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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHF441
Library VH
(Link to library)
Clone ID VHF441
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VHF441P
(Link to Original site)
Representative DNA sequence 
>VHF441 (VHF441Q) /CSM/VH/VHF4-B/VHF441Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGXXXXXXXXXXAAGGTGTTCAATATTTGAATGAAANTAA
AGANTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAA
CATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCNTCCACACTGATGCCATCCACAG
AGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTAATCATATGCTGATGAATTAACTG
CCTCCCCAAATTTTTTAGAACCAATTTACTTAGTAGAAATTACTGCCCCAGAAAACGCTA
NTGGTGGTATTTAATCTGTANTCAATCGTCGTAGAGGTATTGTTANTGGTGAAGAACGTC
GTATCGGTTCCCCATTATTATCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTT
TCACCGATGATTTACGTTCCCACACTGCCGGTCAAGNTTTCCCACAATGTGTNTTCGATC
ANTGGGNTTCAANTGGTGTTGTCAACAAAGATAAGAAAGCCNNTGAAGTCGCTTTAGCCN
CCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCNCGAAAAAC
TCTAAACAATTTAAGTCGCACTTTGAGTTTTCAA
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSF---

---GVQYLNEXKXSFVGAFQWATKEGVVCDENMRGIRFNLYDVTXHTDAIHRGGGQIIPT
ARRVIIC**inclpkffrtnllsrnycprkrxwwylicxqss*rycxw*rtsyrfpiiic
qssftsirilrfhr*ftfphcrsxfptmcxrsxgfxwccqqr*esx*srfsxqkekrfsp
rnprs*qiprktlnnlsrtlsfq
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kklisls---

---kvfni*mkxkxlslvlsngppkkvlfvmkt*evsvsifmmspstlmpstevvvkssq
llvv*syadeltaspnflepiylveitapenaxggi*svxnrrrgivxgeerrigsplls
vkahlpvlesfgftddlrshtagqxfpqcvfdxwxsxgvvnkdkkaxevalaxrkrkgla
peipdldkfxekl*ti*val*vf

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rslslfp---

---rcsife*x*rxfrwcfpmghqrrccl**kherypfqsl*chxph*chpqrwwsnhpn
csscnhmlmn*lppqif*nqft**kllpqktlxvvfnlxsivvevllxvknvvsvphyyl
skliyqy*npsvspmiyvptlpvkxshnvxsixgxqxvlstkirkpxksl*pperekv*p
qksqiltnsxknskqfkshfefs

