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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHG223
Library VH
(Link to library)
Clone ID VHG223
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U13945-1
Original site URL
Representative seq. ID VHG223P
(Link to Original site)
Representative DNA sequence 
>VHG223 (VHG223Q) /CSM/VH/VHG2-A/VHG223Q.Seq.d/
AAAAAAAATNATTTACTCATACTTTAATTTATAATTTGCTTTTTTCTTATATATTTCATA
TATTTATTATTTATTTATTTATTTATTTTTTTTACAAAGGGGAAAACAAAGATATTGGTG
TTTGGTCTTTCTATTGTTTTTCTTTGTAATAAATAACCTCAAACACCCACACACAAAAAC
AAAAATAAACACAATGAAATCCATACCAATAATTTTATTAGTATTGATTGGATTATTATT
AGCATCTGTATATTCCCATGACATTTTAATTACAGAAAAAGGACAAAAATCATTAGATAA
ATTAGATAAAGCAGCAGCAACAACTTTACAACATGGTGAAAACATTCAATTAAGCTGTGA
TGTTTGTCAAATTGGTGCATCATTATTAGAAGATTTCATTAAAAAGAATGCTTCATTAAC
AGAAATAATTAAAGGTTTATCTGACCTTTGTATTGCCTCAAAAGAAGAACAACCAGAAGT
TTGTACAGGTATTTTAAATAATTATGTACCAATTATAGTTGATGTACTTATTCAATCAGA
TTTTACACCATCACAATTATGTGGATATTTTAAGATTTGTTCAGCAACAGGATCATCAGA
ATCATCATCAATTTCAAATTCATTTGATAATGAATATAGAXXXXXXXXXXTATTTAAAAG
GCCCAAATAATCAATCGGATTGGATGATCAATACATTGGAACAAGCTCAATCCAATGGTG
AAAAGGTTTTAATCATTGGTCATATTCCATGCACTGTTAAAAGTGCTTCAACCGATGGTT
GGTGTGCAATGTATGAACAAGTGGTAGGTCAATTCTCTGATGTTATCATTGGTCAATTGT
ATGGCCATACTCATTACGATCAATTTTCAGTATTCTCCGATGTTGCAACTCATACAATTC
CAACCGGTATGAATTATATCGTTCCATCATTAACAACCTATCAAAATCATGAACCTGGTT
ATCGTATTTATCAATTTGATTATTCAACTAATCAAATTGTAAACTACTATCAATATCATG
CAAACATTACTGAAGCAAATGAAACTGGTGCCTTAAATTTCCAATTGACTTATAGTGCTA
AAGAACTCTATAATATGGATGATCTTTCACCTACCTCTTGGACAAAAGTTGCCAATCAAA
TGAAAACTAATTCAACAATGTTTAATAGTTATTTCGAAAATCTTAGTAGTTCACCAATTA
AAGAATCTTGTGATCAAGCTTGTCAAACTAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
kkxiysyfnl*FAFFLYISYIYYLFIYLFFLQRGKQRYWCLVFLLFFFVINNLKHPHTKT
KINTMKSIPIILLVLIGLLLASVYSHDILITEKGQKSLDKLDKAAATTLQHGENIQLSCD
VCQIGASLLEDFIKKNASLTEIIKGLSDLCIASKEEQPEVCTGILNNYVPIIVDVLIQSD
FTPSQLCGYFKICSATGSSESSSISNSFDNEYR---

---YLKGPNNQSDWMINTLEQAQSNGEKVLIIGHIPCTVKSASTDGWCAMYEQVVGQFSD
VIIGQLYGHTHYDQFSVFSDVATHTIPTGMNYIVPSLTTYQNHEPGYRIYQFDYSTNQIV
NYYQYHANITEANETGALNFQLTYSAKELYNMDDLSPTSWTKVANQMKTNSTMFNSYFEN
LSSSPIKESCDQACQTK
Translated Amino Acid sequence (All Frames) 
Frame A:
kknxllil*fiicffliyfiyllfiylfifftkgktkilvfglsivflcnk*pqtpthkn
knkhneihtnnfisidwiiisicifp*hfnyrkrtkiir*ir*sssnnfttw*khsikl*
clsnwciiirrfh*kecfinrnn*rfi*plyclkrrttrslyryfk*lctnys*ctysir
fytitimwif*dlfsnriiriiinfkfi***i*---

---YLKGPNNQSDWMINTLEQAQSNGEKVLIIGHIPCTVKSASTDGWCAMYEQVVGQFSD
VIIGQLYGHTHYDQFSVFSDVATHTIPTGMNYIVPSLTTYQNHEPGYRIYQFDYSTNQIV
NYYQYHANITEANETGALNFQLTYSAKELYNMDDLSPTSWTKVANQMKTNSTMFNSYFEN
LSSSPIKESCDQACQTK

Frame B:
kkxiysyfnl*FAFFLYISYIYYLFIYLFFLQRGKQRYWCLVFLLFFFVINNLKHPHTKT
KINTMKSIPIILLVLIGLLLASVYSHDILITEKGQKSLDKLDKAAATTLQHGENIQLSCD
VCQIGASLLEDFIKKNASLTEIIKGLSDLCIASKEEQPEVCTGILNNYVPIIVDVLIQSD
FTPSQLCGYFKICSATGSSESSSISNSFDNEYR---

---i*kaqiinrig*sihwnklnpmvkrf*slvifhallkvlqpmvgvqcmnkw*vnslm
lslvncmailitinfqyspmlqliqfqpv*iisfhh*qpikimnlvivfinliiqlikl*
ttinimqtllkqmklvp*isn*livlknsiiwmifhlplgqklpik*kliqqcliviski
lvvhqlknlviklvkl

Frame C:
kkxfthtliynllfsyifhifiiylfiyffykgenkdigvwsfycfsl**itsnthtqkq
k*tq*npyq*fy*y*ldyy*hlyipmtf*lqkkdknh*in*ikqqqqlynmvktfn*avm
fvklvhhy*kislkrmlh*qk*lkvyltfvlpqkknnqkfvqvf*iimyql*lmylfnqi
lhhhnyvdilrfvqqqdhqnhhqfqihlimni---

