>VHF475 (VHF475Q) /CSM/VH/VHF4-D/VHF475Q.Seq.d/
XXXXXXXXXXTGGCATCGTTCAAAGATTACCTNTATCGCCGATGGTAAAATCAATGTTAA
TCTCATTGATTTGGGTGAAAGAGAATCTTTCCCACAATCTCAATCAAGTACTCTCATTAG
AAATATCAATCATAAATTACAAAGCCTTCCTTCATTGGTAACTTTGGTCAAATTAGCNTC
TTGTAAGAATCNTTCCAATGATGATATNTCCAATGATGCTATGGATTTCATGGAAAAAGA
GTTTTTAGATCTCAAAGTTGGCGTTAATATCATACGTGATATCGATGGTACTCAACATGT
TCTCNTNTCTGATAATGCATGGTATTATCAATGGTGAATTGGTTCGTAATGGTTTGGTTA
GTGTCGATCGTTCAAGTAAATTACCNTCAATTCAACAACTTCAAGATGAAGAACAAAAAG
CTAAATCAAAGAGATTAGGTGTTTGGAGATTCGGTGATATCGATAGTGATGATGAAGATG
ATAAACCACGTTCAAATTTCAAAGGTAAAGGTGGTAAACCNATTTCAAAAAAATAATTAT
AATAATTTATAAATTTTCTTTTTATTTAAATAAATAAATAAATAAATAAAAAAGG

---HRSKITXIADGKINVNLIDLGERESFPQSQSSTLIRNINHKLQSLPSLVTLVKLASC
KNXSNDDXSNDAMDFMEKEFLDLKVGVNIIRDIDGTQHVLXSDNAWYYQW*igs*wfg*c
rsfk*itxnsttsr*rtks*ikeircleir*yr****r**ttfkfqr*rw*txfkkiiii
iykfsfylnk*ink*kr

Frame A:
---givqrlpxspmvksmlisliwvkenlshnlnqvlsleisiinykaflhw*lwsn*xl
vrixpmmixpmmlwiswkksf*isklalisyvismvlnmfsxlimhgiingelvrnglvs
vdrssklpsiqqlqdeeqkakskrlgvwrfgdidsddeddkprsnfkgkggkpiskk*l*
*finflfi*ink*inkk


Frame B:
---asfkdylyrrw*nqc*sh*fg*krifptisikysh*kyqs*itkpsfignfgqisxl
*esfq**yxq*cygfhgkrvfrsqswr*yht*yrwystcsxx**cmvlsmvnwfvmvwlv
sivqvnyxqfnnfkmknkklnqrd*vfgdsvisivmmkminhvqiskvkvvnxfqknnyn
nl*ifflfk*ink*ikk


Frame C:
---HRSKITXIADGKINVNLIDLGERESFPQSQSSTLIRNINHKLQSLPSLVTLVKLASC
KNXSNDDXSNDAMDFMEKEFLDLKVGVNIIRDIDGTQHVLXSDNAWYYQW*igs*wfg*c
rsfk*itxnsttsr*rtks*ikeircleir*yr****r**ttfkfqr*rw*txfkkiiii
iykfsfylnk*ink*kr