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSF---

---GVQYLNEXKXSFVGAFQWATKEGVVCDENMRGIRFNLYDVTXHTDAIHRGGGQIIPT
ARRVIIC**inclpkffrtnllsrnycprkrxwwylicxqss*rycxw*rtsyrfpiiic
qssftsirilrfhr*ftfphcrsxfptmcxrsxgfxwccqqr*esx*srfsxqkekrfsp
rnprs*qiprktlnnlsrtlsfq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF441 (VHF441Q) /CSM/VH/VHF4-B/VHF441Q.Seq.d/                       2139   0.0
VHF738 (VHF738Q) /CSM/VH/VHF7-B/VHF738Q.Seq.d/                       1088   0.0
VHF716 (VHF716Q) /CSM/VH/VHF7-A/VHF716Q.Seq.d/                       1082   0.0
VHF122 (VHF122Q) /CSM/VH/VHF1-A/VHF122Q.Seq.d/                       1080   0.0
VHP874 (VHP874Q) /CSM/modify/h12/F/treated/VHP874Q.Seq.d/            1074   0.0
VHH874 (VHH874Q) /CSM/VH/VHH8-D/VHH874Q.Seq.d/                       1074   0.0
VHG874 (VHG874Q) /CSM/VH/VHG8-D/VHG874Q.Seq.d/                       1074   0.0
VHP874 (VHP874Q) /CSM/modify/h12/F/treated/VHP874Q.Seq.d/            1074   0.0
VHH874 (VHH874Q) /CSM/VH/VHH8-D/VHH874Q.Seq.d/                       1074   0.0
VHG874 (VHG874Q) /CSM/VH/VHG8-D/VHG874Q.Seq.d/                       1074   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ439678) Dictyostelium discoideum cDNA clone:ddv41b12, 3' ...  1104   0.0    1
(BJ439643) Dictyostelium discoideum cDNA clone:ddv41k05, 3' ...  1080   0.0    1
(BJ427897) Dictyostelium discoideum cDNA clone:ddv63p23, 5' ...  1072   0.0    1
(BJ427892) Dictyostelium discoideum cDNA clone:ddv63o22, 5' ...  1072   0.0    1
(BJ427891) Dictyostelium discoideum cDNA clone:ddv63o21, 5' ...  1072   0.0    1
(BJ427889) Dictyostelium discoideum cDNA clone:ddv63o19, 5' ...  1072   0.0    1
(BJ427888) Dictyostelium discoideum cDNA clone:ddv63n23, 5' ...  1072   0.0    1
(BJ427875) Dictyostelium discoideum cDNA clone:ddv63k24, 5' ...  1072   0.0    1
(BJ427874) Dictyostelium discoideum cDNA clone:ddv63k23, 5' ...  1072   0.0    1
(BJ427873) Dictyostelium discoideum cDNA clone:ddv63k22, 5' ...  1072   0.0    1
dna update 2008.10.29
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM270011_29(AM270011|pid:none) Aspergillus niger contig An02c016...   250   8e-65
AF083031_87(AF083031|pid:none) Guillardia theta nucleomorph chro...   246   1e-63
AF248644_1(AF248644|pid:none) Filobasidiella neoformans var. neo...   245   3e-63
AY026074_1(AY026074|pid:none) Monosiga brevicollis elongation fa...   244   4e-63
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   244   6e-63
DQ357217_1(DQ357217|pid:none) Leishmania braziliensis elongation...   243   7e-63
(Q6FYA7) RecName: Full=Elongation factor 2;          Short=EF-2;...   243   7e-63
AM494972_26(AM494972|pid:none) Leishmania braziliensis chromosom...   243   7e-63
AY618550_1(AY618550|pid:none) Trypanosoma cruzi elongation facto...   243   1e-62
CT005272_19(CT005272|pid:none) Leishmania major strain Friedlin,...   243   1e-62
protein update 2009. 7. 4
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 32.0 %: nuclear
	 20.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for VHF441 is cyt
5' end seq. ID VHF441F
5' end seq. 
>VHF441F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGNNNNNNNNNN
Length of 5' end seq. 572
3' end seq. ID VHF441Z
3' end seq. 
>VHF441Z.Seq
NNNNNNNNNNAAGGTGTTCAATATTTGAATGAAANTAAAGANTCTTTCGTTGGTGCTTTC
CAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAAT
CTTTATGATGTCACCNTCCACACTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCA
ACTGCTCGTCGTGTAATCATATGCTGATGAATTAACTGCCTCCCCAAATTTTTTAGAACC
AATTTACTTAGTAGAAATTACTGCCCCAGAAAACGCTANTGGTGGTATTTAATCTGTANT
CAATCGTCGTAGAGGTATTGTTANTGGTGAAGAACGTCGTATCGGTTCCCCATTATTATC
TGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGATGATTTACGTTCCCA
CACTGCCGGTCAAGNTTTCCCACAATGTGTNTTCGATCANTGGGNTTCAANTGGTGTTGT
CAACAAAGATAAGAAAGCCNNTGAAGTCGCTTTAGCCNCCAGAAAGAGAAAAGGTTTAGC
CCCAGAAATCCCAGATCTTGACAAATTCCNCGAAAAACTCTAAACAATTTAAGTCGCACT
TTGAGTTTTCAA
Length of 3' end seq. 612
Connected seq. ID VHF441P
Connected seq. 
>VHF441P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCG----------AAGGTGTTCAATATTTGAATGAAANTAA
AGANTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAA
CATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCNTCCACACTGATGCCATCCACAG
AGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTAATCATATGCTGATGAATTAACTG
CCTCCCCAAATTTTTTAGAACCAATTTACTTAGTAGAAATTACTGCCCCAGAAAACGCTA
NTGGTGGTATTTAATCTGTANTCAATCGTCGTAGAGGTATTGTTANTGGTGAAGAACGTC
GTATCGGTTCCCCATTATTATCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTT
TCACCGATGATTTACGTTCCCACACTGCCGGTCAAGNTTTCCCACAATGTGTNTTCGATC
ANTGGGNTTCAANTGGTGTTGTCAACAAAGATAAGAAAGCCNNTGAAGTCGCTTTAGCCN
CCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCNCGAAAAAC
TCTAAACAATTTAAGTCGCACTTTGAGTTTTCAA
Length of connected seq. 1164
Full length Seq ID -
Full length Seq. -
Length of full length seq. -