NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHF375
Library VH
(Link to library)
Clone ID VHF375
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VHF375P
(Link to Original site)
Representative DNA sequence 
>VHF375 (VHF375Q) /CSM/VH/VHF3-D/VHF375Q.Seq.d/
AACTCTCGAGTGCAAAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAAT
TCGTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGA
TCACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAA
AGTTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCAC
CATTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGC
TGGTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTT
CAGTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTG
CGXXXXXXXXXXAGGTAACGGTGCCAATNTCNTCGTCAATGTCACCAAAGGTGTTCAATA
TTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGT
TGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACAC
TGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGC
TGCTGAATTAACTGCCTCCCCAACTNTCTTAGAACCAATCTACTTAGTAGAAATTACTGC
CCCAGAAAACGCTATTGGTGGTATTTANTCTGTACTCAATCGTCGTAGAGGTATTGTTAT
TGGTGAAGAACGTCGTATCGGTTCCCCATTATTATCTGTCAAAGCTCATTTACCAGTATT
AGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACA
ATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGA
AGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAA
ATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTNAGTTTTCAAAGATAGNAATCAA
ATCTAAAAAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
TLECKKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADK
VSGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDF
SSEVTAALRVTDGALVVIDC---

---GNGANXXVNVTKGVQYLNEIKDSFVGAFQWATKEGVVCDENMRGIRFNLYDVTLHTD
AIHRGGGQIIPTARRVLYAAELTASPTXLEPIYLVEITAPENAIGGIXSVLNRRRGIVIG
EERRIGSPLLSVKAHLPVLESFGFTADLRSHTAGQAFPQCVFDHWASIGVVNKDKKATEV
ALATRKRKGLAPEIPDLDKFHEKL*ti*valxfskixikskk
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*
sfw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*f
qfrsnccppcy*wcfsryrl---

---r*rcqxxrqchqrcsife*n*rffrwcfpmghqrrccl**kherypfqsl*chpph*
chpqrwwsnhpncssctlcc*inclpnxlrtnllsrnycprkrywwylxctqss*rycyw
*rtsyrfpiiicqssftsirilrfhr*ftfphcrssfptmclrslgfnwccqqr*esh*s
rfshqkekrfsprnprs*qiprktlnnlsrtxvfkdxnqi*kk

Frame B:
TLECKKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADK
VSGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDF
SSEVTAALRVTDGALVVIDC---

---GNGANXXVNVTKGVQYLNEIKDSFVGAFQWATKEGVVCDENMRGIRFNLYDVTLHTD
AIHRGGGQIIPTARRVLYAAELTASPTXLEPIYLVEITAPENAIGGIXSVLNRRRGIVIG
EERRIGSPLLSVKAHLPVLESFGFTADLRSHTAGQAFPQCVFDHWASIGVVNKDKKATEV
ALATRKRKGLAPEIPDLDKFHEKL*ti*valxfskixikskk