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF475 (VHF475Q) /CSM/VH/VHF4-D/VHF475Q.Seq.d/                        975   0.0
VHQ162 (VHQ162Q) /CSM/VH/VHQ1-C/VHQ162Q.Seq.d/                        944   0.0
VHP896 (VHP896Q) /CSM/VH/VHP8-D/VHP896Q.Seq.d/                        944   0.0
VHN113 (VHN113Q) /CSM/VH/VHN1-A/VHN113Q.Seq.d/                        944   0.0
VHL165 (VHL165Q) /CSM/VH/VHL1-C/VHL165Q.Seq.d/                        944   0.0
VHJ886 (VHJ886Q) /CSM/VH/VHJ8-D/VHJ886Q.Seq.d/                        944   0.0
VHJ403 (VHJ403Q) /CSM/VH/VHJ4-A/VHJ403Q.Seq.d/                        944   0.0
VHB524 (VHB524Q) /CSM/VH/VHB5-A/VHB524Q.Seq.d/                        944   0.0
SHL548 (SHL548Q) /CSM/SH/SHL5-B/SHL548Q.Seq.d/                        944   0.0
SHK270 (SHK270Q) /CSM/SH/SHK2-C/SHK270Q.Seq.d/                        944   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ439842) Dictyostelium discoideum cDNA clone:ddv41f20, 3' ...   975   0.0    1
(BJ437419) Dictyostelium discoideum cDNA clone:ddv34o05, 3' ...   567   0.0    2
(BJ446518) Dictyostelium discoideum cDNA clone:ddv62p24, 3' ...   567   0.0    2
(BJ348957) Dictyostelium discoideum cDNA clone:dda34l23, 3' ...   567   0.0    2
(BJ408935) Dictyostelium discoideum cDNA clone:dds47o11, 3' ...   567   0.0    2
(BJ382953) Dictyostelium discoideum cDNA clone:ddc46l07, 3' ...   567   0.0    2
(BJ351992) Dictyostelium discoideum cDNA clone:dda45d12, 3' ...   567   0.0    2
(BJ380989) Dictyostelium discoideum cDNA clone:ddc39p23, 3' ...   567   0.0    2
(BJ355745) Dictyostelium discoideum cDNA clone:dda58g04, 3' ...   567   0.0    2
(BJ348606) Dictyostelium discoideum cDNA clone:dda33c20, 3' ...   567   0.0    2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP001330_515(CP001330|pid:none) Micromonas sp. RCC299 chromosome...    54   4e-06
AY320036_1(AY320036|pid:none) Oryza sativa (japonica cultivar-gr...    48   2e-04
AE014186_355(AE014186|pid:none) Plasmodium falciparum 3D7 chromo...    46   7e-04
BT055838_1(BT055838|pid:none) Zea mays full-length cDNA clone ZM...    46   9e-04
BT071103_1(BT071103|pid:none) Picea sitchensis clone WS02771_D01...    46   9e-04
AM398207_1(AM398207|pid:none) Picea abies mRNA for TUDOR protein...    46   9e-04
AB078603_1(AB078603|pid:none) Pisum sativum SN4TDR mRNA for 110 ...    44   0.003
AB055904_1(AB055904|pid:none) Pisum sativum SN4TDR mRNA for 110 ...    44   0.003
AB010069_10(AB010069|pid:none) Arabidopsis thaliana genomic DNA,...    44   0.004
CR382133_106(CR382133|pid:none) Debaryomyces hansenii strain CBS...    43   0.006

 psg: 0.69  gvh: 0.62  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.20  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 28.0 %: nuclear
	 16.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VHF475 is cyt

>VHF475Z.Seq
NNNNNNNNNNTGGCATCGTTCAAAGATTACCTNTATCGCCGATGGTAAAATCAATGTTAA
TCTCATTGATTTGGGTGAAAGAGAATCTTTCCCACAATCTCAATCAAGTACTCTCATTAG
AAATATCAATCATAAATTACAAAGCCTTCCTTCATTGGTAACTTTGGTCAAATTAGCNTC
TTGTAAGAATCNTTCCAATGATGATATNTCCAATGATGCTATGGATTTCATGGAAAAAGA
GTTTTTAGATCTCAAAGTTGGCGTTAATATCATACGTGATATCGATGGTACTCAACATGT
TCTCNTNTCTGATAATGCATGGTATTATCAATGGTGAATTGGTTCGTAATGGTTTGGTTA
GTGTCGATCGTTCAAGTAAATTACCNTCAATTCAACAACTTCAAGATGAAGAACAAAAAG
CTAAATCAAAGAGATTAGGTGTTTGGAGATTCGGTGATATCGATAGTGATGATGAAGATG
ATAAACCACGTTCAAATTTCAAAGGTAAAGGTGGTAAACCNATTTCAAAAAAATAATTAT
AATAATTTATAAATTTTCTTTTTATTTAAATAAATAAATAAATAAATAAAAAAGG