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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHF273
Library VH
(Link to library)
Clone ID VHF273
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11514-1
Original site URL
Representative seq. ID VHF273P
(Link to Original site)
Representative DNA sequence 
>VHF273 (VHF273Q) /CSM/VH/VHF2-D/VHF273Q.Seq.d/
TTATNATGGACTATGGAAGTTGCAGCTATTGTATCAATTGCATTATTAGATTGGGTTGAT
TTTATATTGATTTGTGCATTATTACTTTTGAATGCAACCATTGGTTTCATTGAAGAGAAT
ACAGCAGGTAATGCAGTAGAGGCTTTAAAGAATTCATTGGTTAGTCAAATCCGTTGTATG
AGAGATGGTGAATGGGTTATGTTACCATCACCAGATCTTGTACCAGGTGATGTTGTAATG
TTAAAGATTGGTGCCATTATTCCAGCCGATTGTAGAGTTATCGAAGCTGAACAAGTTAAA
ATCGATCAATCTTCATTGACAGGTGAATCTTTACCAGTCACTAAAAAGATTGGTGATGAA
GTTTACTCTGGTTCAGCAATGAAACAAGGTGAAGCAAAATGTGTTGTCACTGCAACTGGT
GTAAACACTTTCTTTGGTCGTGCTGCAAATTTAGTACAAGAAACCGAAGGACATGGTCAT
CTTCAAGTTATTCTTAGAAATATTGGTCTCTTTTGTATTAGTTTCATCGCCATTTGGGTA
CTCGTTGAATTATTAGTTGATTTCTTGGGTTACGATGGTTATTGTCATGGTGTTGGTGGT
GGTCGTTGTTTACCATTGAACAATGCTTTGGTTTXXXXXXXXXXGTTTCTATTTAGTAGG
TTCAACCATTGTATTCTTTGCAATCATTCATGATGGTACTTGGTTCCATGATGCTATCAA
TCTTCGTATCTTAACCGATAATGAACTTCGTGGTCTCATCTATCTTCAAGTCTCTATNTC
TGGTCTTGCAACTATTTTCGTATCACGTAGTCAAGGTTTNTCTTACTTTGAACGTCCAGG
TAATCTTGTAATCTTTGCTTTCGTTATGTCTCAAATTGTTGCTACCTTTATTGGTGTCTA
TGGTTTCCGTGGTTATCCACATGATTCCTTTTCCGATAATCCAGATTATCCAGTACATGG
TACAAATTTCCAAGGTTGTGGTTGGGGTTGGGCTGTTTGTGCTTGGATTTGGTGTTTCCT
TTGGTACATTCCAATGGATTTCATTAAATTAGGTGTCACCTATATTCCCCGTGGTAAGAT
TGAACCAATCAATAAAGATGCACTCCGTAAAATNTATGGTTGGTTTGGTAAAGAAATCCC
AAAGGAAGCAACTCAAGTNTNTCATAAAGTTGCTGAACAACAAGCTAAAAGAGATGCTTT
ACAGGCACAAGAAACCCATCATAAATCTGTTGTTACCGATAATAAAGTTTAAAATAACAA
TAATATTTAAATAATGTATAATAATTAGTTTTAATAATTTTANGCAACATAA
sequence update 2002.10.25
Translated Amino Acid sequence 
LXWTMEVAAIVSIALLDWVDFILICALLLLNATIGFIEENTAGNAVEALKNSLVSQIRCM
RDGEWVMLPSPDLVPGDVVMLKIGAIIPADCRVIEAEQVKIDQSSLTGESLPVTKKIGDE
VYSGSAMKQGEAKCVVTATGVNTFFGRAANLVQETEGHGHLQVILRNIGLFCISFIAIWV
LVELLVDFLGYDGYCHGVGGGRCLPLNNALV---

---FYLVGSTIVFFAIIHDGTWFHDAINLRILTDNELRGLIYLQVSXSGLATIFVSRSQG
XSYFERPGNLVIFAFVMSQIVATFIGVYGFRGYPHDSFSDNPDYPVHGTNFQGCGWGWAV
CAWIWCFLWYIPMDFIKLGVTYIPRGKIEPINKDALRKXYGWFGKEIPKEATQVXHKVAE
QQAKRDALQAQETHHKSVVTDNKV*nnnni*imynn*f**fxat*
Translated Amino Acid sequence (All Frames) 
Frame A:
LXWTMEVAAIVSIALLDWVDFILICALLLLNATIGFIEENTAGNAVEALKNSLVSQIRCM
RDGEWVMLPSPDLVPGDVVMLKIGAIIPADCRVIEAEQVKIDQSSLTGESLPVTKKIGDE
VYSGSAMKQGEAKCVVTATGVNTFFGRAANLVQETEGHGHLQVILRNIGLFCISFIAIWV
LVELLVDFLGYDGYCHGVGGGRCLPLNNALV---

