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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHF394
Library VH
(Link to library)
Clone ID VHF394
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VHF394P
(Link to Original site)
Representative DNA sequence 
>VHF394 (VHF394Q) /CSM/VH/VHF3-D/VHF394Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGGGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTXXXXXXXXXXGATGAATTAACTGCNTCCCCAANTATCTTAGAACCAA
TCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTANTCTGTANTCA
ATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTATCTG
TCAAAGCTCATTTACCAGTATTAGAATCNTTCGGTTTCACCGATGATTTACGTTCCCACA
CTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCA
ACAAAGATAAGAAAGCCNCTGAAGTCGCTTTAGCCNCCAGAAAGAGAAAAGGTTTAGCCC
CAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACATN
AGTTTTCAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAY---

---DELTASPXILEPIYLVEITAPENAIGGIXSVXNRRRGIVIGEERRIGSPLLSVKAHL
PVLESFGFTDDLRSHTAGQAFPQCVFDHWASIGVVNKDKKAXEVALAXRKRKGLAPEIPD
LDKFHEKL*ti*vahxfs
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skglvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kkli---

---DELTASPXILEPIYLVEITAPENAIGGIXSVXNRRRGIVIGEERRIGSPLLSVKAHL
PVLESFGFTDDLRSHTAGQAFPQCVFDHWASIGVVNKDKKAXEVALAXRKRKGLAPEIPD
LDKFHEKL*ti*vahxfs

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkgwyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rsls---

---mn*lxpqxs*nqst**kllpqktllvvfxlxsivvevlllvknvvsvphyylskliy
qy*nxsvspmiyvptlpvklshnvssiiglqlvlstkirkplksl*pperekv*pqksqi
ltnstknskqfkshxsfq

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAY---

---*incxpxylrtnllsrnycprkrywwylxcxqss*rycyw*rtsyrfpiiicqssft
sirixrfhr*ftfphcrssfptmclrslgfnwccqqr*esx*srfsxqkekrfsprnprs
*qiprktlnnlsrtxvfk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF394 (VHF394Q) /CSM/VH/VHF3-D/VHF394Q.Seq.d/                       1776   0.0
VHH481 (VHH481Q) /CSM/VH/VHH4-D/VHH481Q.Seq.d/                       1057   0.0
VHE751 (VHE751Q) /CSM/VH/VHE7-C/VHE751Q.Seq.d/                       1057   0.0
VHC537 (VHC537Q) /CSM/VH/VHC5-B/VHC537Q.Seq.d/                       1057   0.0
VHP874 (VHP874Q) /CSM/modify/h12/F/treated/VHP874Q.Seq.d/            1049   0.0
VHH874 (VHH874Q) /CSM/VH/VHH8-D/VHH874Q.Seq.d/                       1049   0.0
VHG874 (VHG874Q) /CSM/VH/VHG8-D/VHG874Q.Seq.d/                       1049   0.0
VHP874 (VHP874Q) /CSM/modify/h12/F/treated/VHP874Q.Seq.d/            1049   0.0
VHH874 (VHH874Q) /CSM/VH/VHH8-D/VHH874Q.Seq.d/                       1049   0.0
VHG874 (VHG874Q) /CSM/VH/VHG8-D/VHG874Q.Seq.d/                       1049   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ422387) Dictyostelium discoideum cDNA clone:ddv45b22, 5' ...  1057   0.0    1
(BJ421159) Dictyostelium discoideum cDNA clone:ddv41l23, 5' ...  1057   0.0    1
(BJ420822) Dictyostelium discoideum cDNA clone:ddv40f13, 5' ...  1057   0.0    1
(BJ419518) Dictyostelium discoideum cDNA clone:ddv36i09, 5' ...  1057   0.0    1
(BJ427897) Dictyostelium discoideum cDNA clone:ddv63p23, 5' ...  1049   0.0    1
(BJ427892) Dictyostelium discoideum cDNA clone:ddv63o22, 5' ...  1049   0.0    1
(BJ427891) Dictyostelium discoideum cDNA clone:ddv63o21, 5' ...  1049   0.0    1
(BJ427889) Dictyostelium discoideum cDNA clone:ddv63o19, 5' ...  1049   0.0    1
(BJ427888) Dictyostelium discoideum cDNA clone:ddv63n23, 5' ...  1049   0.0    1
(BJ427875) Dictyostelium discoideum cDNA clone:ddv63k24, 5' ...  1049   0.0    1
dna update 2008.10.29
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM270011_29(AM270011|pid:none) Aspergillus niger contig An02c016...   246   1e-63
AF083031_87(AF083031|pid:none) Guillardia theta nucleomorph chro...   243   6e-63
DQ357217_1(DQ357217|pid:none) Leishmania braziliensis elongation...   242   2e-62
AM494972_26(AM494972|pid:none) Leishmania braziliensis chromosom...   242   2e-62
AY618550_1(AY618550|pid:none) Trypanosoma cruzi elongation facto...   241   2e-62
CT005272_19(CT005272|pid:none) Leishmania major strain Friedlin,...   241   2e-62
AF248644_1(AF248644|pid:none) Filobasidiella neoformans var. neo...   241   2e-62
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   239   8e-62
(Q6FYA7) RecName: Full=Elongation factor 2;          Short=EF-2;...   239   1e-61
(Q874B9) RecName: Full=Elongation factor 2;          Short=EF-2;...   239   1e-61
protein update 2009. 7. 4
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHF394 is cyt
5' end seq. ID VHF394F
5' end seq. 
>VHF394F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGGGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTNNNNNNNNNN
Length of 5' end seq. 563
3' end seq. ID VHF394Z
3' end seq. 
>VHF394Z.Seq
NNNNNNNNNNGATGAATTAACTGCNTCCCCAANTATCTTAGAACCAATCTACTTAGTAGA
AATTACTGCCCCAGAAAACGCTATTGGTGGTATTTANTCTGTANTCAATCGTCGTAGAGG
TATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTATCTGTCAAAGCTCATTT
ACCAGTATTAGAATCNTTCGGTTTCACCGATGATTTACGTTCCCACACTGCCGGTCAAGC
TTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAA
AGCCNCTGAAGTCGCTTTAGCCNCCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGA
TCTTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACATNAGTTTTCAAA
Length of 3' end seq. 417
Connected seq. ID VHF394P
Connected seq. 
>VHF394P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGGGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCT----------GATGAATTAACTGCNTCCCCAANTATCTTAGAACCAA
TCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTANTCTGTANTCA
ATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTATCTG
TCAAAGCTCATTTACCAGTATTAGAATCNTTCGGTTTCACCGATGATTTACGTTCCCACA
CTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCA
ACAAAGATAAGAAAGCCNCTGAAGTCGCTTTAGCCNCCAGAAAGAGAAAAGGTTTAGCCC
CAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACATN
AGTTTTCAAA
Length of connected seq. 960
Full length Seq ID -
Full length Seq. -
Length of full length seq. -