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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHF494
Library VH
(Link to library)
Clone ID VHF494
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11753-1|Contig-U13565-1
Original site URL
Representative seq. ID VHF494P
(Link to Original site)
Representative DNA sequence 
>VHF494 (VHF494Q) /CSM/VH/VHF4-D/VHF494Q.Seq.d/
TTAATCGTTGTGATGAAATTGAAAATCAACGTATTCAAAGAGTTTTACTCGAAAAGAAAG
AAAAAGAAACAACCGATGATTTGGAAAAATCAAAAATCGGTGAACAAATTAAAAAATTAC
CAACTTTCCCTTACTATGCTTCATTAACAATTGAAAATCAATATGATGAAACAATTTATG
ATGCCGCTGAACAATTGATGAGCTTCCTCTTGGATAATATTTCATTCATTGATGAGAAAT
TAAGAAAATCTAAACTTTTCAATATTAAAAACATTGAAGATGAACAATTAATTAAAGATA
GAGAAGAAACCGAGAAATTAGTTACTGAATCATTATCAAATTGTCTTAGATTTGTTAGAT
CACCATCATTGGTTTGTAAATTTGCAAATGCTTTAGGAGCAAAGAGAGAATCATTATATT
TAGAGATTTCAAAACATATTCATCCAATTATCATTGAAAAACATAAACAAGCAATGGAAA
TTAAACAACATCAATTCCTTATTGAAACTTTAAATTTAGAATTATCTGATCTCTCXXXXX
XXXXXATTCANATTTTCCCACATCCATCATCTGATAATGATCAAGAGTTGGATATTCAAT
TGNATCTTTTAGTTAGTCTTTATGATGCTGTAGATCAACATGCTTTCCATTTAATTAATG
GTGTAATTCCAATCATTCAACGTTTCGCTAAAAGATGTTATATCGAATGGAAATATGAAA
AATTAGATATTCTCTATGATTCACTTCAAAAGAGATTCCCAAAGGAAATCATTTCAATCT
TTACCATTGCCATTGATATGATGTTGGTTAATATCTTACAAAGTCAATATCCATTGTTTT
TAAATATGATGAAATCAATGAAAAATCGTCTTGTTGCAACACTTGGTCTAGATGGTCTTT
GGGAAGATTTCTTGGCTGGTTTTAGAAAGAATTATAAATCAAAAAAGAGACTCATTCAAA
TGATTTCTTTAATTGGTGATTCTGTTTGGCAAATTAATGTAAATCCAAATAGTGCCNCTG
GTAAACAAATTGCAAAAAGAACTCTCCCNCAATCATCACAATCTGTTCATAAAAAGATTA
AAGTTTCTGCTCCAANTCTTTCACTTACTGGTGTACCAAAAAAATTATCTGNTAAAAAAT
CTGTTGACGATGATGAAGAATTTGATGAAAATGAAAATGAAAATGGATATGAAGATGAAA
ATGAAAATGAAAATGGA
sequence update 2002.10.25
Translated Amino Acid sequence 
NRCDEIENQRIQRVLLEKKEKETTDDLEKSKIGEQIKKLPTFPYYASLTIENQYDETIYD
AAEQLMSFLLDNISFIDEKLRKSKLFNIKNIEDEQLIKDREETEKLVTESLSNCLRFVRS
PSLVCKFANALGAKRESLYLEISKHIHPIIIEKHKQAMEIKQHQFLIETLNLELSDL---

---IXIFPHPSSDNDQELDIQLXLLVSLYDAVDQHAFHLINGVIPIIQRFAKRCYIEWKY
EKLDILYDSLQKRFPKEIISIFTIAIDMMLVNILQSQYPLFLNMMKSMKNRLVATLGLDG
LWEDFLAGFRKNYKSKKRLIQMISLIGDSVWQINVNPNSAXGKQIAKRTLPQSSQSVHKK
IKVSAPXLSLTGVPKKLSXKKSVDDDEEFDENENENGYEDENENENG
Translated Amino Acid sequence (All Frames) 
Frame A:
livvmklkinvfkefyskrkkkkqpmiwknqksvnklknyqlsltmlh*qlkinmmkqfm
mplnn**asswiifhslmrn*enlnfsilktlkmnn*lkiekkprn*llnhyqivldlld
hhhwfvnlqml*eqrenhyi*rfqnifiqlslkninkqwklnninsllkl*i*nylis--
-

