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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHF354
Library VH
(Link to library)
Clone ID VHF354
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VHF354P
(Link to Original site)
Representative DNA sequence 
>VHF354 (VHF354Q) /CSM/VH/VHF3-C/VHF354Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGAXXXXXXXXXXACGAATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGG
TCCAGAAGGTAACGGTGCCAATNTCNTCGTCAATGTCACCAAAGGTGTTCAATATTTGAA
TGAAATTAAAGANTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTG
TGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGC
CATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTANTCTATGCTGCTGA
ATTAACTGCCTCCCCAANTATCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGA
AAACGCTATTGGTGGTATTTANTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGA
AGAACGTCGTATCGGTTCCCCATTATTATCTGTCAAAGCTCATTTACCAGTATTAGAATC
CTTCGGTTTCACCGATGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGT
CTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGC
TTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCA
CGAAAAACTCTAAACAATTTAAGTCGCATCTTTNAGTTTTCAAAGATAGAATCAAATGCT
AAAAAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTE---

---EWDKNDAMNIWSFGPEGNGANXXVNVTKGVQYLNEIKXSFVGAFQWATKEGVVCDEN
MRGIRFNLYDVTLHTDAIHRGGGQIIPTARRVXYAAELTASPXILEPIYLVEITAPENAI
GGIXSVLNRRRGIVIGEERRIGSPLLSVKAHLPVLESFGFTDDLRSHTAGQAFPQCVFDH
WASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*ti*vasxsfqr*nqmlkk
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kklislsvellnpsmsllvtlk---

---tngikmmp*tfglsvqkvtvpxsssmspkvfni*mklkxlslvlsngppkkvlfvmk
t*evsvsifmmspstlmpstevvvkssqllvvxsmlln*lppqxs*nqst**kllpqktl
lvvfxlysivvevlllvknvvsvphyylskliyqy*npsvspmiyvptlpvklshnvssi
iglqlvlstkirkplksl*pperekv*pqksqiltnstknskqfkshlxvfkdrikc*kk

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rslslfp*sy*irqchcw*h*r---

---rmg*k*chehlvfrsrr*rcqxxrqchqrcsife*n*rxfrwcfpmghqrrccl**k
herypfqsl*chpph*chpqrwwsnhpncsscxlcc*inclpxylrtnllsrnycprkry
wwylxctqss*rycyw*rtsyrfpiiicqssftsirilrfhr*ftfphcrssfptmclrs
lgfnwccqqr*esh*srfshqkekrfsprnprs*qiprktlnnlsrifxfskiesnakk

