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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHF491
Library VH
(Link to library)
Clone ID VHF491
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11249-1|Contig-U12738-1
Original site URL
Representative seq. ID VHF491P
(Link to Original site)
Representative DNA sequence 
>VHF491 (VHF491Q) /CSM/VH/VHF4-D/VHF491Q.Seq.d/
AAATAATATAATTATATTAACAAAAAAATATAATAGGAATCAAAAATATTTAGAGAATTG
ATTCAAAATGCAGAAGATGCTCGAGCTGATACAGTCATAATAAAGGTTGATGAAGGCTCA
TATCCAAATAATGGGTTATTAATTTCAAATGATAACCAAGAAATTCAAAGTTCAAAGGAA
TTATTAGGTCCATCAATTTTAATTTATAATAATGCAACTTTTCAAAAACATGATTGGGAA
GGAATTTGTCAAATTAGTCAAGGTAGTAAAAAAGAAAATCTAAAAAGTGTTGGCAATTTC
GGTTTAGGTTGGAATAGTGTTTATCATATAACAGATAATCCAATTGTTTATAGTGGTAAT
AATGTTTGGTTTGCAGATCCAAATGAGAGAATGAGTGGTGGTTTATTCTTTGATTTGGAT
GATGAAGATGATTATAACAAATGTCTTAGTTTTTTAGAGCCATTCATTCAATTTAAAGAA
TCAAATTGTAACCCAAAAGAATATTTTAATGGTACAATCATTCGTCTACCATTGXXXXXX
XXXXAAAATTTTAAATCAATCTTTGGATGGTTTAACTGGTTATTTATCTAAATTTGGCTT
AAAATTTAAACTATCAGATAAACATATATGTTCAAAGGTTATTGATTTTCTTTCAAATCA
AGATTCAATAGAAACNTATAAAAATTTTATGGATTATTTAAATCAAAGAGGTGAGAATAT
TGGTGATTCATTAATTGATTTCAAAGATATCCCATTTATTCCATCCAGTGAATTCGATAC
NTATGATGGATTTGGCTCAAATGAATCTAAAATTTCATTATCAGAGTGTACACTATCAAG
TTATAAAGATTGTTTATTCNCTGTAAAAACTCCAATTAAAGTAA
sequence update 2002.10.25
Translated Amino Acid sequence 
k*ynyinkki**ESKIFRELIQNAEDARADTVIIKVDEGSYPNNGLLISNDNQEIQSSKE
LLGPSILIYNNATFQKHDWEGICQISQGSKKENLKSVGNFGLGWNSVYHITDNPIVYSGN
NVWFADPNERMSGGLFFDLDDEDDYNKCLSFLEPFIQFKESNCNPKEYFNGTIIRLPL--
-

---KILNQSLDGLTGYLSKFGLKFKLSDKHICSKVIDFLSNQDSIETYKNFMDYLNQRGE
NIGDSLIDFKDIPFIPSSEFDTYDGFGSNESKISLSECTLSSYKDCLFXVKTPIKV
Translated Amino Acid sequence (All Frames) 
Frame A:
k*ynyinkki**ESKIFRELIQNAEDARADTVIIKVDEGSYPNNGLLISNDNQEIQSSKE
LLGPSILIYNNATFQKHDWEGICQISQGSKKENLKSVGNFGLGWNSVYHITDNPIVYSGN
NVWFADPNERMSGGLFFDLDDEDDYNKCLSFLEPFIQFKESNCNPKEYFNGTIIRLPL--
-

---KILNQSLDGLTGYLSKFGLKFKLSDKHICSKVIDFLSNQDSIETYKNFMDYLNQRGE
NIGDSLIDFKDIPFIPSSEFDTYDGFGSNESKISLSECTLSSYKDCLFXVKTPIKV

Frame B:
nniiiltkkynrnqkylen*fkmqkmleliqs**rlmkahiqimgy*fqmitkkfkvqrn
y*vhqf*fiimqlfknmigkefvklvkvvkkki*kvlaisv*vgivfii*qiiqlfivvi
mfglqiqmre*vvvysliwmmkmiitnvlvf*shsfnlknqivtqknilmvqsfvyh---

---kf*inlwmv*lviylnla*nlnyqiniyvqrlliffqikiq*kxikilwii*ikevr
ilvih*liskishlfhpvnsixmmdlaqmnlkfhyqsvhyqvikivysl*klqlk*

