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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFB648
Library AF
(Link to library)
Clone ID AFB648
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFB648P
(Link to Original site)
Representative DNA sequence 
>AFB648 (AFB648Q) /CSM/AF/AFB6-B/AFB648Q.Seq.d/
ATTTTCCATTGATAAAATTAATTAAATAAAATGTCATTAAATATTGAAAAAGTTAAAGAA
TTTAGTAATAAAGCATGGGATGAAAATATTTTACCAACACTTTCAAAATATATTGAAATT
CCAAATCAATCACCATTATATGATGCAGAATGGGCAACCAATGGTTATACAGAAGAAGCA
ATTCAATTATTAGAGAAATGGATCTTAGCACAAGGTATCAAAGGTATTTCATGTGAAATT
AAAAGAATTGAAGGATTAACACCAATTATTTTCATGGTAGTCGAAGCAACCAAAACTGAA
AAAGTTAAAACTGTACTTTTATATGGTCACATGGATAAACAACCACCACTCACTGATGCA
TGGGATGAAGGATTACATCCATACAAAGCAGTCATCAAGAATAACAAACTCTATGGTCGT
GGTGGTGCAGATGATGGTTATTCAACATTTGCTTCAATCTCTGCAATCAAAGCATTGCAA
GAACAATCAATTCCACATGATCGTTATGTAATTATCATTGAAGGTTCCGAAGAATCTGGT
TCAATCCATTTACCACAATATGTTGAGAAGTTCCAAGCTGAAATTCAAATTCCATCATTG
GTAGTTXXXXXXXXXXGTCAAGTTTTAGATCGTGCTGAAAATCAATTAACTGGTGAAGTC
GATAAAAGATTACACGTTGAAATTCCACAACATCGTTTAGATCAAATCGCAAAATGTGCT
GAAATTTTAGGCGAAACCATCTATGATGAATTCTCATGGTACAAAAATACCACTCCAGTC
ACTAAAGATTTAACCCAATTGATGATCAACAAAACTTGGTATCCAACTATTTGCATCACC
GGTGCTGAAGGTATTCCACACACTTCAAATGCTGGTAATGTCATGAGAACTCACACCACC
GTTAAAGTTAGTGTTCGTTTACCACCATCATTCAAAGCAGCTGATGCATCAACTGTATTA
AAAGAAATTTTCGAAACTAATCCACCATATGGTGCTGAAGTTACCTTCAAAATGGATAAA
TGTGCTTCAGGTTGGGATTCACCACAAACTAGACCAGAATTAGAAAAAGCATTAGGAGAA
GCTTCAAATCTTTTCTTTGGCAAAGACCACGTATACATGGGTGAAGGTGGTTCCATCCCA
TTCATGAAAATGTTACATGACCGTTTCAATTGTCAATTTATCGTTACTGGTTTATTAGGT
CCAGGTTCAAATGCTCAGGTCCAAATGAATTCTTAGATATTCCATTTGCCAAGAAATTAT
TAAGTTGTGTATTATTATTAGAGCTCATTCCAAGTTNA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
ifh**n*LNKMSLNIEKVKEFSNKAWDENILPTLSKYIEIPNQSPLYDAEWATNGYTEEA
IQLLEKWILAQGIKGISCEIKRIEGLTPIIFMVVEATKTEKVKTVLLYGHMDKQPPLTDA
WDEGLHPYKAVIKNNKLYGRGGADDGYSTFASISAIKALQEQSIPHDRYVIIIEGSEESG
SIHLPQYVEKFQAEIQIPSLVV---

---QVLDRAENQLTGEVDKRLHVEIPQHRLDQIAKCAEILGETIYDEFSWYKNTTPVTKD
LTQLMINKTWYPTICITGAEGIPHTSNAGNVMRTHTTVKVSVRLPPSFKAADASTVLKEI
FETNPPYGAEVTFKMDKCASGWDSPQTRPELEKALGEASNLFFGKDHVYMGEGGSIPFMK
MLHDRFNCQFIVTGLLGPGSNAQVQMNS*ifhlprny*vvyyy*ssfqv
Translated Amino Acid sequence (All Frames) 
Frame A:
ifh**n*LNKMSLNIEKVKEFSNKAWDENILPTLSKYIEIPNQSPLYDAEWATNGYTEEA
IQLLEKWILAQGIKGISCEIKRIEGLTPIIFMVVEATKTEKVKTVLLYGHMDKQPPLTDA
WDEGLHPYKAVIKNNKLYGRGGADDGYSTFASISAIKALQEQSIPHDRYVIIIEGSEESG
SIHLPQYVEKFQAEIQIPSLVV---

