NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHF449
Library VH
(Link to library)
Clone ID VHF449
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14527-1
Original site URL
Representative seq. ID VHF449P
(Link to Original site)
Representative DNA sequence 
>VHF449 (VHF449Q) /CSM/VH/VHF4-C/VHF449Q.Seq.d/
TTGGCCTACTGGAAAATTAAATAATTTTTTTTTATATATAATGGAACAAAAAGAATTTGA
TATTAAAAATATTTTATTGAAGATTGAAAAAGATAAAAATGAAACTTCAAAAAAAATACA
AGAAAAATATAAAGATTGTACAATTGAAGATTTGGAAAAATCTTTAAGAATCCAAAATAA
AAAATCAGAAGAAATTACTTTTATCTCAAAAAAGATTGAAGAATTAAATGAAAAATTAAT
AGTTGAAAAATATAAACCATTATTGTCATCGTCCAATAATTCAAATGAAATTGAAAATGA
GATATCACCAAAAATTAAAGAAATTTTAATTAAAAACTCTATATTAAAAAATGAAAATTC
AGCAGTATCACCATCGATAACAACAACAGATGCATCAGCATCATTAAGTTTTGGGGGTAA
AACAATTGTTTTACCATCAAATCAACCAACAACATTAAGAAAGAGTGATGCACCAGCTGA
TACAAAACATATTAAATTGGAAATTACCAATGATAATAAAATTAATATTATTTGGCAGGA
TGCAAAAAATTATCAATCAAAAGATTGGGTTGCACTTTATAACTATXXXXXXXXXXGAAT
CNATTTTAAAATTTTTAAAATTTAGAATTAATGNACCNAAAAGAAAAAGTTTTCCANGTT
TGNCAAGCNANTTTTACNANTGNANTTTTTTANGAANTTTTTGGACANGANTTAANAACT
TGGNCTGTANAACATTGTAANAAANTCAANGAANTTTTTAAAGATNTTGAAACCTTTGNT
T
sequence update 2002.10.25
Translated Amino Acid sequence 
WPTGKLNNFFLYIMEQKEFDIKNILLKIEKDKNETSKKIQEKYKDCTIEDLEKSLRIQNK
KSEEITFISKKIEELNEKLIVEKYKPLLSSSNNSNEIENEISPKIKEILIKNSILKNENS
AVSPSITTTDASASLSFGGKTIVLPSNQPTTLRKSDAPADTKHIKLEITNDNKINIIWQD
AKNYQSKDWVALYNY---

---ESILKFLKFRINXPKRKSFPXLXSXFYXXXFLXXFWTXXXNLXCXTL*xxqxxf*rx
*nlx
Translated Amino Acid sequence (All Frames) 
Frame A:
laywkik*fffiyngtkri*y*kyfied*kr*k*nfkkntrki*rlyn*rfgkifknpk*
kirrnyfylkkd*rik*kins*ki*tiivivq*fk*n*k*ditkn*rnfn*klyikk*kf
ssitidnnnrcisiikfwg*nncftikstnnikke*cts*ykty*ignyq***n*yylag
ckklsikrlgctl*l---

---ESILKFLKFRINXPKRKSFPXLXSXFYXXXFLXXFWTXXXNLXCXTL*xxqxxf*rx
*nlx

Frame B:
WPTGKLNNFFLYIMEQKEFDIKNILLKIEKDKNETSKKIQEKYKDCTIEDLEKSLRIQNK
KSEEITFISKKIEELNEKLIVEKYKPLLSSSNNSNEIENEISPKIKEILIKNSILKNENS
AVSPSITTTDASASLSFGGKTIVLPSNQPTTLRKSDAPADTKHIKLEITNDNKINIIWQD
AKNYQSKDWVALYNY---

