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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHF649
Library VH
(Link to library)
Clone ID VHF649
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11031-1
Original site URL
Representative seq. ID VHF649P
(Link to Original site)
Representative DNA sequence 
>VHF649 (VHF649Q) /CSM/VH/VHF6-C/VHF649Q.Seq.d/
ATTTAACAACTTGTATGTCATTTATCAATTGTATTATTTCATCACCAAGTGATTTACCCT
CACGTATTGAAATTAGAAAAGCATTTTTAAATTTAAAGATTTTAAAATATATTGAAAATT
TAAGANCAGATTATAATGAAGATAAAAATTTATTAACACAATTAGATGTGTTTGAAGAAG
ANTTATCAACAGATGAACAATTAAATTCACAACAAGGTACACAAATTGGTATTGAAGATT
TATTTTCACAAATTTCATCGCGTGTAACTGGTACACCATCACAACAAGAATTGATAACAT
TGATGACTCATTCCCAACGTATGAGTTCATCAAATTTAAGTTTAAGTGTTTGGACACTCT
ATAATGCATTAGCAAATCAATTGGAAGATGAATTGAAAATTCATCCAGATTTAGATGTTA
CCTTGGTGTCATTGTTATTCCCTGAAGTAAAGAAATCAAGTAGTGGTCTTTTTGGATTTG
GTTCTAAATCCAAATCACCATCATCCTCACCAGCATTATCTTCAATGGCAAAGACTGAAC
TTAAAAAAGATAXXXXXXXXXXTTCAAGATCGTATTCCAGAGATTTGGAATATTGGTGCA
GAGTTACCACATATCGAACATGCTTCAGAGGTAAGTTTAAATAATATCATCAGTGATAGT
AGTGAAATTAAACGTTCCATCGATTTGATTGAACGTGATTTCGTACCAATGATCAATGAT
CCATTGTTTGCTCATGATAAACATTGGATTCACAAAATCACCGAGTTCCAAAAGATCGCC
AAAGTACAATATCAAAGAATTGAAAAGGAAATCGATGAAATGAATAAAGCTTTCGAAGAA
ATCACNTCTTANTCTGGTGAACCAAAATCAACTCAACCCGATGTTTTCTTTTCGACAATT
AATAACTTTTTAGAAGACCTTGAAAAAGCTTACGGTGAATATCAAGCTATGATTAGAAAG
GCTGAATTGGAAAATTCAAAAATGGAAGATCCAGAAAAAGGTGGTCTTCAAGATCTTTCC
TCTCAAATTAGATCTGGTCAATTATTCAAAGATAGAAGAGTTGGTGATTCTGTCATTGCT
CAAATGCAAAATGTTGATAGTTTAAGAAAAAATTTAAAATCAACTTCAACCACAACTCCA
AATACTCCACCAACTATTAAAATTGAATTACCTTCTCAATCAATTTTAAAACCATCAGGC
CAATTAAAAAAATAANATTAAAT
sequence update 2002.10.25
Translated Amino Acid sequence 
LTTCMSFINCIISSPSDLPSRIEIRKAFLNLKILKYIENLRXDYNEDKNLLTQLDVFEEX
LSTDEQLNSQQGTQIGIEDLFSQISSRVTGTPSQQELITLMTHSQRMSSSNLSLSVWTLY
NALANQLEDELKIHPDLDVTLVSLLFPEVKKSSSGLFGFGSKSKSPSSSPALSSMAKTEL
KKD---

---QDRIPEIWNIGAELPHIEHASEVSLNNIISDSSEIKRSIDLIERDFVPMINDPLFAH
DKHWIHKITEFQKIAKVQYQRIEKEIDEMNKAFEEITSXSGEPKSTQPDVFFSTINNFLE
DLEKAYGEYQAMIRKAELENSKMEDPEKGGLQDLSSQIRSGQLFKDRRVGDSVIAQMQNV
DSLRKNLKSTSTTTPNTPPTIKIELPSQSILKPSGQLKK*x*
Translated Amino Acid sequence (All Frames) 
Frame A:
i*qlvchlsivlfhhqviyphvlklekhf*i*rf*nilki*xqiimkikiy*hn*mclkk
xyqqmnn*ihnkvhklvlkiyfhkfhrv*lvhhhnkn**h**lipnv*vhqi*v*vfghs
imh*qinwkmn*kfiqi*mlpwchcyslk*rnqvvvfldlvlnpnhhhphqhylqwqrln
lkki---

---fkivfqrfgilvqsyhisnmlqr*v*iissvivvklnvpsi*lnvisyq*smihcll
minigftkspsskrspkynikelkrksmk*iklskksxlxlvnqnqlnpmfsfrqlitf*
ktlkkltvnikl*lerlnwkiqkwkiqkkvvfkifplkldlvnyskieelvilsllkckm
liv*eki*nqlqpqlqilhqllklnyllnqf*nhqan*knxik

Frame B:
fnnlyviyqlyyfitk*ftlty*n*ksifkfkdfkiy*kfkxrl**r*kfintircv*rr
xinr*tikfttrytnwy*rfiftnfiacnwytittridniddsfptyefikfkfkcldtl
*cisksigr*ienssrfrcylgvivip*skeik*wsfwiwf*iqitiiltsiifngkd*t
*kr---

---srsysrdleywcrvttyrtcfrgkfk*yhq****n*tfhrfd*t*frtndq*sivcs
**tldsqnhrvpkdrqstiskn*kgnr*ne*sfrrnhxlxw*tkinstrcflfdn**lfr
rp*kslr*issyd*kg*igkfkngrsrkrwssrsflsn*iwsiiqr*ksw*fchcsnakc
**fkkkfkinfnhnskystny*n*itfsinfktirpikkixln

Frame C:
LTTCMSFINCIISSPSDLPSRIEIRKAFLNLKILKYIENLRXDYNEDKNLLTQLDVFEEX
LSTDEQLNSQQGTQIGIEDLFSQISSRVTGTPSQQELITLMTHSQRMSSSNLSLSVWTLY
NALANQLEDELKIHPDLDVTLVSLLFPEVKKSSSGLFGFGSKSKSPSSSPALSSMAKTEL
KKD---

---QDRIPEIWNIGAELPHIEHASEVSLNNIISDSSEIKRSIDLIERDFVPMINDPLFAH
DKHWIHKITEFQKIAKVQYQRIEKEIDEMNKAFEEITSXSGEPKSTQPDVFFSTINNFLE
DLEKAYGEYQAMIRKAELENSKMEDPEKGGLQDLSSQIRSGQLFKDRRVGDSVIAQMQNV
DSLRKNLKSTSTTTPNTPPTIKIELPSQSILKPSGQLKK*x*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF649 (VHF649Q) /CSM/VH/VHF6-C/VHF649Q.Seq.d/                       2305   0.0
VHD279 (VHD279Q) /CSM/VH/VHD2-D/VHD279Q.Seq.d/                       1247   0.0
AHN833 (AHN833Q) /CSM/AH/AHN8-B/AHN833Q.Seq.d/                       1247   0.0
AFK767 (AFK767Q) /CSM/AF/AFK7-C/AFK767Q.Seq.d/                       1247   0.0
SHK209 (SHK209Q) /CSM/SH/SHK2-A/SHK209Q.Seq.d/                       1239   0.0
AHN328 (AHN328Q) /CSM/AH/AHN3-B/AHN328Q.Seq.d/                       1225   0.0
VHN829 (VHN829Q) /CSM/VH/VHN8-B/VHN829Q.Seq.d/                       1215   0.0
SHB171 (SHB171Q) /CSM/SH/SHB1-C/SHB171Q.Seq.d/                       1187   0.0
AHI806 (AHI806Q) /CSM/AH/AHI8-A/AHI806Q.Seq.d/                       1187   0.0
AHI721 (AHI721Q) /CSM/AH/AHI7-A/AHI721Q.Seq.d/                       1187   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ440048) Dictyostelium discoideum cDNA clone:ddv42a14, 3' ...  1263   0.0    1
(AJ748258) Dictyostelium discoideum mRNA for diaphanous-rela...  1247   0.0    1
(BJ438433) Dictyostelium discoideum cDNA clone:ddv37m20, 3' ...  1247   0.0    1
(BJ355800) Dictyostelium discoideum cDNA clone:dda58b10, 3' ...  1247   0.0    1
(BJ343275) Dictyostelium discoideum cDNA clone:dda22f17, 3' ...  1247   0.0    1
(BJ407890) Dictyostelium discoideum cDNA clone:dds44a04, 3' ...  1239   0.0    1
(BJ445074) Dictyostelium discoideum cDNA clone:ddv58j08, 3' ...  1164   0.0    3
(BJ403904) Dictyostelium discoideum cDNA clone:dds26m17, 3' ...  1164   0.0    3
(BJ352853) Dictyostelium discoideum cDNA clone:dda48l02, 3' ...  1164   0.0    3
(BJ352847) Dictyostelium discoideum cDNA clone:dda48j05, 3' ...  1164   0.0    3
dna update 2008.10.30
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54N00) RecName: Full=Formin-H; AltName: Full=Diaphanous-relate...   395   e-108
AB061681_1(AB061681|pid:none) Drosophila melanogaster ah1644 mRN...    63   2e-08
AJ008112_1(AJ008112|pid:none) Homo sapiens mRNA for C17orf1 prot...    60   2e-07
BC073988_1(BC073988|pid:none) Homo sapiens formin-like 1, mRNA (...    60   2e-07
AK122266_1(AK122266|pid:none) Mus musculus premature mRNA for mK...    59   3e-07
BC133280_1(BC133280|pid:none) Bos taurus dishevelled associated ...    59   3e-07
BC168411_1(BC168411|pid:none) Synthetic construct Mus musculus c...    59   4e-07
AF056116_14(AF056116|pid:none) Fugu rubripes serine/threonine ki...    59   4e-07
AL845170_3(AL845170|pid:none) Mouse DNA sequence from clone RP23...    59   4e-07
AL845170_2(AL845170|pid:none) Mouse DNA sequence from clone RP23...    59   4e-07
protein update 2009. 7. 4
PSORT 

 psg: 0.94  gvh: 0.55  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.31  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 24.0 %: mitochondrial
	 20.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall
	  4.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for VHF649 is nuc
5' end seq. ID VHF649F
5' end seq. 
>VHF649F.Seq
ATTTAACAACTTGTATGTCATTTATCAATTGTATTATTTCATCACCAAGTGATTTACCCT
CACGTATTGAAATTAGAAAAGCATTTTTAAATTTAAAGATTTTAAAATATATTGAAAATT
TAAGANCAGATTATAATGAAGATAAAAATTTATTAACACAATTAGATGTGTTTGAAGAAG
ANTTATCAACAGATGAACAATTAAATTCACAACAAGGTACACAAATTGGTATTGAAGATT
TATTTTCACAAATTTCATCGCGTGTAACTGGTACACCATCACAACAAGAATTGATAACAT
TGATGACTCATTCCCAACGTATGAGTTCATCAAATTTAAGTTTAAGTGTTTGGACACTCT
ATAATGCATTAGCAAATCAATTGGAAGATGAATTGAAAATTCATCCAGATTTAGATGTTA
CCTTGGTGTCATTGTTATTCCCTGAAGTAAAGAAATCAAGTAGTGGTCTTTTTGGATTTG
GTTCTAAATCCAAATCACCATCATCCTCACCAGCATTATCTTCAATGGCAAAGACTGAAC
TTAAAAAAGATANNNNNNNNNN
Length of 5' end seq. 562
3' end seq. ID VHF649Z
3' end seq. 
>VHF649Z.Seq
NNNNNNNNNNTTCAAGATCGTATTCCAGAGATTTGGAATATTGGTGCAGAGTTACCACAT
ATCGAACATGCTTCAGAGGTAAGTTTAAATAATATCATCAGTGATAGTAGTGAAATTAAA
CGTTCCATCGATTTGATTGAACGTGATTTCGTACCAATGATCAATGATCCATTGTTTGCT
CATGATAAACATTGGATTCACAAAATCACCGAGTTCCAAAAGATCGCCAAAGTACAATAT
CAAAGAATTGAAAAGGAAATCGATGAAATGAATAAAGCTTTCGAAGAAATCACNTCTTAN
TCTGGTGAACCAAAATCAACTCAACCCGATGTTTTCTTTTCGACAATTAATAACTTTTTA
GAAGACCTTGAAAAAGCTTACGGTGAATATCAAGCTATGATTAGAAAGGCTGAATTGGAA
AATTCAAAAATGGAAGATCCAGAAAAAGGTGGTCTTCAAGATCTTTCCTCTCAAATTAGA
TCTGGTCAATTATTCAAAGATAGAAGAGTTGGTGATTCTGTCATTGCTCAAATGCAAAAT
GTTGATAGTTTAAGAAAAAATTTAAAATCAACTTCAACCACAACTCCAAATACTCCACCA
ACTATTAAAATTGAATTACCTTCTCAATCAATTTTAAAACCATCAGGCCAATTAAAAAAA
TAANATTAAAT
Length of 3' end seq. 671
Connected seq. ID VHF649P
Connected seq. 
>VHF649P.Seq
ATTTAACAACTTGTATGTCATTTATCAATTGTATTATTTCATCACCAAGTGATTTACCCT
CACGTATTGAAATTAGAAAAGCATTTTTAAATTTAAAGATTTTAAAATATATTGAAAATT
TAAGANCAGATTATAATGAAGATAAAAATTTATTAACACAATTAGATGTGTTTGAAGAAG
ANTTATCAACAGATGAACAATTAAATTCACAACAAGGTACACAAATTGGTATTGAAGATT
TATTTTCACAAATTTCATCGCGTGTAACTGGTACACCATCACAACAAGAATTGATAACAT
TGATGACTCATTCCCAACGTATGAGTTCATCAAATTTAAGTTTAAGTGTTTGGACACTCT
ATAATGCATTAGCAAATCAATTGGAAGATGAATTGAAAATTCATCCAGATTTAGATGTTA
CCTTGGTGTCATTGTTATTCCCTGAAGTAAAGAAATCAAGTAGTGGTCTTTTTGGATTTG
GTTCTAAATCCAAATCACCATCATCCTCACCAGCATTATCTTCAATGGCAAAGACTGAAC
TTAAAAAAGATA----------TTCAAGATCGTATTCCAGAGATTTGGAATATTGGTGCA
GAGTTACCACATATCGAACATGCTTCAGAGGTAAGTTTAAATAATATCATCAGTGATAGT
AGTGAAATTAAACGTTCCATCGATTTGATTGAACGTGATTTCGTACCAATGATCAATGAT
CCATTGTTTGCTCATGATAAACATTGGATTCACAAAATCACCGAGTTCCAAAAGATCGCC
AAAGTACAATATCAAAGAATTGAAAAGGAAATCGATGAAATGAATAAAGCTTTCGAAGAA
ATCACNTCTTANTCTGGTGAACCAAAATCAACTCAACCCGATGTTTTCTTTTCGACAATT
AATAACTTTTTAGAAGACCTTGAAAAAGCTTACGGTGAATATCAAGCTATGATTAGAAAG
GCTGAATTGGAAAATTCAAAAATGGAAGATCCAGAAAAAGGTGGTCTTCAAGATCTTTCC
TCTCAAATTAGATCTGGTCAATTATTCAAAGATAGAAGAGTTGGTGATTCTGTCATTGCT
CAAATGCAAAATGTTGATAGTTTAAGAAAAAATTTAAAATCAACTTCAACCACAACTCCA
AATACTCCACCAACTATTAAAATTGAATTACCTTCTCAATCAATTTTAAAACCATCAGGC
CAATTAAAAAAATAANATTAAAT
Length of connected seq. 1213
Full length Seq ID -
Full length Seq. -
Length of full length seq. -