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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHG350
Library VH
(Link to library)
Clone ID VHG350
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16508-1
Original site URL
Representative seq. ID VHG350P
(Link to Original site)
Representative DNA sequence 
>VHG350 (VHG350Q) /CSM/VH/VHG3-C/VHG350Q.Seq.d/
TTATCGGTCGTATGGATTATCAAGATATCGAAGCTCGTTTAGAGAATAAGCAAATGGAGT
TCATGTGGAGATCGACCCAAAGTACACCAGAGAATCAAGTTTTCACATCAGTACTACGTG
CAATGTATTGTACTCCAGATGGTTTCAACTTTGAGCAAGGTGATGACCCAATTCAAGATG
ACCCAAATTTATTCGATAATAACGTAGACTCAAGAGCTGAACAGTTCACTCAAGTGGCAC
TTGAATATGCAACTCATTATCGTACCAATAATGTACTAATTCCATTTGGTTGTGATTTCG
CTTACCTTAACGCTCAAATGTATTACAAAAACATTGACAAACTAATCGCACATATCAATT
CAAATCCGGATAAATATGGTTTAAATTTGTTATATTCAACTCCATCAATTTATATAGGTG
CTGTAAATGATGCCAATCTAGTATGGGAAGTGAAAACTGATGATCTCTTCCCATATGCTG
ATAATGAATTTAGTTATTGGACTGGTTATTTCGTTAGTCGTCCAGCATTGAAAGGCTACG
TTCGTCAAAATAATGCTCTATTACATGTAGTTGAACAAATGTTGGXXXXXXXXXXTGGTC
AACCAATGAATGAGTCAACTCAAATCGTTACAACTAGTAAATTGATTTTCCATGATATTT
CAAGTTATGCTCAATCTCATTATCGTCCTGCTGCACTCTCTTTATCACATCCATTACTCC
CAATGTTTACAACCACTCAACAATCTTCAAACGATTGGAATTCTCAATATCAAGGTGTTT
ACTCACCATTGACTTCAGCCTCACCTTTACCAAATGGTTTAAAGATTCAAACTCTACAAT
GGTTAGATAATCAAGATAATACCATACTTTTACGTATTGAAAATATTTATCAAATTGATG
GTCAAGATTCACAAGATCCACAAACTATCACTTTAGATCTTTCAACTATCTTTTCAACAA
TTACAATCACTTCTGCAACTGAAATGAATTTAACTGGTGTTCAAAAATTATCAAATTTAT
CAAGATTAAAATGGAAAACTGTTGATGGTAAAAACTATGATCATAAATCATCATCATCAA
CTAAAGAAGATTCTTCAAAGGTTTTGTTTTTACTTTTTCTCCAATGCAAATCAGAACTTT
TATAATTACTAC
sequence update 2002.10.25
Translated Amino Acid sequence 
IGRMDYQDIEARLENKQMEFMWRSTQSTPENQVFTSVLRAMYCTPDGFNFEQGDDPIQDD
PNLFDNNVDSRAEQFTQVALEYATHYRTNNVLIPFGCDFAYLNAQMYYKNIDKLIAHINS
NPDKYGLNLLYSTPSIYIGAVNDANLVWEVKTDDLFPYADNEFSYWTGYFVSRPALKGYV
RQNNALLHVVEQML---

---GQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHPLLPMFTTTQQSSNDWNSQ
YQGVYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQTITLDLST
IFSTITITSATEMNLTGVQKLSNLSRLKWKTVDGKNYDHKSSSSTKEDSSKVLFLLFLQC
KSELL*ll
Translated Amino Acid sequence (All Frames) 
Frame A:
lsvvwiikisklv*riskwsscgdrpkvhqrikfshqyyvqcivlqmvstlskvmtqfkm
tqiysiit*tqelnsslkwhlnmqliivpimy*fhlvvisltltlkcitktltn*shisi
qirinmv*icyiqlhqfi*vl*mmpi*ygk*klmisshmlimnlviglvislvvqh*kat
fvkimlyym*lnkcw---

---wstne*vnsnryn**idfp*yfklcsislsscctlfitsitpnvynhstifkrlefs
isrclltidfsltftkwfkdsnstmvr*sr*yhtfty*kylsn*wsrftrstnyhfrsfn
ylfnnynhfcn*nefnwcskiikfikikmenc*w*kl*s*iiiin*rrffkgfvftfspm
qirtfiit

Frame B:
yrsyglsryrssfre*angvhveidpkytressfhisttcnvlysrwfql*ar**pnsr*
pkfir**rrlks*tvhssgt*icnslsyq*ctnsiwl*frlp*rsnvlqkh*qtnrtyqf
ksg*iwfkfvifnsinlyrcck*cqssmgsen**slpic***i*lldwlfr*sssierlr
ssk*csitcs*tnv---

---GQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHPLLPMFTTTQQSSNDWNSQ
YQGVYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQTITLDLST
IFSTITITSATEMNLTGVQKLSNLSRLKWKTVDGKNYDHKSSSSTKEDSSKVLFLLFLQC
KSELL*ll