Frame C:
ii*ly*qkniigikni*ridskcrrcss*yshnkg**rlisk*wvinfk**prnskfkgi
irsinfnl**cnfskt*lgrnlsn*sr**krkskkcwqfrfrle*clsynr*sncl*w**
clvcrsk*enewwfil*fg**r*l*qms*ffraihsi*rikl*pkrif*wynhssti---

---nfksifgwfnwlfi*iwlki*tir*tymfkgy*fsfksrfnrnx*kfyglfkskr*e
yw*fin*fqrypiysiq*iryx*wiwlk*i*nfiirvytikl*rlfixcknsn*s
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF491 (VHF491Q) /CSM/VH/VHF4-D/VHF491Q.Seq.d/                       1108   0.0
VHO533 (VHO533Q) /CSM/VH/VHO5-B/VHO533Q.Seq.d/                        656   0.0
AHJ527 (AHJ527Q) /CSM/AH/AHJ5-B/AHJ527Q.Seq.d/                        587   e-166
SHI292 (SHI292Q) /CSM/SH/SHI2-D/SHI292Q.Seq.d/                        450   e-125
VHN647 (VHN647Q) /CSM/VH/VHN6-B/VHN647Q.Seq.d/                        426   e-118
VHK810 (VHK810Q) /CSM/VH/VHK8-A/VHK810Q.Seq.d/                        426   e-118
VHK791 (VHK791Q) /CSM/VH/VHK7-D/VHK791Q.Seq.d/                        426   e-118
VHE271 (VHE271Q) /CSM/VH/VHE2-C/VHE271Q.Seq.d/                        426   e-118
VHD239 (VHD239Q) /CSM/VH/VHD2-B/VHD239Q.Seq.d/                        426   e-118
VHC102 (VHC102Q) /CSM/VH/VHC1-A/VHC102Q.Seq.d/                        426   e-118
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ421132) Dictyostelium discoideum cDNA clone:ddv41f24, 5' ...   490   0.0    3
(BJ419850) Dictyostelium discoideum cDNA clone:ddv37m10, 5' ...   426   0.0    2
(BJ338795) Dictyostelium discoideum cDNA clone:dda63g06, 5' ...   426   0.0    2
(BJ418948) Dictyostelium discoideum cDNA clone:ddv34f17, 5' ...   426   0.0    2
(BJ426269) Dictyostelium discoideum cDNA clone:ddv58m12, 5' ...   426   0.0    2
(BJ395962) Dictyostelium discoideum cDNA clone:dds40g24, 5' ...   450   0.0    2
(BJ420557) Dictyostelium discoideum cDNA clone:ddv39m18, 5' ...   426   0.0    2
(BJ424548) Dictyostelium discoideum cDNA clone:ddv52f23, 5' ...   426   0.0    2
(BJ420400) Dictyostelium discoideum cDNA clone:ddv39m05, 5' ...   418   0.0    2
(BJ426718) Dictyostelium discoideum cDNA clone:ddv60b04, 5' ...   418   0.0    2
dna update 2008.10.29
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BC142916_1(BC142916|pid:none) Danio rerio hypothetical LOC555303...    97   6e-19
(Q9NZJ4) RecName: Full=Sacsin;  &AL157766_4(AL157766|pid:none)         97   9e-19
BC171956_1(BC171956|pid:none) Mus musculus sacsin, mRNA (cDNA cl...    96   1e-18
BC138482_1(BC138482|pid:none) Mus musculus sacsin, mRNA (cDNA cl...    96   2e-18
(Q9JLC8) RecName: Full=Sacsin;                                         96   2e-18
CP001577_292(CP001577|pid:none) Micromonas sp. RCC299 chromosome...    89   2e-16
AL732351_6(AL732351|pid:none) Oryza sativa genomic DNA, chromoso...    87   7e-16
AL606457_18(AL606457|pid:none) Oryza sativa genomic DNA, chromos...    87   7e-16
AB006708_10(AB006708|pid:none) Arabidopsis thaliana genomic DNA,...    84   6e-15
CR749427_1(CR749427|pid:none) Homo sapiens mRNA; cDNA DKFZp686B1...    71   4e-11
protein update 2009. 7. 4
PSORT 