---IXIFPHPSSDNDQELDIQLXLLVSLYDAVDQHAFHLINGVIPIIQRFAKRCYIEWKY
EKLDILYDSLQKRFPKEIISIFTIAIDMMLVNILQSQYPLFLNMMKSMKNRLVATLGLDG
LWEDFLAGFRKNYKSKKRLIQMISLIGDSVWQINVNPNSAXGKQIAKRTLPQSSQSVHKK
IKVSAPXLSLTGVPKKLSXKKSVDDDEEFDENENENGYEDENENENG

Frame B:
*sl**n*kstysksftrkerkrnnr*fgkiknr*tn*kitnfpllcfinn*ksi**nnl*
cr*tidelplg*yfih**eikki*tfqy*kh*r*tin*r*rrnreisy*iiikls*ic*i
tiigl*ickcfrskeriiifrdfktyssnyh*kt*tsngn*ttsipy*nfkfrii*sl--
-

---fxfshihhlimikswifnxif*lvfmml*inmlsi*lmv*fqsfnvslkdvisngnm
kn*ifsmihfkrdsqrksfqslplpli*cwlisykvnihcf*i**nq*kivllqhlv*mv
fgkiswlvleriinqkrdsfk*fl*lvilfgklm*iqivplvnklqkelsxnhhnlfikr
lkfllqxfhllvyqknylxknlltmmknlmkmkmkmdmkmkmkmkm

Frame C:
NRCDEIENQRIQRVLLEKKEKETTDDLEKSKIGEQIKKLPTFPYYASLTIENQYDETIYD
AAEQLMSFLLDNISFIDEKLRKSKLFNIKNIEDEQLIKDREETEKLVTESLSNCLRFVRS
PSLVCKFANALGAKRESLYLEISKHIHPIIIEKHKQAMEIKQHQFLIETLNLELSDL---

