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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHF277
Library VH
(Link to library)
Clone ID VHF277
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15541-1
Original site URL
Representative seq. ID VHF277P
(Link to Original site)
Representative DNA sequence 
>VHF277 (VHF277Q) /CSM/VH/VHF2-D/VHF277Q.Seq.d/
AACATACTCCAGTAAAAAATGGATCTTTATCAAGCTTTGGCCAATGCAAATCATCAATCT
GAAAGTGTTCGTTCAGAAGCCGAATCATTTTTAACCAATGCATCACAACAAAACTTTCCA
TTATTTATTCACTCATTAACATTAGAACTTACAAATGAAGAGAGAGATGTACAAATTCGT
CAATTAGCAGGTATTGTTTTAAAGAATAGTATTCACTCTAAAAATAGCGAAAGAAATGAA
ATTTTAATTAAACAATGGGCATCAATTGAACCAAATGCTAGAAACGTCATTAAAAATACT
CTTTTACAAGGTTTAGGATCACCAAAATATGAAGCACGTCACACTGTAGCTATTGTAATT
TCACGTATTGGTCTTATTGAAATTCCATACGCAATGTGGGAAGAATTAATCCCAGCATTA
TTTAAAAACATTGAAAGTGGXXXXXXXXXXAAGAATGTCGATTTTGGTTCCGTAACAAAC
ATTGCAATTGGTATCGTTGGTGATTTAGCTCGTGCTTTTGGTAAGAAATTCTCTGGTGTT
TGCGCTGAAATCGTTCCATTGATCGTTAGTGATCTCACCAATCCAAAACTNTCTATGAAT
GATAAACCAAGTGCTATCAGTTGTCTCTGTGATATCGCCATTAGTGTTGGNGCCGATTTC
ANTCCTTTCCTTCCAATGGTTATGCCAATTCTTCATCAAGCNTCTACCACTGAAATCNAT
GATGAAGACTTTTTAAATGAACTCCGTGAAACTATCTTCCAAACTTACACTGGTATCANT
CAAGGTNTCAAGAGTGATAATCAAATCGAAGAACTCAATCAATACATCAATCCAATGTTA
GACTTTGTTGTACTTATAAATGATGATCCAGAACGTTATGATTCTGTTACTTCTGCTGCC
TTGGCNTTACTTGGTGATTTAGCTCAAGCTATGGGTGATAGTATTAGAAATCTTTTAAAC
AACA
sequence update 2002.10.25
Translated Amino Acid sequence 
nilq*KMDLYQALANANHQSESVRSEAESFLTNASQQNFPLFIHSLTLELTNEERDVQIR
QLAGIVLKNSIHSKNSERNEILIKQWASIEPNARNVIKNTLLQGLGSPKYEARHTVAIVI
SRIGLIEIPYAMWEELIPALFKNIES---

---KNVDFGSVTNIAIGIVGDLARAFGKKFSGVCAEIVPLIVSDLTNPKLSMNDKPSAIS
CLCDIAISVGADFXPFLPMVMPILHQASTTEIXDEDFLNELRETIFQTYTGIXQGXKSDN
QIEELNQYINPMLDFVVLINDDPERYDSVTSAALALLGDLAQAMGDSIRNLLNN
Translated Amino Acid sequence (All Frames) 
Frame A:
nilq*KMDLYQALANANHQSESVRSEAESFLTNASQQNFPLFIHSLTLELTNEERDVQIR
QLAGIVLKNSIHSKNSERNEILIKQWASIEPNARNVIKNTLLQGLGSPKYEARHTVAIVI
SRIGLIEIPYAMWEELIPALFKNIES---

---KNVDFGSVTNIAIGIVGDLARAFGKKFSGVCAEIVPLIVSDLTNPKLSMNDKPSAIS
CLCDIAISVGADFXPFLPMVMPILHQASTTEIXDEDFLNELRETIFQTYTGIXQGXKSDN
QIEELNQYINPMLDFVVLINDDPERYDSVTSAALALLGDLAQAMGDSIRNLLNN

Frame B:
tysskkwifiklwpmqiinlkvfvqkpnhf*pmhhnktfhylfth*h*nlqmkremykfv
n*qvlf*rivftlkiakemkf*lnnghqlnqmletslkilfykv*dhqnmkhvtl*ll*f
hvlvllkfhtqcgkn*sqhylktlkv---

---rmsilvp*qtlqlvslvi*lvllvrnslvfalksfh*slvispiqnxl*minqvlsv
vsvisplvlxpisxlsfqwlcqffikxlplksmmktf*mnsvklsskltlvsxkvsrvii
ksknsintsiqc*tllyl*mmiqnvmilllllpwxylvi*lklwvivleif*tt