Frame C:
IGRMDYQDIEARLENKQMEFMWRSTQSTPENQVFTSVLRAMYCTPDGFNFEQGDDPIQDD
PNLFDNNVDSRAEQFTQVALEYATHYRTNNVLIPFGCDFAYLNAQMYYKNIDKLIAHINS
NPDKYGLNLLYSTPSIYIGAVNDANLVWEVKTDDLFPYADNEFSYWTGYFVSRPALKGYV
RQNNALLHVVEQML---

---vnq*msqlkslqlvn*fsmifqvmlnliivllhslyhihysqclqplnnlqtigiln
ikvfthh*lqphlyqmv*rfklyng*iikiipyfyvlkifiklmvkihkihklsl*ifql
sfqqlqsllqlk*i*lvfknyqiyqd*ngkllmvktmiinhhhqlkkilqrfcfyffsna
nqnfynyy
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHG350 (VHG350Q) /CSM/VH/VHG3-C/VHG350Q.Seq.d/                       2224   0.0
VHP791 (VHP791Q) /CSM/VH/VHP7-D/VHP791Q.Seq.d/                       1152   0.0
VHK605 (VHK605Q) /CSM/VH/VHK6-A/VHK605Q.Seq.d/                       1152   0.0
VHK142 (VHK142Q) /CSM/VH/VHK1-B/VHK142Q.Seq.d/                       1152   0.0
VHJ346 (VHJ346Q) /CSM/VH/VHJ3-B/VHJ346Q.Seq.d/                       1152   0.0
VHG861 (VHG861Q) /CSM/VH/VHG8-C/VHG861Q.Seq.d/                       1152   0.0
VHG565 (VHG565Q) /CSM/VH/VHG5-C/VHG565Q.Seq.d/                       1152   0.0
VHG518 (VHG518Q) /CSM/VH/VHG5-A/VHG518Q.Seq.d/                       1152   0.0
VHF343 (VHF343Q) /CSM/VH/VHF3-B/VHF343Q.Seq.d/                       1152   0.0
VHD601 (VHD601Q) /CSM/VH/VHD6-A/VHD601Q.Seq.d/                       1152   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ421692) Dictyostelium discoideum cDNA clone:ddv43d13, 5' ...  1156   0.0    1
(M82822) Dictyostelium discoideum alpha-mannosidase (manA) g...  1148   0.0    1
(BJ427560) Dictyostelium discoideum cDNA clone:ddv62f23, 5' ...  1148   0.0    1
(BJ424331) Dictyostelium discoideum cDNA clone:ddv52i02, 5' ...  1148   0.0    1
(BJ424061) Dictyostelium discoideum cDNA clone:ddv51c11, 5' ...  1148   0.0    1
(BJ423499) Dictyostelium discoideum cDNA clone:ddv49l11, 5' ...  1148   0.0    1
(BJ422037) Dictyostelium discoideum cDNA clone:ddv44j16, 5' ...  1148   0.0    1
(BJ421993) Dictyostelium discoideum cDNA clone:ddv44a17, 5' ...  1148   0.0    1
(BJ421843) Dictyostelium discoideum cDNA clone:ddv44c05, 5' ...  1148   0.0    1
(BJ420984) Dictyostelium discoideum cDNA clone:ddv41f11, 5' ...  1148   0.0    1
dna update 2008.11. 2
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

M82822_1(M82822|pid:none) Dictyostelium discoideum alpha-mannosi...   409   e-112
(P34098) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   409   e-112
BC091843_1(BC091843|pid:none) Danio rerio zgc:110815, mRNA (cDNA...   185   2e-45
BC121882_1(BC121882|pid:none) Xenopus tropicalis lysosomal alpha...   171   6e-41
AE014134_1973(AE014134|pid:none) Drosophila melanogaster chromos...   167   6e-40
AC148217_12(AC148217|pid:none) Medicago truncatula clone mth2-20...   167   6e-40
AY119107_1(AY119107|pid:none) Drosophila melanogaster RE08556 fu...   164   4e-39
AE014134_1633(AE014134|pid:none) Drosophila melanogaster chromos...   164   4e-39
AB026648_12(AB026648|pid:none) Arabidopsis thaliana genomic DNA,...   162   3e-38
AY099704_1(AY099704|pid:none) Arabidopsis thaliana alpha-mannosi...   157   5e-37
protein update 2009. 7.12
PSORT 

 psg: 0.75  gvh: 0.35  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.22  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 40.0 %: nuclear
	  8.0 %: mitochondrial
	  8.0 %: vacuolar
	  4.0 %: peroxisomal