---vkf*ivlkin*lvksikdytlkfhniv*iksqnvlkf*akpsmmnshgtkiplqslk
i*pn**stklgiqlfaspvlkvfhtlqmlvms*eltpplklvfvyhhhskqlmhqly*kk
fsklihhmvlklpskwinvlqvgihhkldqn*kkh*eklqifslakttytwvkvvpshs*
kcymtvsivnlsllvy*vqvqmlrsk*ilrysicqeiiklciiirahskx

Frame B:
fsidkin*ikch*ilkklknlvikhgmkifyqhfqnilkfqinhhymmqngqpmviqkkq
fny*rngs*hkvskvfhvklkelkd*hqlfsw*skqpklkklklyfymvtwinnhhslmh
gmkdyihtkqssritnsmvvvvqmmviqhllqslqskhcknnqfhmivm*lslkvpknlv
qsiyhnmlrssklkfkfhhw*---

---ssfrsc*ksinw*sr*kitr*nsttsfrsnrkmc*nfrrnhl**ilmvqkyhssh*r
fnpiddqqnlvsnylhhrc*rysthfkcw*chenshhr*s*csfttiiqss*cincikrn
frn*stiwc*sylqng*mcfrlgfttn*trirksirrsfksflwqrprihg*rwfhpihe
nvt*pfqlsiyrywfirsrfkcsgpnefldipfakkllscvlllelipsx

Frame C:
fpliklik*nviky*ks*ri***smg*kyftntfkiy*nsksitii*crmgnqwlyrrsn
siiremdlstryqryfm*n*kn*rintnyfhgsrsnqn*ks*nctfiwshg*tttth*cm
g*ritsiqsshqe*qtlwswwcr*wlfnicfnlcnqsiartinst*slcnyh*rfrriwf
npfttic*evps*nsnsiigs---

