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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHF436
Library VH
(Link to library)
Clone ID VHF436
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VHF436P
(Link to Original site)
Representative DNA sequence 
>VHF436 (VHF436Q) /CSM/VH/VHF4-B/VHF436Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATXXXXXXXXXXTAACCACGAATGGGATAAAAATGATGCCATGAACATTTGGTC
TTTCGGTCCAGAAGGTAACGGTGCCAATNTCATCGTCAATGTCACCAAAGGTGTTCAATA
TTTGAATGAAATTAAAGANTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGT
TGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCTTCCACAC
TGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTANTCATATG
CTGATGAATTAACTGCCTCCCCAANTATCTTAGAACCAATCTACTTAGTAGAAATTACTG
CCCCAGAAAACGCTATTGGTGGTATTTANTCTGTACTCAATCGTCGTAGAGGTATTGTTA
TTGGTGAAGAACGTCGTATCGGTTCCCCATTATTATCTGTCAAAGCTCATTTACCAGTAT
TAGAATCNTTCGGTTTCACCGATGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCAC
AATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCNCTG
AAGTCGCTTTAGCCNCCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACA
AATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTGAGTTTTCA
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTED---

---NHEWDKNDAMNIWSFGPEGNGANXIVNVTKGVQYLNEIKXSFVGAFQWATKEGVVCD
ENMRGIRFNLYDVTFHTDAIHRGGGQIIPTARRVXIC**inclpxylrtnllsrnycprk
rywwylxctqss*rycyw*rtsyrfpiiicqssftsirixrfhr*ftfphcrssfptmcl
rslgfnwccqqr*esx*srfsxqkekrfsprnprs*qiprktlnnlsrt*vf
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kklislsvellnpsmsllvtlk---

---*prmg*k*chehlvfrsrr*rcqxhrqchqrcsife*n*rxfrwcfpmghqrrccl*
*kherypfqsl*chlph*chpqrwwsnhpncsscxhmlmn*lppqxs*nqst**kllpqk
tllvvfxlysivvevlllvknvvsvphyylskliyqy*nxsvspmiyvptlpvklshnvs
siiglqlvlstkirkplksl*pperekv*pqksqiltnstknskqfkshlsf

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rslslfp*sy*irqchcw*h*r---

---NHEWDKNDAMNIWSFGPEGNGANXIVNVTKGVQYLNEIKXSFVGAFQWATKEGVVCD
ENMRGIRFNLYDVTFHTDAIHRGGGQIIPTARRVXIC**inclpxylrtnllsrnycprk
rywwylxctqss*rycyw*rtsyrfpiiicqssftsirixrfhr*ftfphcrssfptmcl
rslgfnwccqqr*esx*srfsxqkekrfsprnprs*qiprktlnnlsrt*vf

