>AFN831 (AFN831Q) /CSM/AF/AFN8-B/AFN831Q.Seq.d/
XXXXXXXXXXTATTTTACAATTAGCATCTGAAGGTGCACTCTCACTCAAAGAGATTAAAC
AATTCATTCTTGATGAATGTGATACCATGCTCGAATCATTGGATATGAGAAAGGATGTAC
AAAAGATCTTCAAACTCATCCCAGCCAACAAACAAGTTATGATGTTTTCAGCTACCCTCT
CTGATACCATTCGTCCAATCTGTAAGAAGTTTATGAACAATCCATTGGAAATCTACATCA
ATGATGGTAGTAAACTCACTCTCCATGGTCTTCAACAATACTACGTACCAATCACTGAAG
AACAAAAGAATAAAAAACTCATTGAACTCTTGGATTCATTAGACTTTAATCAAGCCGTTA
TTTTCGTCAAATCTGTTAGACGTGCTGATGCCTTAAATAAGATCCTCCAAGATATTGGTT
TCCCATCCATCTGTATTCATCGTGACCTCGATCAAAAGGATCGTATCGAACAATACCGTA
AATTCAAAAACTTTGAATCTCGTATCATGGTTGCCACTAATATCTTTGGTCGTGGTATCG
ATATTGAACGTGTCAATGTCGTCATCAACTACGATATGGCTGAA

---ILQLASEGALSLKEIKQFILDECDTMLESLDMRKDVQKIFKLIPANKQVMMFSATLS
DTIRPICKKFMNNPLEIYINDGSKLTLHGLQQYYVPITEEQKNKKLIELLDSLDFNQAVI
FVKSVRRADALNKILQDIGFPSICIHRDLDQKDRIEQYRKFKNFESRIMVATNIFGRGID
IERVNVVINYDMAE

Frame A:
---ILQLASEGALSLKEIKQFILDECDTMLESLDMRKDVQKIFKLIPANKQVMMFSATLS
DTIRPICKKFMNNPLEIYINDGSKLTLHGLQQYYVPITEEQKNKKLIELLDSLDFNQAVI
FVKSVRRADALNKILQDIGFPSICIHRDLDQKDRIEQYRKFKNFESRIMVATNIFGRGID
IERVNVVINYDMAE


Frame B:
---fyn*hlkvhshskrlnnsflmnvipcsnhwi*ermykrssnssqptnkl*cfqlpsl
ipfvqsvrsl*tihwkstsmmvvnslsmvfnnttyqslknkriknslnswih*tlikplf
ssnlldvlmp*irsskilvshpsvfivtsikrivsnntvnsktlnlvswlplislvvvsi
lnvsmsssttiwl


Frame C:
---ftisi*rctltqrd*tihs**m*yhariigyekgctkdlqthpsqqtsydvfsypl*
yhssnl*evyeqsignlhq*w**thspwsstilrtnh*rtke*kth*tlgfirl*ssryf
rqic*tc*clk*dpprywfpihlyss*prskgsyrtip*iqkl*isyhgch*ylwswyry
*tcqcrhqlryg*

