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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFB645
Library AF
(Link to library)
Clone ID AFB645
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16294-1
Original site URL
Representative seq. ID AFB645P
(Link to Original site)
Representative DNA sequence 
>AFB645 (AFB645Q) /CSM/AF/AFB6-B/AFB645Q.Seq.d/
ATTTTTTATTTTATTTTCNATTGATAAAATNAATTAAATAAAATGTCATTAAATATTGAA
AAAGTTAAAGAATTTAGTAATAAAGCATGGGATGAAAATATTTTACCAACACTTTCAAAA
TATATTGAAATTCCAAATCAATCACCATTATATGATGCAGAATGGGCAACCAATGGTTAT
ACAGAAGAAGCAATTCAATTATTAGAGAAATGGATCTTAGCACAAGGTATCAAAGGTATT
TCATGTGAAATTAAAAGAATTGAAGGATTAACACCAATTATTTTCATGGTAGTCGAAGCA
ACCAAAACTGAAAAAGTTAAAACTGTACTTTTATATGGTCACATGGATAAACAACCACCA
CTCACTGATGCATGGGATGAAGGATTACATTCATACAAAGCAGTCATCAAGAATAACAAA
CTCTATGGTCGTGGTGGTGCAGATGATGGTTATTCAACATTTGCTTCAATCTCTGCAATC
AAAGCATTGCAAGAACAATCAATTCCACATGATCGTTATGTAATTATCATTGAAGGTTCC
GAAGAATCTGGTTCAATCCATTTACCACAATATGTTGAGAAGTTCCAAGCTGAAATTCAA
ATTCCATCATTGGTAGTTTGXXXXXXXXXXTAAAAGATTACACGTTGAAATTCCACAACA
TCGTTTAGATCAAATCGCAAAATGTGCTGAAATTTTAGGCGAAACCATCTATGATGAATT
CTCATGGTACAAAAATACCACTCCAGTCACTAAAGATTTAACCCAATTGATGATCAACAA
AACTTGGTATCCAACTATTTGCATCACCGGTGCTGAAGGTATTCCACACACTTCAAATGC
TGGTAATGTCATGAGAACTCACACCACCGTTAAAGTTAGTGTTCGTTTACCACCATCATT
CAAAGCAGCTGATGCATCAACTGTATTAAAAGAAATTTTCGAAACTAATCCACCATATGG
TGCTGAAGTTACCTTCAAAATGGATAAATGTGCTTCAGGTTGGGATTCACCACAAACTAG
ACCAGAATTAGAAAAAGCATTAGGAGAAGCTTCAAATCTTTTCTTTGGCAAAGACCACGT
ATACATGGGTGAAGGTGGTTCCATCCCATTCATGAAAATGTTACATGACCGTTTCAATTG
TCAATTTATCGTTACTGGTTTATTAGGTCCAGGTTCAAATGCTCATGGTCCAAATNAATT
CTTAGATATTCCATTTGCCAAGAAATTATTAAGTTGTGTATTATTTATTAGAGCTCATTC
CAAGTT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
ifyfifx**NXLNKMSLNIEKVKEFSNKAWDENILPTLSKYIEIPNQSPLYDAEWATNGY
TEEAIQLLEKWILAQGIKGISCEIKRIEGLTPIIFMVVEATKTEKVKTVLLYGHMDKQPP
LTDAWDEGLHSYKAVIKNNKLYGRGGADDGYSTFASISAIKALQEQSIPHDRYVIIIEGS
EESGSIHLPQYVEKFQAEIQIPSLVV---

---KRLHVEIPQHRLDQIAKCAEILGETIYDEFSWYKNTTPVTKDLTQLMINKTWYPTIC
ITGAEGIPHTSNAGNVMRTHTTVKVSVRLPPSFKAADASTVLKEIFETNPPYGAEVTFKM
DKCASGWDSPQTRPELEKALGEASNLFFGKDHVYMGEGGSIPFMKMLHDRFNCQFIVTGL
LGPGSNAHGPNXFLDIPFAKKLLSCVLFIRAHSK
Translated Amino Acid sequence (All Frames) 
Frame A:
ifyfifx**NXLNKMSLNIEKVKEFSNKAWDENILPTLSKYIEIPNQSPLYDAEWATNGY
TEEAIQLLEKWILAQGIKGISCEIKRIEGLTPIIFMVVEATKTEKVKTVLLYGHMDKQPP
LTDAWDEGLHSYKAVIKNNKLYGRGGADDGYSTFASISAIKALQEQSIPHDRYVIIIEGS
EESGSIHLPQYVEKFQAEIQIPSLVV---

