NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHF434
Library VH
(Link to library)
Clone ID VHF434
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16429-1
Original site URL
Representative seq. ID VHF434P
(Link to Original site)
Representative DNA sequence 
>VHF434 (VHF434Q) /CSM/VH/VHF4-B/VHF434Q.Seq.d/
TGGCCTACTGGAATTTATATAAATTACCTTATTACTTAAAGATGACAACAAATAATCCAT
TTGATAAAGTAAAGGATGTATTAAAATCACAAGATCAAACTTATAATTTTTATAATCTTT
CAAAACTTCAAGACCCAAGAATTGAAAAATTACCATACTCCATTAGAATTCTTTTGGAAT
CAGCAGTTCGTAATTGTGATAACTTTGAAGTACACGAAAAAGATGTTGAAAATATCTTAA
ATTGGGAAAACACTGCAAATAAAGTTGAAATTCCTTTCAAACCAGCAAGAGTATTATTAC
AAGATTTTACTGGTGTACCAGCAGTTGTTGATCTTGCAGCAATGAGAGATGCCATGAAAA
GATTAGGTGGTGATCCAGCAAAAATTAACCCACTTGTTCCAGTTGATTTAGTTATTGATC
ACTCTGTTCAAGTTGATGTATCAAGAACTGTTGATGCTTTAGAGCAAAATCAAAAGATTG
AATTCCATAGAAATCATGAAAGATTTTCATTTTTAAAATGGGGTGCTCAAGCATTTGATG
GATTATTTATTGCTCXXXXXXXXXXAATCTTGGTGATAGTATTACCACTGATCATATTTC
ACCAGCCGGTAACATCNATAGAAAATCATCAGCCGCTCGTTATTTAGAGTCCAAAGGTGT
AAAACCAGAAGATTTTAACACTTATGGATCTCGTAGAGGTAATGATGAAATTATGGTTAG
AGGTACNTTTGCCAATACTCGTATTGTTAATAAATTGGCTCCAGCCGTTGGTCCACAAAC
AACTTATGTACCAACTGGTGAGTTAATGTTTATTTCAGATGCTGCAGAAAAATATCAATC
AGAAGGTCATCAACTCATTGTTTTGGCTGGTAGTGATTATGGNTCTGGTAGTTNTAGAGA
TTGGGCTGCCAAAGGTCCATACNTTCAAGGAATTAAGTGTGTCATTGCCATCTCATTTGA
ACGTATTCATAGATCCAATCTCGTTGGTATGGGTATTATCCCATTGCAATTCCAACCAGG
TCAAAATGCNTCTACTTTGGGTCTCACTGGTAAAGAACAATTCAATATCGAGTTACCAAC
TGATAAATCCNTCATTAAAACTGGTCAAACNGTAAAGGTTACCNCTAATTGTGGTAAATC
TTTTGAAACTATCNTTCGTTTCGATACTCCNATCGAAGTTGAATATTGGGCAAATAATGG
TATCTTATCTTATGTTTTAAGAAAACTTTTACATTAATTTCTTTTTTTAATATAAAATAA
AAAAAAATAAT
sequence update 2002.10.25
Translated Amino Acid sequence 
AYWNLYKLPYYLKMTTNNPFDKVKDVLKSQDQTYNFYNLSKLQDPRIEKLPYSIRILLES
AVRNCDNFEVHEKDVENILNWENTANKVEIPFKPARVLLQDFTGVPAVVDLAAMRDAMKR
LGGDPAKINPLVPVDLVIDHSVQVDVSRTVDALEQNQKIEFHRNHERFSFLKWGAQAFDG
LFIA---

---NLGDSITTDHISPAGNIXRKSSAARYLESKGVKPEDFNTYGSRRGNDEIMVRGTFAN
TRIVNKLAPAVGPQTTYVPTGELMFISDAAEKYQSEGHQLIVLAGSDYGSGSXRDWAAKG
PYXQGIKCVIAISFERIHRSNLVGMGIIPLQFQPGQNASTLGLTGKEQFNIELPTDKSXI
KTGQTVKVTXNCGKSFETIXRFDTPIEVEYWANNGILSYVLRKLLH*flfli*nkkk*
Translated Amino Acid sequence (All Frames) 
Frame A:
wptgiyinylit*r*qqiihlik*rmy*nhkikliifiifqnfktqelknyhtpleffwn
qqfvivitlkytkkmlkis*igktlqiklkflsnqqeyyykillvyqqllilqq*emp*k
d*vviqqklthlfqli*llitlfklmyqellml*skikrlnsieimkdfhf*ngvlkhlm
dylll---

