CATTGTCTTTAGAGCATTGGGTTTTGTTGCCGATAAAGATATTTTAGAACATATTTGTTA
TGACTTCAAGGATGCCCAAATGATGGATCTTATGAGACCATCACTCGAGGAGTCCTTTGT
CATTCAATCACAAGAGGTTGCTTTGGATTATTTGGGTAAACGTGGTTCCACAATGGGTAC
TCGTGAACAACGTATCAAATTCGCTCGTGAAGTATTACAAAAGGAAATGTTACCACATTG
TTAGTGTCGCTGAATTCTATGAAACAAAGAAAGCATTTTACTTTGGTTATATCACTCATC
GTCTTTTATTGGCTTCACTTGAAAGACGTCCATTGGATGATCGTGATCATTTTGCAAATA
AACGTTTGGATTTGGCTGGTCCACTACTTGGTACACTTTTCCGTCAACTTTTTAAAAAAC
TTACAAAAGATGTCCGTCTTTATCTTCAACGTTGTATAGATAAAGATAAAGAATTCATTG
CATCATCTGCTGTTAAATCCAAAACAATAACAAGTGGTTTGAAATATTCATTGGCAACTG
GTANNNNNNNNNN
Length of 5' end seq. 613
3' end seq. ID -
3' end seq. -
Length of 3' end seq. -
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -