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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFN855
Library AF
(Link to library)
Clone ID AFN855
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10734-1
Original site URL
Representative seq. ID AFN855Z
(Link to Original site)
Representative DNA sequence 
>AFN855 (AFN855Q) /CSM/AF/AFN8-C/AFN855Q.Seq.d/
XXXXXXXXXXCAAAAAGTATGAATCAATCGCCGTTCCATTAGCTTATTTATTCATTGACT
CCACTCAAGATAACACTCAAGTTCTCGAAGATGTAAAGAAAATCGCTACCTCACAAAAAG
GTAATGCCGTTTTCTGTTGGGTCGATATGAAGAAATTCCCACAACAAGCAACTCATATGG
GTCTCTCTGGTAAAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTT
ACAATTTCGATGAAAAAGAAACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGATG
TCATTGGTGGTAAAGTTTCTCCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGATG
CTCCAGTTAAGGTTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGG
ATGTTTTGGTTGAATTCTACGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCT
ACGATAAATTAGGTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATG
CCGACTCAAATGATGTACCAAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTCT
TCAAAGCTGATGACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGAAATGATCATATGA
ACTTTGTTGAATTCATTCAAGATAACGCTGCCATTGAATTCAAATTACCATCATCTCAAA
CTGATGATAATGTGAATCTAAAAAAGATTCTTCTGCTAAACATGANAATTATAAATAAAT
TTAATAAATATATAATAATATTTTAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
---KKYESIAVPLAYLFIDSTQDNTQVLEDVKKIATSQKGNAVFCWVDMKKFPQQATHMG
LSGKVVPAISVDSVANKARYNFDEKETFSFDTVSKWIQDVIGGKVSPFVKSQPEPESNDA
PVKVAVGTTFKKLVLDSPKDVLVEFYAPWCGHCKNLAPIYDKLGEYLKDVESVSIVKIDA
DSNDVPSDIEIRGYPTIMLFKADDKENPISYEGQRNDHMNFVEFIQDNAAIEFKLPSSQT
DDNVNLKKILLLNMXIINKFNKYIIIF*
Translated Amino Acid sequence (All Frames) 
Frame A:
---KKYESIAVPLAYLFIDSTQDNTQVLEDVKKIATSQKGNAVFCWVDMKKFPQQATHMG
LSGKVVPAISVDSVANKARYNFDEKETFSFDTVSKWIQDVIGGKVSPFVKSQPEPESNDA
PVKVAVGTTFKKLVLDSPKDVLVEFYAPWCGHCKNLAPIYDKLGEYLKDVESVSIVKIDA
DSNDVPSDIEIRGYPTIMLFKADDKENPISYEGQRNDHMNFVEFIQDNAAIEFKLPSSQT
DDNVNLKKILLLNMXIINKFNKYIIIF*


Frame B:
---ksmnqspfh*liysltplkitlkfskm*rkslphkkvmpfsvgsi*rnshnkqliwv
slvklfqpslliqlpiklvtismkkkpshsipspngskmslvvkflhllnlnqnqnqmml
qlrlplvplskswf*thqrmfwlnstlhgaviarillqstin*vnt*ktlnlsqs*rlmp
tqmmyqvisklevtqpscssklmtkkiqslmkvkemii*tllnsfkitlplnsnyhhlkl
mim*i*krffc*t*xl*inlini**yf