---vsi**vqplyslqsfmmvlgsmmlsifvs*pimnfvvssifkslxlvlqlfsyhvvk
vxltlnvqvil*sllslclklllpllvsmvsvvihmipfpiiqiiqymvqiskvvvgvgl
fvlgfgvsfgtfqwisln*vspifpvvrlnqsikmhsvkxmvglvkksqrkqlkxxikll
nnklkemlyrhkkpiinlllpiikfkitiifk*ciiisfnnfxqh

Frame B:
yxglwklqllyqlhy*iglily*fvhyyf*mqplvslkriqqvmq*rl*rihwlvksvv*
emvnglcyhhqilyqvml*c*rlvplfqpivelsklnklksinlh*qvnlyqslkrlvmk
ftlvqq*nkvkqnvlslqlv*tlslvvlqi*ykkpkdmvifklfleilvsfvlvsspfgy
slny*liswvtmvivmvlvvvvvyh*tmlwf---

---flfsrfnhcilcnhs*wylvp*cyqssylnr**tswshlssslyxwscnyfrit*sr
fxll*tsr*scnlcfryvsnccylywclwfpwlst*flfr*srlsstwykfprlwlglgc
lcldlvfplvhsngfh*irchlyspw*d*tnq*rctp*nxwlvw*rnpkgsnssxs*sc*
tts*krcftgtrnps*iccyr**slk*q*ylnnv**lvliilxni

Frame C:
xmdygscsycinciirlg*fyidlciitfecnhwfh*reysr*csrgfkefig*snplye
rw*mgyvtitrsctr*ccnvkdwchyssrl*syrs*ts*nrsifidr*iftsh*kdw**s
llwfsnetr*skmcchcnwckhflwscckfstrnrrtwssssys*kywslly*fhrhlgt
r*iis*flglrwllswcwwwslftieqcfg---

---FYLVGSTIVFFAIIHDGTWFHDAINLRILTDNELRGLIYLQVSXSGLATIFVSRSQG
XSYFERPGNLVIFAFVMSQIVATFIGVYGFRGYPHDSFSDNPDYPVHGTNFQGCGWGWAV
CAWIWCFLWYIPMDFIKLGVTYIPRGKIEPINKDALRKXYGWFGKEIPKEATQVXHKVAE
QQAKRDALQAQETHHKSVVTDNKV*nnnni*imynn*f**fxat*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF273 (VHF273Q) /CSM/VH/VHF2-D/VHF273Q.Seq.d/                       2379   0.0
SLE348 (SLE348Q) /CSM/SL/SLE3-B/SLE348Q.Seq.d/                       1229   0.0
CHQ514 (CHQ514Q) /CSM/CH/CHQ5-A/CHQ514Q.Seq.d/                       1203   0.0
VHN420 (VHN420Q) /CSM/VH/VHN4-A/VHN420Q.Seq.d/                       1183   0.0
VSE676 (VSE676Q) /CSM/VS/VSE6-D/VSE676Q.Seq.d/                       1152   0.0
VHP712 (VHP712Q) /CSM/VH/VHP7-A/VHP712Q.Seq.d/                       1152   0.0
VHN640 (VHN640Q) /CSM/VH/VHN6-B/VHN640Q.Seq.d/                       1152   0.0
VHN486 (VHN486Q) /CSM/VH/VHN4-D/VHN486Q.Seq.d/                       1152   0.0
VHK622 (VHK622Q) /CSM/VH/VHK6-A/VHK622Q.Seq.d/                       1152   0.0
VHJ588 (VHJ588Q) /CSM/VH/VHJ5-D/VHJ588Q.Seq.d/                       1152   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(X98286) D.discoideum mRNA for P-type ATPase.                    1251   0.0    1
(BJ421106) Dictyostelium discoideum cDNA clone:ddv41a20, 5' ...  1251   0.0    1
(BJ370718) Dictyostelium discoideum cDNA clone:ddc55k03, 5' ...  1203   0.0    1
(BJ426085) Dictyostelium discoideum cDNA clone:ddv57h06, 5' ...  1183   0.0    1
(BJ439824) Dictyostelium discoideum cDNA clone:ddv41a20, 3' ...  1146   0.0    1
(BJ367009) Dictyostelium discoideum cDNA clone:ddc41d02, 5' ...  1144   0.0    1
(BJ419028) Dictyostelium discoideum cDNA clone:ddv34e23, 5' ...  1140   0.0    1
(BJ422636) Dictyostelium discoideum cDNA clone:ddv46g15, 5' ...  1136   0.0    1
(AU263289) Dictyostelium discoideum vegetative cDNA clone:VS...  1130   0.0    1
(AU034189) Dictyostelium discoideum slug cDNA, clone SLC237.     1130   0.0    1
dna update 2008.10.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

