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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHF510
Library VH
(Link to library)
Clone ID VHF510
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15722-1
Original site URL
Representative seq. ID VHF510P
(Link to Original site)
Representative DNA sequence 
>VHF510 (VHF510Q) /CSM/VH/VHF5-A/VHF510Q.Seq.d/
AAAAAATAAAGGAAAAAACACACCAAAATTAAATAAGTAAGAATAAAAATGAAATATTTA
TTAGTATATTTACACATATTACTATTTTGTTTATTAAAGATCAATAGCCAAAAGTATTGC
ACTTATAGNGGTGTTGGAGAAGATTATAATATCAAATTTGATGTCTTACAAACAAATGGN
GGTATTGGTTTTCTATACTCATATTTAAGTGTTATTTTTACAACTTGTAATTATACTATT
CCTTGTGGTAGTAATAGTAATAATGTATTGNGTGGATCATATCATTCAACTGTTCCACGC
AAGTATCTTTCATTGGGATTTGTTAATGGCAGTACACATAGTTATAGCAAAGACGGACTA
ATGATAACATATATATCACAGGATACAACAAATGATAATGATTGCAAAAGATATACAACA
AATGTGATTTTTCAATGTXXXXXXXXXXTGTACATGCCCATAATGACCTTTTGGTAANGG
TTGTGAATTTACTCGAGGTCCACTCGATTGTTCGACACCAAATGGTACTTGTGATAATAA
TACTGGTAATTGTACATGCCATAATGAACATTTTGGTAATTCTTGTGAATTTACTCGATG
TCCACTCGATTGTTCGACACCAAATGGTACTTGTGATAATAATACTGGTAATTGTACATG
CCATAATGAACATTTTGGTAATGGTTGTGAATTCACTCAATGCCCACTCTATTGTTCAAC
ACCAAATGGTACTTGTGATATTAATAGTGGTATTTGTACATGTGATAATGAACATATTGG
TAATGGTTGCGAAATTAAATTTATTGAATGTAAACATAAATGTTCAACCNAACATGGAAT
ATGTGATAATGATAGTGGTAATTGTAAATGTGATACTCAAACAAAAGGATTAACGTGTGA
AGAATCAAGATTATTAATTGAATCATTAGACTCAATCAATTCAAAAGGTGGTACAATAAA
TATAATTGGTTATTTTGGAAATACAACATCATTATTAACAATTAAAATTGGTGAATCACA
ATGTAAAAATATCAAAGTATTCAATGAAACAACATTAAAATGTGATATTGGTGAAGGTAA
AGGTATTCATAATATTACAATCATTGATGATGACNTTTCGTTCACAGCAATCAATAAGTT
CCAATATCTCGATGAAAAAACAAAAATCTCAAAAATATCGAGAGGTGCNATTGCTGGTAT
TGTTGTTGGTTGTGTTGTTGCTGTTTCATTAATTGGTTCTATTGCTTTTTATTATCATCA
CAAAAATAAAAAGGTTAATCTTTTCT
sequence update 2002.10.25
Translated Amino Acid sequence 
kk*rkkhtkik*VRIKMKYLLVYLHILLFCLLKINSQKYCTYXGVGEDYNIKFDVLQTNG
GIGFLYSYLSVIFTTCNYTIPCGSNSNNVLXGSYHSTVPRKYLSLGFVNGSTHSYSKDGL
MITYISQDTTNDNDCKRYTTNVIFQC---

---VHAHNDLLVXVVNLLEVHSIVRHQMVLVIIILVIVHAIMNILVILVNLLDVHSIVRH
QMVLVIIILVIVHAIMNILVMVVNSLNAHSIVQHQMVLVILIVVFVHVIMNILVMVAKLN
LLNVNINVQPNMEYVIMIVVIVNVILKQKD*rvknqdy*lnh*tqsiqkvvq*i*lvile
iqhhy*qlklvnhnvkiskysmkqh*nvilvkvkvfiilqslmmtfrsqqsissnismkk
qksqkyrevxllvlllvvlllfh*lvlllfiiitkikrlifs
Translated Amino Acid sequence (All Frames) 
Frame A:
kk*rkkhtkik*VRIKMKYLLVYLHILLFCLLKINSQKYCTYXGVGEDYNIKFDVLQTNG
GIGFLYSYLSVIFTTCNYTIPCGSNSNNVLXGSYHSTVPRKYLSLGFVNGSTHSYSKDGL
MITYISQDTTNDNDCKRYTTNVIFQC---