Frame C:
---kv*inrrsislfih*lhsr*hsssrrckenryltkr*crfllgryeeipttsnsygs
lw*scsshlc*fscq*sslqfr*krnlliryrlqmdprchww*sfsic*istrtrik*cs
s*gcrwyhfqkvgfrltkgcfg*ilrsmvrslqescsnlr*ir*ilkrr*iclnrkd*cr
lk*ctk*yrn*rlpnhhalqs**qrksnll*rskk*syelc*ihsr*rch*iqitiisn*
**ceskkdssakhxnyk*i**iynnil
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFN855 (AFN855Q) /CSM/AF/AFN8-C/AFN855Q.Seq.d/                       1497   0.0
AFK269 (AFK269Q) /CSM/AF/AFK2-C/AFK269Q.Seq.d/                       1459   0.0
AFL563 (AFL563Q) /CSM/AF/AFL5-C/AFL563Q.Seq.d/                       1439   0.0
AFO368 (AFO368Q) /CSM/AF/AFO3-C/AFO368Q.Seq.d/                       1437   0.0
AFO474 (AFO474Q) /CSM/AF/AFO4-D/AFO474Q.Seq.d/                       1433   0.0
AFK148 (AFK148Q) /CSM/AF/AFK1-B/AFK148Q.Seq.d/                       1429   0.0
AFM464 (AFM464Q) /CSM/AF/AFM4-C/AFM464Q.Seq.d/                       1415   0.0
AFK889 (AFK889Q) /CSM/AF/AFK8-D/AFK889Q.Seq.d/                       1413   0.0
AFO686 (AFO686Q) /CSM/AF/AFO6-D/AFO686Q.Seq.d/                       1411   0.0
AFN679 (AFN679Q) /CSM/AF/AFN6-D/AFN679Q.Seq.d/                       1411   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BJ171852|BJ171852.1 Physcomitrella patens subsp. patens cDNA clone:pph30f12, 3' end, single read.   599  e-167  1
BM398179|BM398179.1 5009-0-41-G11.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    74  3e-09  1
AL924030|AL924030.1 Danio rerio EST sequence, clone 134-B07-2.    70  5e-08  1
CB176061|CB176061.1 pl17e07.y3 Ancylostoma ceylanicum adult Ancylostoma ceylanicum cDNA 5' similar to TR:O17908 O17908 H06O01.1 PROTEIN. [1] ;, mRNA sequence.    70  5e-08  1
BM280519|BM280519.1 ki01h03.y1 Ascaris suum L4 pSPORT1 Zarlenga v1 Ascaris suum cDNA 5' similar to SW:ERP5_CAEEL Q11067 PROBABLE PROTEIN DISULFIDE ISOMERASE P5 PRECURSOR ;, mRNA sequence.    68  2e-07  1
AW255071|AW255071.1 ML1365 peppermint glandular trichome Mentha x piperita cDNA, mRNA sequence.    64  3e-06  1
BM315730|BM315730.1 fw64d09.y1 Sugano SJD adult male Danio rerio cDNA clone 5612584 5' similar to SW:ER60_HUMAN P30101 PROBABLE PROTEIN DISULFIDE ISOMERASE ER-60 PRECURSOR ;, mRNA sequence.    62  1e-05  1
BM308748|BM308748.1 sak49h10.y1 Gm-c1036 Glycine max cDNA clone SOYBEAN CLONE ID: Gm-c1036-6523 5' similar to TR:P93358 P93358 PROTEIN DISULFIDE-ISOMERASE PRECURSOR. ;, mRNA sequence.    60  4e-05  1
BU795021|S34275 protein disulfide-isomerase homolog precursor - fluke  (Schistosoma mansoni), mRNA sequence.    60  4e-05  1
BM174369|PDA4_MOUSE PROTEIN DISULFIDE ISOMERASE A4 PRECURSOR (PROTEIN ERP-72) (ERP72), mRNA sequence.    60  4e-05  1
dna update 2003. 9.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q12730) RecName: Full=Protein disulfide-isomerase;          Sho...   188   1e-46
(Q00248) RecName: Full=Protein disulfide-isomerase;          Sho...   186   9e-46
CU633901_146(CU633901|pid:none) Podospora anserina genomic DNA c...   180   4e-44
EU016183_1(EU016183|pid:none) Haemaphysalis longicornis strain G...   174   3e-42
DQ066336_1(DQ066336|pid:none) Ixodes scapularis isolate IS-6-12-...   168   2e-40
DQ377176_1(DQ377176|pid:none) Amblyomma variegatum protein disul...   164   2e-39
CR382131_125(CR382131|pid:none) Yarrowia lipolytica strain CLIB1...   162   1e-38
(Q10057) RecName: Full=Putative protein disulfide-isomerase C1F5...   161   2e-38
U82634_1(U82634|pid:none) Alternaria alternata putative protein ...   156   8e-37
AE013599_1413(AE013599|pid:none) Drosophila melanogaster chromos...   154   2e-36
protein update 2009. 4.25
PSORT 

 psg: 0.92  gvh: 0.48  alm: 0.43  top: 0.27  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for AFN855 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID AFN855Z
3' end seq. 
>AFN855Z.Seq
----------CAAAAAGTATGAATCAATCGCCGTTCCATTAGCTTATTTATTCATTGACT
CCACTCAAGATAACACTCAAGTTCTCGAAGATGTAAAGAAAATCGCTACCTCACAAAAAG
GTAATGCCGTTTTCTGTTGGGTCGATATGAAGAAATTCCCACAACAAGCAACTCATATGG
GTCTCTCTGGTAAAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTT
ACAATTTCGATGAAAAAGAAACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGATG
TCATTGGTGGTAAAGTTTCTCCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGATG
CTCCAGTTAAGGTTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGG
ATGTTTTGGTTGAATTCTACGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCT
ACGATAAATTAGGTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATG
CCGACTCAAATGATGTACCAAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTCT
TCAAAGCTGATGACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGAAATGATCATATGA
ACTTTGTTGAATTCATTCAAGATAACGCTGCCATTGAATTCAAATTACCATCATCTCAAA
CTGATGATAATGTGAATCTAAAAAAGATTCTTCTGCTAAACATGANAATTATAAATAAAT
TTAATAAATATATAATAATATTTTAA
Length of 3' end seq. 796
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -