NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHG250
Library VH
(Link to library)
Clone ID VHG250
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14496-1
Original site URL
Representative seq. ID
(Link to Original site)
Representative DNA sequence 
>VHG250 (VHG250Q) /CSM/VH/VHG2-C/VHG250Q.Seq.d/
AGTCAGTATCACATATTAAAATGCCAACCAGATTCAGTAAGCATAGAAAATCAAGAGGTG
ACGTTTGTGCCGGTTACGGTCGTGTTGGTAAACACAGAAAGCATCCAGGTGGTCGTGGTA
ATGCTGGTGGTCTCACTCATCACAGAATTAACTTTGACAAATACCATCCAGGTTATTTCG
GTAAATTAGGTATGAGACATTTCCATTTATTAAGAAACCAATACCATTGTCCAACCGTCT
CATTAGAAAAGATCTGGACTTTAGTCCCAGAATCCGTCAGAAAATCATTAGCTGCCAAGA
ATGACGGTACTGCTCCAGTTGTTGATGTCACCCAAAAAGGTTTCTTCAAAGTTTTAGGTC
ACGGTATCCTCCCAACTCAACCAATCATCGTCAAAGCTCGTTATTTCTCTAAAGTCGCTG
AAAAGAAAATCAAAGCTGTCGGTGGTGCTTGTATCTTAGTTGCTTAAATATTTTATACGA
ATCAATAAATATAAATAAAAAAAGTAATTAAATTTAAAAAATATNNNNNGAANAAAAAAX
XXXXXXXXX
sequence update 2002.10.25
Translated Amino Acid sequence 
SVSHIKMPTRFSKHRKSRGDVCAGYGRVGKHRKHPGGRGNAGGLTHHRINFDKYHPGYFG
KLGMRHFHLLRNQYHCPTVSLEKIWTLVPESVRKSLAAKNDGTAPVVDVTQKGFFKVLGH
GILPTQPIIVKARYFSKVAEKKIKAVGGACILVA*ifytnq*i*ikkvikfkkyxxxk--
-
Translated Amino Acid sequence (All Frames) 
Frame A:
sqyhilkcqpdsvsienqevtfvpvtvvlvntesiqvvvvmlvvsliteltltntiqvis
vn*v*disiy*etntivqpsh*krsgl*sqnpsenh*lprmtvllqllmspkkvsskf*v
tvssqlnqsssklvislkslkrksklsvvlvs*llkyfirinkyk*kk*lnlknxxxxk-
--


Frame B:
vsity*nanqiq*a*kikr*rlcrlrscw*tqkasrwsw*cwwshssqn*l*qipsrlfr
*iryetfpfikkpiplsnrlirkdldfsprirqkiiscqe*rycssc*chpkrflqsfrs
ryppnstnhrqsslfl*sr*kenqscrwclylsclnilyesininkksn*i*kixxexk-
--