Frame C:
lssakkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsia---

---vtvpxsssmspkvfni*mklkilslvlsngppkkvlfvmkt*evsvsifmmspstlm
pstevvvkssqllvvysmlln*lppqls*nqst**kllpqktllvvfxlysivvevlllv
knvvsvphyylskliyqy*npsvspliyvptlpvklshnvssiiglqlvlstkirkplks
l*pperekv*pqksqiltnstknskqfkshxsfqr*xsnlkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF375 (VHF375Q) /CSM/VH/VHF3-D/VHF375Q.Seq.d/                       2010   0.0
VHQ410 (VHQ410Q) /CSM/VH/VHQ4-A/VHQ410Q.Seq.d/                       1243   0.0
VHQ346 (VHQ346Q) /CSM/VH/VHQ3-B/VHQ346Q.Seq.d/                       1243   0.0
VHQ292 (VHQ292Q) /CSM/VH/VHQ2-D/VHQ292Q.Seq.d/                       1243   0.0
VHQ186 (VHQ186Q) /CSM/VH/VHQ1-D/VHQ186Q.Seq.d/                       1243   0.0
VHP861 (VHP861Q) /CSM/VH/VHP8-C/VHP861Q.Seq.d/                       1243   0.0
VHP860 (VHP860Q) /CSM/VH/VHP8-C/VHP860Q.Seq.d/                       1243   0.0
VHP851 (VHP851Q) /CSM/VH/VHP8-C/VHP851Q.Seq.d/                       1243   0.0
VHP847 (VHP847Q) /CSM/VH/VHP8-B/VHP847Q.Seq.d/                       1243   0.0
VHP529 (VHP529Q) /CSM/VH/VHP5-B/VHP529Q.Seq.d/                       1243   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ439841) Dictyostelium discoideum cDNA clone:ddv41f19, 3' ...  1257   0.0    1
(BJ436737) Dictyostelium discoideum cDNA clone:ddv32h01, 3' ...  1245   0.0    1
(BJ446821) Dictyostelium discoideum cDNA clone:ddv63o19, 3' ...  1243   0.0    1
(BJ446805) Dictyostelium discoideum cDNA clone:ddv63k21, 3' ...  1243   0.0    1
(BJ446787) Dictyostelium discoideum cDNA clone:ddv63h20, 3' ...  1243   0.0    1
(BJ446785) Dictyostelium discoideum cDNA clone:ddv63g24, 3' ...  1243   0.0    1
(BJ446779) Dictyostelium discoideum cDNA clone:ddv63f24, 3' ...  1243   0.0    1
(BJ446772) Dictyostelium discoideum cDNA clone:ddv63e23, 3' ...  1243   0.0    1
(BJ446716) Dictyostelium discoideum cDNA clone:ddv63j14, 3' ...  1243   0.0    1
(BJ446712) Dictyostelium discoideum cDNA clone:ddv63i13, 3' ...  1243   0.0    1
dna update 2008.10.28
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   255   2e-66
DQ848932_1(DQ848932|pid:none) Scophthalmus maximus clone kbc101 ...   254   4e-66
AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   253   8e-66
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   251   2e-65
A40411(A40411) translation elongation factor eEF-2 - Caenorhabdi...   250   5e-65
BT071866_1(BT071866|pid:none) Salmo salar clone ssal-rgf-501-088...   250   7e-65
BT059038_1(BT059038|pid:none) Salmo salar clone ssal-rgf-521-328...   250   7e-65
BC060707_1(BC060707|pid:none) Mus musculus eukaryotic translatio...   249   1e-64
AK161993_1(AK161993|pid:none) Mus musculus 12 days embryo male w...   249   1e-64
AK298498_1(AK298498|pid:none) Homo sapiens cDNA FLJ56548 complet...   249   1e-64
protein update 2009. 7. 2
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 40.0 %: nuclear
	 12.0 %: cytoskeletal

>> prediction for VHF375 is cyt
5' end seq. ID VHF375F
5' end seq. 
>VHF375F.Seq
AACTCTCGAGTGCAAAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAAT
TCGTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGA
TCACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAA
AGTTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCAC
CATTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGC
TGGTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTT
CAGTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTG
CGNNNNNNNNNN
Length of 5' end seq. 432
3' end seq. ID VHF375Z
3' end seq. 
>VHF375Z.Seq
NNNNNNNNNNAGGTAACGGTGCCAATNTCNTCGTCAATGTCACCAAAGGTGTTCAATATT
TGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTG
TTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTG
ATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTG
CTGAATTAACTGCCTCCCCAACTNTCTTAGAACCAATCTACTTAGTAGAAATTACTGCCC
CAGAAAACGCTATTGGTGGTATTTANTCTGTACTCAATCGTCGTAGAGGTATTGTTATTG
GTGAAGAACGTCGTATCGGTTCCCCATTATTATCTGTCAAAGCTCATTTACCAGTATTAG
AATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAAT
GTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAG
TCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAAT
TCCACGAAAAACTCTAAACAATTTAAGTCGCACTTNAGTTTTCAAAGATAGNAATCAAAT
CTAAAAAAAA
Length of 3' end seq. 670
Connected seq. ID VHF375P
Connected seq. 
>VHF375P.Seq
AACTCTCGAGTGCAAAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAAT
TCGTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGA
TCACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAA
AGTTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCAC
CATTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGC
TGGTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTT
CAGTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTG
CG----------AGGTAACGGTGCCAATNTCNTCGTCAATGTCACCAAAGGTGTTCAATA
TTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGT
TGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACAC
TGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGC
TGCTGAATTAACTGCCTCCCCAACTNTCTTAGAACCAATCTACTTAGTAGAAATTACTGC
CCCAGAAAACGCTATTGGTGGTATTTANTCTGTACTCAATCGTCGTAGAGGTATTGTTAT
TGGTGAAGAACGTCGTATCGGTTCCCCATTATTATCTGTCAAAGCTCATTTACCAGTATT
AGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACA
ATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGA
AGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAA
ATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTNAGTTTTCAAAGATAGNAATCAA
ATCTAAAAAAAA
Length of connected seq. 1082
Full length Seq ID -
Full length Seq. -
Length of full length seq. -