T30580(T30580) P-type ATPase - slime mold (Dictyostelium discoid...   411   e-113
(P54679) RecName: Full=Probable plasma membrane ATPase;         ...   411   e-113
AL603947_1(AL603947|pid:none) Ustilago maydis gene for predicted...   214   7e-54
AY344962_1(AY344962|pid:none) Phytophthora infestans plasma memb...   209   1e-52
DQ533871_1(DQ533871|pid:none) Phytophthora nicotianae plasma mem...   209   2e-52
(Q7XPY2) RecName: Full=Plasma membrane ATPase;          EC=3.6.3...   205   3e-51
(Q03194) RecName: Full=Plasma membrane ATPase 4;          EC=3.6...   205   3e-51
FB811294_1(FB811294|pid:none) Sequence 30567 from Patent WO20080...   205   3e-51
AF156691_1(AF156691|pid:none) Nicotiana plumbaginifolia plasma m...   204   6e-51
(P83970) RecName: Full=Plasma membrane ATPase;          EC=3.6.3...   204   6e-51
protein update 2009. 7. 2
PSORT 

 psg: 0.75  gvh: 0.81  alm: 0.18  top: 0.40  tms: 0.47  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.30  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 44.0 %: endoplasmic reticulum
	 32.0 %: plasma membrane
	 16.0 %: mitochondrial
	  4.0 %: Golgi
	  4.0 %: vacuolar