---NLGDSITTDHISPAGNIXRKSSAARYLESKGVKPEDFNTYGSRRGNDEIMVRGTFAN
TRIVNKLAPAVGPQTTYVPTGELMFISDAAEKYQSEGHQLIVLAGSDYGSGSXRDWAAKG
PYXQGIKCVIAISFERIHRSNLVGMGIIPLQFQPGQNASTLGLTGKEQFNIELPTDKSXI
KTGQTVKVTXNCGKSFETIXRFDTPIEVEYWANNGILSYVLRKLLH*flfli*nkkk*

Frame B:
gllefi*itlllkddnk*si**skgcikitrsnl*fl*sfktsrpkn*kitilh*nsfgi
sss*l**l*strkrc*kylklgkhck*s*nsfqtsksiitrfywctssc*scsnerchek
irw*sskn*ptcss*fsy*slcss*ciknc*cfrakskd*ip*ks*kififkmgcssi*w
iiyc---

---ilvivlpliifhqpvtsienhqplvi*spkv*nqkiltlmdlvevmmklwlevxlpi
lvllinwlqplvhkqlmyqlvs*clfqmlqkninqkvinslfwlvvimxlvvxeiglpkv
htfkelsvslpshlnvfidpislvwvlshcnsnqvkmxllwvslvknnsissyqlinpsl
klvkx*rlplivvnllklsfvsilxsklnigqimvsylmf*enfyinfff*ykikknn

Frame C:
AYWNLYKLPYYLKMTTNNPFDKVKDVLKSQDQTYNFYNLSKLQDPRIEKLPYSIRILLES
AVRNCDNFEVHEKDVENILNWENTANKVEIPFKPARVLLQDFTGVPAVVDLAAMRDAMKR
LGGDPAKINPLVPVDLVIDHSVQVDVSRTVDALEQNQKIEFHRNHERFSFLKWGAQAFDG
LFIA---