---nxf*nf*nlelmxxkekvfxvxqaxftxxxfxexfgxxlxtwxvxhcxkxxexfkdx
etfx

Frame C:
gllen*iiffyi*wnkknlilkify*rlkkikmklqkkykknikivqlkiwknl*eskik
nqkklllsqkrlkn*mkn**lkninhychrpiiqmklkmryhqklkkf*lktly*kmkiq
qyhhr*qqqmhqhh*vlgvkqlfyhqinqqh*ervmhqliqnilnwklpmiiklilfgrm
qkiinqkiglhfit---

---ixfkifki*n*xtxkkkfsxfxkxxlxxxffxxfldxx*xlglxnivxxsxxflkxl
kplx
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF449 (VHF449Q) /CSM/VH/VHF4-C/VHF449Q.Seq.d/                        634   0.0
VHO505 (VHO505Q) /CSM/VH/VHO5-A/VHO505Q.Seq.d/                        450   e-125
VHN166 (VHN166Q) /CSM/VH/VHN1-C/VHN166Q.Seq.d/                        450   e-125
VHJ324 (VHJ324Q) /CSM/VH/VHJ3-A/VHJ324Q.Seq.d/                        450   e-125
VHF474 (VHF474Q) /CSM/VH/VHF4-D/VHF474Q.Seq.d/                        450   e-125
VHD531 (VHD531Q) /CSM/VH/VHD5-B/VHD531Q.Seq.d/                        450   e-125
VHB385 (VHB385Q) /CSM/VH/VHB3-D/VHB385Q.Seq.d/                        450   e-125
VHA187 (VHA187Q) /CSM/VH/VHA1-D/VHA187Q.Seq.d/                        450   e-125
SSG273 (SSG273Q) /CSM/SS/SSG2-D/SSG273Q.Seq.d/                        450   e-125
VHI804 (VHI804Q) /CSM/VH/VHI8-A/VHI804Q.Seq.d/                        440   e-122
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ421036) Dictyostelium discoideum cDNA clone:ddv41b14, 5' ...   450   e-140  5
(BJ423444) Dictyostelium discoideum cDNA clone:ddv49p05, 5' ...   450   e-132  4
(BJ418180) Dictyostelium discoideum cDNA clone:ddv31m21, 5' ...   450   e-132  4
(BJ425922) Dictyostelium discoideum cDNA clone:ddv57c17, 5' ...   450   e-132  4
(BJ418750) Dictyostelium discoideum cDNA clone:ddv33j21, 5' ...   450   e-132  4
(BJ426743) Dictyostelium discoideum cDNA clone:ddv60i01, 5' ...   450   e-132  4
(BJ420157) Dictyostelium discoideum cDNA clone:ddv38m07, 5' ...   450   e-130  3
(BJ423108) Dictyostelium discoideum cDNA clone:ddv48h02, 5' ...   440   e-129  4
(BJ420745) Dictyostelium discoideum cDNA clone:ddv40e11, 5' ...   428   e-125  4
(BJ425591) Dictyostelium discoideum cDNA clone:ddv56a07, 5' ...   428   e-125  4
dna update 2008.10.29
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54BH5) RecName: Full=PI-PLC X-box domain-containing protein DD...   305   1e-81
CP000993_302(CP000993|pid:none) Borrelia recurrentis A1, complet...    43   0.010
AM910994_58(AM910994|pid:none) Plasmodium knowlesi strain H chro...    42   0.022
AL162272_2(AL162272|pid:none) Human DNA sequence from clone RP11...    40   0.065
(Q9UPS8) RecName: Full=Ankyrin repeat domain-containing protein ...    40   0.065
BC130453_1(BC130453|pid:none) Homo sapiens ankyrin repeat domain...    40   0.065
BC150737_1(BC150737|pid:none) Mus musculus myosin, heavy polypep...    40   0.11
(P13542) RecName: Full=Myosin-8; AltName: Full=Myosin heavy chai...    40   0.11
(P13535) RecName: Full=Myosin-8; AltName: Full=Myosin heavy chai...    39   0.25
M36769_1(M36769|pid:none) Homo sapiens perinatal myosin heavy ch...    39   0.25
protein update 2009. 7. 4
PSORT 

 psg: 0.82  gvh: 0.04  alm: 0.50  top: 0.53  tms: 0.00  mit: 0.15  mip: 0.00
 nuc: 0.09  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 24.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: Golgi