---*kitr*nsttsfrsnrkmc*nfrrnhl**ilmvqkyhssh*rfnpiddqqnlvsnyl
hhrc*rysthfkcw*chenshhr*s*csfttiiqss*cincikrnfrn*stiwc*sylqn
g*mcfrlgfttn*trirksirrsfksflwqrprihg*rwfhpihenvt*pfqlsiyrywf
irsrfkcswskxilrysicqeiiklciiy*ssfqv

Frame B:
ffilfsidkxn*ikch*ilkklknlvikhgmkifyqhfqnilkfqinhhymmqngqpmvi
qkkqfny*rngs*hkvskvfhvklkelkd*hqlfsw*skqpklkklklyfymvtwinnhh
slmhgmkdyihtkqssritnsmvvvvqmmviqhllqslqskhcknnqfhmivm*lslkvp
knlvqsiyhnmlrssklkfkfhhw*f---

---KRLHVEIPQHRLDQIAKCAEILGETIYDEFSWYKNTTPVTKDLTQLMINKTWYPTIC
ITGAEGIPHTSNAGNVMRTHTTVKVSVRLPPSFKAADASTVLKEIFETNPPYGAEVTFKM
DKCASGWDSPQTRPELEKALGEASNLFFGKDHVYMGEGGSIPFMKMLHDRFNCQFIVTGL
LGPGSNAHGPNXFLDIPFAKKLLSCVLFIRAHSK

Frame C:
flfyfxlikxik*nviky*ks*ri***smg*kyftntfkiy*nsksitii*crmgnqwly
rrsnsiiremdlstryqryfm*n*kn*rintnyfhgsrsnqn*ks*nctfiwshg*tttt
h*cmg*ritfiqsshqe*qtlwswwcr*wlfnicfnlcnqsiartinst*slcnyh*rfr
riwfnpfttic*evps*nsnsiigsl---