Frame C:
SVSHIKMPTRFSKHRKSRGDVCAGYGRVGKHRKHPGGRGNAGGLTHHRINFDKYHPGYFG
KLGMRHFHLLRNQYHCPTVSLEKIWTLVPESVRKSLAAKNDGTAPVVDVTQKGFFKVLGH
GILPTQPIIVKARYFSKVAEKKIKAVGGACILVA*ifytnq*i*ikkvikfkkyxxxk--
-
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHO146 (VHO146Q) /CSM/VH/VHO1-B/VHO146Q.Seq.d/                        957   0.0
VHG250 (VHG250Q) /CSM/VH/VHG2-C/VHG250Q.Seq.d/                        957   0.0
VFF473 (VFF473Q) /CSM/VF/VFF4-D/VFF473Q.Seq.d/                        957   0.0
VFE287 (VFE287Q) /CSM/VF/VFE2-D/VFE287Q.Seq.d/                        957   0.0
VSJ737 (VSJ737Q) /CSM/VS/VSJ7-B/VSJ737Q.Seq.d/                        950   0.0
VHE734 (VHE734Q) /CSM/VH/VHE7-B/VHE734Q.Seq.d/                        950   0.0
VSI571 (VSI571Q) /CSM/VS/VSI5-C/VSI571Q.Seq.d/                        944   0.0
VSE523 (VSE523Q) /CSM/VS/VSE5-A/VSE523Q.Seq.d/                        944   0.0
VSA537 (VSA537Q) /CSM/VS/VSA5-B/VSA537Q.Seq.d/                        944   0.0
VSH826 (VSH826Q) /CSM/VS/VSH8-B/VSH826Q.Seq.d/                        940   0.0
own update 2002.12.27
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ445414) Dictyostelium discoideum cDNA clone:ddv59k11, 3' ...   957   0.0    1
(BJ431114) Dictyostelium discoideum cDNA clone:ddv9m22, 3' e...   957   0.0    1
(BJ428387) Dictyostelium discoideum cDNA clone:ddv11b20, 3' ...   957   0.0    1
(BJ421687) Dictyostelium discoideum cDNA clone:ddv43c14, 5' ...   957   0.0    1
(BJ412802) Dictyostelium discoideum cDNA clone:ddv9m22, 5' e...   957   0.0    1
(BJ410234) Dictyostelium discoideum cDNA clone:ddv11b20, 5' ...   957   0.0    1
(AU270392) Dictyostelium discoideum vegetative cDNA clone:VS...   950   0.0    1
(BJ420740) Dictyostelium discoideum cDNA clone:ddv40d09, 5' ...   950   0.0    1
(AU268909) Dictyostelium discoideum vegetative cDNA clone:VS...   944   0.0    1
(AU263167) Dictyostelium discoideum vegetative cDNA clone:VS...   944   0.0    1
dna update 2008.11. 1
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P48160) RecName: Full=60S ribosomal protein L27a;  &D50338_1(D5...   313   2e-84
BT046766_1(BT046766|pid:none) Salmo salar clone ssal-eve-548-138...   226   2e-58
AY961477_1(AY961477|pid:none) Phytophthora infestans clone MY-19...   226   4e-58
AF502247_1(AF502247|pid:none) Epinephelus coioides ribosomal pro...   223   3e-57
AJ843085_1(AJ843085|pid:none) Platichthys flesus mRNA for 60S ri...   223   3e-57
(P18445) RecName: Full=60S ribosomal protein L27a;  &R5RTLA(S130...   222   4e-57
AB374966_1(AB374966|pid:none) Solea senegalensis RPL27a mRNA for...   221   7e-57
(P46776) RecName: Full=60S ribosomal protein L27a;  &(Q5REY2) Re...   221   7e-57
AJ307670_3(AJ307670|pid:none) Mus musculus St5 gene, L27a gene a...   221   7e-57
AB172987_1(AB172987|pid:none) Macaca fascicularis brain cDNA clo...   220   2e-56
protein update 2009. 7.11
PSORT 

 psg: 0.34  gvh: 0.18  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.40  mip: 0.12
 nuc: 0.05  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.50  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: cytoplasmic
	 20.0 %: mitochondrial
	 16.0 %: nuclear
	  4.0 %: plasma membrane

>> prediction for VHG250 is cyt
5' end seq. ID VHG250F
5' end seq. 
>VHG250F.Seq
AGTCAGTATCACATATTAAAATGCCAACCAGATTCAGTAAGCATAGAAAATCAAGAGGTG
ACGTTTGTGCCGGTTACGGTCGTGTTGGTAAACACAGAAAGCATCCAGGTGGTCGTGGTA
ATGCTGGTGGTCTCACTCATCACAGAATTAACTTTGACAAATACCATCCAGGTTATTTCG
GTAAATTAGGTATGAGACATTTCCATTTATTAAGAAACCAATACCATTGTCCAACCGTCT
CATTAGAAAAGATCTGGACTTTAGTCCCAGAATCCGTCAGAAAATCATTAGCTGCCAAGA
ATGACGGTACTGCTCCAGTTGTTGATGTCACCCAAAAAGGTTTCTTCAAAGTTTTAGGTC
ACGGTATCCTCCCAACTCAACCAATCATCGTCAAAGCTCGTTATTTCTCTAAAGTCGCTG
AAAAGAAAATCAAAGCTGTCGGTGGTGCTTGTATCTTAGTTGCTTAAATATTTTATACGA
ATCAATAAATATAAATAAAAAAAGTAATTAAATTTAAAAAATATNNNNNGAANAAAAAAN
NNNNNNNNN
Length of 5' end seq. 549
3' end seq. ID -
3' end seq. -
Length of 3' end seq. -
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -