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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHF527
Library VH
(Link to library)
Clone ID VHF527
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VHF527P
(Link to Original site)
Representative DNA sequence 
>VHF527 (VHF527Q) /CSM/VH/VHF5-B/VHF527Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGANCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAAGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCXXXXXXXXXXTTGAATTAACTGCCTCCCCAACTATTTTA
GAACCAATTTANTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTACTNT
GTANTCAATCGTCGTANAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTA
TTNTCTGTCAAAGCTCATTTACCAGTANTAGAATCCTTCGGTTTCNCCGATGATTTACGT
TCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTNTTCGATCATTGGGCTTCAATTGGT
GTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGT
TTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTC
GCACTTTGAGTTTTCAAATATAGCAATCAAATCTAAAAAAAATTTTAAAAAAAAAAT
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQXRGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVESVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSF---

---ELTASPTILEPIXLVEITAPENAIGGIYXVXNRRXGIVIGEERRIGSPLXSVKAHLP
VXESFGFXDDLRSHTAGQAFPQCVFDHWASIGVVNKDKKATEVALATRKRKGLAPEIPDL
DKFHEKL*ti*val*vfkysnqi*kkf*kkn
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnkxvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kklisls---

---ln*lppqlf*nqfx**kllpqktllvvftxxsivvxvlllvknvvsvphyxlskliy
qx*npsvspmiyvptlpvklshnvxsiiglqlvlstkirkplksl*pperekv*pqksqi
ltnstknskqfkshfefsniaikskknfkkk

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trxwyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrkclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rslslf---

---*inclpnyfrtnlxsrnycprkrywwyllcxqssxrycyw*rtsyrfpiixcqssft
sxrilrfxr*ftfphcrssfptmcxrslgfnwccqqr*esh*srfshqkekrfsprnprs
*qiprktlnnlsrtlsfqi*qsnlkkilkkk

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQXRGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVESVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSF---

---ELTASPTILEPIXLVEITAPENAIGGIYXVXNRRXGIVIGEERRIGSPLXSVKAHLP
VXESFGFXDDLRSHTAGQAFPQCVFDHWASIGVVNKDKKATEVALATRKRKGLAPEIPDL
DKFHEKL*ti*val*vfkysnqi*kkf*kkn
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF527 (VHF527Q) /CSM/VH/VHF5-B/VHF527Q.Seq.d/                       1818   0.0
CHL163 (CHL163Q) /CSM/CH/CHL1-C/CHL163Q.Seq.d/                       1061   0.0
VHP874 (VHP874Q) /CSM/modify/h12/F/treated/VHP874Q.Seq.d/            1059   0.0
VHH874 (VHH874Q) /CSM/VH/VHH8-D/VHH874Q.Seq.d/                       1059   0.0
VHG874 (VHG874Q) /CSM/VH/VHG8-D/VHG874Q.Seq.d/                       1059   0.0
VHP874 (VHP874Q) /CSM/modify/h12/F/treated/VHP874Q.Seq.d/            1059   0.0
VHH874 (VHH874Q) /CSM/VH/VHH8-D/VHH874Q.Seq.d/                       1059   0.0
VHG874 (VHG874Q) /CSM/VH/VHG8-D/VHG874Q.Seq.d/                       1059   0.0
VHQ476 (VHQ476Q) /CSM/VH/VHQ4-D/VHQ476Q.Seq.d/                       1059   0.0
VHQ473 (VHQ473Q) /CSM/VH/VHQ4-D/VHQ473Q.Seq.d/                       1059   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ421338) Dictyostelium discoideum cDNA clone:ddv42e07, 5' ...  1066   0.0    1
(BJ420226) Dictyostelium discoideum cDNA clone:ddv38j18, 5' ...  1061   0.0    1
(BJ367892) Dictyostelium discoideum cDNA clone:ddc44m15, 5' ...  1061   0.0    1
(BJ427897) Dictyostelium discoideum cDNA clone:ddv63p23, 5' ...  1059   0.0    1
(BJ427892) Dictyostelium discoideum cDNA clone:ddv63o22, 5' ...  1059   0.0    1
(BJ427891) Dictyostelium discoideum cDNA clone:ddv63o21, 5' ...  1059   0.0    1
(BJ427889) Dictyostelium discoideum cDNA clone:ddv63o19, 5' ...  1059   0.0    1
(BJ427888) Dictyostelium discoideum cDNA clone:ddv63n23, 5' ...  1059   0.0    1
(BJ427875) Dictyostelium discoideum cDNA clone:ddv63k24, 5' ...  1059   0.0    1
(BJ427874) Dictyostelium discoideum cDNA clone:ddv63k23, 5' ...  1059   0.0    1
dna update 2008.10.29
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM270011_29(AM270011|pid:none) Aspergillus niger contig An02c016...   245   2e-63
DQ357217_1(DQ357217|pid:none) Leishmania braziliensis elongation...   241   3e-62
AM494972_26(AM494972|pid:none) Leishmania braziliensis chromosom...   241   3e-62
AF248644_1(AF248644|pid:none) Filobasidiella neoformans var. neo...   241   3e-62
AF083031_87(AF083031|pid:none) Guillardia theta nucleomorph chro...   241   4e-62
AY618550_1(AY618550|pid:none) Trypanosoma cruzi elongation facto...   241   4e-62
CT005272_19(CT005272|pid:none) Leishmania major strain Friedlin,...   241   4e-62
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   238   3e-61
(Q6FYA7) RecName: Full=Elongation factor 2;          Short=EF-2;...   238   3e-61
(Q874B9) RecName: Full=Elongation factor 2;          Short=EF-2;...   237   4e-61
protein update 2009. 7. 4
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.35  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHF527 is cyt
5' end seq. ID VHF527F
5' end seq. 
>VHF527F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGANCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAAGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCNNNNNNNNNN
Length of 5' end seq. 571
3' end seq. ID VHF527Z
3' end seq. 
>VHF527Z.Seq
NNNNNNNNNNTTGAATTAACTGCCTCCCCAACTATTTTAGAACCAATTTANTTAGTAGAA
ATTACTGCCCCAGAAAACGCTATTGGTGGTATTTACTNTGTANTCAATCGTCGTANAGGT
ATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTNTCTGTCAAAGCTCATTTA
CCAGTANTAGAATCCTTCGGTTTCNCCGATGATTTACGTTCCCACACTGCCGGTCAAGCT
TTCCCACAATGTGTNTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAA
GCCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGAT
CTTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTTGAGTTTTCAAATAT
AGCAATCAAATCTAAAAAAAATTTTAAAAAAAAAAT
Length of 3' end seq. 456
Connected seq. ID VHF527P
Connected seq. 
>VHF527P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGANCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAAGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCC----------TTGAATTAACTGCCTCCCCAACTATTTTA
GAACCAATTTANTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTACTNT
GTANTCAATCGTCGTANAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTA
TTNTCTGTCAAAGCTCATTTACCAGTANTAGAATCCTTCGGTTTCNCCGATGATTTACGT
TCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTNTTCGATCATTGGGCTTCAATTGGT
GTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGT
TTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTC
GCACTTTGAGTTTTCAAATATAGCAATCAAATCTAAAAAAAATTTTAAAAAAAAAAT
Length of connected seq. 1007
Full length Seq ID -
Full length Seq. -
Length of full length seq. -