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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHF730
Library VH
(Link to library)
Clone ID VHF730
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15767-1
Original site URL
Representative seq. ID VHF730P
(Link to Original site)
Representative DNA sequence 
>VHF730 (VHF730Q) /CSM/VH/VHF7-B/VHF730Q.Seq.d/
TTTACCACTCATCAAAAAACAACAAATAAAAAATAAGAAAGTCTAAAATATTAAAAGAAT
AAAAAAAATATATACAATAAAATAAAAAAACAAAATGTTCATTCAAAGAACATTTTTAAA
AGTTCTTACTCTTTTGAGTATAGTAACAGTTTTGGTCCATGGACAAACTCAACCAAAAGA
TAAAATTACATTGAAGGCCGTAATTTATGATCAACACAAGTTTTATAATCCAAATTTCCA
ACCAAAAAATGAGGGAGAATTTGTTTTAACTAAGGGAATTGTCAAATCAGATATTGATCA
AGAAAAAAGAATTCCAGTTTTAAATAGTATGGATGCCAATGATTCTATAAATAAAAAGGC
AAGAATTAGTTGGCCAGATGGTTTTAAGTATTTTTTTGTAGATAACCAAGCTGGTGATTC
AGAATCTGCCAAATCTGGTAAAAATCTTCCAATTCAAAGAGATATCGAGTTAAATTGGAA
TGGAGAAGCATATGAATATAGTAATTCAAATTACTTCCCAATTAACGGACAANGTTTCAA
TGATGTAAGCTATCCAGTTXXXXXXXXXXGTTACATGTGGTGGTTGTGAAACNTGTTCAT
ACGCTACCGGTAAATGTGAACCAGATAGTTCACTTTGTAATGATAACAATATTTGTACCA
TTGATATTTGTGTCCATGAAGGTATTCTCGATGGTCTACCACAAGGTAATTGTTCAAATA
CTCCAGTTGATTGTGGTGCCAATGATGAAGATAAATGTAAAACTTGGTCTTGTGATCCAA
NTAAAGGTGGCTGTCAATCAACCCCAGTCGTTTGTGAAGATAAAGGTAAATGTTTAGTTG
GTACTTGTCAACCATCCACTGGTCAATGTGAATACTCTGATAGAGTTTGTGATAATGGTG
GTGCTTTCTGTGTTATTGGCCAATGTGATCAAAGACTTGGTTGTTTAGTTTTCGATAGAG
TTTGTTCATCTGATAATAGTAGATGTGAAGAAGGTGTTTGTGTAAATGGTACTGAATCTG
AAGAAGGTCATTGTAAATCTGTTAAATATGACCCATTACCATTCAATTGTAATACTGGTG
CTGTTGTTTCAACTGCTGTTATTGCTGGTTCTACTGTCGCTGGTGCTGTTGCTCTTGGTA
TTTTCCTTTATGGTGGTAAAAAAGGTTATGACTATTGGAAAGACTCAAGAAATATTTCAA
TGGGCTCATCAAATAGTAATCCATTGTATGAAGAACAACAAACGGAAGAGGTGTCAACCC
AATGTAGNACGATCCAG
sequence update 2002.10.25
Translated Amino Acid sequence 
lplikkqqiknkkv*nikrikkiytik*KNKMFIQRTFLKVLTLLSIVTVLVHGQTQPKD
KITLKAVIYDQHKFYNPNFQPKNEGEFVLTKGIVKSDIDQEKRIPVLNSMDANDSINKKA
RISWPDGFKYFFVDNQAGDSESAKSGKNLPIQRDIELNWNGEAYEYSNSNYFPINGQXFN
DVSYPV---

---VTCGGCETCSYATGKCEPDSSLCNDNNICTIDICVHEGILDGLPQGNCSNTPVDCGA
NDEDKCKTWSCDPXKGGCQSTPVVCEDKGKCLVGTCQPSTGQCEYSDRVCDNGGAFCVIG
QCDQRLGCLVFDRVCSSDNSRCEEGVCVNGTESEEGHCKSVKYDPLPFNCNTGAVVSTAV
IAGSTVAGAVALGIFLYGGKKGYDYWKDSRNISMGSSNSNPLYEEQQTEEVSTQCXTIQ
Translated Amino Acid sequence (All Frames) 
Frame A:
ftthqkttnkk*eslky*knkkniynkikkqnvhsknifkssysfeysnsfgpwtnstkr
*nyiegrnl*stqvl*skfptkk*gricfn*gncqiry*srkknssfk*ygcq*fyk*kg
kn*larwf*vffcr*psw*fricqiw*kssnskryrvklewrsi*i**fkllpn*rtxfq
*cklss---

---VTCGGCETCSYATGKCEPDSSLCNDNNICTIDICVHEGILDGLPQGNCSNTPVDCGA
NDEDKCKTWSCDPXKGGCQSTPVVCEDKGKCLVGTCQPSTGQCEYSDRVCDNGGAFCVIG
QCDQRLGCLVFDRVCSSDNSRCEEGVCVNGTESEEGHCKSVKYDPLPFNCNTGAVVSTAV
IAGSTVAGAVALGIFLYGGKKGYDYWKDSRNISMGSSNSNPLYEEQQTEEVSTQCXTIQ

Frame B:
lplikkqqiknkkv*nikrikkiytik*KNKMFIQRTFLKVLTLLSIVTVLVHGQTQPKD
KITLKAVIYDQHKFYNPNFQPKNEGEFVLTKGIVKSDIDQEKRIPVLNSMDANDSINKKA
RISWPDGFKYFFVDNQAGDSESAKSGKNLPIQRDIELNWNGEAYEYSNSNYFPINGQXFN
DVSYPV---

---lhvvvvkxvhtlpvnvnqivhfvmitifvplifvsmkvfsmvyhkvivqilqlivvp
mmkinvklglviqxkvavnqpqsfvkikvnv*lvlvnhplvnvntliefvimvvlsvlla
nvikdlvv*fsiefvhliivdvkkvfv*mvlnlkkvivnllnmthyhsivilvllfqlll
llvllslvllllvfsfmvvkkvmtigktqeifqwahqivihcmknnkrkrcqpnvxrs

Frame C:
yhssknnk*kirkskilke*kkyiq*nkktkcsfkehf*kfllf*v**qfwsmdklnqki
klh*rp*fmintsfiiqisnqkmrenlf*lrelsnqilikkkefqf*ivwmpmil*ikrq
elvgqmvlsifl*itklviqnlpnlvkifqfkeiss*igmekhmniviqitsqltdxvsm
m*aiq---