>> prediction for VHF449 is nuc
5' end seq. ID VHF449F
5' end seq. 
>VHF449F.Seq
TTGGCCTACTGGAAAATTAAATAATTTTTTTTTATATATAATGGAACAAAAAGAATTTGA
TATTAAAAATATTTTATTGAAGATTGAAAAAGATAAAAATGAAACTTCAAAAAAAATACA
AGAAAAATATAAAGATTGTACAATTGAAGATTTGGAAAAATCTTTAAGAATCCAAAATAA
AAAATCAGAAGAAATTACTTTTATCTCAAAAAAGATTGAAGAATTAAATGAAAAATTAAT
AGTTGAAAAATATAAACCATTATTGTCATCGTCCAATAATTCAAATGAAATTGAAAATGA
GATATCACCAAAAATTAAAGAAATTTTAATTAAAAACTCTATATTAAAAAATGAAAATTC
AGCAGTATCACCATCGATAACAACAACAGATGCATCAGCATCATTAAGTTTTGGGGGTAA
AACAATTGTTTTACCATCAAATCAACCAACAACATTAAGAAAGAGTGATGCACCAGCTGA
TACAAAACATATTAAATTGGAAATTACCAATGATAATAAAATTAATATTATTTGGCAGGA
TGCAAAAAATTATCAATCAAAAGATTGGGTTGCACTTTATAACTATNNNNNNNNNN
Length of 5' end seq. 596
3' end seq. ID VHF449Z
3' end seq. 
>VHF449Z.Seq
NNNNNNNNNNGAATCNATTTTAAAATTTTTAAAATTTAGAATTAATGNACCNAAAAGAAA
AAGTTTTCCANGTTTGNCAAGCNANTTTTACNANTGNANTTTTTTANGAANTTTTTGGAC
ANGANTTAANAACTTGGNCTGTANAACATTGTAANAAANTCAANGAANTTTTTAAAGATN
TTGAAACCTTTGNTT
Length of 3' end seq. 195
Connected seq. ID VHF449P
Connected seq. 
>VHF449P.Seq
TTGGCCTACTGGAAAATTAAATAATTTTTTTTTATATATAATGGAACAAAAAGAATTTGA
TATTAAAAATATTTTATTGAAGATTGAAAAAGATAAAAATGAAACTTCAAAAAAAATACA
AGAAAAATATAAAGATTGTACAATTGAAGATTTGGAAAAATCTTTAAGAATCCAAAATAA
AAAATCAGAAGAAATTACTTTTATCTCAAAAAAGATTGAAGAATTAAATGAAAAATTAAT
AGTTGAAAAATATAAACCATTATTGTCATCGTCCAATAATTCAAATGAAATTGAAAATGA
GATATCACCAAAAATTAAAGAAATTTTAATTAAAAACTCTATATTAAAAAATGAAAATTC
AGCAGTATCACCATCGATAACAACAACAGATGCATCAGCATCATTAAGTTTTGGGGGTAA
AACAATTGTTTTACCATCAAATCAACCAACAACATTAAGAAAGAGTGATGCACCAGCTGA
TACAAAACATATTAAATTGGAAATTACCAATGATAATAAAATTAATATTATTTGGCAGGA
TGCAAAAAATTATCAATCAAAAGATTGGGTTGCACTTTATAACTAT----------GAAT
CNATTTTAAAATTTTTAAAATTTAGAATTAATGNACCNAAAAGAAAAAGTTTTCCANGTT
TGNCAAGCNANTTTTACNANTGNANTTTTTTANGAANTTTTTGGACANGANTTAANAACT
TGGNCTGTANAACATTGTAANAAANTCAANGAANTTTTTAAAGATNTTGAAACCTTTGNT
T
Length of connected seq. 771
Full length Seq ID -
Full length Seq. -
Length of full length seq. -