---kdytlkfhniv*iksqnvlkf*akpsmmnshgtkiplqslki*pn**stklgiqlfa
spvlkvfhtlqmlvms*eltpplklvfvyhhhskqlmhqly*kkfsklihhmvlklpskw
invlqvgihhkldqn*kkh*eklqifslakttytwvkvvpshs*kcymtvsivnlsllvy
*vqvqmlmvqxns*ifhlprny*vvyyllelips
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFB645 (AFB645Q) /CSM/AF/AFB6-B/AFB645Q.Seq.d/                       2401   0.0
SFL182 (SFL182Q) /CSM/SF/SFL1-D/SFL182Q.Seq.d/                       1239   0.0
SFG389 (SFG389Q) /CSM/SF/SFG3-D/SFG389Q.Seq.d/                       1229   0.0
AFI435 (AFI435Q) /CSM/AF/AFI4-B/AFI435Q.Seq.d/                       1229   0.0
AFD881 (AFD881Q) /CSM/AF/AFD8-D/AFD881Q.Seq.d/                       1227   0.0
AFL393 (AFL393Q) /CSM/AF/AFL3-D/AFL393Q.Seq.d/                       1225   0.0
CFH871 (CFH871Q) /CSM/CF/CFH8-C/CFH871Q.Seq.d/                       1223   0.0
CFG835 (CFG835Q) /CSM/CF/CFG8-B/CFG835Q.Seq.d/                       1223   0.0
AFB648 (AFB648Q) /CSM/AF/AFB6-B/AFB648Q.Seq.d/                       1223   0.0
AFB772 (AFB772Q) /CSM/AF/AFB7-C/AFB772Q.Seq.d/                       1221   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BX470224|BX470224.3 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone.    48  0.025  3
AZ671598|AZ671598.1 ENTKM66TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    40  0.16  2
BQ112173|BQ112173.1 EST597749 mixed potato tissues Solanum tuberosum cDNA clone STMCF59 3' end, mRNA sequence.    48  0.27  1
BI435340|BI435340.1 EST538101 P. infestans-challenged leaf Solanum tuberosum cDNA clone PPCCD08 5' sequence, mRNA sequence.    48  0.27  1
BX125693|BX125693.1 Danio rerio genomic clone DKEY-71E15, genomic survey sequence.    48  0.27  1
BI433279|BI433279.1 EST536040 P. infestans-challenged leaf Solanum tuberosum cDNA clone PPCBC62 5' sequence, mRNA sequence.    48  0.27  1
BI435717|BI435717.1 EST538478 P. infestans-challenged leaf Solanum tuberosum cDNA clone PPCCI86 5' sequence, mRNA sequence.    48  0.27  1
BQ046631|BQ046631.1 EST595749 P. infestans-challenged potato leaf, incompatible reaction Solanum tuberosum cDNA clone BPLI15C18 5' end, mRNA sequence.    48  0.27  1
BI435853|BI435853.1 EST538614 P. infestans-challenged leaf Solanum tuberosum cDNA clone PPCCL22 5' sequence, mRNA sequence.    48  0.27  1
BG591390|BG591390.1 EST499232 P. infestans-challenged leaf Solanum tuberosum cDNA clone BPLI8D17 5' sequence, mRNA sequence.    48  0.27  1
dna update 2003. 7.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM743169_1072(AM743169|pid:none) Stenotrophomonas maltophilia K2...   204   4e-51
AM039952_3097(AM039952|pid:none) Xanthomonas campestris pv. vesi...   204   7e-51
AM746676_157(AM746676|pid:none) Sorangium cellulosum 'So ce 56' ...   204   7e-51
AE008923_2905(AE008923|pid:none) Xanthomonas axonopodis pv. citr...   204   7e-51
CP001111_978(CP001111|pid:none) Stenotrophomonas maltophilia R55...   203   9e-51
CP000113_526(CP000113|pid:none) Myxococcus xanthus DK 1622, comp...   203   9e-51
DQ384330_1(DQ384330|pid:none) Hartmannella vermiformis succinyl-...   199   2e-49
DQ384331_1(DQ384331|pid:none) Physarum polycephalum succinyl-dia...   192   3e-47
AE016828_97(AE016828|pid:none) Coxiella burnetii RSA 493, comple...   189   2e-46
CP001020_1727(CP001020|pid:none) Coxiella burnetii CbuK_Q154, co...   189   2e-46
protein update 2009. 3.27
PSORT 

