NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHF489
Library VH
(Link to library)
Clone ID VHF489
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VHF489P
(Link to Original site)
Representative DNA sequence 
>VHF489 (VHF489Q) /CSM/VH/VHF4-D/VHF489Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCAGTTC
AGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCGTCGA
AGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCAAACC
AGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTGAAGA
AGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACACTGA
AGATXXXXXXXXXXGATGAATTAACTGCCTCCCCAAATATCTTAGAACCAATCTACTTAG
TAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTANTCTGTACTCAATCGTCGTA
GAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTATCTGTCAAAGCTC
ATTTACCAGTATTAGAATCNTTCGGTTTCACCGATGATTTACGTTCCCACACTGCCGGTC
AAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATA
AGAAAGCCNCTGAAGTCGCTTTAGCCNCCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCC
CAGATCTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTGAGTTTTCAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*IFICITSLRNAKGRQITSWLYFTRIINLIDSPGHVDFSS
EVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTEE
AYLSFRRAIESVNVIVGNTED---

---DELTASPNILEPIYLVEITAPENAIGGIXSVLNRRRGIVIGEERRIGSPLLSVKAHL
PVLESFGFTDDLRSHTAGQAFPQCVFDHWASIGVVNKDKKAXEVALAXRKRKGLAPEIPD
LTNSTKNSKQFKSHLSFQ
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnnqsyrfprsr*fqf
rsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*r
slslfp*sy*irqchcw*h*r---

---DELTASPNILEPIYLVEITAPENAIGGIXSVLNRRRGIVIGEERRIGSPLLSVKAHL
PVLESFGFTDDLRSHTAGQAFPQCVFDHWASIGVVNKDKKAXEVALAXRKRKGLAPEIPD
LTNSTKNSKQFKSHLSFQ

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*IFICITSLRNAKGRQITSWLYFTRIINLIDSPGHVDFSS
EVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTEE
AYLSFRRAIESVNVIVGNTED---

---mn*lppqis*nqst**kllpqktllvvfxlysivvevlllvknvvsvphyylskliy
qy*nxsvspmiyvptlpvklshnvssiiglqlvlstkirkplksl*pperekv*pqksqi
*qiprktlnnlsrt*vfk

Frame C:
lssakkvkykmvnftidqiraimdrrenirnmsviahvdhgkttlsdsliqragiiadkv
sgdmrymscradeqergitiksssvslhfempkedklpagctshe*silsipqvtlisvq
k*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tlkk
lislsvellnpsmsllvtlk---