---ymwwl*nxfiryr*m*tr*ftl***qylyh*ylcp*rysrwsttr*lfkyss*lwcq
**r*m*nlvl*sx*rwlsinpsrl*r*r*mfswylstihwsm*il**sl**wwcflcywp
m*sktwlfsfr*slfi****m*rrclckwy*i*rrsl*ic*i*pitiql*ywcccfnccy
cwfycrwcccswyfplww*krl*llerlkkyfnglik**siv*rttngrgvnpm*xdp
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF730 (VHF730Q) /CSM/VH/VHF7-B/VHF730Q.Seq.d/                       2218   0.0
AHL481 (AHL481Q) /CSM/AH/AHL4-D/AHL481Q.Seq.d/                       1378   0.0
VHI218 (VHI218Q) /CSM/VH/VHI2-A/VHI218Q.Seq.d/                       1372   0.0
VHQ393 (VHQ393Q) /CSM/VH/VHQ3-D/VHQ393Q.Seq.d/                       1370   0.0
VHP613 (VHP613Q) /CSM/VH/VHP6-A/VHP613Q.Seq.d/                       1370   0.0
VHA622 (VHA622Q) /CSM/VH/VHA6-A/VHA622Q.Seq.d/                       1370   0.0
VHN636 (VHN636Q) /CSM/VH/VHN6-B/VHN636Q.Seq.d/                       1368   0.0
VHC809 (VHC809Q) /CSM/VH/VHC8-A/VHC809Q.Seq.d/                       1362   0.0
VHJ737 (VHJ737Q) /CSM/VH/VHJ7-B/VHJ737Q.Seq.d/                       1356   0.0
VHC841 (VHC841Q) /CSM/VH/VHC8-B/VHC841Q.Seq.d/                       1354   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ440023) Dictyostelium discoideum cDNA clone:ddv42l07, 3' ...  1384   0.0    1
(BJ354479) Dictyostelium discoideum cDNA clone:dda53b22, 3' ...  1376   0.0    1
(BJ441540) Dictyostelium discoideum cDNA clone:ddv47c06, 3' ...  1370   0.0    1
(BJ446801) Dictyostelium discoideum cDNA clone:ddv63j23, 3' ...  1368   0.0    1
(BJ446243) Dictyostelium discoideum cDNA clone:ddv62i04, 3' ...  1368   0.0    1
(BJ436757) Dictyostelium discoideum cDNA clone:ddv32k06, 3' ...  1368   0.0    1
(BJ445062) Dictyostelium discoideum cDNA clone:ddv58g10, 3' ...  1362   0.0    1
(BJ437985) Dictyostelium discoideum cDNA clone:ddv36b04, 3' ...  1360   0.0    1
(BJ438065) Dictyostelium discoideum cDNA clone:ddv36b12, 3' ...  1352   0.0    1
(BJ442594) Dictyostelium discoideum cDNA clone:ddv50j09, 3' ...  1328   0.0    2
dna update 2008.10.31
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54VS2) RecName: Full=Protein psiP; Flags: Precursor;                492   e-137
(Q94494) RecName: Full=Protein psiH; Flags: Precursor;  &U67940_...   254   6e-66
(Q86HT1) RecName: Full=Protein psiL; Flags: Precursor;  &AC11695...   246   1e-63
(Q54P69) RecName: Full=Protein psiM; Flags: Precursor;                204   7e-51
(Q557E9) RecName: Full=Protein psiG; Flags: Precursor;  &AC11568...   200   8e-50
(Q54G85) RecName: Full=Protein psiJ; Flags: Precursor;                146   1e-33
(Q54CH8) RecName: Full=Protein psiN; Flags: Precursor;                132   2e-29
S44208(S44208) extracellular matrix protein B - Dictyostelium mi...    86   2e-15
AC116984_103(AC116984|pid:none) Dictyostelium discoideum chromos...    83   2e-14
(P11976) RecName: Full=Prestalk protein; AltName: Full=Extracell...    82   3e-14
protein update 2009. 7. 5
PSORT 

 psg: 0.84  gvh: 0.70  alm: 0.27  top: 0.37  tms: 0.13  mit: 0.40  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 32.0 %: mitochondrial
	 32.0 %: endoplasmic reticulum
	 12.0 %: plasma membrane
	  8.0 %: extracellular, including cell wall
	  4.0 %: vacuolar
	  4.0 %: Golgi
	  4.0 %: nuclear
	  4.0 %: vesicles of secretory system

