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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHF832
Library VH
(Link to library)
Clone ID VHF832
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VHF832P
(Link to Original site)
Representative DNA sequence 
>VHF832 (VHF832Q) /CSM/VH/VHF8-B/VHF832Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGANCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTGATCAATCTTATCGATTCCCCANGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTANTCGTTATCGATTXXX
XXXXXXXATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAGGTAACG
GTGCCAATCTCTTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGATT
CTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGA
GAGGTAANCGTTTCAATCTTTATGATGTCACCNTCCACACTGATGCCATCCACAGAGGTG
GTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCCC
CAACTATCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTG
GTATTTANTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCG
GTTCCCCATTATTATCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCG
ATGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGG
CTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAA
AGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAA
CAATTTAAGTCGCACTTTGAGTTTTCAA
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQXRGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPXHVDFS
SEVTAALRVTDGALXVID---

---WDKNDAMNIWSFGPEGNGANLFVNVTKGVQYLNEIKDSFVGAFQWATKEGVVCDENM
RGXRFNLYDVTXHTDAIHRGGGQIIPTARRVLYAAELTASPTILEPIYLVEITAPENAIG
GIXSVLNRRRGIVIGEERRIGSPLLSVKAHLPVLESFGFTDDLRSHTAGQAFPQCVFDHW
ASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*ti*val*vf
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnkxvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipxvtlis
vqk*llpsvllmvlxslsi---

---mg*k*chehlvfrsrr*rcqslrqchqrcsife*n*rffrwcfpmghqrrccl**kh
er*xfqsl*chxph*chpqrwwsnhpncssctlcc*inclpnylrtnllsrnycprkryw
wylxctqss*rycyw*rtsyrfpiiicqssftsirilrfhr*ftfphcrssfptmclrsl
gfnwccqqr*esh*srfshqkekrfsprnprs*qiprktlnnlsrtlsfq

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trxwyhh*ificitslrnakgrqitswlyftrifdqsyrfpxsr*fq
frsnccppcy*wcfxryr---

---WDKNDAMNIWSFGPEGNGANLFVNVTKGVQYLNEIKDSFVGAFQWATKEGVVCDENM
RGXRFNLYDVTXHTDAIHRGGGQIIPTARRVLYAAELTASPTILEPIYLVEITAPENAIG
GIXSVLNRRRGIVIGEERRIGSPLLSVKAHLPVLESFGFTDDLRSHTAGQAFPQCVFDHW
ASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*ti*val*vf

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQXRGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPXHVDFS
SEVTAALRVTDGALXVID---

