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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHF329
Library VH
(Link to library)
Clone ID VHF329
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VHF329P
(Link to Original site)
Representative DNA sequence 
>VHF329 (VHF329Q) /CSM/VH/VHF3-B/VHF329Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATXXXXXXXXXXGGTAACGGTGCCAATCTCATCGTCAATGTCACCAAAGGTGTT
CAATATTTGAATGAAANTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAA
GGTGTTGTTTGTNATNAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTC
CACAGGTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGNTCGTCGTGTACT
CTATGCTGCTGAATTAACTGCCTCCCCAACTNTCTTAGAACCAATGGTACTTAGTAGAAA
TTACTGCCCCAGAAAACGNTATTGGTGGTATTTANTCTGTACTCAATCGTCGTAGAGGTA
TTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTATCTGTCAAAGCTCATTTAC
CAGTATTAGAATCCTTCGGTTTCACCGNTGATTTACGTTCCCACANTGCCGGTCAAGCTT
TCCCACAATGTGTNTTCGATCANTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAG
CCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATC
TTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCA
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTED---

---GNGANLIVNVTKGVQYLNEXKDSFVGAFQWATKEGVVCXXNMRGIRFNLYDVTLHR*
chpqrwwsnhpnxssctlcc*inclpnxlrtngt**kllpqktxlvvfxlysivvevlll
vknvvsvphyylskliyqy*npsvspxiyvptxpvklshnvxsixglqlvlstkirkplk
sl*pperekv*pqksqiltnstknskqfks
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kklislsvellnpsmsllvtlk---

---GNGANLIVNVTKGVQYLNEXKDSFVGAFQWATKEGVVCXXNMRGIRFNLYDVTLHR*
chpqrwwsnhpnxssctlcc*inclpnxlrtngt**kllpqktxlvvfxlysivvevlll
vknvvsvphyylskliyqy*npsvspxiyvptxpvklshnvxsixglqlvlstkirkplk
sl*pperekv*pqksqiltnstknskqfks

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rslslfp*sy*irqchcw*h*r---

---vtvpisssmspkvfni*mkxkilslvlsngppkkvlfvxkt*evsvsifmmspstgd
aihrgggqiiptxrrvlyaaeltasptxlepmvlsrnycprkrywwylxctqss*rycyw
*rtsyrfpiiicqssftsirilrfhr*ftfpxcrssfptmcxrsxgfnwccqqr*esh*s
rfshqkekrfsprnprs*qiprktlnnlsr