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTED---

---ttngikmmp*tfglsvqkvtvpxsssmspkvfni*mklkxlslvlsngppkkvlfvm
kt*evsvsifmmspstlmpstevvvkssqllvvxsyadeltaspxilepiylveitapen
aiggixsvlnrrrgivigeerrigspllsvkahlpvlesfgftddlrshtagqafpqcvf
dhwasigvvnkdkkaxevalaxrkrkglapeipdldkfhekl*ti*valefs
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF436 (VHF436Q) /CSM/VH/VHF4-B/VHF436Q.Seq.d/                       2444   0.0
VHF435 (VHF435Q) /CSM/VH/VHF4-B/VHF435Q.Seq.d/                       2403   0.0
VHF772 (VHF772Q) /CSM/VH/VHF7-C/VHF772Q.Seq.d/                       1314   0.0
VHF660 (VHF660Q) /CSM/VH/VHF6-C/VHF660Q.Seq.d/                       1310   0.0
VHE745 (VHE745Q) /CSM/VH/VHE7-B/VHE745Q.Seq.d/                       1304   0.0
VHF545 (VHF545Q) /CSM/VH/VHF5-B/VHF545Q.Seq.d/                       1298   0.0
VHE768 (VHE768Q) /CSM/VH/VHE7-C/VHE768Q.Seq.d/                       1298   0.0
VHF160 (VHF160Q) /CSM/VH/VHF1-C/VHF160Q.Seq.d/                       1296   0.0
VHF369 (VHF369Q) /CSM/VH/VHF3-C/VHF369Q.Seq.d/                       1292   0.0
VHF609 (VHF609Q) /CSM/VH/VHF6-A/VHF609Q.Seq.d/                       1291   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ439709) Dictyostelium discoideum cDNA clone:ddv41h10, 3' ...  1318   0.0    1
(BJ440129) Dictyostelium discoideum cDNA clone:ddv42p17, 3' ...  1314   0.0    1
(BJ440081) Dictyostelium discoideum cDNA clone:ddv42g16, 3' ...  1310   0.0    1
(BJ439413) Dictyostelium discoideum cDNA clone:ddv40j11, 3' ...  1304   0.0    1
(BJ440011) Dictyostelium discoideum cDNA clone:ddv42i11, 3' ...  1298   0.0    1
(BJ439479) Dictyostelium discoideum cDNA clone:ddv40h17, 3' ...  1298   0.0    1
(BJ439441) Dictyostelium discoideum cDNA clone:ddv40p12, 3' ...  1289   0.0    1
(BJ439943) Dictyostelium discoideum cDNA clone:ddv42k05, 3' ...  1285   0.0    1
(BJ439963) Dictyostelium discoideum cDNA clone:ddv42o04, 3' ...  1283   0.0    1
(BJ439794) Dictyostelium discoideum cDNA clone:ddv41j17, 3' ...  1283   0.0    1
dna update 2008.10.29
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM270011_29(AM270011|pid:none) Aspergillus niger contig An02c016...   264   6e-69
AF248644_1(AF248644|pid:none) Filobasidiella neoformans var. neo...   259   1e-67
(Q6FYA7) RecName: Full=Elongation factor 2;          Short=EF-2;...   258   3e-67
AF083031_87(AF083031|pid:none) Guillardia theta nucleomorph chro...   258   4e-67
AY026074_1(AY026074|pid:none) Monosiga brevicollis elongation fa...   257   7e-67
(Q874B9) RecName: Full=Elongation factor 2;          Short=EF-2;...   257   7e-67
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   256   2e-66
DQ357217_1(DQ357217|pid:none) Leishmania braziliensis elongation...   254   4e-66
AM494972_26(AM494972|pid:none) Leishmania braziliensis chromosom...   254   4e-66
CT005272_19(CT005272|pid:none) Leishmania major strain Friedlin,...   254   5e-66
protein update 2009. 7. 4
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 32.0 %: nuclear
	 20.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for VHF436 is cyt
5' end seq. ID VHF436F
5' end seq. 
>VHF436F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATNNNNNNNNNN
Length of 5' end seq. 618
3' end seq. ID VHF436Z
3' end seq. 
>VHF436Z.Seq
NNNNNNNNNNTAACCACGAATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTC
CAGAAGGTAACGGTGCCAATNTCATCGTCAATGTCACCAAAGGTGTTCAATATTTGAATG
AAATTAAAGANTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTG
ATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCTTCCACACTGATGCCA
TCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTANTCATATGCTGATGAA
TTAACTGCCTCCCCAANTATCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAA
AACGCTATTGGTGGTATTTANTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAA
GAACGTCGTATCGGTTCCCCATTATTATCTGTCAAAGCTCATTTACCAGTATTAGAATCN
TTCGGTTTCACCGATGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTC
TTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCNCTGAAGTCGCT
TTAGCCNCCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCAC
GAAAAACTCTAAACAATTTAAGTCGCACTTGAGTTTTCA
Length of 3' end seq. 699
Connected seq. ID VHF436P
Connected seq. 
>VHF436P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGAT----------TAACCACGAATGGGATAAAAATGATGCCATGAACATTTGGTC
TTTCGGTCCAGAAGGTAACGGTGCCAATNTCATCGTCAATGTCACCAAAGGTGTTCAATA
TTTGAATGAAATTAAAGANTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGT
TGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCTTCCACAC
TGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTANTCATATG
CTGATGAATTAACTGCCTCCCCAANTATCTTAGAACCAATCTACTTAGTAGAAATTACTG
CCCCAGAAAACGCTATTGGTGGTATTTANTCTGTACTCAATCGTCGTAGAGGTATTGTTA
TTGGTGAAGAACGTCGTATCGGTTCCCCATTATTATCTGTCAAAGCTCATTTACCAGTAT
TAGAATCNTTCGGTTTCACCGATGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCAC
AATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCNCTG
AAGTCGCTTTAGCCNCCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACA
AATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTGAGTTTTCA
Length of connected seq. 1297
Full length Seq ID -
Full length Seq. -
Length of full length seq. -