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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHF435
Library VH
(Link to library)
Clone ID VHF435
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VHF435P
(Link to Original site)
Representative DNA sequence 
>VHF435 (VHF435Q) /CSM/VH/VHF4-B/VHF435Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGXXXXXXXXXXGATAACCACGAATGGGATAAAAATGATGCCATGAACATTTGGTC
TTTCGGTCCAGAAGGTAACGGTGCCAATNTCATCGTCAATGTCACCAAAGGTGTTCAATA
TTTGAATGAAATTAAAGANTCTTTCGTTGGTGCTTTCCAAAGGGCCACCAAAGAAGGTGT
TGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCNTCCACAC
TGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTANTATATGC
TGATGAATTAACTGCCTCCCCAANTATCTTAGAACCAATCTACTTAGTAGAAATTACTGC
CCCAGAAAACGCTATTGGTGGTATTTANTCTGTACTCAATCGTCGTAGAGGTATTGTTAT
TGGTGAAGAACGTCGTATCGGTTCCCCATTATTATCTGTCAAAGCTCATTTACCAGTATT
AGAATCNTTCGGTTTCACCGATGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACA
ATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGA
AGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAA
ATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTGAGTTTTCAAAG
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTE---

---DNHEWDKNDAMNIWSFGPEGNGANXIVNVTKGVQYLNEIKXSFVGAFQRATKEGVVC
DENMRGIRFNLYDVTXHTDAIHRGGGQIIPTARRVXYADELTASPXILEPIYLVEITAPE
NAIGGIXSVLNRRRGIVIGEERRIGSPLLSVKAHLPVLESFGFTDDLRSHTAGQAFPQCV
FDHWASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*ti*valefsk
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kklislsvellnpsmsllvtlk---

---DNHEWDKNDAMNIWSFGPEGNGANXIVNVTKGVQYLNEIKXSFVGAFQRATKEGVVC
DENMRGIRFNLYDVTXHTDAIHRGGGQIIPTARRVXYADELTASPXILEPIYLVEITAPE
NAIGGIXSVLNRRRGIVIGEERRIGSPLLSVKAHLPVLESFGFTDDLRSHTAGQAFPQCV
FDHWASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*ti*valefsk

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rslslfp*sy*irqchcw*h*---

---ittngikmmp*tfglsvqkvtvpxsssmspkvfni*mklkxlslvlskgppkkvlfv
mkt*evsvsifmmspstlmpstevvvkssqllvvxymlmn*lppqxs*nqst**kllpqk
tllvvfxlysivvevlllvknvvsvphyylskliyqy*nxsvspmiyvptlpvklshnvs
siiglqlvlstkirkplksl*pperekv*pqksqiltnstknskqfkshlsfq