---ctcp**pfgxgceftrgpldcstpngtcdnntgnctchnehfgnsceftrcpldcst
pngtcdnntgnctchnehfgngceftqcplycstpngtcdinsgictcdnehigngceik
fieckhkcstxhgicdndsgnckcdtqtkgltceesrlliesldsinskggtiniigyfg
nttslltikigesqcknikvfnettlkcdigegkgihnitiidddxsftainkfqyldek
tkiskisrgaiagivvgcvvavsligsiafyyhhknkkvnlf

Frame B:
knkgkntpklnk*e*k*niy*yiytyyyfvy*rsiaksialixvlekiiisnlmsykqmx
vlvfythi*vlflqlviilflvvvivimyxvdhiiqlfhasifhwdllmavhiviaktd*
**hiyhriqqmimiakdiqqm*ffn---

---VHAHNDLLVXVVNLLEVHSIVRHQMVLVIIILVIVHAIMNILVILVNLLDVHSIVRH
QMVLVIIILVIVHAIMNILVMVVNSLNAHSIVQHQMVLVILIVVFVHVIMNILVMVAKLN
LLNVNINVQPNMEYVIMIVVIVNVILKQKD*rvknqdy*lnh*tqsiqkvvq*i*lvile
iqhhy*qlklvnhnvkiskysmkqh*nvilvkvkvfiilqslmmtfrsqqsissnismkk
qksqkyrevxllvlllvvlllfh*lvlllfiiitkikrlifs

Frame C:
kikekthqn*isknkneifisifthitilfikdq*pkvlhl*xcwrrl*yqi*cltnkwx
ywfsilifkcyfynl*lyyslw*****cixwiisfncstqvsfigic*wqyt*l*qrrtn
dniyitgynk***lqkiynkcdfsm---