---fkrpk*siglddqyigtssiqw*kgfnhwsysmhc*kcfnrwlvcnv*tsgrsil*c
yhwsivwpyslrsifsilrccnsynsnryelyrsiinnlsks*twlsylsi*lfn*snck
llsisckhy*sk*nwclkfpidl*c*rtl*yg*sftylldkscqsnen*fnnv**lfrks
**ftn*ril*sslsn*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHG223 (VHG223Q) /CSM/VH/VHG2-A/VHG223Q.Seq.d/                       1998   0.0
VHB601 (VHB601Q) /CSM/VH/VHB6-A/VHB601Q.Seq.d/                       1154   0.0
VFL234 (VFL234Q) /CSM/VF/VFL2-B/VFL234Q.Seq.d/                       1146   0.0
VHC519 (VHC519Q) /CSM/VH/VHC5-A/VHC519Q.Seq.d/                       1102   0.0
VHJ154 (VHJ154Q) /CSM/VH/VHJ1-C/VHJ154Q.Seq.d/                       1088   0.0
VFH473 (VFH473Q) /CSM/VF/VFH4-D/VFH473Q.Seq.d/                       1009   0.0
AFI211 (AFI211Q) /CSM/AF/AFI2-A/AFI211Q.Seq.d/                        884   0.0
AFN745 (AFN745Q) /CSM/AF/AFN7-B/AFN745Q.Seq.d/                        739   0.0
VFH433 (VFH433Q) /CSM/VF/VFH4-B/VFH433Q.Seq.d/                        567   e-160
VHG422 (VHG422Q) /CSM/VH/VHG4-A/VHG422Q.Seq.d/                        329   6e-89
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ440270) Dictyostelium discoideum cDNA clone:ddv43m06, 3' ...  1154   0.0    1
(BJ437348) Dictyostelium discoideum cDNA clone:ddv34a02, 3' ...  1154   0.0    1
(BJ433894) Dictyostelium discoideum cDNA clone:ddv23c10, 3' ...  1146   0.0    1
(BJ438002) Dictyostelium discoideum cDNA clone:ddv36e05, 3' ...  1102   0.0    1
(BJ442360) Dictyostelium discoideum cDNA clone:ddv49k13, 3' ...  1086   0.0    2
(BJ431835) Dictyostelium discoideum cDNA clone:ddv15b20, 3' ...   977   0.0    2
(BJ421587) Dictyostelium discoideum cDNA clone:ddv43m06, 5' ...   884   0.0    2
(BJ419418) Dictyostelium discoideum cDNA clone:ddv36e05, 5' ...   884   0.0    2
(BJ326578) Dictyostelium discoideum cDNA clone:dda17e04, 5' ...   884   0.0    2
(BJ328487) Dictyostelium discoideum cDNA clone:dda28j11, 5' ...   500   0.0    3
dna update 2008.11. 1
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q0VD19) RecName: Full=Sphingomyelin phosphodiesterase;         ...   128   4e-28
AY261860_1(AY261860|pid:none) Ciona intestinalis sphingomyelin p...   126   2e-27
A39825(S27009;S36357;A39825;JX0276;S06957;I55567;A42801;) sphing...   125   2e-27
X63600_1(X63600|pid:none) H.sapiens genes for acid sphingomyelin...   125   2e-27
AK292388_1(AK292388|pid:none) Homo sapiens cDNA FLJ77600 complet...   125   2e-27
X59960_1(X59960|pid:none) H.sapiens mRNA for sphingomyelinase.        125   3e-27
AE013599_4075(AE013599|pid:none) Drosophila melanogaster chromos...   125   3e-27
(P17405) RecName: Full=Sphingomyelin phosphodiesterase;         ...   125   3e-27
AE013599_4074(AE013599|pid:none) Drosophila melanogaster chromos...   125   3e-27
M81780_2(M81780|pid:none) Homo sapiens acid sphingomyelinase (SM...   125   3e-27
protein update 2009. 7.11
PSORT 

 psg: 0.98  gvh: 0.53  alm: 0.17  top: 0.67  tms: 0.27  mit: 0.36  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 1.00  m_ : 0.00 

	 40.0 %: plasma membrane
	 20.0 %: endoplasmic reticulum
	 12.0 %: mitochondrial
	 12.0 %: vacuolar
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: nuclear
	  4.0 %: cytoplasmic