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTED---

---*rcqshrqchqrcsife*x*rffrwcfpmghqrrcclxxkherypfqsl*chppqvm
pstevvvkssqlxvvysmlln*lppqls*nqwylveitapenxiggixsvlnrrrgivig
eerrigspllsvkahlpvlesfgftxdlrshxagqafpqcvfdxwasigvvnkdkkatev
alatrkrkglapeipdldkfhekl*ti*va
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF329 (VHF329Q) /CSM/VH/VHF3-B/VHF329Q.Seq.d/                       2296   0.0
VHG874 (VHG874Q) /CSM/VH/VHG8-D/VHG874Q.Seq.d/                       1166   0.0
VHQ476 (VHQ476Q) /CSM/VH/VHQ4-D/VHQ476Q.Seq.d/                       1166   0.0
VHQ473 (VHQ473Q) /CSM/VH/VHQ4-D/VHQ473Q.Seq.d/                       1166   0.0
VHQ469 (VHQ469Q) /CSM/VH/VHQ4-C/VHQ469Q.Seq.d/                       1166   0.0
VHQ463 (VHQ463Q) /CSM/VH/VHQ4-C/VHQ463Q.Seq.d/                       1166   0.0
VHQ453 (VHQ453Q) /CSM/VH/VHQ4-C/VHQ453Q.Seq.d/                       1166   0.0
VHQ446 (VHQ446Q) /CSM/VH/VHQ4-B/VHQ446Q.Seq.d/                       1166   0.0
VHQ441 (VHQ441Q) /CSM/VH/VHQ4-B/VHQ441Q.Seq.d/                       1166   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ439717) Dictyostelium discoideum cDNA clone:ddv41j07, 3' ...  1166   0.0    1
(BJ427897) Dictyostelium discoideum cDNA clone:ddv63p23, 5' ...  1166   0.0    1
(BJ427892) Dictyostelium discoideum cDNA clone:ddv63o22, 5' ...  1166   0.0    1
(BJ427891) Dictyostelium discoideum cDNA clone:ddv63o21, 5' ...  1166   0.0    1
(BJ427888) Dictyostelium discoideum cDNA clone:ddv63n23, 5' ...  1166   0.0    1
(BJ427874) Dictyostelium discoideum cDNA clone:ddv63k23, 5' ...  1166   0.0    1
(BJ427873) Dictyostelium discoideum cDNA clone:ddv63k22, 5' ...  1166   0.0    1
(BJ427872) Dictyostelium discoideum cDNA clone:ddv63k21, 5' ...  1166   0.0    1
(BJ427858) Dictyostelium discoideum cDNA clone:ddv63h20, 5' ...  1166   0.0    1
(BJ427832) Dictyostelium discoideum cDNA clone:ddv63b20, 5' ...  1166   0.0    1
dna update 2008.10.28
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM270011_29(AM270011|pid:none) Aspergillus niger contig An02c016...   264   6e-69
AF248644_1(AF248644|pid:none) Filobasidiella neoformans var. neo...   259   1e-67
(Q6FYA7) RecName: Full=Elongation factor 2;          Short=EF-2;...   258   3e-67
AF083031_87(AF083031|pid:none) Guillardia theta nucleomorph chro...   258   4e-67
AY026074_1(AY026074|pid:none) Monosiga brevicollis elongation fa...   257   7e-67
(Q874B9) RecName: Full=Elongation factor 2;          Short=EF-2;...   257   7e-67
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   256   2e-66
DQ357217_1(DQ357217|pid:none) Leishmania braziliensis elongation...   254   3e-66
AM494972_26(AM494972|pid:none) Leishmania braziliensis chromosom...   254   3e-66
CT005272_19(CT005272|pid:none) Leishmania major strain Friedlin,...   254   4e-66
protein update 2009. 7. 2
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 32.0 %: nuclear
	 20.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for VHF329 is cyt
5' end seq. ID VHF329F
5' end seq. 
>VHF329F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATNNNNNNNNNN
Length of 5' end seq. 618
3' end seq. ID VHF329Z
3' end seq. 
>VHF329Z.Seq
NNNNNNNNNNGGTAACGGTGCCAATCTCATCGTCAATGTCACCAAAGGTGTTCAATATTT
GAATGAAANTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGT
TTGTNATNAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACAGGTG
ATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGNTCGTCGTGTACTCTATGCTG
CTGAATTAACTGCCTCCCCAACTNTCTTAGAACCAATGGTACTTAGTAGAAATTACTGCC
CCAGAAAACGNTATTGGTGGTATTTANTCTGTACTCAATCGTCGTAGAGGTATTGTTATT
GGTGAAGAACGTCGTATCGGTTCCCCATTATTATCTGTCAAAGCTCATTTACCAGTATTA
GAATCCTTCGGTTTCACCGNTGATTTACGTTCCCACANTGCCGGTCAAGCTTTCCCACAA
TGTGTNTTCGATCANTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAA
GTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAA
TTCCACGAAAAACTCTAAACAATTTAAGTCGCA
Length of 3' end seq. 633
Connected seq. ID VHF329P
Connected seq. 
>VHF329P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGAT----------GGTAACGGTGCCAATCTCATCGTCAATGTCACCAAAGGTGTT
CAATATTTGAATGAAANTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAA
GGTGTTGTTTGTNATNAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTC
CACAGGTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGNTCGTCGTGTACT
CTATGCTGCTGAATTAACTGCCTCCCCAACTNTCTTAGAACCAATGGTACTTAGTAGAAA
TTACTGCCCCAGAAAACGNTATTGGTGGTATTTANTCTGTACTCAATCGTCGTAGAGGTA
TTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTATCTGTCAAAGCTCATTTAC
CAGTATTAGAATCCTTCGGTTTCACCGNTGATTTACGTTCCCACANTGCCGGTCAAGCTT
TCCCACAATGTGTNTTCGATCANTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAG
CCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATC
TTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCA
Length of connected seq. 1231
Full length Seq ID -
Full length Seq. -
Length of full length seq. -