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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHF892
Library VH
(Link to library)
Clone ID VHF892
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VHF892P
(Link to Original site)
Representative DNA sequence 
>VHF892 (VHF892Q) /CSM/VH/VHF8-D/VHF892Q.Seq.d/
TCACCATTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAANGAAGACAAATTAC
CAGCTGGTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAAGTCACGTTG
ATTTCAGTTCXXXXXXXXXXATGAATTAACTGCNTCCCCAAATATCTTAGAACCAATCTA
CTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTANTCTGTANTCAATCG
TCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTATCTGTCAA
AGCTCATTTACCAGTATTAGAATCNTTCGGTTTCACCGATGATTTACGTTCCCACACTGC
CGGTCAAGNTTTCCCACAATGTGTCTTCGATCANTGGGCTTCAATTGGTGTTGTCAACAA
AGATAAGAAAGCCNCTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGA
AATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCATCTTTGAG
TTTTCAA
sequence update 2002.10.25
Translated Amino Acid sequence 
TIKSSSVSLHFEMPXEDKLPAGCTSHEFLINLIDSPSHVDFS---

---ELTASPNILEPIYLVEITAPENAIGGIXSVXNRRRGIVIGEERRIGSPLLSVKAHLP
VLESFGFTDDLRSHTAGQXFPQCVFDXWASIGVVNKDKKAXEVALATRKRKGLAPEIPDL
DKFHEKL*ti*vaslsfq
Translated Amino Acid sequence (All Frames) 
Frame A:
splnlhlyhftskcqxktnyqlvvlhtnf*silsipqvtlisv---

---mn*lxpqis*nqst**kllpqktllvvfxlxsivvevlllvknvvsvphyylskliy
qy*nxsvspmiyvptlpvkxshnvssixglqlvlstkirkplksl*pperekv*pqksqi
ltnstknskqfkshl*vf

Frame B:
hh*ificitslrnakxrqitswlyftrifnqsyrfpksr*fqf---

---*incxpkylrtnllsrnycprkrywwylxcxqss*rycyw*rtsyrfpiiicqssft
sirixrfhr*ftfphcrsxfptmclrsxgfnwccqqr*esx*srfshqkekrfsprnprs
*qiprktlnnlsrifefs

