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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHF411
Library VH
(Link to library)
Clone ID VHF411
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VHF411P
(Link to Original site)
Representative DNA sequence 
>VHF411 (VHF411Q) /CSM/VH/VHF4-A/VHF411Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATXXXXXXXXXXGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGA
AGGTAACGGTGCCAATNTCTTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAAT
TAAAGANTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGA
AAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCA
CAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTANTCATATGCTGCTGAATTAA
CTGCCCCCCCAANTATCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACG
CTATTGGTGGTATTTAATCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAAC
GTCGTATCGGTTCCCCATTATTATCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCG
GTTTCACCGATGATTTACGTTCCCACACTGCCGGTCAAGNTTTCCCACAATGTGTNTTCG
ATCANTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAG
CCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAA
AACTCTAAACAATTTAAGTCGCACTTGAGTTTTCAAAG
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTED---

---DKNDAMNIWSFGPEGNGANXFVNVTKGVQYLNEIKXSFVGAFQWATKEGVVCDENMR
GIRFNLYDVTLHTDAIHRGGGQIIPTARRVXICC*incppxylrtnllsrnycprkryww
ylictqss*rycyw*rtsyrfpiiicqssftsirilrfhr*ftfphcrsxfptmcxrsxg
fnwccqqr*esh*srfshqkekrfsprnprs*qiprktlnnlsrt*vfk
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kklislsvellnpsmsllvtlk---

---g*k*chehlvfrsrr*rcqxlrqchqrcsife*n*rxfrwcfpmghqrrccl**khe
rypfqsl*chpph*chpqrwwsnhpncsscxhmlln*lppqxs*nqst**kllpqktllv
vfnlysivvevlllvknvvsvphyylskliyqy*npsvspmiyvptlpvkxshnvxsixg
lqlvlstkirkplksl*pperekv*pqksqiltnstknskqfkshlsfq

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rslslfp*sy*irqchcw*h*r---

---DKNDAMNIWSFGPEGNGANXFVNVTKGVQYLNEIKXSFVGAFQWATKEGVVCDENMR
GIRFNLYDVTLHTDAIHRGGGQIIPTARRVXICC*incppxylrtnllsrnycprkryww
ylictqss*rycyw*rtsyrfpiiicqssftsirilrfhr*ftfphcrsxfptmcxrsxg
fnwccqqr*esh*srfshqkekrfsprnprs*qiprktlnnlsrt*vfk

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTED---

---ikmmp*tfglsvqkvtvpxsssmspkvfni*mklkxlslvlsngppkkvlfvmkt*e
vsvsifmmspstlmpstevvvkssqllvvxsyaaeltappxilepiylveitapenaigg
i*svlnrrrgivigeerrigspllsvkahlpvlesfgftddlrshtagqxfpqcvfdxwa
sigvvnkdkkatevalatrkrkglapeipdldkfhekl*ti*valefsk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF411 (VHF411Q) /CSM/VH/VHF4-A/VHF411Q.Seq.d/                       2391   0.0
VHE779 (VHE779Q) /CSM/VH/VHE7-D/VHE779Q.Seq.d/                       1243   0.0
VHF660 (VHF660Q) /CSM/VH/VHF6-C/VHF660Q.Seq.d/                       1237   0.0
VHF112 (VHF112Q) /CSM/VH/VHF1-A/VHF112Q.Seq.d/                       1235   0.0
VHF740 (VHF740Q) /CSM/VH/VHF7-B/VHF740Q.Seq.d/                       1231   0.0
VHF772 (VHF772Q) /CSM/VH/VHF7-C/VHF772Q.Seq.d/                       1229   0.0
VHF679 (VHF679Q) /CSM/VH/VHF6-D/VHF679Q.Seq.d/                       1225   0.0
VHF616 (VHF616Q) /CSM/VH/VHF6-A/VHF616Q.Seq.d/                       1225   0.0
VHF264 (VHF264Q) /CSM/VH/VHF2-C/VHF264Q.Seq.d/                       1223   0.0
VHF545 (VHF545Q) /CSM/VH/VHF5-B/VHF545Q.Seq.d/                       1221   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ439625) Dictyostelium discoideum cDNA clone:ddv41f04, 3' ...  1261   0.0    1
(BJ440081) Dictyostelium discoideum cDNA clone:ddv42g16, 3' ...  1233   0.0    1
(BJ440129) Dictyostelium discoideum cDNA clone:ddv42p17, 3' ...  1225   0.0    1
(BJ439963) Dictyostelium discoideum cDNA clone:ddv42o04, 3' ...  1221   0.0    1
(BJ440011) Dictyostelium discoideum cDNA clone:ddv42i11, 3' ...  1217   0.0    1
(BJ439479) Dictyostelium discoideum cDNA clone:ddv40h17, 3' ...  1213   0.0    1
(BJ439709) Dictyostelium discoideum cDNA clone:ddv41h10, 3' ...  1207   0.0    1
(BJ439794) Dictyostelium discoideum cDNA clone:ddv41j17, 3' ...  1185   0.0    2
(BJ427897) Dictyostelium discoideum cDNA clone:ddv63p23, 5' ...  1166   0.0    1
(BJ427892) Dictyostelium discoideum cDNA clone:ddv63o22, 5' ...  1166   0.0    1
dna update 2008.10.29
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM270011_29(AM270011|pid:none) Aspergillus niger contig An02c016...   264   6e-69
AF248644_1(AF248644|pid:none) Filobasidiella neoformans var. neo...   259   1e-67
(Q6FYA7) RecName: Full=Elongation factor 2;          Short=EF-2;...   258   3e-67
AF083031_87(AF083031|pid:none) Guillardia theta nucleomorph chro...   258   4e-67
AY026074_1(AY026074|pid:none) Monosiga brevicollis elongation fa...   257   7e-67
(Q874B9) RecName: Full=Elongation factor 2;          Short=EF-2;...   257   7e-67
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   256   2e-66
DQ357217_1(DQ357217|pid:none) Leishmania braziliensis elongation...   254   4e-66
AM494972_26(AM494972|pid:none) Leishmania braziliensis chromosom...   254   4e-66
CT005272_19(CT005272|pid:none) Leishmania major strain Friedlin,...   254   5e-66
protein update 2009. 7. 4
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 32.0 %: nuclear
	 20.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for VHF411 is cyt
5' end seq. ID VHF411F
5' end seq. 
>VHF411F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATNNNNNNNNNN
Length of 5' end seq. 618
3' end seq. ID VHF411Z
3' end seq. 
>VHF411Z.Seq
NNNNNNNNNNGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAGGTAACG
GTGCCAATNTCTTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGANT
CTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGA
GAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGGTG
GTGGTCAAATCATCCCAACTGCTCGTCGTGTANTCATATGCTGCTGAATTAACTGCCCCC
CCAANTATCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGT
GGTATTTAATCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATC
GGTTCCCCATTATTATCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACC
GATGATTTACGTTCCCACACTGCCGGTCAAGNTTTCCCACAATGTGTNTTCGATCANTGG
GCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGA
AAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAA
ACAATTTAAGTCGCACTTGAGTTTTCAAAG
Length of 3' end seq. 690
Connected seq. ID VHF411P
Connected seq. 
>VHF411P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGAT----------GGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGA
AGGTAACGGTGCCAATNTCTTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAAT
TAAAGANTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGA
AAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCA
CAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTANTCATATGCTGCTGAATTAA
CTGCCCCCCCAANTATCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACG
CTATTGGTGGTATTTAATCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAAC
GTCGTATCGGTTCCCCATTATTATCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCG
GTTTCACCGATGATTTACGTTCCCACACTGCCGGTCAAGNTTTCCCACAATGTGTNTTCG
ATCANTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAG
CCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAA
AACTCTAAACAATTTAAGTCGCACTTGAGTTTTCAAAG
Length of connected seq. 1288
Full length Seq ID -
Full length Seq. -
Length of full length seq. -