Frame C:
htpvkngslssfgqckssi*kcsfrsriifnqcittklsiiyslinirtyk*rerctnss
isrycfke*ysl*k*rkk*nfn*tmgin*tkc*krh*kysftrfritki*stshcsycnf
tywsy*nsirnvgrinpsii*kh*kw---

---ecrfwfrnkhcnwyrw*fsscfw*eilwclr*nrsidr**shqsktxye**tkcyql
sl*yrh*cwxrfxsfpsngyanssssxyh*nx**rlfk*tp*nylpnlhwyxsrxqe**s
nrrtqsihqsnvrlcctyk**srtl*fcyfcclgxtw*fsssyg**y*ksfkq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF277 (VHF277Q) /CSM/VH/VHF2-D/VHF277Q.Seq.d/                       1804   0.0
VHQ406 (VHQ406Q) /CSM/VH/VHQ4-A/VHQ406Q.Seq.d/                        963   0.0
VHQ306 (VHQ306Q) /CSM/VH/VHQ3-A/VHQ306Q.Seq.d/                        963   0.0
VHQ201 (VHQ201Q) /CSM/VH/VHQ2-A/VHQ201Q.Seq.d/                        963   0.0
VHP879 (VHP879Q) /CSM/VH/VHP8-D/VHP879Q.Seq.d/                        963   0.0
VHP826 (VHP826Q) /CSM/VH/VHP8-B/VHP826Q.Seq.d/                        963   0.0
VHP718 (VHP718Q) /CSM/VH/VHP7-A/VHP718Q.Seq.d/                        963   0.0
VHP288 (VHP288Q) /CSM/VH/VHP2-D/VHP288Q.Seq.d/                        963   0.0
VHP264 (VHP264Q) /CSM/VH/VHP2-C/VHP264Q.Seq.d/                        963   0.0
VHP188 (VHP188Q) /CSM/VH/VHP1-D/VHP188Q.Seq.d/                        963   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ439853) Dictyostelium discoideum cDNA clone:ddv41i20, 3' ...   971   0.0    1
(BJ385304) Dictyostelium discoideum cDNA clone:ddc54b17, 3' ...   965   0.0    1
(BJ357485) Dictyostelium discoideum cDNA clone:dda63o17, 3' ...   965   0.0    1
(BJ350751) Dictyostelium discoideum cDNA clone:dda40l23, 3' ...   965   0.0    1
(BJ446574) Dictyostelium discoideum cDNA clone:ddv63l02, 3' ...   963   0.0    1
(BJ446573) Dictyostelium discoideum cDNA clone:ddv63l01, 3' ...   963   0.0    1
(BJ446520) Dictyostelium discoideum cDNA clone:ddv63a02, 3' ...   963   0.0    1
(BJ446504) Dictyostelium discoideum cDNA clone:ddv62n20, 3' ...   963   0.0    1
(BJ446292) Dictyostelium discoideum cDNA clone:ddv62d08, 3' ...   963   0.0    1
(BJ446219) Dictyostelium discoideum cDNA clone:ddv62d05, 3' ...   963   0.0    1
dna update 2008.10.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AK229808_1(AK229808|pid:none) Arabidopsis thaliana mRNA for hypo...   102   2e-20
CP001327_234(CP001327|pid:none) Micromonas sp. RCC299 chromosome...    93   1e-17
CR954202_531(CR954202|pid:none) Ostreococcus tauri strain OTTH05...    91   5e-17
CP000582_69(CP000582|pid:none) Ostreococcus lucimarinus CCE9901 ...    90   1e-16
BC052711_1(BC052711|pid:none) Mus musculus karyopherin (importin...    89   2e-16
(P70168) RecName: Full=Importin subunit beta-1; AltName: Full=Ka...    89   2e-16
AP008209_1176(AP008209|pid:none) Oryza sativa (japonica cultivar...    88   4e-16
AK314780_1(AK314780|pid:none) Homo sapiens cDNA, FLJ95650, highl...    88   5e-16
(Q14974) RecName: Full=Importin subunit beta-1; AltName: Full=Ka...    88   5e-16
AK299145_1(AK299145|pid:none) Homo sapiens cDNA FLJ50625 complet...    88   5e-16
protein update 2009. 7. 2
PSORT 

 psg: 0.76  gvh: 0.42  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 28.0 %: nuclear
	  8.0 %: mitochondrial
	  8.0 %: vesicles of secretory system
	  4.0 %: cytoskeletal

