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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHF877
Library VH
(Link to library)
Clone ID VHF877
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16274-1
Original site URL
Representative seq. ID VHF877P
(Link to Original site)
Representative DNA sequence 
>VHF877 (VHF877Q) /CSM/VH/VHF8-D/VHF877Q.Seq.d/
AGTTATAATTTATAAATATAAAAAAAATGTCAAAAGCTAAAGATACAGGTATCTTAAGAT
TTGATGATACACCATTANCAGCAACATTTCCATTANTTGCTATTATCACATCAAAAGTTG
TTGGTGGTGTTAAAATCGTTGGTAGAAAAGGTTTAGATTCCACCGAATTCTCGATTGTCG
GTACTCAAGATTCACTCAAAGGTTCATATGTCATTGCAAAATATTTAGCAAGAACTACAC
CATCTCTTTCATTATATGGTGAAAATGCTTTATCAGCTTCAAAGATCGATGAATTTATTG
ATAAATTTGCTCATTTAAAATCAGAGAAATTCAATGAATTTTTAAAAGAAATGAATGAAT
ATTTAACATTAAGAGCATTTTTAATTGGATTTAATTTAACATTAGCAGATATTGTTTTAT
TTGCACGTATTAAGATGGTAAAGGAAATTCAAGANGAGATTAACAAATTANGTAAAACCA
TCCCACATTTAAATAGATGGTATGGATATTTAAGTCAATXXXXXXXXXXTTACCAGAGGT
CAAGGATTGTCTCAAATATGCAAAGGACCCATCAATGGGTACTAAAAAAGTTACATTCAG
TAATAATCTCTTCCTTGAAGGTGATGATTGTAATCAAATTAAAGAGGGTGAAGAAGTTAC
TNTCATGAATTGGGGTAATGCCATCGTTGAAACTTTACAACGTAACGAAAATGGTGATGT
TGTATCCATGACTGGTAAACTCCACTTGGAAGGTGATGTAAAGAAGACCGATAAAAAACT
CAGTTGGTTATCATCTGATTGTGCTGACACTGTCACAGTTGTCNTCCAAGATTACGATTA
TATCATCACTAAACCAAAATTAGAAGATGGTGATGATCTCGATACTTTCACCAATAAAAA
CTCCAAATTTGAAATTGAAGCTTTCACTGATGAAAATATCNTTANTCTCAAACTTAACGA
TAAAATTCAATTTGAGAGAAGAGGTTTCTTTAATGTTGATCAAGTTGGTGATGGTGTTAA
ACCATACATTTTAATCTATATTCCATCTGGTCCAATTAAACCAGCTGGTGCTGCTCTTTA
TCCATTCAAAAAAGTTGAAAAAGTTGCTGCTCCAGTCAATCCAAAACCAACTGCAAAAAA
ACAAGAAAAACAATCAAAGAAATAAAATAAATAATTTAAAT
sequence update 2002.10.25
Translated Amino Acid sequence 
l*FINIKKMSKAKDTGILRFDDTPLXATFPLXAIITSKVVGGVKIVGRKGLDSTEFSIVG
TQDSLKGSYVIAKYLARTTPSLSLYGENALSASKIDEFIDKFAHLKSEKFNEFLKEMNEY
LTLRAFLIGFNLTLADIVLFARIKMVKEIQXEINKLXKTIPHLNRWYGYLSQ---

---LPEVKDCLKYAKDPSMGTKKVTFSNNLFLEGDDCNQIKEGEEVTXMNWGNAIVETLQ
RNENGDVVSMTGKLHLEGDVKKTDKKLSWLSSDCADTVTVVXQDYDYIITKPKLEDGDDL
DTFTNKNSKFEIEAFTDENIXXLKLNDKIQFERRGFFNVDQVGDGVKPYILIYIPSGPIK
PAGAALYPFKKVEKVAAPVNPKPTAKKQEKQSKK*nk*fk
Translated Amino Acid sequence (All Frames) 
Frame A:
synl*i*kkcqklkiqvs*dlmihhxqqhfhxlllshqkllvvlkslvekv*ippnsrls
vlkihskvhmslqni*qelhhlfhymvkmlyqlqrsmnllinlli*nqrnsmnf*kk*mn
i*h*ehf*ldli*h*qilfylhvlrw*rkfkxrltnxvkpshi*idgmdi*vn---