>> prediction for VHG223 is pla
5' end seq. ID VHG223F
5' end seq. 
>VHG223F.Seq
AAAAAAAATNATTTACTCATACTTTAATTTATAATTTGCTTTTTTCTTATATATTTCATA
TATTTATTATTTATTTATTTATTTATTTTTTTTACAAAGGGGAAAACAAAGATATTGGTG
TTTGGTCTTTCTATTGTTTTTCTTTGTAATAAATAACCTCAAACACCCACACACAAAAAC
AAAAATAAACACAATGAAATCCATACCAATAATTTTATTAGTATTGATTGGATTATTATT
AGCATCTGTATATTCCCATGACATTTTAATTACAGAAAAAGGACAAAAATCATTAGATAA
ATTAGATAAAGCAGCAGCAACAACTTTACAACATGGTGAAAACATTCAATTAAGCTGTGA
TGTTTGTCAAATTGGTGCATCATTATTAGAAGATTTCATTAAAAAGAATGCTTCATTAAC
AGAAATAATTAAAGGTTTATCTGACCTTTGTATTGCCTCAAAAGAAGAACAACCAGAAGT
TTGTACAGGTATTTTAAATAATTATGTACCAATTATAGTTGATGTACTTATTCAATCAGA
TTTTACACCATCACAATTATGTGGATATTTTAAGATTTGTTCAGCAACAGGATCATCAGA
ATCATCATCAATTTCAAATTCATTTGATAATGAATATAGANNNNNNNNNN
Length of 5' end seq. 650
3' end seq. ID VHG223Z
3' end seq. 
>VHG223Z.Seq
NNNNNNNNNNTATTTAAAAGGCCCAAATAATCAATCGGATTGGATGATCAATACATTGGA
ACAAGCTCAATCCAATGGTGAAAAGGTTTTAATCATTGGTCATATTCCATGCACTGTTAA
AAGTGCTTCAACCGATGGTTGGTGTGCAATGTATGAACAAGTGGTAGGTCAATTCTCTGA
TGTTATCATTGGTCAATTGTATGGCCATACTCATTACGATCAATTTTCAGTATTCTCCGA
TGTTGCAACTCATACAATTCCAACCGGTATGAATTATATCGTTCCATCATTAACAACCTA
TCAAAATCATGAACCTGGTTATCGTATTTATCAATTTGATTATTCAACTAATCAAATTGT
AAACTACTATCAATATCATGCAAACATTACTGAAGCAAATGAAACTGGTGCCTTAAATTT
CCAATTGACTTATAGTGCTAAAGAACTCTATAATATGGATGATCTTTCACCTACCTCTTG
GACAAAAGTTGCCAATCAAATGAAAACTAATTCAACAATGTTTAATAGTTATTTCGAAAA
TCTTAGTAGTTCACCAATTAAAGAATCTTGTGATCAAGCTTGTCAAACTAAA
Length of 3' end seq. 592
Connected seq. ID VHG223P
Connected seq. 
>VHG223P.Seq
AAAAAAAATNATTTACTCATACTTTAATTTATAATTTGCTTTTTTCTTATATATTTCATA
TATTTATTATTTATTTATTTATTTATTTTTTTTACAAAGGGGAAAACAAAGATATTGGTG
TTTGGTCTTTCTATTGTTTTTCTTTGTAATAAATAACCTCAAACACCCACACACAAAAAC
AAAAATAAACACAATGAAATCCATACCAATAATTTTATTAGTATTGATTGGATTATTATT
AGCATCTGTATATTCCCATGACATTTTAATTACAGAAAAAGGACAAAAATCATTAGATAA
ATTAGATAAAGCAGCAGCAACAACTTTACAACATGGTGAAAACATTCAATTAAGCTGTGA
TGTTTGTCAAATTGGTGCATCATTATTAGAAGATTTCATTAAAAAGAATGCTTCATTAAC
AGAAATAATTAAAGGTTTATCTGACCTTTGTATTGCCTCAAAAGAAGAACAACCAGAAGT
TTGTACAGGTATTTTAAATAATTATGTACCAATTATAGTTGATGTACTTATTCAATCAGA
TTTTACACCATCACAATTATGTGGATATTTTAAGATTTGTTCAGCAACAGGATCATCAGA
ATCATCATCAATTTCAAATTCATTTGATAATGAATATAGA----------TATTTAAAAG
GCCCAAATAATCAATCGGATTGGATGATCAATACATTGGAACAAGCTCAATCCAATGGTG
AAAAGGTTTTAATCATTGGTCATATTCCATGCACTGTTAAAAGTGCTTCAACCGATGGTT
GGTGTGCAATGTATGAACAAGTGGTAGGTCAATTCTCTGATGTTATCATTGGTCAATTGT
ATGGCCATACTCATTACGATCAATTTTCAGTATTCTCCGATGTTGCAACTCATACAATTC
CAACCGGTATGAATTATATCGTTCCATCATTAACAACCTATCAAAATCATGAACCTGGTT
ATCGTATTTATCAATTTGATTATTCAACTAATCAAATTGTAAACTACTATCAATATCATG
CAAACATTACTGAAGCAAATGAAACTGGTGCCTTAAATTTCCAATTGACTTATAGTGCTA
AAGAACTCTATAATATGGATGATCTTTCACCTACCTCTTGGACAAAAGTTGCCAATCAAA
TGAAAACTAATTCAACAATGTTTAATAGTTATTTCGAAAATCTTAGTAGTTCACCAATTA
AAGAATCTTGTGATCAAGCTTGTCAAACTAAA
Length of connected seq. 1222
Full length Seq ID -
Full length Seq. -
Length of full length seq. -