 psg: 0.58  gvh: 0.39  alm: 0.51  top: 0.53  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 32.0 %: nuclear
	 20.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VHF491 is cyt
5' end seq. ID VHF491F
5' end seq. 
>VHF491F.Seq
AAATAATATAATTATATTAACAAAAAAATATAATAGGAATCAAAAATATTTAGAGAATTG
ATTCAAAATGCAGAAGATGCTCGAGCTGATACAGTCATAATAAAGGTTGATGAAGGCTCA
TATCCAAATAATGGGTTATTAATTTCAAATGATAACCAAGAAATTCAAAGTTCAAAGGAA
TTATTAGGTCCATCAATTTTAATTTATAATAATGCAACTTTTCAAAAACATGATTGGGAA
GGAATTTGTCAAATTAGTCAAGGTAGTAAAAAAGAAAATCTAAAAAGTGTTGGCAATTTC
GGTTTAGGTTGGAATAGTGTTTATCATATAACAGATAATCCAATTGTTTATAGTGGTAAT
AATGTTTGGTTTGCAGATCCAAATGAGAGAATGAGTGGTGGTTTATTCTTTGATTTGGAT
GATGAAGATGATTATAACAAATGTCTTAGTTTTTTAGAGCCATTCATTCAATTTAAAGAA
TCAAATTGTAACCCAAAAGAATATTTTAATGGTACAATCATTCGTCTACCATTGNNNNNN
NNNN
Length of 5' end seq. 544
3' end seq. ID VHF491Z
3' end seq. 
>VHF491Z.Seq
NNNNNNNNNNAAAATTTTAAATCAATCTTTGGATGGTTTAACTGGTTATTTATCTAAATT
TGGCTTAAAATTTAAACTATCAGATAAACATATATGTTCAAAGGTTATTGATTTTCTTTC
AAATCAAGATTCAATAGAAACNTATAAAAATTTTATGGATTATTTAAATCAAAGAGGTGA
GAATATTGGTGATTCATTAATTGATTTCAAAGATATCCCATTTATTCCATCCAGTGAATT
CGATACNTATGATGGATTTGGCTCAAATGAATCTAAAATTTCATTATCAGAGTGTACACT
ATCAAGTTATAAAGATTGTTTATTCNCTGTAAAAACTCCAATTAAAGTAA
Length of 3' end seq. 350
Connected seq. ID VHF491P
Connected seq. 
>VHF491P.Seq
AAATAATATAATTATATTAACAAAAAAATATAATAGGAATCAAAAATATTTAGAGAATTG
ATTCAAAATGCAGAAGATGCTCGAGCTGATACAGTCATAATAAAGGTTGATGAAGGCTCA
TATCCAAATAATGGGTTATTAATTTCAAATGATAACCAAGAAATTCAAAGTTCAAAGGAA
TTATTAGGTCCATCAATTTTAATTTATAATAATGCAACTTTTCAAAAACATGATTGGGAA
GGAATTTGTCAAATTAGTCAAGGTAGTAAAAAAGAAAATCTAAAAAGTGTTGGCAATTTC
GGTTTAGGTTGGAATAGTGTTTATCATATAACAGATAATCCAATTGTTTATAGTGGTAAT
AATGTTTGGTTTGCAGATCCAAATGAGAGAATGAGTGGTGGTTTATTCTTTGATTTGGAT
GATGAAGATGATTATAACAAATGTCTTAGTTTTTTAGAGCCATTCATTCAATTTAAAGAA
TCAAATTGTAACCCAAAAGAATATTTTAATGGTACAATCATTCGTCTACCATTG------
----AAAATTTTAAATCAATCTTTGGATGGTTTAACTGGTTATTTATCTAAATTTGGCTT
AAAATTTAAACTATCAGATAAACATATATGTTCAAAGGTTATTGATTTTCTTTCAAATCA
AGATTCAATAGAAACNTATAAAAATTTTATGGATTATTTAAATCAAAGAGGTGAGAATAT
TGGTGATTCATTAATTGATTTCAAAGATATCCCATTTATTCCATCCAGTGAATTCGATAC
NTATGATGGATTTGGCTCAAATGAATCTAAAATTTCATTATCAGAGTGTACACTATCAAG
TTATAAAGATTGTTTATTCNCTGTAAAAACTCCAATTAAAGTAA
Length of connected seq. 874
Full length Seq ID -
Full length Seq. -
Length of full length seq. -