>> prediction for VHF273 is end
5' end seq. ID VHF273F
5' end seq. 
>VHF273F.Seq
TTATNATGGACTATGGAAGTTGCAGCTATTGTATCAATTGCATTATTAGATTGGGTTGAT
TTTATATTGATTTGTGCATTATTACTTTTGAATGCAACCATTGGTTTCATTGAAGAGAAT
ACAGCAGGTAATGCAGTAGAGGCTTTAAAGAATTCATTGGTTAGTCAAATCCGTTGTATG
AGAGATGGTGAATGGGTTATGTTACCATCACCAGATCTTGTACCAGGTGATGTTGTAATG
TTAAAGATTGGTGCCATTATTCCAGCCGATTGTAGAGTTATCGAAGCTGAACAAGTTAAA
ATCGATCAATCTTCATTGACAGGTGAATCTTTACCAGTCACTAAAAAGATTGGTGATGAA
GTTTACTCTGGTTCAGCAATGAAACAAGGTGAAGCAAAATGTGTTGTCACTGCAACTGGT
GTAAACACTTTCTTTGGTCGTGCTGCAAATTTAGTACAAGAAACCGAAGGACATGGTCAT
CTTCAAGTTATTCTTAGAAATATTGGTCTCTTTTGTATTAGTTTCATCGCCATTTGGGTA
CTCGTTGAATTATTAGTTGATTTCTTGGGTTACGATGGTTATTGTCATGGTGTTGGTGGT
GGTCGTTGTTTACCATTGAACAATGCTTTGGTTTNNNNNNNNNN
Length of 5' end seq. 644
3' end seq. ID VHF273Z
3' end seq. 
>VHF273Z.Seq
NNNNNNNNNNGTTTCTATTTAGTAGGTTCAACCATTGTATTCTTTGCAATCATTCATGAT
GGTACTTGGTTCCATGATGCTATCAATCTTCGTATCTTAACCGATAATGAACTTCGTGGT
CTCATCTATCTTCAAGTCTCTATNTCTGGTCTTGCAACTATTTTCGTATCACGTAGTCAA
GGTTTNTCTTACTTTGAACGTCCAGGTAATCTTGTAATCTTTGCTTTCGTTATGTCTCAA
ATTGTTGCTACCTTTATTGGTGTCTATGGTTTCCGTGGTTATCCACATGATTCCTTTTCC
GATAATCCAGATTATCCAGTACATGGTACAAATTTCCAAGGTTGTGGTTGGGGTTGGGCT
GTTTGTGCTTGGATTTGGTGTTTCCTTTGGTACATTCCAATGGATTTCATTAAATTAGGT
GTCACCTATATTCCCCGTGGTAAGATTGAACCAATCAATAAAGATGCACTCCGTAAAATN
TATGGTTGGTTTGGTAAAGAAATCCCAAAGGAAGCAACTCAAGTNTNTCATAAAGTTGCT
GAACAACAAGCTAAAAGAGATGCTTTACAGGCACAAGAAACCCATCATAAATCTGTTGTT
ACCGATAATAAAGTTTAAAATAACAATAATATTTAAATAATGTATAATAATTAGTTTTAA
TAATTTTANGCAACATAA
Length of 3' end seq. 678
Connected seq. ID VHF273P
Connected seq. 
>VHF273P.Seq
TTATNATGGACTATGGAAGTTGCAGCTATTGTATCAATTGCATTATTAGATTGGGTTGAT
TTTATATTGATTTGTGCATTATTACTTTTGAATGCAACCATTGGTTTCATTGAAGAGAAT
ACAGCAGGTAATGCAGTAGAGGCTTTAAAGAATTCATTGGTTAGTCAAATCCGTTGTATG
AGAGATGGTGAATGGGTTATGTTACCATCACCAGATCTTGTACCAGGTGATGTTGTAATG
TTAAAGATTGGTGCCATTATTCCAGCCGATTGTAGAGTTATCGAAGCTGAACAAGTTAAA
ATCGATCAATCTTCATTGACAGGTGAATCTTTACCAGTCACTAAAAAGATTGGTGATGAA
GTTTACTCTGGTTCAGCAATGAAACAAGGTGAAGCAAAATGTGTTGTCACTGCAACTGGT
GTAAACACTTTCTTTGGTCGTGCTGCAAATTTAGTACAAGAAACCGAAGGACATGGTCAT
CTTCAAGTTATTCTTAGAAATATTGGTCTCTTTTGTATTAGTTTCATCGCCATTTGGGTA
CTCGTTGAATTATTAGTTGATTTCTTGGGTTACGATGGTTATTGTCATGGTGTTGGTGGT
GGTCGTTGTTTACCATTGAACAATGCTTTGGTTT----------GTTTCTATTTAGTAGG
TTCAACCATTGTATTCTTTGCAATCATTCATGATGGTACTTGGTTCCATGATGCTATCAA
TCTTCGTATCTTAACCGATAATGAACTTCGTGGTCTCATCTATCTTCAAGTCTCTATNTC
TGGTCTTGCAACTATTTTCGTATCACGTAGTCAAGGTTTNTCTTACTTTGAACGTCCAGG
TAATCTTGTAATCTTTGCTTTCGTTATGTCTCAAATTGTTGCTACCTTTATTGGTGTCTA
TGGTTTCCGTGGTTATCCACATGATTCCTTTTCCGATAATCCAGATTATCCAGTACATGG
TACAAATTTCCAAGGTTGTGGTTGGGGTTGGGCTGTTTGTGCTTGGATTTGGTGTTTCCT
TTGGTACATTCCAATGGATTTCATTAAATTAGGTGTCACCTATATTCCCCGTGGTAAGAT
TGAACCAATCAATAAAGATGCACTCCGTAAAATNTATGGTTGGTTTGGTAAAGAAATCCC
AAAGGAAGCAACTCAAGTNTNTCATAAAGTTGCTGAACAACAAGCTAAAAGAGATGCTTT
ACAGGCACAAGAAACCCATCATAAATCTGTTGTTACCGATAATAAAGTTTAAAATAACAA
TAATATTTAAATAATGTATAATAATTAGTTTTAATAATTTTANGCAACATAA
Length of connected seq. 1302
Full length Seq ID -
Full length Seq. -
Length of full length seq. -