---QVLDRAENQLTGEVDKRLHVEIPQHRLDQIAKCAEILGETIYDEFSWYKNTTPVTKD
LTQLMINKTWYPTICITGAEGIPHTSNAGNVMRTHTTVKVSVRLPPSFKAADASTVLKEI
FETNPPYGAEVTFKMDKCASGWDSPQTRPELEKALGEASNLFFGKDHVYMGEGGSIPFMK
MLHDRFNCQFIVTGLLGPGSNAQVQMNS*ifhlprny*vvyyy*ssfqv
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFB648 (AFB648Q) /CSM/AF/AFB6-B/AFB648Q.Seq.d/                       2510   0.0
CFG835 (CFG835Q) /CSM/CF/CFG8-B/CFG835Q.Seq.d/                       1338   0.0
AFJ819 (AFJ819Q) /CSM/AF/AFJ8-A/AFJ819Q.Seq.d/                       1318   0.0
SFL182 (SFL182Q) /CSM/SF/SFL1-D/SFL182Q.Seq.d/                       1316   0.0
AFB802 (AFB802Q) /CSM/AF/AFB8-A/AFB802Q.Seq.d/                       1304   0.0
SFH222 (SFH222Q) /CSM/SF/SFH2-A/SFH222Q.Seq.d/                       1296   0.0
SFG389 (SFG389Q) /CSM/SF/SFG3-D/SFG389Q.Seq.d/                       1294   0.0
AFD881 (AFD881Q) /CSM/AF/AFD8-D/AFD881Q.Seq.d/                       1292   0.0
VFI168 (VFI168Q) /CSM/VF/VFI1-C/VFI168Q.Seq.d/                       1283   0.0
CFJ404 (CFJ404Q) /CSM/CF/CFJ4-A/CFJ404Q.Seq.d/                       1283   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BX470224|BX470224.3 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone.    48  0.028  4
AL929031|AL929031.3 Zebrafish DNA sequence from clone CH211-255L18 in linkage group 17.    44  0.057  7
BI435853|BI435853.1 EST538614 P. infestans-challenged leaf Solanum tuberosum cDNA clone PPCCL22 5' sequence, mRNA sequence.    48  0.27  1
BG591390|BG591390.1 EST499232 P. infestans-challenged leaf Solanum tuberosum cDNA clone BPLI8D17 5' sequence, mRNA sequence.    48  0.27  1
BI435340|BI435340.1 EST538101 P. infestans-challenged leaf Solanum tuberosum cDNA clone PPCCD08 5' sequence, mRNA sequence.    48  0.27  1
BQ112173|BQ112173.1 EST597749 mixed potato tissues Solanum tuberosum cDNA clone STMCF59 3' end, mRNA sequence.    48  0.27  1
BX125693|BX125693.1 Danio rerio genomic clone DKEY-71E15, genomic survey sequence.    48  0.27  1
BI435717|BI435717.1 EST538478 P. infestans-challenged leaf Solanum tuberosum cDNA clone PPCCI86 5' sequence, mRNA sequence.    48  0.27  1
BI433279|BI433279.1 EST536040 P. infestans-challenged leaf Solanum tuberosum cDNA clone PPCBC62 5' sequence, mRNA sequence.    48  0.27  1
BQ046631|BQ046631.1 EST595749 P. infestans-challenged potato leaf, incompatible reaction Solanum tuberosum cDNA clone BPLI15C18 5' end, mRNA sequence.    48  0.27  1
dna update 2003. 7.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM743169_1072(AM743169|pid:none) Stenotrophomonas maltophilia K2...   338   2e-92
CP001111_978(CP001111|pid:none) Stenotrophomonas maltophilia R55...   335   3e-91
AE008923_2905(AE008923|pid:none) Xanthomonas axonopodis pv. citr...   335   6e-91
CP000113_526(CP000113|pid:none) Myxococcus xanthus DK 1622, comp...   328   7e-89
AM746676_157(AM746676|pid:none) Sorangium cellulosum 'So ce 56' ...   324   5e-87
CP000733_1810(CP000733|pid:none) Coxiella burnetii Dugway 5J108-...   301   6e-81
AE016828_97(AE016828|pid:none) Coxiella burnetii RSA 493, comple...   299   3e-80
CP001020_1727(CP001020|pid:none) Coxiella burnetii CbuK_Q154, co...   299   3e-80
AM502249_240(AM502249|pid:none) Leishmania infantum chromosome 31.    286   5e-77
CP001053_1440(CP001053|pid:none) Burkholderia phytofirmans PsJN ...   285   1e-76
protein update 2009. 3.27
PSORT 