---*inclpkylrtnllsrnycprkrywwylxctqss*rycyw*rtsyrfpiiicqssft
sirixrfhr*ftfphcrssfptmclrslgfnwccqqr*esx*srfsxqkekrfsprnprs
dkfhekl*ti*valefs
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF489 (VHF489Q) /CSM/VH/VHF4-D/VHF489Q.Seq.d/                       1899   0.0
VHG874 (VHG874Q) /CSM/VH/VHG8-D/VHG874Q.Seq.d/                       1132   0.0
VHQ476 (VHQ476Q) /CSM/VH/VHQ4-D/VHQ476Q.Seq.d/                       1132   0.0
VHQ473 (VHQ473Q) /CSM/VH/VHQ4-D/VHQ473Q.Seq.d/                       1132   0.0
VHQ469 (VHQ469Q) /CSM/VH/VHQ4-C/VHQ469Q.Seq.d/                       1132   0.0
VHQ463 (VHQ463Q) /CSM/VH/VHQ4-C/VHQ463Q.Seq.d/                       1132   0.0
VHQ453 (VHQ453Q) /CSM/VH/VHQ4-C/VHQ453Q.Seq.d/                       1132   0.0
VHQ446 (VHQ446Q) /CSM/VH/VHQ4-B/VHQ446Q.Seq.d/                       1132   0.0
VHQ441 (VHQ441Q) /CSM/VH/VHQ4-B/VHQ441Q.Seq.d/                       1132   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ421114) Dictyostelium discoideum cDNA clone:ddv41b24, 5' ...  1158   0.0    1
(M26017) D.discoideum elongation factor 2 mRNA, complete cds.     609   0.0    2
(BJ427897) Dictyostelium discoideum cDNA clone:ddv63p23, 5' ...   591   0.0    2
(BJ427892) Dictyostelium discoideum cDNA clone:ddv63o22, 5' ...   591   0.0    2
(BJ427891) Dictyostelium discoideum cDNA clone:ddv63o21, 5' ...   591   0.0    2
(BJ427889) Dictyostelium discoideum cDNA clone:ddv63o19, 5' ...   591   0.0    2
(BJ427888) Dictyostelium discoideum cDNA clone:ddv63n23, 5' ...   591   0.0    2
(BJ427882) Dictyostelium discoideum cDNA clone:ddv63m22, 5' ...   591   0.0    2
(BJ427875) Dictyostelium discoideum cDNA clone:ddv63k24, 5' ...   591   0.0    2
(BJ427874) Dictyostelium discoideum cDNA clone:ddv63k23, 5' ...   591   0.0    2
dna update 2008.10.29
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM270011_29(AM270011|pid:none) Aspergillus niger contig An02c016...   149   7e-64
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   150   2e-63
S05988(S05988) translation elongation factor eEF-2 - fruit fly (...   152   2e-63
A40411(A40411) translation elongation factor eEF-2 - Caenorhabdi...   147   4e-63
AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   148   8e-63
AF248644_1(AF248644|pid:none) Filobasidiella neoformans var. neo...   149   8e-63
AY026074_1(AY026074|pid:none) Monosiga brevicollis elongation fa...   147   1e-62
(Q874B9) RecName: Full=Elongation factor 2;          Short=EF-2;...   149   1e-62
(Q1HPK6) RecName: Full=Translation elongation factor 2;         ...   152   2e-62
AY078407_1(AY078407|pid:none) Spodoptera exigua translation elon...   152   2e-62
protein update 2009. 7. 4
PSORT 

 psg: 0.51  gvh: 0.62  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.39  mip: 0.05
 nuc: 0.04  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: cytoplasmic
	 28.0 %: nuclear
	 24.0 %: mitochondrial
	  8.0 %: vesicles of secretory system
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHF489 is cyt
5' end seq. ID VHF489F
5' end seq. 
>VHF489F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCAGTTC
AGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCGTCGA
AGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCAAACC
AGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTGAAGA
AGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACACTGA
AGATNNNNNNNNNN
Length of 5' end seq. 614
3' end seq. ID VHF489Z
3' end seq. 
>VHF489Z.Seq
NNNNNNNNNNGATGAATTAACTGCCTCCCCAAATATCTTAGAACCAATCTACTTAGTAGA
AATTACTGCCCCAGAAAACGCTATTGGTGGTATTTANTCTGTACTCAATCGTCGTAGAGG
TATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTATCTGTCAAAGCTCATTT
ACCAGTATTAGAATCNTTCGGTTTCACCGATGATTTACGTTCCCACACTGCCGGTCAAGC
TTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAA
AGCCNCTGAAGTCGCTTTAGCCNCCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGA
TCTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTGAGTTTTCAAA
Length of 3' end seq. 416
Connected seq. ID VHF489P
Connected seq. 
>VHF489P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCAGTTC
AGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCGTCGA
AGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCAAACC
AGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTGAAGA
AGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACACTGA
AGAT----------GATGAATTAACTGCCTCCCCAAATATCTTAGAACCAATCTACTTAG
TAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTANTCTGTACTCAATCGTCGTA
GAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTATCTGTCAAAGCTC
ATTTACCAGTATTAGAATCNTTCGGTTTCACCGATGATTTACGTTCCCACACTGCCGGTC
AAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATA
AGAAAGCCNCTGAAGTCGCTTTAGCCNCCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCC
CAGATCTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTGAGTTTTCAAA
Length of connected seq. 1010
Full length Seq ID -
Full length Seq. -
Length of full length seq. -