>> prediction for VHF277 is cyt
5' end seq. ID VHF277F
5' end seq. 
>VHF277F.Seq
AACATACTCCAGTAAAAAATGGATCTTTATCAAGCTTTGGCCAATGCAAATCATCAATCT
GAAAGTGTTCGTTCAGAAGCCGAATCATTTTTAACCAATGCATCACAACAAAACTTTCCA
TTATTTATTCACTCATTAACATTAGAACTTACAAATGAAGAGAGAGATGTACAAATTCGT
CAATTAGCAGGTATTGTTTTAAAGAATAGTATTCACTCTAAAAATAGCGAAAGAAATGAA
ATTTTAATTAAACAATGGGCATCAATTGAACCAAATGCTAGAAACGTCATTAAAAATACT
CTTTTACAAGGTTTAGGATCACCAAAATATGAAGCACGTCACACTGTAGCTATTGTAATT
TCACGTATTGGTCTTATTGAAATTCCATACGCAATGTGGGAAGAATTAATCCCAGCATTA
TTTAAAAACATTGAAAGTGGNNNNNNNNNN
Length of 5' end seq. 450
3' end seq. ID VHF277Z
3' end seq. 
>VHF277Z.Seq
NNNNNNNNNNAAGAATGTCGATTTTGGTTCCGTAACAAACATTGCAATTGGTATCGTTGG
TGATTTAGCTCGTGCTTTTGGTAAGAAATTCTCTGGTGTTTGCGCTGAAATCGTTCCATT
GATCGTTAGTGATCTCACCAATCCAAAACTNTCTATGAATGATAAACCAAGTGCTATCAG
TTGTCTCTGTGATATCGCCATTAGTGTTGGNGCCGATTTCANTCCTTTCCTTCCAATGGT
TATGCCAATTCTTCATCAAGCNTCTACCACTGAAATCNATGATGAAGACTTTTTAAATGA
ACTCCGTGAAACTATCTTCCAAACTTACACTGGTATCANTCAAGGTNTCAAGAGTGATAA
TCAAATCGAAGAACTCAATCAATACATCAATCCAATGTTAGACTTTGTTGTACTTATAAA
TGATGATCCAGAACGTTATGATTCTGTTACTTCTGCTGCCTTGGCNTTACTTGGTGATTT
AGCTCAAGCTATGGGTGATAGTATTAGAAATCTTTTAAACAACA
Length of 3' end seq. 524
Connected seq. ID VHF277P
Connected seq. 
>VHF277P.Seq
AACATACTCCAGTAAAAAATGGATCTTTATCAAGCTTTGGCCAATGCAAATCATCAATCT
GAAAGTGTTCGTTCAGAAGCCGAATCATTTTTAACCAATGCATCACAACAAAACTTTCCA
TTATTTATTCACTCATTAACATTAGAACTTACAAATGAAGAGAGAGATGTACAAATTCGT
CAATTAGCAGGTATTGTTTTAAAGAATAGTATTCACTCTAAAAATAGCGAAAGAAATGAA
ATTTTAATTAAACAATGGGCATCAATTGAACCAAATGCTAGAAACGTCATTAAAAATACT
CTTTTACAAGGTTTAGGATCACCAAAATATGAAGCACGTCACACTGTAGCTATTGTAATT
TCACGTATTGGTCTTATTGAAATTCCATACGCAATGTGGGAAGAATTAATCCCAGCATTA
TTTAAAAACATTGAAAGTGG----------AAGAATGTCGATTTTGGTTCCGTAACAAAC
ATTGCAATTGGTATCGTTGGTGATTTAGCTCGTGCTTTTGGTAAGAAATTCTCTGGTGTT
TGCGCTGAAATCGTTCCATTGATCGTTAGTGATCTCACCAATCCAAAACTNTCTATGAAT
GATAAACCAAGTGCTATCAGTTGTCTCTGTGATATCGCCATTAGTGTTGGNGCCGATTTC
ANTCCTTTCCTTCCAATGGTTATGCCAATTCTTCATCAAGCNTCTACCACTGAAATCNAT
GATGAAGACTTTTTAAATGAACTCCGTGAAACTATCTTCCAAACTTACACTGGTATCANT
CAAGGTNTCAAGAGTGATAATCAAATCGAAGAACTCAATCAATACATCAATCCAATGTTA
GACTTTGTTGTACTTATAAATGATGATCCAGAACGTTATGATTCTGTTACTTCTGCTGCC
TTGGCNTTACTTGGTGATTTAGCTCAAGCTATGGGTGATAGTATTAGAAATCTTTTAAAC
AACA
Length of connected seq. 954
Full length Seq ID -
Full length Seq. -
Length of full length seq. -