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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHG280
Library VH
(Link to library)
Clone ID VHG280
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12022-1|Contig-U13289-1
Original site URL
Representative seq. ID VHG280P
(Link to Original site)
Representative DNA sequence 
>VHG280 (VHG280Q) /CSM/VH/VHG2-D/VHG280Q.Seq.d/
AACTCGTTCACTTATTTTGTTGTTCGATAATATTATTTTATTTATTTATTTTATTATAAC
TATTTTTGTTGTTGGATTGTTGTAATATTTATTTTAAATAATAAATATAAATAATAAAAT
AAAAATGTTAAAATTATTACGTAATAATGGAATAAATAAATTAAAATCTAATTTAATTAG
AAATTATTCAACAAAACAATCAATATTTCAAGCATTAGATACATTTCCAAAAAGACATAT
TGGACCAAATGAAAATGAAATTAATGAAATGTTAAAAAGTATTAATACATCAAAACTTTC
AAAAAAGAATCCAAATAGTTTAGAACAATTGATCGAATATACAATTCCAAAGGATATTAG
ATTAAATAGAGAATTAAATATTGAAGAGAATAAAGTTATTGGTGAGAATCAATTATTAAA
GGATTTAAAAAAGATTGCAGAAAAGAATAAAGTTTATAGATCATTCATTGGCATGGGTTA
TTATGGAACAATTACACCACATGTAATTCAACGTAACATTTTAGAGAXXXXXXXXXXAGC
AACCCAAGTCGCCATTCTCAATGCAAACTATATGGCAAGTAGATTAAAGGATCACTATAA
AATTCTTTACACTGGTTCCCATGGTTTGGTAGCTCATGAATTCATTATTGATCTTAGAAT
GTTTAAGGAATCTGCTGGTATCGAAGCTGAAGATGTAGCTAAACGTCTTCAAGATATGAA
TTTCCATGGTCCAACCATGTCTTGGCCAGTACCAAATACTCTTATGATTGAACCAACCGA
ATCTGAATCAAAATATGAATTGGATCGTCTTTGTGATGCTCTCATTTTAATTCGTGAGGA
AATTAGAGAGATTGAAACTGGTAAAGCCGACAGAAAAAATAATGTTCTCGTTAATTCTCC
ACACACTGAAAAAGTTATTGTCGCTGATAATTGGAATTATCCATACTCTCGTTCTAAAGC
TGCTTTCCCAACTCCTGCAACTGTTGCCTCAAAATTCTGGCCAACCGTTGGTAGAATTGA
TAACGTTCATGGCGATAAAAATTTAGTTTGTTCNTGCCCACCACTTTCNCATTATCAATA
AAATAAAATAAAAAATTAAATAAATA
sequence update 2002.10.25
Translated Amino Acid sequence 
trslillfdniilfiyfiitifvvgll*ylf*IININNKIKMLKLLRNNGINKLKSNLIR
NYSTKQSIFQALDTFPKRHIGPNENEINEMLKSINTSKLSKKNPNSLEQLIEYTIPKDIR
LNRELNIEENKVIGENQLLKDLKKIAEKNKVYRSFIGMGYYGTITPHVIQRNILE---

---ATQVAILNANYMASRLKDHYKILYTGSHGLVAHEFIIDLRMFKESAGIEAEDVAKRL
QDMNFHGPTMSWPVPNTLMIEPTESESKYELDRLCDALILIREEIREIETGKADRKNNVL
VNSPHTEKVIVADNWNYPYSRSKAAFPTPATVASKFWPTVGRIDNVHGDKNLVCSCPPLS
HYQ*nkikn*in
Translated Amino Acid sequence (All Frames) 
Frame A:
nsftyfvvr*yyfiylfyynyfccwivvifilnnkyk**nknvkiit**wnk*iki*fn*
klfnktinissiryiskktywtk*k*n**nvkky*yiktfkkesk*frtidriynskgy*
ik*riky*re*syw*esiikgfkkdcrke*sl*iihwhgllwnnyttcnst*hfr---

---snpsrhsqcklygk*ikgsl*nslhwfpwfgss*ihy*s*nv*gicwyrs*rcs*ts
sryefpwsnhvlastkysyd*tnri*iki*igssl*cshfns*gn*rd*nw*srqkk*cs
r*fsth*ksycr**lelsilsf*scfpnscncclkilanrw*n**rswr*kfslfxpttf
xlsik*nkklnk

Frame B:
trslillfdniilfiyfiitifvvgll*ylf*IININNKIKMLKLLRNNGINKLKSNLIR
NYSTKQSIFQALDTFPKRHIGPNENEINEMLKSINTSKLSKKNPNSLEQLIEYTIPKDIR
LNRELNIEENKVIGENQLLKDLKKIAEKNKVYRSFIGMGYYGTITPHVIQRNILE---