 psg: 0.53  gvh: 0.29  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.24  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 32.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for AFB648 is nuc
5' end seq. ID AFB648F
5' end seq. 
>AFB648F.Seq
ATTTTCCATTGATAAAATTAATTAAATAAAATGTCATTAAATATTGAAAAAGTTAAAGAA
TTTAGTAATAAAGCATGGGATGAAAATATTTTACCAACACTTTCAAAATATATTGAAATT
CCAAATCAATCACCATTATATGATGCAGAATGGGCAACCAATGGTTATACAGAAGAAGCA
ATTCAATTATTAGAGAAATGGATCTTAGCACAAGGTATCAAAGGTATTTCATGTGAAATT
AAAAGAATTGAAGGATTAACACCAATTATTTTCATGGTAGTCGAAGCAACCAAAACTGAA
AAAGTTAAAACTGTACTTTTATATGGTCACATGGATAAACAACCACCACTCACTGATGCA
TGGGATGAAGGATTACATCCATACAAAGCAGTCATCAAGAATAACAAACTCTATGGTCGT
GGTGGTGCAGATGATGGTTATTCAACATTTGCTTCAATCTCTGCAATCAAAGCATTGCAA
GAACAATCAATTCCACATGATCGTTATGTAATTATCATTGAAGGTTCCGAAGAATCTGGT
TCAATCCATTTACCACAATATGTTGAGAAGTTCCAAGCTGAAATTCAAATTCCATCATTG
GTAGTT----------
Length of 5' end seq. 606
3' end seq. ID AFB648Z
3' end seq. 
>AFB648Z.Seq
----------GTCAAGTTTTAGATCGTGCTGAAAATCAATTAACTGGTGAAGTCGATAAA
AGATTACACGTTGAAATTCCACAACATCGTTTAGATCAAATCGCAAAATGTGCTGAAATT
TTAGGCGAAACCATCTATGATGAATTCTCATGGTACAAAAATACCACTCCAGTCACTAAA
GATTTAACCCAATTGATGATCAACAAAACTTGGTATCCAACTATTTGCATCACCGGTGCT
GAAGGTATTCCACACACTTCAAATGCTGGTAATGTCATGAGAACTCACACCACCGTTAAA
GTTAGTGTTCGTTTACCACCATCATTCAAAGCAGCTGATGCATCAACTGTATTAAAAGAA
ATTTTCGAAACTAATCCACCATATGGTGCTGAAGTTACCTTCAAAATGGATAAATGTGCT
TCAGGTTGGGATTCACCACAAACTAGACCAGAATTAGAAAAAGCATTAGGAGAAGCTTCA
AATCTTTTCTTTGGCAAAGACCACGTATACATGGGTGAAGGTGGTTCCATCCCATTCATG
AAAATGTTACATGACCGTTTCAATTGTCAATTTATCGTTACTGGTTTATTAGGTCCAGGT
TCAAATGCTCAGGTCCAAATGAATTCTTAGATATTCCATTTGCCAAGAAATTATTAAGTT
GTGTATTATTATTAGAGCTCATTCCAAGTTNA
Length of 3' end seq. 682
Connected seq. ID AFB648P
Connected seq. 
>AFB648P.Seq
ATTTTCCATTGATAAAATTAATTAAATAAAATGTCATTAAATATTGAAAAAGTTAAAGAA
TTTAGTAATAAAGCATGGGATGAAAATATTTTACCAACACTTTCAAAATATATTGAAATT
CCAAATCAATCACCATTATATGATGCAGAATGGGCAACCAATGGTTATACAGAAGAAGCA
ATTCAATTATTAGAGAAATGGATCTTAGCACAAGGTATCAAAGGTATTTCATGTGAAATT
AAAAGAATTGAAGGATTAACACCAATTATTTTCATGGTAGTCGAAGCAACCAAAACTGAA
AAAGTTAAAACTGTACTTTTATATGGTCACATGGATAAACAACCACCACTCACTGATGCA
TGGGATGAAGGATTACATCCATACAAAGCAGTCATCAAGAATAACAAACTCTATGGTCGT
GGTGGTGCAGATGATGGTTATTCAACATTTGCTTCAATCTCTGCAATCAAAGCATTGCAA
GAACAATCAATTCCACATGATCGTTATGTAATTATCATTGAAGGTTCCGAAGAATCTGGT
TCAATCCATTTACCACAATATGTTGAGAAGTTCCAAGCTGAAATTCAAATTCCATCATTG
GTAGTT----------GTCAAGTTTTAGATCGTGCTGAAAATCAATTAACTGGTGAAGTC
GATAAAAGATTACACGTTGAAATTCCACAACATCGTTTAGATCAAATCGCAAAATGTGCT
GAAATTTTAGGCGAAACCATCTATGATGAATTCTCATGGTACAAAAATACCACTCCAGTC
ACTAAAGATTTAACCCAATTGATGATCAACAAAACTTGGTATCCAACTATTTGCATCACC
GGTGCTGAAGGTATTCCACACACTTCAAATGCTGGTAATGTCATGAGAACTCACACCACC
GTTAAAGTTAGTGTTCGTTTACCACCATCATTCAAAGCAGCTGATGCATCAACTGTATTA
AAAGAAATTTTCGAAACTAATCCACCATATGGTGCTGAAGTTACCTTCAAAATGGATAAA
TGTGCTTCAGGTTGGGATTCACCACAAACTAGACCAGAATTAGAAAAAGCATTAGGAGAA
GCTTCAAATCTTTTCTTTGGCAAAGACCACGTATACATGGGTGAAGGTGGTTCCATCCCA
TTCATGAAAATGTTACATGACCGTTTCAATTGTCAATTTATCGTTACTGGTTTATTAGGT
CCAGGTTCAAATGCTCAGGTCCAAATGAATTCTTAGATATTCCATTTGCCAAGAAATTAT
TAAGTTGTGTATTATTATTAGAGCTCATTCCAAGTTNA
Length of connected seq. 1288
Full length Seq ID -
Full length Seq. -
Length of full length seq. -