---LPEVKDCLKYAKDPSMGTKKVTFSNNLFLEGDDCNQIKEGEEVTXMNWGNAIVETLQ
RNENGDVVSMTGKLHLEGDVKKTDKKLSWLSSDCADTVTVVXQDYDYIITKPKLEDGDDL
DTFTNKNSKFEIEAFTDENIXXLKLNDKIQFERRGFFNVDQVGDGVKPYILIYIPSGPIK
PAGAALYPFKKVEKVAAPVNPKPTAKKQEKQSKK*nk*fk

Frame B:
viiykykknvks*ryrylki**ytixsnisixcyyhikscwwc*nrw*krfrfhrildcr
ysrftqrfichckifsknytisfiiw*kcfisfkdr*iy**icsfkireiq*ifkrne*i
fniksifnwi*fnisrycficty*dgkgnsrxd*qix*nhptfk*mvwifks---

---yqrsrivsnmqrthqwvlkklhsviisslkvmiviklkrvkklls*igvmpslklyn
vtkmvmlyp*lvnstwkvm*rrpiknsvgyhlivltlsqlsskitiisslnqn*kmvmis
ilspiktpnlklklslmkislxsnltikfnlreevslmliklvmvlnhtf*sifhlvqln
qlvllfihskklkklllqsiqnqlqknkknnqrnkinnln

Frame C:
l*FINIKKMSKAKDTGILRFDDTPLXATFPLXAIITSKVVGGVKIVGRKGLDSTEFSIVG
TQDSLKGSYVIAKYLARTTPSLSLYGENALSASKIDEFIDKFAHLKSEKFNEFLKEMNEY
LTLRAFLIGFNLTLADIVLFARIKMVKEIQXEINKLXKTIPHLNRWYGYLSQ---