---gikmmp*tfglsvqkvtvpisssmspkvfni*mklkilslvlsngppkkvlfvmkt*
evxvsifmmspstlmpstevvvkssqllvvysmlln*lppqls*nqst**kllpqktllv
vfxlysivvevlllvknvvsvphyylskliyqy*npsvspmiyvptlpvklshnvssiig
lqlvlstkirkplksl*pperekv*pqksqiltnstknskqfkshfefs
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF832 (VHF832Q) /CSM/VH/VHF8-B/VHF832Q.Seq.d/                       2062   0.0
VHE779 (VHE779Q) /CSM/VH/VHE7-D/VHE779Q.Seq.d/                       1308   0.0
VHE707 (VHE707Q) /CSM/VH/VHE7-A/VHE707Q.Seq.d/                       1304   0.0
VHP487 (VHP487Q) /CSM/VH/VHP4-D/VHP487Q.Seq.d/                       1292   0.0
VHP408 (VHP408Q) /CSM/VH/VHP4-A/VHP408Q.Seq.d/                       1292   0.0
VHO803 (VHO803Q) /CSM/VH/VHO8-A/VHO803Q.Seq.d/                       1292   0.0
VHO104 (VHO104Q) /CSM/VH/VHO1-A/VHO104Q.Seq.d/                       1292   0.0
VHN730 (VHN730Q) /CSM/VH/VHN7-B/VHN730Q.Seq.d/                       1292   0.0
VHJ871 (VHJ871Q) /CSM/VH/VHJ8-C/VHJ871Q.Seq.d/                       1292   0.0
VHI504 (VHI504Q) /CSM/VH/VHI5-A/VHI504Q.Seq.d/                       1292   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ440044) Dictyostelium discoideum cDNA clone:ddv42p08, 3' ...  1332   0.0    1
(BJ439350) Dictyostelium discoideum cDNA clone:ddv40n01, 3' ...  1304   0.0    1
(BJ439582) Dictyostelium discoideum cDNA clone:ddv40n19, 3' ...  1302   0.0    1
(BJ437610) Dictyostelium discoideum cDNA clone:ddv34e21, 3' ...  1294   0.0    1
(AU039435) Dictyostelium discoideum slug cDNA, clone SLH782.     1292   0.0    1
(BJ446187) Dictyostelium discoideum cDNA clone:ddv61n22, 3' ...  1292   0.0    1
(BJ445965) Dictyostelium discoideum cDNA clone:ddv61p02, 3' ...  1292   0.0    1
(BJ445613) Dictyostelium discoideum cDNA clone:ddv60f02, 3' ...  1292   0.0    1
(BJ445302) Dictyostelium discoideum cDNA clone:ddv59g01, 3' ...  1292   0.0    1
(BJ445085) Dictyostelium discoideum cDNA clone:ddv58l07, 3' ...  1292   0.0    1
dna update 2008.10.31
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   270   7e-71
AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   269   1e-70
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   268   2e-70
BT071866_1(BT071866|pid:none) Salmo salar clone ssal-rgf-501-088...   267   4e-70
BT059038_1(BT059038|pid:none) Salmo salar clone ssal-rgf-521-328...   267   4e-70
BT045031_1(BT045031|pid:none) Salmo salar clone ssal-rgf-510-120...   266   7e-70
BT072304_1(BT072304|pid:none) Salmo salar clone ssal-rgf-520-280...   266   1e-69
BC060707_1(BC060707|pid:none) Mus musculus eukaryotic translatio...   265   3e-69
AK161993_1(AK161993|pid:none) Mus musculus 12 days embryo male w...   265   3e-69
AK298498_1(AK298498|pid:none) Homo sapiens cDNA FLJ56548 complet...   265   3e-69
protein update 2009. 7. 5
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 32.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: extracellular, including cell wall

>> prediction for VHF832 is nuc
5' end seq. ID VHF832F
5' end seq. 
>VHF832F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGANCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTGATCAATCTTATCGATTCCCCANGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTANTCGTTATCGATTNNN
NNNNNNN
Length of 5' end seq. 427
3' end seq. ID VHF832Z
3' end seq. 
>VHF832Z.Seq
NNNNNNNNNNATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAGGTA
ACGGTGCCAATCTCTTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAG
ATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACA
TGAGAGGTAANCGTTTCAATCTTTATGATGTCACCNTCCACACTGATGCCATCCACAGAG
GTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCT
CCCCAACTATCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTG
GTGGTATTTANTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTA
TCGGTTCCCCATTATTATCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCA
CCGATGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATT
GGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCA
GAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCT
AAACAATTTAAGTCGCACTTTGAGTTTTCAA
Length of 3' end seq. 691
Connected seq. ID VHF832P
Connected seq. 
>VHF832P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGANCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTGATCAATCTTATCGATTCCCCANGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTANTCGTTATCGATT---
-------ATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAGGTAACG
GTGCCAATCTCTTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGATT
CTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGA
GAGGTAANCGTTTCAATCTTTATGATGTCACCNTCCACACTGATGCCATCCACAGAGGTG
GTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCCC
CAACTATCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTG
GTATTTANTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCG
GTTCCCCATTATTATCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCG
ATGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGG
CTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAA
AGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAA
CAATTTAAGTCGCACTTTGAGTTTTCAA
Length of connected seq. 1098
Full length Seq ID -
Full length Seq. -
Length of full length seq. -