---ATQVAILNANYMASRLKDHYKILYTGSHGLVAHEFIIDLRMFKESAGIEAEDVAKRL
QDMNFHGPTMSWPVPNTLMIEPTESESKYELDRLCDALILIREEIREIETGKADRKNNVL
VNSPHTEKVIVADNWNYPYSRSKAAFPTPATVASKFWPTVGRIDNVHGDKNLVCSCPPLS
HYQ*nkikn*in

Frame C:
lvhlfccsiilfylfill*lfllldccniyfk**i*iik*kc*nyyvime*in*nli*le
iiqqnnqyfkh*ihfqkdildqmkmklmkc*kvlihqnfqkriqiv*nn*sniqfqrild
*ien*ilkrikllvriny*ri*krlqkrikfidhslawvimeqlhhm*fnvtf*r---

---qpkspfsmqtiwqvd*ritikfftlvpmvw*lmnsllileclrnllvsklkm*lnvf
ki*ismvqpclgqyqill*lnqpnlnqnmnwivfvmlsf*fvrklerlklvkptekimfs
lilhtlkkllsliigiihtlvlkllsqllqllpqnsgqplvelitfmaiki*fvxahhfx
iinkik*kik*i
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHG280 (VHG280Q) /CSM/VH/VHG2-D/VHG280Q.Seq.d/                       1693   0.0
VHP793 (VHP793Q) /CSM/VH/VHP7-D/VHP793Q.Seq.d/                       1025   0.0
VHP770 (VHP770Q) /CSM/VH/VHP7-C/VHP770Q.Seq.d/                       1025   0.0
SHJ792 (SHJ792Q) /CSM/SH/SHJ7-D/SHJ792Q.Seq.d/                       1025   0.0
VHN507 (VHN507Q) /CSM/VH/VHN5-A/VHN507Q.Seq.d/                       1011   0.0
SFL343 (SFL343Q) /CSM/SF/SFL3-B/SFL343Q.Seq.d/                       1003   0.0
VHF513 (VHF513Q) /CSM/VH/VHF5-A/VHF513Q.Seq.d/                        967   0.0
VHM645 (VHM645Q) /CSM/VH/VHM6-B/VHM645Q.Seq.d/                        928   0.0
VHP249 (VHP249Q) /CSM/VH/VHP2-C/VHP249Q.Seq.d/                        676   0.0
VHF409 (VHF409Q) /CSM/VH/VHF4-A/VHF409Q.Seq.d/                        676   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ440520) Dictyostelium discoideum cDNA clone:ddv43o20, 3' ...  1057   0.0    1
(BJ446486) Dictyostelium discoideum cDNA clone:ddv62j23, 3' ...   981   0.0    2
(BJ446419) Dictyostelium discoideum cDNA clone:ddv62l17, 3' ...   981   0.0    2
(BJ407847) Dictyostelium discoideum cDNA clone:dds43h23, 3' ...   981   0.0    2
(BJ445014) Dictyostelium discoideum cDNA clone:ddv58m01, 3' ...   967   0.0    2
(BJ401458) Dictyostelium discoideum cDNA clone:dds23f11, 3' ...   975   0.0    3
(BJ439930) Dictyostelium discoideum cDNA clone:ddv42i03, 3' ...   936   0.0    2
(BJ444433) Dictyostelium discoideum cDNA clone:ddv56i12, 3' ...   912   0.0    1
(BJ421821) Dictyostelium discoideum cDNA clone:ddv43o20, 5' ...   420   e-140  3
(BJ367847) Dictyostelium discoideum cDNA clone:ddc44c13, 5' ...   420   e-140  3
dna update 2008.11. 2
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54KM7) RecName: Full=Glycine dehydrogenase [decarboxylating], ...   343   6e-93
AK226865_1(AK226865|pid:none) Arabidopsis thaliana mRNA for puta...   232   2e-59
(O80988) RecName: Full=Glycine dehydrogenase [decarboxylating], ...   232   2e-59
(Q94B78) RecName: Full=Putative glycine dehydrogenase [decarboxy...   227   5e-58
CP000828_5806(CP000828|pid:none) Acaryochloris marina MBIC11017,...   227   6e-58
GN098317_1(GN098317|pid:none) Sequence 3098 from Patent WO200903...   224   4e-57
AY065004_1(AY065004|pid:none) Arabidopsis thaliana AT4g33010/F26...   224   4e-57
Z25857_1(Z25857|pid:none) F.pringlei mRNA for P subunit of glyci...   223   9e-57
(P49362) RecName: Full=Glycine dehydrogenase [decarboxylating] B...   223   1e-56
(O49850) RecName: Full=Glycine dehydrogenase [decarboxylating], ...   223   1e-56
protein update 2009. 7.11
PSORT 