---trgqglsqickgpingy*ksyiq**slp*r**l*sn*rg*rsyxhelg*chr*nftt
*rkw*ccihdw*tplgr*ckedr*ktqlvii*lc*hchscxprlrlyhh*tkirrw**sr
yfhq*klqi*n*sfh**kyxxsqt*r*nsi*ekrfl*c*ssw*wc*tihfnlysiwsn*t
swccslsiqks*ksccssqsktnckktrktikeik*ii*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF877 (VHF877Q) /CSM/VH/VHF8-D/VHF877Q.Seq.d/                       2056   0.0
VHQ233 (VHQ233Q) /CSM/VH/VHQ2-B/VHQ233Q.Seq.d/                       1130   0.0
VHP868 (VHP868Q) /CSM/VH/VHP8-C/VHP868Q.Seq.d/                       1130   0.0
VHC684 (VHC684Q) /CSM/VH/VHC6-D/VHC684Q.Seq.d/                       1130   0.0
CHQ668 (CHQ668Q) /CSM/CH/CHQ6-C/CHQ668Q.Seq.d/                       1130   0.0
CHG848 (CHG848Q) /CSM/CH/CHG8-B/CHG848Q.Seq.d/                       1130   0.0
CFK330 (CFK330Q) /CSM/CF/CFK3-B/CFK330Q.Seq.d/                       1130   0.0
AHO133 (AHO133Q) /CSM/AH/AHO1-B/AHO133Q.Seq.d/                       1130   0.0
AHM642 (AHM642Q) /CSM/AH/AHM6-B/AHM642Q.Seq.d/                       1130   0.0
AHL424 (AHL424Q) /CSM/AH/AHL4-A/AHL424Q.Seq.d/                       1130   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ440174) Dictyostelium discoideum cDNA clone:ddv42j20, 3' ...  1080   0.0    1
(BJ446815) Dictyostelium discoideum cDNA clone:ddv63m24, 3' ...  1072   0.0    1
(BJ446755) Dictyostelium discoideum cDNA clone:ddv63b19, 3' ...  1072   0.0    1
(BJ446599) Dictyostelium discoideum cDNA clone:ddv63a10, 3' ...  1072   0.0    1
(BJ446400) Dictyostelium discoideum cDNA clone:ddv62h18, 3' ...  1072   0.0    1
(BJ445846) Dictyostelium discoideum cDNA clone:ddv60g21, 3' ...  1072   0.0    1
(BJ445205) Dictyostelium discoideum cDNA clone:ddv58d20, 3' ...  1072   0.0    1
(BJ443159) Dictyostelium discoideum cDNA clone:ddv52g05, 3' ...  1072   0.0    1
(BJ442811) Dictyostelium discoideum cDNA clone:ddv51d06, 3' ...  1072   0.0    1
(BJ441496) Dictyostelium discoideum cDNA clone:ddv46k23, 3' ...  1072   0.0    1
dna update 2008.10.31
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54KB8) RecName: Full=Probable glutamyl-tRNA synthetase, cytopl...   395   e-108
FN392319_1395(FN392319|pid:none) Pichia pastoris GS115 chromosom...   152   2e-35
AP007162_467(AP007162|pid:none) Aspergillus oryzae RIB40 genomic...   147   9e-34
AM920436_1576(AM920436|pid:none) Penicillium chrysogenum Wiscons...   145   3e-33
CR382131_1161(CR382131|pid:none) Yarrowia lipolytica strain CLIB...   144   8e-33
CR382121_27(CR382121|pid:none) Kluyveromyces lactis strain NRRL ...   142   2e-32
CP000499_571(CP000499|pid:none) Pichia stipitis CBS 6054 chromos...   140   9e-32
FM992694_4(FM992694|pid:none) Candida dubliniensis CD36 chromoso...   140   9e-32
(O13775) RecName: Full=Probable glutamyl-tRNA synthetase, cytopl...   139   1e-31
AP006852_60(AP006852|pid:none) Candida albicans genomic DNA, chr...   137   7e-31
protein update 2009. 7. 5
PSORT 