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTE---

---*prmg*k*chehlvfrsrr*rcqxhrqchqrcsife*n*rxfrwcfpkghqrrccl*
*kherypfqsl*chxph*chpqrwwsnhpncsscxic**inclpxylrtnllsrnycprk
rywwylxctqss*rycyw*rtsyrfpiiicqssftsirixrfhr*ftfphcrssfptmcl
rslgfnwccqqr*esh*srfshqkekrfsprnprs*qiprktlnnlsrt*vfk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF435 (VHF435Q) /CSM/VH/VHF4-B/VHF435Q.Seq.d/                       2454   0.0
VHF436 (VHF436Q) /CSM/VH/VHF4-B/VHF436Q.Seq.d/                       2403   0.0
VHE713 (VHE713Q) /CSM/VH/VHE7-A/VHE713Q.Seq.d/                       1318   0.0
VHF160 (VHF160Q) /CSM/VH/VHF1-C/VHF160Q.Seq.d/                       1312   0.0
VHF660 (VHF660Q) /CSM/VH/VHF6-C/VHF660Q.Seq.d/                       1306   0.0
VHF609 (VHF609Q) /CSM/VH/VHF6-A/VHF609Q.Seq.d/                       1306   0.0
VHF758 (VHF758Q) /CSM/VH/VHF7-C/VHF758Q.Seq.d/                       1304   0.0
VHF538 (VHF538Q) /CSM/VH/VHF5-B/VHF538Q.Seq.d/                       1304   0.0
VHF144 (VHF144Q) /CSM/VH/VHF1-B/VHF144Q.Seq.d/                       1304   0.0
VHF772 (VHF772Q) /CSM/VH/VHF7-C/VHF772Q.Seq.d/                       1302   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ439697) Dictyostelium discoideum cDNA clone:ddv41f10, 3' ...  1330   0.0    1
(BJ439334) Dictyostelium discoideum cDNA clone:ddv40j03, 3' ...  1316   0.0    1
(BJ439779) Dictyostelium discoideum cDNA clone:ddv41g15, 3' ...  1312   0.0    1
(BJ440065) Dictyostelium discoideum cDNA clone:ddv42d15, 3' ...  1304   0.0    1
(BJ439893) Dictyostelium discoideum cDNA clone:ddv42a04, 3' ...  1304   0.0    1
(BJ440019) Dictyostelium discoideum cDNA clone:ddv42k09, 3' ...  1302   0.0    1
(BJ439704) Dictyostelium discoideum cDNA clone:ddv41g11, 3' ...  1302   0.0    1
(BJ440133) Dictyostelium discoideum cDNA clone:ddv42a22, 3' ...  1298   0.0    1
(BJ440053) Dictyostelium discoideum cDNA clone:ddv42b15, 3' ...  1298   0.0    1
(BJ440045) Dictyostelium discoideum cDNA clone:ddv42p09, 3' ...  1298   0.0    1
dna update 2008.10.29
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   265   3e-69
AM270011_29(AM270011|pid:none) Aspergillus niger contig An02c016...   263   8e-69
BC077595_1(BC077595|pid:none) Xenopus laevis translation elongat...   262   2e-68
BT071866_1(BT071866|pid:none) Salmo salar clone ssal-rgf-501-088...   261   3e-68
AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   261   3e-68
BT059038_1(BT059038|pid:none) Salmo salar clone ssal-rgf-521-328...   261   3e-68
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   261   4e-68
BT045031_1(BT045031|pid:none) Salmo salar clone ssal-rgf-510-120...   261   5e-68
BT072304_1(BT072304|pid:none) Salmo salar clone ssal-rgf-520-280...   260   7e-68
BC060707_1(BC060707|pid:none) Mus musculus eukaryotic translatio...   259   2e-67
protein update 2009. 7. 4
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHF435 is cyt
5' end seq. ID VHF435F
5' end seq. 
>VHF435F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGNNNNNNNNNN
Length of 5' end seq. 616
3' end seq. ID VHF435Z
3' end seq. 
>VHF435Z.Seq
NNNNNNNNNNGATAACCACGAATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGG
TCCAGAAGGTAACGGTGCCAATNTCATCGTCAATGTCACCAAAGGTGTTCAATATTTGAA
TGAAATTAAAGANTCTTTCGTTGGTGCTTTCCAAAGGGCCACCAAAGAAGGTGTTGTTTG
TGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCNTCCACACTGATGC
CATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTANTATATGCTGATGA
ATTAACTGCCTCCCCAANTATCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGA
AAACGCTATTGGTGGTATTTANTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGA
AGAACGTCGTATCGGTTCCCCATTATTATCTGTCAAAGCTCATTTACCAGTATTAGAATC
NTTCGGTTTCACCGATGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGT
CTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGC
TTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCA
CGAAAAACTCTAAACAATTTAAGTCGCACTTGAGTTTTCAAAG
Length of 3' end seq. 703
Connected seq. ID VHF435P
Connected seq. 
>VHF435P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAG----------GATAACCACGAATGGGATAAAAATGATGCCATGAACATTTGGTC
TTTCGGTCCAGAAGGTAACGGTGCCAATNTCATCGTCAATGTCACCAAAGGTGTTCAATA
TTTGAATGAAATTAAAGANTCTTTCGTTGGTGCTTTCCAAAGGGCCACCAAAGAAGGTGT
TGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCNTCCACAC
TGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTANTATATGC
TGATGAATTAACTGCCTCCCCAANTATCTTAGAACCAATCTACTTAGTAGAAATTACTGC
CCCAGAAAACGCTATTGGTGGTATTTANTCTGTACTCAATCGTCGTAGAGGTATTGTTAT
TGGTGAAGAACGTCGTATCGGTTCCCCATTATTATCTGTCAAAGCTCATTTACCAGTATT
AGAATCNTTCGGTTTCACCGATGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACA
ATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGA
AGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAA
ATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTGAGTTTTCAAAG
Length of connected seq. 1299
Full length Seq ID -
Full length Seq. -
Length of full length seq. -