---sw**yyh*syftsr*hx*kiisrslfrvqrcktrrf*hlwis*r***nyg*ryxcqy
syc**igssrwstnnlctnw*vnvyfrccrkisirrssthcfgw**lwxw*x*rlgcqrs
ixsrn*vchchli*tys*iqsrwygyypiaiptrskcxyfgshw*rtiqyrvtn**ixh*
nwsnxkgyx*lw*if*nyxsfrysxrs*ilgk*wylilcfkktftlisffnik*kki
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF434 (VHF434Q) /CSM/VH/VHF4-B/VHF434Q.Seq.d/                       2335   0.0
VHF558 (VHF558Q) /CSM/VH/VHF5-C/VHF558Q.Seq.d/                       1265   0.0
CHH576 (CHH576Q) /CSM/CH/CHH5-D/CHH576Q.Seq.d/                       1263   0.0
CHH556 (CHH556Q) /CSM/CH/CHH5-C/CHH556Q.Seq.d/                       1263   0.0
SHF379 (SHF379Q) /CSM/SH/SHF3-D/SHF379Q.Seq.d/                       1261   0.0
CHQ274 (CHQ274Q) /CSM/CH/CHQ2-D/CHQ274Q.Seq.d/                       1261   0.0
VHQ461 (VHQ461Q) /CSM/VH/VHQ4-C/VHQ461Q.Seq.d/                       1259   0.0
VHQ432 (VHQ432Q) /CSM/VH/VHQ4-B/VHQ432Q.Seq.d/                       1259   0.0
VHO844 (VHO844Q) /CSM/VH/VHO8-B/VHO844Q.Seq.d/                       1259   0.0
VHA714 (VHA714Q) /CSM/VH/VHA7-A/VHA714Q.Seq.d/                       1259   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ439687) Dictyostelium discoideum cDNA clone:ddv41d10, 3' ...  1275   0.0    1
(BJ440059) Dictyostelium discoideum cDNA clone:ddv42c15, 3' ...  1265   0.0    1
(BJ380332) Dictyostelium discoideum cDNA clone:ddc37g19, 3' ...  1263   0.0    1
(BJ380294) Dictyostelium discoideum cDNA clone:ddc37o13, 3' ...  1263   0.0    1
(BJ406337) Dictyostelium discoideum cDNA clone:dds34n19, 3' ...  1261   0.0    1
(BJ385380) Dictyostelium discoideum cDNA clone:ddc54c20, 3' ...  1261   0.0    1
(BJ446718) Dictyostelium discoideum cDNA clone:ddv63j16, 3' ...  1259   0.0    1
(BJ446667) Dictyostelium discoideum cDNA clone:ddv63p08, 3' ...  1259   0.0    1
(BJ445829) Dictyostelium discoideum cDNA clone:ddv60d20, 3' ...  1259   0.0    1
(BJ436760) Dictyostelium discoideum cDNA clone:ddv32l03, 3' ...  1259   0.0    1
dna update 2008.10.29
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54X73) RecName: Full=Probable cytoplasmic aconitate hydratase;...   436   e-121
(Q23500) RecName: Full=Probable cytoplasmic aconitate hydratase;...   288   2e-76
AB236788_1(AB236788|pid:none) Trifolium pratense RNA for putativ...   288   3e-76
AP008209_256(AP008209|pid:none) Oryza sativa (japonica cultivar-...   286   8e-76
BT060820_1(BT060820|pid:none) Zea mays full-length cDNA clone ZM...   285   3e-75
BT060665_1(BT060665|pid:none) Zea mays full-length cDNA clone ZM...   284   5e-75
DQ294230_1(DQ294230|pid:none) Glossina morsitans morsitans TC165...   283   1e-74
BC168529_1(BC168529|pid:none) Xenopus tropicalis cDNA clone MGC:...   281   3e-74
(P49608) RecName: Full=Aconitate hydratase, cytoplasmic;        ...   281   3e-74
(Q9SIB9) RecName: Full=Aconitate hydratase 2, mitochondrial;    ...   281   4e-74
protein update 2009. 7. 4
PSORT 

 psg: 0.75  gvh: 0.20  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.29  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 36.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: plasma membrane
	  4.0 %: extracellular, including cell wall

>> prediction for VHF434 is nuc
5' end seq. ID VHF434F
5' end seq. 
>VHF434F.Seq
TGGCCTACTGGAATTTATATAAATTACCTTATTACTTAAAGATGACAACAAATAATCCAT
TTGATAAAGTAAAGGATGTATTAAAATCACAAGATCAAACTTATAATTTTTATAATCTTT
CAAAACTTCAAGACCCAAGAATTGAAAAATTACCATACTCCATTAGAATTCTTTTGGAAT
CAGCAGTTCGTAATTGTGATAACTTTGAAGTACACGAAAAAGATGTTGAAAATATCTTAA
ATTGGGAAAACACTGCAAATAAAGTTGAAATTCCTTTCAAACCAGCAAGAGTATTATTAC
AAGATTTTACTGGTGTACCAGCAGTTGTTGATCTTGCAGCAATGAGAGATGCCATGAAAA
GATTAGGTGGTGATCCAGCAAAAATTAACCCACTTGTTCCAGTTGATTTAGTTATTGATC
ACTCTGTTCAAGTTGATGTATCAAGAACTGTTGATGCTTTAGAGCAAAATCAAAAGATTG
AATTCCATAGAAATCATGAAAGATTTTCATTTTTAAAATGGGGTGCTCAAGCATTTGATG
GATTATTTATTGCTCNNNNNNNNNN
Length of 5' end seq. 565
3' end seq. ID VHF434Z
3' end seq. 
>VHF434Z.Seq
NNNNNNNNNNAATCTTGGTGATAGTATTACCACTGATCATATTTCACCAGCCGGTAACAT
CNATAGAAAATCATCAGCCGCTCGTTATTTAGAGTCCAAAGGTGTAAAACCAGAAGATTT
TAACACTTATGGATCTCGTAGAGGTAATGATGAAATTATGGTTAGAGGTACNTTTGCCAA
TACTCGTATTGTTAATAAATTGGCTCCAGCCGTTGGTCCACAAACAACTTATGTACCAAC
TGGTGAGTTAATGTTTATTTCAGATGCTGCAGAAAAATATCAATCAGAAGGTCATCAACT
CATTGTTTTGGCTGGTAGTGATTATGGNTCTGGTAGTTNTAGAGATTGGGCTGCCAAAGG
TCCATACNTTCAAGGAATTAAGTGTGTCATTGCCATCTCATTTGAACGTATTCATAGATC
CAATCTCGTTGGTATGGGTATTATCCCATTGCAATTCCAACCAGGTCAAAATGCNTCTAC
TTTGGGTCTCACTGGTAAAGAACAATTCAATATCGAGTTACCAACTGATAAATCCNTCAT
TAAAACTGGTCAAACNGTAAAGGTTACCNCTAATTGTGGTAAATCTTTTGAAACTATCNT
TCGTTTCGATACTCCNATCGAAGTTGAATATTGGGCAAATAATGGTATCTTATCTTATGT
TTTAAGAAAACTTTTACATTAATTTCTTTTTTTAATATAAAATAAAAAAAAATAAT
Length of 3' end seq. 716
Connected seq. ID VHF434P
Connected seq. 
>VHF434P.Seq
TGGCCTACTGGAATTTATATAAATTACCTTATTACTTAAAGATGACAACAAATAATCCAT
TTGATAAAGTAAAGGATGTATTAAAATCACAAGATCAAACTTATAATTTTTATAATCTTT
CAAAACTTCAAGACCCAAGAATTGAAAAATTACCATACTCCATTAGAATTCTTTTGGAAT
CAGCAGTTCGTAATTGTGATAACTTTGAAGTACACGAAAAAGATGTTGAAAATATCTTAA
ATTGGGAAAACACTGCAAATAAAGTTGAAATTCCTTTCAAACCAGCAAGAGTATTATTAC
AAGATTTTACTGGTGTACCAGCAGTTGTTGATCTTGCAGCAATGAGAGATGCCATGAAAA
GATTAGGTGGTGATCCAGCAAAAATTAACCCACTTGTTCCAGTTGATTTAGTTATTGATC
ACTCTGTTCAAGTTGATGTATCAAGAACTGTTGATGCTTTAGAGCAAAATCAAAAGATTG
AATTCCATAGAAATCATGAAAGATTTTCATTTTTAAAATGGGGTGCTCAAGCATTTGATG
GATTATTTATTGCTC----------AATCTTGGTGATAGTATTACCACTGATCATATTTC
ACCAGCCGGTAACATCNATAGAAAATCATCAGCCGCTCGTTATTTAGAGTCCAAAGGTGT
AAAACCAGAAGATTTTAACACTTATGGATCTCGTAGAGGTAATGATGAAATTATGGTTAG
AGGTACNTTTGCCAATACTCGTATTGTTAATAAATTGGCTCCAGCCGTTGGTCCACAAAC
AACTTATGTACCAACTGGTGAGTTAATGTTTATTTCAGATGCTGCAGAAAAATATCAATC
AGAAGGTCATCAACTCATTGTTTTGGCTGGTAGTGATTATGGNTCTGGTAGTTNTAGAGA
TTGGGCTGCCAAAGGTCCATACNTTCAAGGAATTAAGTGTGTCATTGCCATCTCATTTGA
ACGTATTCATAGATCCAATCTCGTTGGTATGGGTATTATCCCATTGCAATTCCAACCAGG
TCAAAATGCNTCTACTTTGGGTCTCACTGGTAAAGAACAATTCAATATCGAGTTACCAAC
TGATAAATCCNTCATTAAAACTGGTCAAACNGTAAAGGTTACCNCTAATTGTGGTAAATC
TTTTGAAACTATCNTTCGTTTCGATACTCCNATCGAAGTTGAATATTGGGCAAATAATGG
TATCTTATCTTATGTTTTAAGAAAACTTTTACATTAATTTCTTTTTTTAATATAAAATAA
AAAAAAATAAT
Length of connected seq. 1261
Full length Seq ID -
Full length Seq. -
Length of full length seq. -