Frame C:
TIKSSSVSLHFEMPXEDKLPAGCTSHEFLINLIDSPSHVDFS---

---ELTASPNILEPIYLVEITAPENAIGGIXSVXNRRRGIVIGEERRIGSPLLSVKAHLP
VLESFGFTDDLRSHTAGQXFPQCVFDXWASIGVVNKDKKAXEVALATRKRKGLAPEIPDL
DKFHEKL*ti*vaslsfq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF892 (VHF892Q) /CSM/VH/VHF8-D/VHF892Q.Seq.d/                        977   0.0
VHF621 (VHF621Q) /CSM/VH/VHF6-A/VHF621Q.Seq.d/                        755   0.0
VHF768 (VHF768Q) /CSM/VH/VHF7-C/VHF768Q.Seq.d/                        753   0.0
VHF337 (VHF337Q) /CSM/VH/VHF3-B/VHF337Q.Seq.d/                        753   0.0
VHE892 (VHE892Q) /CSM/VH/VHE8-D/VHE892Q.Seq.d/                        753   0.0
VHF354 (VHF354Q) /CSM/VH/VHF3-C/VHF354Q.Seq.d/                        751   0.0
VHE647 (VHE647Q) /CSM/VH/VHE6-B/VHE647Q.Seq.d/                        747   0.0
VHE711 (VHE711Q) /CSM/VH/VHE7-A/VHE711Q.Seq.d/                        745   0.0
VHF895 (VHF895Q) /CSM/VH/VHF8-D/VHF895Q.Seq.d/                        743   0.0
VHF314 (VHF314Q) /CSM/VH/VHF3-A/VHF314Q.Seq.d/                        743   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(M26017) D.discoideum elongation factor 2 mRNA, complete cds.     617   0.0    4
(BJ440166) Dictyostelium discoideum cDNA clone:ddv42h24, 3' ...   765   0.0    1
(BJ439933) Dictyostelium discoideum cDNA clone:ddv42i06, 3' ...   755   0.0    1
(BJ440088) Dictyostelium discoideum cDNA clone:ddv42h17, 3' ...   753   0.0    1
(BJ439719) Dictyostelium discoideum cDNA clone:ddv41j09, 3' ...   753   0.0    1
(BJ439558) Dictyostelium discoideum cDNA clone:ddv40h24, 3' ...   753   0.0    1
(BJ439801) Dictyostelium discoideum cDNA clone:ddv41l13, 3' ...   751   0.0    1
(BJ439426) Dictyostelium discoideum cDNA clone:ddv40m12, 3' ...   747   0.0    1
(BJ439312) Dictyostelium discoideum cDNA clone:ddv40f03, 3' ...   745   0.0    1
(BJ440193) Dictyostelium discoideum cDNA clone:ddv42n24, 3' ...   743   0.0    1
dna update 2008.10.31
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q06193) RecName: Full=Elongation factor 2;          Short=EF-2;...   112   6e-24
AY831447_1(AY831447|pid:none) Monocercomonoides sp. PA203 transl...   105   8e-22
DQ848932_1(DQ848932|pid:none) Scophthalmus maximus clone kbc101 ...   105   8e-22
BC077595_1(BC077595|pid:none) Xenopus laevis translation elongat...   105   8e-22
AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   105   1e-21
CR954214_181(CR954214|pid:none) Ostreococcus tauri strain OTTH05...   105   1e-21
CP000881_15(CP000881|pid:none) Hemiselmis andersenii chromosome ...   103   2e-21
AF083031_87(AF083031|pid:none) Guillardia theta nucleomorph chro...   103   3e-21
A40411(A40411) translation elongation factor eEF-2 - Caenorhabdi...   102   5e-21
AF544973_1(AF544973|pid:none) Ctenopharyngodon idella elongation...   102   5e-21
protein update 2009. 7. 5
PSORT 

 psg: 0.84  gvh: 0.32  alm: 0.45  top: 0.30  tms: 0.00  mit: 0.24  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 28.0 %: nuclear
	 20.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VHF892 is cyt
5' end seq. ID VHF892F
5' end seq. 
>VHF892F.Seq
TCACCATTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAANGAAGACAAATTAC
CAGCTGGTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAAGTCACGTTG
ATTTCAGTTCNNNNNNNNNN
Length of 5' end seq. 140
3' end seq. ID VHF892Z
3' end seq. 
>VHF892Z.Seq
NNNNNNNNNNATGAATTAACTGCNTCCCCAAATATCTTAGAACCAATCTACTTAGTAGAA
ATTACTGCCCCAGAAAACGCTATTGGTGGTATTTANTCTGTANTCAATCGTCGTAGAGGT
ATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTATCTGTCAAAGCTCATTTA
CCAGTATTAGAATCNTTCGGTTTCACCGATGATTTACGTTCCCACACTGCCGGTCAAGNT
TTCCCACAATGTGTCTTCGATCANTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAA
GCCNCTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGAT
CTTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCATCTTTGAGTTTTCAA
Length of 3' end seq. 417
Connected seq. ID VHF892P
Connected seq. 
>VHF892P.Seq
TCACCATTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAANGAAGACAAATTAC
CAGCTGGTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAAGTCACGTTG
ATTTCAGTTC----------ATGAATTAACTGCNTCCCCAAATATCTTAGAACCAATCTA
CTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTANTCTGTANTCAATCG
TCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTATCTGTCAA
AGCTCATTTACCAGTATTAGAATCNTTCGGTTTCACCGATGATTTACGTTCCCACACTGC
CGGTCAAGNTTTCCCACAATGTGTCTTCGATCANTGGGCTTCAATTGGTGTTGTCAACAA
AGATAAGAAAGCCNCTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGA
AATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCATCTTTGAG
TTTTCAA
Length of connected seq. 537
Full length Seq ID -
Full length Seq. -
Length of full length seq. -