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTE---

---EWDKNDAMNIWSFGPEGNGANXXVNVTKGVQYLNEIKXSFVGAFQWATKEGVVCDEN
MRGIRFNLYDVTLHTDAIHRGGGQIIPTARRVXYAAELTASPXILEPIYLVEITAPENAI
GGIXSVLNRRRGIVIGEERRIGSPLLSVKAHLPVLESFGFTDDLRSHTAGQAFPQCVFDH
WASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*ti*vasxsfqr*nqmlkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF354 (VHF354Q) /CSM/VH/VHF3-C/VHF354Q.Seq.d/                       2476   0.0
VHQ372 (VHQ372Q) /CSM/VH/VHQ3-C/VHQ372Q.Seq.d/                       2418   0.0
VHE779 (VHE779Q) /CSM/VH/VHE7-D/VHE779Q.Seq.d/                       1310   0.0
VHP408 (VHP408Q) /CSM/VH/VHP4-A/VHP408Q.Seq.d/                       1300   0.0
VHK886 (VHK886Q) /CSM/VH/VHK8-D/VHK886Q.Seq.d/                       1300   0.0
VHK821 (VHK821Q) /CSM/VH/VHK8-A/VHK821Q.Seq.d/                       1300   0.0
VHK656 (VHK656Q) /CSM/VH/VHK6-C/VHK656Q.Seq.d/                       1300   0.0
VHK654 (VHK654Q) /CSM/VH/VHK6-C/VHK654Q.Seq.d/                       1300   0.0
VHK634 (VHK634Q) /CSM/VH/VHK6-B/VHK634Q.Seq.d/                       1300   0.0
VHK494 (VHK494Q) /CSM/VH/VHK4-D/VHK494Q.Seq.d/                       1300   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ439801) Dictyostelium discoideum cDNA clone:ddv41l13, 3' ...  1352   0.0    1
(BJ439572) Dictyostelium discoideum cDNA clone:ddv40l20, 3' ...  1296   0.0    1
(BJ439719) Dictyostelium discoideum cDNA clone:ddv41j09, 3' ...  1294   0.0    1
(BJ439582) Dictyostelium discoideum cDNA clone:ddv40n19, 3' ...  1294   0.0    2
(BJ440133) Dictyostelium discoideum cDNA clone:ddv42a22, 3' ...  1289   0.0    1
(BJ439628) Dictyostelium discoideum cDNA clone:ddv41g03, 3' ...  1289   0.0    1
(BJ440045) Dictyostelium discoideum cDNA clone:ddv42p09, 3' ...  1283   0.0    1
(BJ439992) Dictyostelium discoideum cDNA clone:ddv42e09, 3' ...  1283   0.0    1
(BJ439566) Dictyostelium discoideum cDNA clone:ddv40j22, 3' ...  1283   0.0    1
(BJ439422) Dictyostelium discoideum cDNA clone:ddv40l09, 3' ...  1283   0.0    1
dna update 2008.10.28
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   269   1e-70
AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   268   3e-70
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   266   1e-69
BT071866_1(BT071866|pid:none) Salmo salar clone ssal-rgf-501-088...   266   2e-69
BT059038_1(BT059038|pid:none) Salmo salar clone ssal-rgf-521-328...   266   2e-69
BT045031_1(BT045031|pid:none) Salmo salar clone ssal-rgf-510-120...   265   3e-69
BT072304_1(BT072304|pid:none) Salmo salar clone ssal-rgf-520-280...   265   4e-69
BC060707_1(BC060707|pid:none) Mus musculus eukaryotic translatio...   264   6e-69
AK161993_1(AK161993|pid:none) Mus musculus 12 days embryo male w...   264   6e-69
AK298498_1(AK298498|pid:none) Homo sapiens cDNA FLJ56548 complet...   264   6e-69
protein update 2009. 7. 2
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHF354 is cyt
5' end seq. ID VHF354F
5' end seq. 
>VHF354F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGANNNNNNNNNN
Length of 5' end seq. 617
3' end seq. ID VHF354Z
3' end seq. 
>VHF354Z.Seq
NNNNNNNNNNACGAATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAA
GGTAACGGTGCCAATNTCNTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATT
AAAGANTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAA
AACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCAC
AGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTANTCTATGCTGCTGAATTAACT
GCCTCCCCAANTATCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCT
ATTGGTGGTATTTANTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGT
CGTATCGGTTCCCCATTATTATCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGT
TTCACCGATGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGAT
CATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCC
ACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAA
CTCTAAACAATTTAAGTCGCATCTTTNAGTTTTCAAAGATAGAATCAAATGCTAAAAAAA
A
Length of 3' end seq. 721
Connected seq. ID VHF354P
Connected seq. 
>VHF354P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGA----------ACGAATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGG
TCCAGAAGGTAACGGTGCCAATNTCNTCGTCAATGTCACCAAAGGTGTTCAATATTTGAA
TGAAATTAAAGANTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTG
TGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGC
CATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTANTCTATGCTGCTGA
ATTAACTGCCTCCCCAANTATCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGA
AAACGCTATTGGTGGTATTTANTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGA
AGAACGTCGTATCGGTTCCCCATTATTATCTGTCAAAGCTCATTTACCAGTATTAGAATC
CTTCGGTTTCACCGATGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGT
CTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGC
TTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCA
CGAAAAACTCTAAACAATTTAAGTCGCATCTTTNAGTTTTCAAAGATAGAATCAAATGCT
AAAAAAAA
Length of connected seq. 1318
Full length Seq ID -
Full length Seq. -
Length of full length seq. -