---ympimtfw*xl*iysrstrlfdtkwyl***yw*lymp**tfw*fl*iysmstrlfdt
kwyl***yw*lymp**tfw*wl*ihsmptllfntkwyl*y**wylym***tyw*wlrn*i
y*m*t*mfnxtwnm****w*l*m*ysnkrinv*rikiin*iirlnqfkrwynkynwlfwk
yniiinn*nw*itm*kyqsiq*nnikm*yw*r*rys*yynh***xfvhsnq*vpisr*kn
knlkniercxcwyccwlccccfinwfycfllssqk*kg*sf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF510 (VHF510Q) /CSM/VH/VHF5-A/VHF510Q.Seq.d/                       2083   0.0
VHP829 (VHP829Q) /CSM/VH/VHP8-B/VHP829Q.Seq.d/                       1342   0.0
VHH737 (VHH737Q) /CSM/VH/VHH7-B/VHH737Q.Seq.d/                       1340   0.0
VHF630 (VHF630Q) /CSM/VH/VHF6-B/VHF630Q.Seq.d/                       1340   0.0
VHQ484 (VHQ484Q) /CSM/VH/VHQ4-D/VHQ484Q.Seq.d/                       1336   0.0
VHQ143 (VHQ143Q) /CSM/VH/VHQ1-B/VHQ143Q.Seq.d/                       1336   0.0
VHO766 (VHO766Q) /CSM/VH/VHO7-C/VHO766Q.Seq.d/                       1336   0.0
VHI141 (VHI141Q) /CSM/VH/VHI1-B/VHI141Q.Seq.d/                       1336   0.0
FC-BE20 (FC-BE20Q) /CSM/FC/FC-BE/FC-BE20Q.Seq.d/                     1336   0.0
AHO674 (AHO674Q) /CSM/AH/AHO6-D/AHO674Q.Seq.d/                       1336   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ439904) Dictyostelium discoideum cDNA clone:ddv42c03, 3' ...  1382   0.0    2
(BJ440018) Dictyostelium discoideum cDNA clone:ddv42k08, 3' ...  1340   0.0    2
(AU284609) Dictyostelium discoideum gamete cDNA clone:FC-BE2...  1334   0.0    2
(BJ446789) Dictyostelium discoideum cDNA clone:ddv63h22, 3' ...  1334   0.0    2
(BJ446619) Dictyostelium discoideum cDNA clone:ddv63e11, 3' ...  1334   0.0    2
(BJ446052) Dictyostelium discoideum cDNA clone:ddv61a18, 3' ...  1334   0.0    2
(BJ443076) Dictyostelium discoideum cDNA clone:ddv51g23, 3' ...  1334   0.0    2
(BJ441604) Dictyostelium discoideum cDNA clone:ddv47a11, 3' ...  1334   0.0    2
(BJ441328) Dictyostelium discoideum cDNA clone:ddv46j09, 3' ...  1334   0.0    2
(BJ445753) Dictyostelium discoideum cDNA clone:ddv60d17, 3' ...  1332   0.0    2
dna update 2008.10.29
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC117075_64(AC117075|pid:none) Dictyostelium discoideum chromoso...   124   6e-27
AC116100_9(AC116100|pid:none) Dictyostelium discoideum chromosom...   107   1e-21
AC116920_20(AC116920|pid:none) Dictyostelium discoideum chromoso...    96   4e-18
AC116967_7(AC116967|pid:none) Dictyostelium discoideum chromosom...    94   1e-17
AC116920_24(AC116920|pid:none) Dictyostelium discoideum chromoso...    93   2e-17
AC116963_42(AC116963|pid:none) Dictyostelium discoideum chromoso...    92   3e-17
AC116920_25(AC116920|pid:none) Dictyostelium discoideum chromoso...    89   3e-16
AC116967_8(AC116967|pid:none) Dictyostelium discoideum chromosom...    88   6e-16
AC117081_39(AC117081|pid:none) Dictyostelium discoideum chromoso...    88   8e-16
AC116956_34(AC116956|pid:none) Dictyostelium discoideum chromoso...    84   8e-15
protein update 2009. 7. 4
PSORT 

 psg: 0.93  gvh: 0.70  alm: 0.12  top: 0.40  tms: 0.27  mit: 0.31  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 40.0 %: endoplasmic reticulum
	 24.0 %: plasma membrane
	 20.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: nuclear
	  4.0 %: cytoplasmic