 psg: 0.56  gvh: 0.22  alm: 0.48  top: 0.53  tms: 0.00  mit: 0.39  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 20.0 %: mitochondrial
	 16.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHG280 is nuc
5' end seq. ID VHG280F
5' end seq. 
>VHG280F.Seq
AACTCGTTCACTTATTTTGTTGTTCGATAATATTATTTTATTTATTTATTTTATTATAAC
TATTTTTGTTGTTGGATTGTTGTAATATTTATTTTAAATAATAAATATAAATAATAAAAT
AAAAATGTTAAAATTATTACGTAATAATGGAATAAATAAATTAAAATCTAATTTAATTAG
AAATTATTCAACAAAACAATCAATATTTCAAGCATTAGATACATTTCCAAAAAGACATAT
TGGACCAAATGAAAATGAAATTAATGAAATGTTAAAAAGTATTAATACATCAAAACTTTC
AAAAAAGAATCCAAATAGTTTAGAACAATTGATCGAATATACAATTCCAAAGGATATTAG
ATTAAATAGAGAATTAAATATTGAAGAGAATAAAGTTATTGGTGAGAATCAATTATTAAA
GGATTTAAAAAAGATTGCAGAAAAGAATAAAGTTTATAGATCATTCATTGGCATGGGTTA
TTATGGAACAATTACACCACATGTAATTCAACGTAACATTTTAGAGANNNNNNNNNN
Length of 5' end seq. 537
3' end seq. ID VHG280Z
3' end seq. 
>VHG280Z.Seq
NNNNNNNNNNAGCAACCCAAGTCGCCATTCTCAATGCAAACTATATGGCAAGTAGATTAA
AGGATCACTATAAAATTCTTTACACTGGTTCCCATGGTTTGGTAGCTCATGAATTCATTA
TTGATCTTAGAATGTTTAAGGAATCTGCTGGTATCGAAGCTGAAGATGTAGCTAAACGTC
TTCAAGATATGAATTTCCATGGTCCAACCATGTCTTGGCCAGTACCAAATACTCTTATGA
TTGAACCAACCGAATCTGAATCAAAATATGAATTGGATCGTCTTTGTGATGCTCTCATTT
TAATTCGTGAGGAAATTAGAGAGATTGAAACTGGTAAAGCCGACAGAAAAAATAATGTTC
TCGTTAATTCTCCACACACTGAAAAAGTTATTGTCGCTGATAATTGGAATTATCCATACT
CTCGTTCTAAAGCTGCTTTCCCAACTCCTGCAACTGTTGCCTCAAAATTCTGGCCAACCG
TTGGTAGAATTGATAACGTTCATGGCGATAAAAATTTAGTTTGTTCNTGCCCACCACTTT
CNCATTATCAATAAAATAAAATAAAAAATTAAATAAATA
Length of 3' end seq. 579
Connected seq. ID VHG280P
Connected seq. 
>VHG280P.Seq
AACTCGTTCACTTATTTTGTTGTTCGATAATATTATTTTATTTATTTATTTTATTATAAC
TATTTTTGTTGTTGGATTGTTGTAATATTTATTTTAAATAATAAATATAAATAATAAAAT
AAAAATGTTAAAATTATTACGTAATAATGGAATAAATAAATTAAAATCTAATTTAATTAG
AAATTATTCAACAAAACAATCAATATTTCAAGCATTAGATACATTTCCAAAAAGACATAT
TGGACCAAATGAAAATGAAATTAATGAAATGTTAAAAAGTATTAATACATCAAAACTTTC
AAAAAAGAATCCAAATAGTTTAGAACAATTGATCGAATATACAATTCCAAAGGATATTAG
ATTAAATAGAGAATTAAATATTGAAGAGAATAAAGTTATTGGTGAGAATCAATTATTAAA
GGATTTAAAAAAGATTGCAGAAAAGAATAAAGTTTATAGATCATTCATTGGCATGGGTTA
TTATGGAACAATTACACCACATGTAATTCAACGTAACATTTTAGAGA----------AGC
AACCCAAGTCGCCATTCTCAATGCAAACTATATGGCAAGTAGATTAAAGGATCACTATAA
AATTCTTTACACTGGTTCCCATGGTTTGGTAGCTCATGAATTCATTATTGATCTTAGAAT
GTTTAAGGAATCTGCTGGTATCGAAGCTGAAGATGTAGCTAAACGTCTTCAAGATATGAA
TTTCCATGGTCCAACCATGTCTTGGCCAGTACCAAATACTCTTATGATTGAACCAACCGA
ATCTGAATCAAAATATGAATTGGATCGTCTTTGTGATGCTCTCATTTTAATTCGTGAGGA
AATTAGAGAGATTGAAACTGGTAAAGCCGACAGAAAAAATAATGTTCTCGTTAATTCTCC
ACACACTGAAAAAGTTATTGTCGCTGATAATTGGAATTATCCATACTCTCGTTCTAAAGC
TGCTTTCCCAACTCCTGCAACTGTTGCCTCAAAATTCTGGCCAACCGTTGGTAGAATTGA
TAACGTTCATGGCGATAAAAATTTAGTTTGTTCNTGCCCACCACTTTCNCATTATCAATA
AAATAAAATAAAAAATTAAATAAATA
Length of connected seq. 1096
Full length Seq ID -
Full length Seq. -
Length of full length seq. -