>> prediction for VHF730 is mit
5' end seq. ID VHF730F
5' end seq. 
>VHF730F.Seq
TTTACCACTCATCAAAAAACAACAAATAAAAAATAAGAAAGTCTAAAATATTAAAAGAAT
AAAAAAAATATATACAATAAAATAAAAAAACAAAATGTTCATTCAAAGAACATTTTTAAA
AGTTCTTACTCTTTTGAGTATAGTAACAGTTTTGGTCCATGGACAAACTCAACCAAAAGA
TAAAATTACATTGAAGGCCGTAATTTATGATCAACACAAGTTTTATAATCCAAATTTCCA
ACCAAAAAATGAGGGAGAATTTGTTTTAACTAAGGGAATTGTCAAATCAGATATTGATCA
AGAAAAAAGAATTCCAGTTTTAAATAGTATGGATGCCAATGATTCTATAAATAAAAAGGC
AAGAATTAGTTGGCCAGATGGTTTTAAGTATTTTTTTGTAGATAACCAAGCTGGTGATTC
AGAATCTGCCAAATCTGGTAAAAATCTTCCAATTCAAAGAGATATCGAGTTAAATTGGAA
TGGAGAAGCATATGAATATAGTAATTCAAATTACTTCCCAATTAACGGACAANGTTTCAA
TGATGTAAGCTATCCAGTTNNNNNNNNNN
Length of 5' end seq. 569
3' end seq. ID VHF730Z
3' end seq. 
>VHF730Z.Seq
NNNNNNNNNNGTTACATGTGGTGGTTGTGAAACNTGTTCATACGCTACCGGTAAATGTGA
ACCAGATAGTTCACTTTGTAATGATAACAATATTTGTACCATTGATATTTGTGTCCATGA
AGGTATTCTCGATGGTCTACCACAAGGTAATTGTTCAAATACTCCAGTTGATTGTGGTGC
CAATGATGAAGATAAATGTAAAACTTGGTCTTGTGATCCAANTAAAGGTGGCTGTCAATC
AACCCCAGTCGTTTGTGAAGATAAAGGTAAATGTTTAGTTGGTACTTGTCAACCATCCAC
TGGTCAATGTGAATACTCTGATAGAGTTTGTGATAATGGTGGTGCTTTCTGTGTTATTGG
CCAATGTGATCAAAGACTTGGTTGTTTAGTTTTCGATAGAGTTTGTTCATCTGATAATAG
TAGATGTGAAGAAGGTGTTTGTGTAAATGGTACTGAATCTGAAGAAGGTCATTGTAAATC
TGTTAAATATGACCCATTACCATTCAATTGTAATACTGGTGCTGTTGTTTCAACTGCTGT
TATTGCTGGTTCTACTGTCGCTGGTGCTGTTGCTCTTGGTATTTTCCTTTATGGTGGTAA
AAAAGGTTATGACTATTGGAAAGACTCAAGAAATATTTCAATGGGCTCATCAAATAGTAA
TCCATTGTATGAAGAACAACAAACGGAAGAGGTGTCAACCCAATGTAGNACGATCCAG
Length of 3' end seq. 718
Connected seq. ID VHF730P
Connected seq. 
>VHF730P.Seq
TTTACCACTCATCAAAAAACAACAAATAAAAAATAAGAAAGTCTAAAATATTAAAAGAAT
AAAAAAAATATATACAATAAAATAAAAAAACAAAATGTTCATTCAAAGAACATTTTTAAA
AGTTCTTACTCTTTTGAGTATAGTAACAGTTTTGGTCCATGGACAAACTCAACCAAAAGA
TAAAATTACATTGAAGGCCGTAATTTATGATCAACACAAGTTTTATAATCCAAATTTCCA
ACCAAAAAATGAGGGAGAATTTGTTTTAACTAAGGGAATTGTCAAATCAGATATTGATCA
AGAAAAAAGAATTCCAGTTTTAAATAGTATGGATGCCAATGATTCTATAAATAAAAAGGC
AAGAATTAGTTGGCCAGATGGTTTTAAGTATTTTTTTGTAGATAACCAAGCTGGTGATTC
AGAATCTGCCAAATCTGGTAAAAATCTTCCAATTCAAAGAGATATCGAGTTAAATTGGAA
TGGAGAAGCATATGAATATAGTAATTCAAATTACTTCCCAATTAACGGACAANGTTTCAA
TGATGTAAGCTATCCAGTT----------GTTACATGTGGTGGTTGTGAAACNTGTTCAT
ACGCTACCGGTAAATGTGAACCAGATAGTTCACTTTGTAATGATAACAATATTTGTACCA
TTGATATTTGTGTCCATGAAGGTATTCTCGATGGTCTACCACAAGGTAATTGTTCAAATA
CTCCAGTTGATTGTGGTGCCAATGATGAAGATAAATGTAAAACTTGGTCTTGTGATCCAA
NTAAAGGTGGCTGTCAATCAACCCCAGTCGTTTGTGAAGATAAAGGTAAATGTTTAGTTG
GTACTTGTCAACCATCCACTGGTCAATGTGAATACTCTGATAGAGTTTGTGATAATGGTG
GTGCTTTCTGTGTTATTGGCCAATGTGATCAAAGACTTGGTTGTTTAGTTTTCGATAGAG
TTTGTTCATCTGATAATAGTAGATGTGAAGAAGGTGTTTGTGTAAATGGTACTGAATCTG
AAGAAGGTCATTGTAAATCTGTTAAATATGACCCATTACCATTCAATTGTAATACTGGTG
CTGTTGTTTCAACTGCTGTTATTGCTGGTTCTACTGTCGCTGGTGCTGTTGCTCTTGGTA
TTTTCCTTTATGGTGGTAAAAAAGGTTATGACTATTGGAAAGACTCAAGAAATATTTCAA
TGGGCTCATCAAATAGTAATCCATTGTATGAAGAACAACAAACGGAAGAGGTGTCAACCC
AATGTAGNACGATCCAG
Length of connected seq. 1267
Full length Seq ID -
Full length Seq. -
Length of full length seq. -