 psg: 0.56  gvh: 0.50  alm: 0.29  top: 0.67  tms: 0.07  mit: 0.23  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.80  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: nuclear
	 24.0 %: mitochondrial
	 24.0 %: cytoplasmic
	  8.0 %: plasma membrane
	  8.0 %: endoplasmic reticulum
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for VHF877 is nuc
5' end seq. ID VHF877F
5' end seq. 
>VHF877F.Seq
AGTTATAATTTATAAATATAAAAAAAATGTCAAAAGCTAAAGATACAGGTATCTTAAGAT
TTGATGATACACCATTANCAGCAACATTTCCATTANTTGCTATTATCACATCAAAAGTTG
TTGGTGGTGTTAAAATCGTTGGTAGAAAAGGTTTAGATTCCACCGAATTCTCGATTGTCG
GTACTCAAGATTCACTCAAAGGTTCATATGTCATTGCAAAATATTTAGCAAGAACTACAC
CATCTCTTTCATTATATGGTGAAAATGCTTTATCAGCTTCAAAGATCGATGAATTTATTG
ATAAATTTGCTCATTTAAAATCAGAGAAATTCAATGAATTTTTAAAAGAAATGAATGAAT
ATTTAACATTAAGAGCATTTTTAATTGGATTTAATTTAACATTAGCAGATATTGTTTTAT
TTGCACGTATTAAGATGGTAAAGGAAATTCAAGANGAGATTAACAAATTANGTAAAACCA
TCCCACATTTAAATAGATGGTATGGATATTTAAGTCAATNNNNNNNNNN
Length of 5' end seq. 529
3' end seq. ID VHF877Z
3' end seq. 
>VHF877Z.Seq
NNNNNNNNNNTTACCAGAGGTCAAGGATTGTCTCAAATATGCAAAGGACCCATCAATGGG
TACTAAAAAAGTTACATTCAGTAATAATCTCTTCCTTGAAGGTGATGATTGTAATCAAAT
TAAAGAGGGTGAAGAAGTTACTNTCATGAATTGGGGTAATGCCATCGTTGAAACTTTACA
ACGTAACGAAAATGGTGATGTTGTATCCATGACTGGTAAACTCCACTTGGAAGGTGATGT
AAAGAAGACCGATAAAAAACTCAGTTGGTTATCATCTGATTGTGCTGACACTGTCACAGT
TGTCNTCCAAGATTACGATTATATCATCACTAAACCAAAATTAGAAGATGGTGATGATCT
CGATACTTTCACCAATAAAAACTCCAAATTTGAAATTGAAGCTTTCACTGATGAAAATAT
CNTTANTCTCAAACTTAACGATAAAATTCAATTTGAGAGAAGAGGTTTCTTTAATGTTGA
TCAAGTTGGTGATGGTGTTAAACCATACATTTTAATCTATATTCCATCTGGTCCAATTAA
ACCAGCTGGTGCTGCTCTTTATCCATTCAAAAAAGTTGAAAAAGTTGCTGCTCCAGTCAA
TCCAAAACCAACTGCAAAAAAACAAGAAAAACAATCAAAGAAATAAAATAAATAATTTAA
AT
Length of 3' end seq. 662
Connected seq. ID VHF877P
Connected seq. 
>VHF877P.Seq
AGTTATAATTTATAAATATAAAAAAAATGTCAAAAGCTAAAGATACAGGTATCTTAAGAT
TTGATGATACACCATTANCAGCAACATTTCCATTANTTGCTATTATCACATCAAAAGTTG
TTGGTGGTGTTAAAATCGTTGGTAGAAAAGGTTTAGATTCCACCGAATTCTCGATTGTCG
GTACTCAAGATTCACTCAAAGGTTCATATGTCATTGCAAAATATTTAGCAAGAACTACAC
CATCTCTTTCATTATATGGTGAAAATGCTTTATCAGCTTCAAAGATCGATGAATTTATTG
ATAAATTTGCTCATTTAAAATCAGAGAAATTCAATGAATTTTTAAAAGAAATGAATGAAT
ATTTAACATTAAGAGCATTTTTAATTGGATTTAATTTAACATTAGCAGATATTGTTTTAT
TTGCACGTATTAAGATGGTAAAGGAAATTCAAGANGAGATTAACAAATTANGTAAAACCA
TCCCACATTTAAATAGATGGTATGGATATTTAAGTCAAT----------TTACCAGAGGT
CAAGGATTGTCTCAAATATGCAAAGGACCCATCAATGGGTACTAAAAAAGTTACATTCAG
TAATAATCTCTTCCTTGAAGGTGATGATTGTAATCAAATTAAAGAGGGTGAAGAAGTTAC
TNTCATGAATTGGGGTAATGCCATCGTTGAAACTTTACAACGTAACGAAAATGGTGATGT
TGTATCCATGACTGGTAAACTCCACTTGGAAGGTGATGTAAAGAAGACCGATAAAAAACT
CAGTTGGTTATCATCTGATTGTGCTGACACTGTCACAGTTGTCNTCCAAGATTACGATTA
TATCATCACTAAACCAAAATTAGAAGATGGTGATGATCTCGATACTTTCACCAATAAAAA
CTCCAAATTTGAAATTGAAGCTTTCACTGATGAAAATATCNTTANTCTCAAACTTAACGA
TAAAATTCAATTTGAGAGAAGAGGTTTCTTTAATGTTGATCAAGTTGGTGATGGTGTTAA
ACCATACATTTTAATCTATATTCCATCTGGTCCAATTAAACCAGCTGGTGCTGCTCTTTA
TCCATTCAAAAAAGTTGAAAAAGTTGCTGCTCCAGTCAATCCAAAACCAACTGCAAAAAA
ACAAGAAAAACAATCAAAGAAATAAAATAAATAATTTAAAT
Length of connected seq. 1171
Full length Seq ID -
Full length Seq. -
Length of full length seq. -