 psg: 0.42  gvh: 0.29  alm: 0.35  top: 0.53  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 28.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for AFB645 is nuc
5' end seq. ID AFB645F
5' end seq. 
>AFB645F.Seq
ATTTTTTATTTTATTTTCNATTGATAAAATNAATTAAATAAAATGTCATTAAATATTGAA
AAAGTTAAAGAATTTAGTAATAAAGCATGGGATGAAAATATTTTACCAACACTTTCAAAA
TATATTGAAATTCCAAATCAATCACCATTATATGATGCAGAATGGGCAACCAATGGTTAT
ACAGAAGAAGCAATTCAATTATTAGAGAAATGGATCTTAGCACAAGGTATCAAAGGTATT
TCATGTGAAATTAAAAGAATTGAAGGATTAACACCAATTATTTTCATGGTAGTCGAAGCA
ACCAAAACTGAAAAAGTTAAAACTGTACTTTTATATGGTCACATGGATAAACAACCACCA
CTCACTGATGCATGGGATGAAGGATTACATTCATACAAAGCAGTCATCAAGAATAACAAA
CTCTATGGTCGTGGTGGTGCAGATGATGGTTATTCAACATTTGCTTCAATCTCTGCAATC
AAAGCATTGCAAGAACAATCAATTCCACATGATCGTTATGTAATTATCATTGAAGGTTCC
GAAGAATCTGGTTCAATCCATTTACCACAATATGTTGAGAAGTTCCAAGCTGAAATTCAA
ATTCCATCATTGGTAGTTTG----------
Length of 5' end seq. 620
3' end seq. ID AFB645Z
3' end seq. 
>AFB645Z.Seq
----------TAAAAGATTACACGTTGAAATTCCACAACATCGTTTAGATCAAATCGCAA
AATGTGCTGAAATTTTAGGCGAAACCATCTATGATGAATTCTCATGGTACAAAAATACCA
CTCCAGTCACTAAAGATTTAACCCAATTGATGATCAACAAAACTTGGTATCCAACTATTT
GCATCACCGGTGCTGAAGGTATTCCACACACTTCAAATGCTGGTAATGTCATGAGAACTC
ACACCACCGTTAAAGTTAGTGTTCGTTTACCACCATCATTCAAAGCAGCTGATGCATCAA
CTGTATTAAAAGAAATTTTCGAAACTAATCCACCATATGGTGCTGAAGTTACCTTCAAAA
TGGATAAATGTGCTTCAGGTTGGGATTCACCACAAACTAGACCAGAATTAGAAAAAGCAT
TAGGAGAAGCTTCAAATCTTTTCTTTGGCAAAGACCACGTATACATGGGTGAAGGTGGTT
CCATCCCATTCATGAAAATGTTACATGACCGTTTCAATTGTCAATTTATCGTTACTGGTT
TATTAGGTCCAGGTTCAAATGCTCATGGTCCAAATNAATTCTTAGATATTCCATTTGCCA
AGAAATTATTAAGTTGTGTATTATTTATTAGAGCTCATTCCAAGTT
Length of 3' end seq. 636
Connected seq. ID AFB645P
Connected seq. 
>AFB645P.Seq
ATTTTTTATTTTATTTTCNATTGATAAAATNAATTAAATAAAATGTCATTAAATATTGAA
AAAGTTAAAGAATTTAGTAATAAAGCATGGGATGAAAATATTTTACCAACACTTTCAAAA
TATATTGAAATTCCAAATCAATCACCATTATATGATGCAGAATGGGCAACCAATGGTTAT
ACAGAAGAAGCAATTCAATTATTAGAGAAATGGATCTTAGCACAAGGTATCAAAGGTATT
TCATGTGAAATTAAAAGAATTGAAGGATTAACACCAATTATTTTCATGGTAGTCGAAGCA
ACCAAAACTGAAAAAGTTAAAACTGTACTTTTATATGGTCACATGGATAAACAACCACCA
CTCACTGATGCATGGGATGAAGGATTACATTCATACAAAGCAGTCATCAAGAATAACAAA
CTCTATGGTCGTGGTGGTGCAGATGATGGTTATTCAACATTTGCTTCAATCTCTGCAATC
AAAGCATTGCAAGAACAATCAATTCCACATGATCGTTATGTAATTATCATTGAAGGTTCC
GAAGAATCTGGTTCAATCCATTTACCACAATATGTTGAGAAGTTCCAAGCTGAAATTCAA
ATTCCATCATTGGTAGTTTG----------TAAAAGATTACACGTTGAAATTCCACAACA
TCGTTTAGATCAAATCGCAAAATGTGCTGAAATTTTAGGCGAAACCATCTATGATGAATT
CTCATGGTACAAAAATACCACTCCAGTCACTAAAGATTTAACCCAATTGATGATCAACAA
AACTTGGTATCCAACTATTTGCATCACCGGTGCTGAAGGTATTCCACACACTTCAAATGC
TGGTAATGTCATGAGAACTCACACCACCGTTAAAGTTAGTGTTCGTTTACCACCATCATT
CAAAGCAGCTGATGCATCAACTGTATTAAAAGAAATTTTCGAAACTAATCCACCATATGG
TGCTGAAGTTACCTTCAAAATGGATAAATGTGCTTCAGGTTGGGATTCACCACAAACTAG
ACCAGAATTAGAAAAAGCATTAGGAGAAGCTTCAAATCTTTTCTTTGGCAAAGACCACGT
ATACATGGGTGAAGGTGGTTCCATCCCATTCATGAAAATGTTACATGACCGTTTCAATTG
TCAATTTATCGTTACTGGTTTATTAGGTCCAGGTTCAAATGCTCATGGTCCAAATNAATT
CTTAGATATTCCATTTGCCAAGAAATTATTAAGTTGTGTATTATTTATTAGAGCTCATTC
CAAGTT
Length of connected seq. 1256
Full length Seq ID -
Full length Seq. -
Length of full length seq. -