>> prediction for VHG350 is cyt
5' end seq. ID VHG350F
5' end seq. 
>VHG350F.Seq
TTATCGGTCGTATGGATTATCAAGATATCGAAGCTCGTTTAGAGAATAAGCAAATGGAGT
TCATGTGGAGATCGACCCAAAGTACACCAGAGAATCAAGTTTTCACATCAGTACTACGTG
CAATGTATTGTACTCCAGATGGTTTCAACTTTGAGCAAGGTGATGACCCAATTCAAGATG
ACCCAAATTTATTCGATAATAACGTAGACTCAAGAGCTGAACAGTTCACTCAAGTGGCAC
TTGAATATGCAACTCATTATCGTACCAATAATGTACTAATTCCATTTGGTTGTGATTTCG
CTTACCTTAACGCTCAAATGTATTACAAAAACATTGACAAACTAATCGCACATATCAATT
CAAATCCGGATAAATATGGTTTAAATTTGTTATATTCAACTCCATCAATTTATATAGGTG
CTGTAAATGATGCCAATCTAGTATGGGAAGTGAAAACTGATGATCTCTTCCCATATGCTG
ATAATGAATTTAGTTATTGGACTGGTTATTTCGTTAGTCGTCCAGCATTGAAAGGCTACG
TTCGTCAAAATAATGCTCTATTACATGTAGTTGAACAAATGTTGGNNNNNNNNNN
Length of 5' end seq. 595
3' end seq. ID VHG350Z
3' end seq. 
>VHG350Z.Seq
NNNNNNNNNNTGGTCAACCAATGAATGAGTCAACTCAAATCGTTACAACTAGTAAATTGA
TTTTCCATGATATTTCAAGTTATGCTCAATCTCATTATCGTCCTGCTGCACTCTCTTTAT
CACATCCATTACTCCCAATGTTTACAACCACTCAACAATCTTCAAACGATTGGAATTCTC
AATATCAAGGTGTTTACTCACCATTGACTTCAGCCTCACCTTTACCAAATGGTTTAAAGA
TTCAAACTCTACAATGGTTAGATAATCAAGATAATACCATACTTTTACGTATTGAAAATA
TTTATCAAATTGATGGTCAAGATTCACAAGATCCACAAACTATCACTTTAGATCTTTCAA
CTATCTTTTCAACAATTACAATCACTTCTGCAACTGAAATGAATTTAACTGGTGTTCAAA
AATTATCAAATTTATCAAGATTAAAATGGAAAACTGTTGATGGTAAAAACTATGATCATA
AATCATCATCATCAACTAAAGAAGATTCTTCAAAGGTTTTGTTTTTACTTTTTCTCCAAT
GCAAATCAGAACTTTTATAATTACTAC
Length of 3' end seq. 567
Connected seq. ID VHG350P
Connected seq. 
>VHG350P.Seq
TTATCGGTCGTATGGATTATCAAGATATCGAAGCTCGTTTAGAGAATAAGCAAATGGAGT
TCATGTGGAGATCGACCCAAAGTACACCAGAGAATCAAGTTTTCACATCAGTACTACGTG
CAATGTATTGTACTCCAGATGGTTTCAACTTTGAGCAAGGTGATGACCCAATTCAAGATG
ACCCAAATTTATTCGATAATAACGTAGACTCAAGAGCTGAACAGTTCACTCAAGTGGCAC
TTGAATATGCAACTCATTATCGTACCAATAATGTACTAATTCCATTTGGTTGTGATTTCG
CTTACCTTAACGCTCAAATGTATTACAAAAACATTGACAAACTAATCGCACATATCAATT
CAAATCCGGATAAATATGGTTTAAATTTGTTATATTCAACTCCATCAATTTATATAGGTG
CTGTAAATGATGCCAATCTAGTATGGGAAGTGAAAACTGATGATCTCTTCCCATATGCTG
ATAATGAATTTAGTTATTGGACTGGTTATTTCGTTAGTCGTCCAGCATTGAAAGGCTACG
TTCGTCAAAATAATGCTCTATTACATGTAGTTGAACAAATGTTGG----------TGGTC
AACCAATGAATGAGTCAACTCAAATCGTTACAACTAGTAAATTGATTTTCCATGATATTT
CAAGTTATGCTCAATCTCATTATCGTCCTGCTGCACTCTCTTTATCACATCCATTACTCC
CAATGTTTACAACCACTCAACAATCTTCAAACGATTGGAATTCTCAATATCAAGGTGTTT
ACTCACCATTGACTTCAGCCTCACCTTTACCAAATGGTTTAAAGATTCAAACTCTACAAT
GGTTAGATAATCAAGATAATACCATACTTTTACGTATTGAAAATATTTATCAAATTGATG
GTCAAGATTCACAAGATCCACAAACTATCACTTTAGATCTTTCAACTATCTTTTCAACAA
TTACAATCACTTCTGCAACTGAAATGAATTTAACTGGTGTTCAAAAATTATCAAATTTAT
CAAGATTAAAATGGAAAACTGTTGATGGTAAAAACTATGATCATAAATCATCATCATCAA
CTAAAGAAGATTCTTCAAAGGTTTTGTTTTTACTTTTTCTCCAATGCAAATCAGAACTTT
TATAATTACTAC
Length of connected seq. 1142
Full length Seq ID -
Full length Seq. -
Length of full length seq. -