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFE359 (VFE359Q) /CSM/VF/VFE3-C/VFE359Q.Seq.d/                       1138   0.0
VFD348 (VFD348Q) /CSM/VF/VFD3-B/VFD348Q.Seq.d/                       1138   0.0
VFD181 (VFD181Q) /CSM/VF/VFD1-D/VFD181Q.Seq.d/                       1138   0.0
SFG247 (SFG247Q) /CSM/SF/SFG2-B/SFG247Q.Seq.d/                       1138   0.0
SFF569 (SFF569Q) /CSM/SF/SFF5-C/SFF569Q.Seq.d/                       1138   0.0
FC-AI06 (FC-AI06Q) /CSM/FC/FC-AI/FC-AI06Q.Seq.d/                     1138   0.0
AFO440 (AFO440Q) /CSM/AF/AFO4-B/AFO440Q.Seq.d/                       1138   0.0
AFN831 (AFN831Q) /CSM/AF/AFN8-B/AFN831Q.Seq.d/                       1138   0.0
AFM640 (AFM640Q) /CSM/AF/AFM6-B/AFM640Q.Seq.d/                       1138   0.0
AFM164 (AFM164Q) /CSM/AF/AFM1-C/AFM164Q.Seq.d/                       1138   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AL408654|AL408654.1 T7 end of clone AV0AA009H09 of library AV0AA from strain CBS 379 of Saccharomyces exiguus.    38  7e-10  4
AX488925|AX488925.1 Sequence 6225 from Patent WO02053728.         40  8e-10  5
BF071471|BF071471.1 st59b09.y1 Gm-c1053 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1053-857 5' similar to TR:Q9ZS06 Q9ZS06 RNA HELICASE ;, mRNA sequence.    56  1e-08  2
AW759959|AW759959.1 sl56c07.y1 Gm-c1027 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1027-5317 5' similar to TR:Q9ZS06 Q9ZS06 RNA HELICASE ;, mRNA sequence.    56  1e-08  2
BI742220|BI742220.1 kt91h02.y1 Strongyloides ratti L3 adult pAMP1 v1 Chiapelli McCarter Strongyloides ratti cDNA 5' similar to SW:HE47_CAEEL Q18212 PROBABLE ATP-DEPENDENT RNA HELICASE P47 HOMOLOG. [1] ;, mRNA sequence.    38  5e-08  4
BI974435|BI974435.1 saj01e10.y1 Gm-c1065 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1065-9068 5' similar to TR:Q9ZS06 Q9ZS06 RNA HELICASE ;, mRNA sequence.    52  2e-07  2
Z74132|Z74132.1 S.cerevisiae chromosome IV reading frame ORF YDL084w.    42  2e-07  4
AI966749|AI966749.1 sc57c05.y1 Gm-c1016 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1016-513 5' similar to TR:O13792 O13792 PUTATIVE ATP-DEPENDENT RNA HELICASE C17G6.14C. ;, mRNA sequence.    48  1e-06  2
AK067431|AK067431.1 Oryza sativa (japonica cultivar-group) cDNA clone:J013107H03, full insert sequence.    40  2e-06  3
BF936994|BF936994.1 EST460049 Schistosoma mansoni female, Phil LoVerde/Joe Merrick Schistosoma mansoni cDNA clone SMFCE26 5' end, mRNA sequence.    64  2e-06  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AJ010466_1(AJ010466|pid:none) Arabidopsis thaliana mRNA for DEAD...   256   4e-67
GM694469_1(GM694469|pid:none) Sequence 25 from Patent EP1953235.      254   1e-66
(O13792) RecName: Full=ATP-dependent RNA helicase uap56;        ...   254   1e-66
D89270_1(D89270|pid:none) Schizosaccharomyces pombe mRNA, partia...   253   2e-66
GM694505_1(GM694505|pid:none) Sequence 61 from Patent EP1953235....   253   2e-66
(A1DL85) RecName: Full=ATP-dependent RNA helicase sub2;         ...   253   2e-66
(Q1DI07) RecName: Full=ATP-dependent RNA helicase SUB2;         ...   253   3e-66
BT068295_1(BT068295|pid:none) Zea mays full-length cDNA clone ZM...   253   3e-66
AJ428513_1(AJ428513|pid:none) Chironomus tentans mRNA for HEL pr...   253   3e-66
(Q56XG6) RecName: Full=DEAD-box ATP-dependent RNA helicase 15;  ...   252   4e-66

 psg: 0.75  gvh: 0.37  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 32.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for AFN831 is cyt

>AFN831Z.Seq
----------TATTTTACAATTAGCATCTGAAGGTGCACTCTCACTCAAAGAGATTAAAC
AATTCATTCTTGATGAATGTGATACCATGCTCGAATCATTGGATATGAGAAAGGATGTAC
AAAAGATCTTCAAACTCATCCCAGCCAACAAACAAGTTATGATGTTTTCAGCTACCCTCT
CTGATACCATTCGTCCAATCTGTAAGAAGTTTATGAACAATCCATTGGAAATCTACATCA
ATGATGGTAGTAAACTCACTCTCCATGGTCTTCAACAATACTACGTACCAATCACTGAAG
AACAAAAGAATAAAAAACTCATTGAACTCTTGGATTCATTAGACTTTAATCAAGCCGTTA
TTTTCGTCAAATCTGTTAGACGTGCTGATGCCTTAAATAAGATCCTCCAAGATATTGGTT
TCCCATCCATCTGTATTCATCGTGACCTCGATCAAAAGGATCGTATCGAACAATACCGTA
AATTCAAAAACTTTGAATCTCGTATCATGGTTGCCACTAATATCTTTGGTCGTGGTATCG
ATATTGAACGTGTCAATGTCGTCATCAACTACGATATGGCTGAA