>> prediction for VHF510 is end
5' end seq. ID VHF510F
5' end seq. 
>VHF510F.Seq
AAAAAATAAAGGAAAAAACACACCAAAATTAAATAAGTAAGAATAAAAATGAAATATTTA
TTAGTATATTTACACATATTACTATTTTGTTTATTAAAGATCAATAGCCAAAAGTATTGC
ACTTATAGNGGTGTTGGAGAAGATTATAATATCAAATTTGATGTCTTACAAACAAATGGN
GGTATTGGTTTTCTATACTCATATTTAAGTGTTATTTTTACAACTTGTAATTATACTATT
CCTTGTGGTAGTAATAGTAATAATGTATTGNGTGGATCATATCATTCAACTGTTCCACGC
AAGTATCTTTCATTGGGATTTGTTAATGGCAGTACACATAGTTATAGCAAAGACGGACTA
ATGATAACATATATATCACAGGATACAACAAATGATAATGATTGCAAAAGATATACAACA
AATGTGATTTTTCAATGTNNNNNNNNNN
Length of 5' end seq. 448
3' end seq. ID VHF510Z
3' end seq. 
>VHF510Z.Seq
NNNNNNNNNNTGTACATGCCCATAATGACCTTTTGGTAANGGTTGTGAATTTACTCGAGG
TCCACTCGATTGTTCGACACCAAATGGTACTTGTGATAATAATACTGGTAATTGTACATG
CCATAATGAACATTTTGGTAATTCTTGTGAATTTACTCGATGTCCACTCGATTGTTCGAC
ACCAAATGGTACTTGTGATAATAATACTGGTAATTGTACATGCCATAATGAACATTTTGG
TAATGGTTGTGAATTCACTCAATGCCCACTCTATTGTTCAACACCAAATGGTACTTGTGA
TATTAATAGTGGTATTTGTACATGTGATAATGAACATATTGGTAATGGTTGCGAAATTAA
ATTTATTGAATGTAAACATAAATGTTCAACCNAACATGGAATATGTGATAATGATAGTGG
TAATTGTAAATGTGATACTCAAACAAAAGGATTAACGTGTGAAGAATCAAGATTATTAAT
TGAATCATTAGACTCAATCAATTCAAAAGGTGGTACAATAAATATAATTGGTTATTTTGG
AAATACAACATCATTATTAACAATTAAAATTGGTGAATCACAATGTAAAAATATCAAAGT
ATTCAATGAAACAACATTAAAATGTGATATTGGTGAAGGTAAAGGTATTCATAATATTAC
AATCATTGATGATGACNTTTCGTTCACAGCAATCAATAAGTTCCAATATCTCGATGAAAA
AACAAAAATCTCAAAAATATCGAGAGGTGCNATTGCTGGTATTGTTGTTGGTTGTGTTGT
TGCTGTTTCATTAATTGGTTCTATTGCTTTTTATTATCATCACAAAAATAAAAAGGTTAA
TCTTTTCT
Length of 3' end seq. 848
Connected seq. ID VHF510P
Connected seq. 
>VHF510P.Seq
AAAAAATAAAGGAAAAAACACACCAAAATTAAATAAGTAAGAATAAAAATGAAATATTTA
TTAGTATATTTACACATATTACTATTTTGTTTATTAAAGATCAATAGCCAAAAGTATTGC
ACTTATAGNGGTGTTGGAGAAGATTATAATATCAAATTTGATGTCTTACAAACAAATGGN
GGTATTGGTTTTCTATACTCATATTTAAGTGTTATTTTTACAACTTGTAATTATACTATT
CCTTGTGGTAGTAATAGTAATAATGTATTGNGTGGATCATATCATTCAACTGTTCCACGC
AAGTATCTTTCATTGGGATTTGTTAATGGCAGTACACATAGTTATAGCAAAGACGGACTA
ATGATAACATATATATCACAGGATACAACAAATGATAATGATTGCAAAAGATATACAACA
AATGTGATTTTTCAATGT----------TGTACATGCCCATAATGACCTTTTGGTAANGG
TTGTGAATTTACTCGAGGTCCACTCGATTGTTCGACACCAAATGGTACTTGTGATAATAA
TACTGGTAATTGTACATGCCATAATGAACATTTTGGTAATTCTTGTGAATTTACTCGATG
TCCACTCGATTGTTCGACACCAAATGGTACTTGTGATAATAATACTGGTAATTGTACATG
CCATAATGAACATTTTGGTAATGGTTGTGAATTCACTCAATGCCCACTCTATTGTTCAAC
ACCAAATGGTACTTGTGATATTAATAGTGGTATTTGTACATGTGATAATGAACATATTGG
TAATGGTTGCGAAATTAAATTTATTGAATGTAAACATAAATGTTCAACCNAACATGGAAT
ATGTGATAATGATAGTGGTAATTGTAAATGTGATACTCAAACAAAAGGATTAACGTGTGA
AGAATCAAGATTATTAATTGAATCATTAGACTCAATCAATTCAAAAGGTGGTACAATAAA
TATAATTGGTTATTTTGGAAATACAACATCATTATTAACAATTAAAATTGGTGAATCACA
ATGTAAAAATATCAAAGTATTCAATGAAACAACATTAAAATGTGATATTGGTGAAGGTAA
AGGTATTCATAATATTACAATCATTGATGATGACNTTTCGTTCACAGCAATCAATAAGTT
CCAATATCTCGATGAAAAAACAAAAATCTCAAAAATATCGAGAGGTGCNATTGCTGGTAT
TGTTGTTGGTTGTGTTGTTGCTGTTTCATTAATTGGTTCTATTGCTTTTTATTATCATCA
CAAAAATAAAAAGGTTAATCTTTTCT
Length of connected seq. 1276
Full length Seq ID -
Full length Seq. -
Length of full length seq. -