---sxfptsii***srvgysixsfs*sl*ccrstcfpfn*wcnsnhstfr*kmlyrmei*
kirysl*ftskeipkgnhfnlyhch*ydvg*yltksisivfkydeinekssccntwsrws
lgrflgwf*kel*ikkethsndffnw*fclan*cksk*cxw*tnckknspxiitics*kd
*sfcsxsftywctkkiix*kic*r**ri**k*k*kwi*r*k*k*kw
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF494 (VHF494Q) /CSM/VH/VHF4-D/VHF494Q.Seq.d/                       1893   0.0
VHO174 (VHO174Q) /CSM/VH/VHO1-D/VHO174Q.Seq.d/                       1096   0.0
VHD626 (VHD626Q) /CSM/VH/VHD6-B/VHD626Q.Seq.d/                       1096   0.0
VHG869 (VHG869Q) /CSM/VH/VHG8-C/VHG869Q.Seq.d/                        959   0.0
VHC140 (VHC140Q) /CSM/VH/VHC1-B/VHC140Q.Seq.d/                        952   0.0
SSM755 (SSM755Q) /CSM/SS/SSM7-C/SSM755Q.Seq.d/                         40   0.078
SSG471 (SSG471Q) /CSM/SS/SSG4-C/SSG471Q.Seq.d/                         40   0.078
SLE425 (SLE425Q) /CSM/SL/SLE4-B/SLE425Q.Seq.d/                         40   0.078
AFD695 (AFD695Q) /CSM/AF/AFD6-D/AFD695Q.Seq.d/                         40   0.078
AFC739 (AFC739Q) /CSM/AF/AFC7-B/AFC739Q.Seq.d/                         40   0.078
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ439871) Dictyostelium discoideum cDNA clone:ddv41l24, 3' ...  1104   0.0    1
(BJ445531) Dictyostelium discoideum cDNA clone:ddv59c19, 3' ...  1096   0.0    1
(BJ438717) Dictyostelium discoideum cDNA clone:ddv38c08, 3' ...  1096   0.0    1
(BJ440755) Dictyostelium discoideum cDNA clone:ddv44j18, 3' ...   959   0.0    1
(BJ437811) Dictyostelium discoideum cDNA clone:ddv35o09, 3' ...   952   0.0    1
(BJ421160) Dictyostelium discoideum cDNA clone:ddv41l24, 5' ...   829   0.0    2
(BJ426650) Dictyostelium discoideum cDNA clone:ddv59c19, 5' ...   100   2e-16  1
(BJ422039) Dictyostelium discoideum cDNA clone:ddv44j18, 5' ...    74   1e-08  1
(AC116984) Dictyostelium discoideum chromosome 2 map 2567470...    42   0.018  14
(AW333432) S21G1 AGS-1 Pneumocystis carinii cDNA 3', mRNA se...    50   0.20   1
dna update 2008.10.29
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP000361_498(CP000361|pid:none) Arcobacter butzleri RM4018, comp...    39   0.29
(Q46189) Uncharacterized protein in hydrogenase 1 5'region (Frag...    37   1.1
CP000962_338(CP000962|pid:none) Clostridium botulinum A3 str. Lo...    35   4.1
CP001083_336(CP001083|pid:none) Clostridium botulinum Ba4 str. 6...    35   5.4
DQ489736_1460(DQ489736|pid:none) Leuconostoc citreum KM20, compl...    35   5.4
AM412317_335(AM412317|pid:none) Clostridium botulinum A str. ATC...    35   5.4
CP000728_334(CP000728|pid:none) Clostridium botulinum F str. Lan...    35   5.4
FM992693_372(FM992693|pid:none) Candida dubliniensis CD36 chromo...    34   9.2
FM992693_384(FM992693|pid:none) Candida dubliniensis CD36 chromo...    34   9.2
protein update 2009. 7. 4
PSORT 

 psg: 0.75  gvh: 0.17  alm: 0.25  top: 0.47  tms: 0.07  mit: 0.23  mip: 0.02
 nuc: 0.06  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.06  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 32.0 %: mitochondrial
	 20.0 %: nuclear
	 16.0 %: cytoplasmic
	 12.0 %: Golgi
	 12.0 %: endoplasmic reticulum
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane

>> prediction for VHF494 is mit
5' end seq. ID VHF494F
5' end seq. 
>VHF494F.Seq
TTAATCGTTGTGATGAAATTGAAAATCAACGTATTCAAAGAGTTTTACTCGAAAAGAAAG
AAAAAGAAACAACCGATGATTTGGAAAAATCAAAAATCGGTGAACAAATTAAAAAATTAC
CAACTTTCCCTTACTATGCTTCATTAACAATTGAAAATCAATATGATGAAACAATTTATG
ATGCCGCTGAACAATTGATGAGCTTCCTCTTGGATAATATTTCATTCATTGATGAGAAAT
TAAGAAAATCTAAACTTTTCAATATTAAAAACATTGAAGATGAACAATTAATTAAAGATA
GAGAAGAAACCGAGAAATTAGTTACTGAATCATTATCAAATTGTCTTAGATTTGTTAGAT
CACCATCATTGGTTTGTAAATTTGCAAATGCTTTAGGAGCAAAGAGAGAATCATTATATT
TAGAGATTTCAAAACATATTCATCCAATTATCATTGAAAAACATAAACAAGCAATGGAAA
TTAAACAACATCAATTCCTTATTGAAACTTTAAATTTAGAATTATCTGATCTCTCNNNNN
NNNNN
Length of 5' end seq. 545
3' end seq. ID VHF494Z
3' end seq. 
>VHF494Z.Seq
NNNNNNNNNNATTCANATTTTCCCACATCCATCATCTGATAATGATCAAGAGTTGGATAT
TCAATTGNATCTTTTAGTTAGTCTTTATGATGCTGTAGATCAACATGCTTTCCATTTAAT
TAATGGTGTAATTCCAATCATTCAACGTTTCGCTAAAAGATGTTATATCGAATGGAAATA
TGAAAAATTAGATATTCTCTATGATTCACTTCAAAAGAGATTCCCAAAGGAAATCATTTC
AATCTTTACCATTGCCATTGATATGATGTTGGTTAATATCTTACAAAGTCAATATCCATT
GTTTTTAAATATGATGAAATCAATGAAAAATCGTCTTGTTGCAACACTTGGTCTAGATGG
TCTTTGGGAAGATTTCTTGGCTGGTTTTAGAAAGAATTATAAATCAAAAAAGAGACTCAT
TCAAATGATTTCTTTAATTGGTGATTCTGTTTGGCAAATTAATGTAAATCCAAATAGTGC
CNCTGGTAAACAAATTGCAAAAAGAACTCTCCCNCAATCATCACAATCTGTTCATAAAAA
GATTAAAGTTTCTGCTCCAANTCTTTCACTTACTGGTGTACCAAAAAAATTATCTGNTAA
AAAATCTGTTGACGATGATGAAGAATTTGATGAAAATGAAAATGAAAATGGATATGAAGA
TGAAAATGAAAATGAAAATGGA
Length of 3' end seq. 682
Connected seq. ID VHF494P
Connected seq. 
>VHF494P.Seq
TTAATCGTTGTGATGAAATTGAAAATCAACGTATTCAAAGAGTTTTACTCGAAAAGAAAG
AAAAAGAAACAACCGATGATTTGGAAAAATCAAAAATCGGTGAACAAATTAAAAAATTAC
CAACTTTCCCTTACTATGCTTCATTAACAATTGAAAATCAATATGATGAAACAATTTATG
ATGCCGCTGAACAATTGATGAGCTTCCTCTTGGATAATATTTCATTCATTGATGAGAAAT
TAAGAAAATCTAAACTTTTCAATATTAAAAACATTGAAGATGAACAATTAATTAAAGATA
GAGAAGAAACCGAGAAATTAGTTACTGAATCATTATCAAATTGTCTTAGATTTGTTAGAT
CACCATCATTGGTTTGTAAATTTGCAAATGCTTTAGGAGCAAAGAGAGAATCATTATATT
TAGAGATTTCAAAACATATTCATCCAATTATCATTGAAAAACATAAACAAGCAATGGAAA
TTAAACAACATCAATTCCTTATTGAAACTTTAAATTTAGAATTATCTGATCTCTC-----
-----ATTCANATTTTCCCACATCCATCATCTGATAATGATCAAGAGTTGGATATTCAAT
TGNATCTTTTAGTTAGTCTTTATGATGCTGTAGATCAACATGCTTTCCATTTAATTAATG
GTGTAATTCCAATCATTCAACGTTTCGCTAAAAGATGTTATATCGAATGGAAATATGAAA
AATTAGATATTCTCTATGATTCACTTCAAAAGAGATTCCCAAAGGAAATCATTTCAATCT
TTACCATTGCCATTGATATGATGTTGGTTAATATCTTACAAAGTCAATATCCATTGTTTT
TAAATATGATGAAATCAATGAAAAATCGTCTTGTTGCAACACTTGGTCTAGATGGTCTTT
GGGAAGATTTCTTGGCTGGTTTTAGAAAGAATTATAAATCAAAAAAGAGACTCATTCAAA
TGATTTCTTTAATTGGTGATTCTGTTTGGCAAATTAATGTAAATCCAAATAGTGCCNCTG
GTAAACAAATTGCAAAAAGAACTCTCCCNCAATCATCACAATCTGTTCATAAAAAGATTA
AAGTTTCTGCTCCAANTCTTTCACTTACTGGTGTACCAAAAAAATTATCTGNTAAAAAAT
CTGTTGACGATGATGAAGAATTTGATGAAAATGAAAATGAAAATGGATATGAAGATGAAA
ATGAAAATGAAAATGGA
Length of connected seq. 1207
Full length Seq ID -
Full length Seq. -
Length of full length seq. -