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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHF257
Library VH
(Link to library)
Clone ID VHF257
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VHF257P
(Link to Original site)
Representative DNA sequence 
>VHF257 (VHF257Q) /CSM/VH/VHF2-C/VHF257Q.Seq.d/
TGTGTGCGAGTGTAAGAGTAATTAAAACTCTGACGCGTATTGAAAGAAAGAATACTCCAA
AAGATCGTAACTACGGTTACCTTCTGTAAGGAGTGCCCGAATCATGAGAACTCTGTTTCG
AAAGGATTTGCGGTTGAGCACCTAGAATGGGACCCGAAAGGTTGTGAACTATGCCTGAGG
AAGGCGAAGTCAGGGGAAACTCTGATGGAGGCTTGTCGCAATGCTGACGTGCAAATCGCT
TGTCTAACTTGGGTATAGGGGCGAAAGACTAATCGAACAACCTAGTAGCTGGTTCCTTCC
GAAGTTTCCCTCAGGATAGCTGGAGCAGTATTCTAGTTCCATCTTGTAAAGACAATGATT
AGCAGTTTCGGGGGCGTAATGCTCTCAGCTGATTCTCAAACTCTGAACGGGTGGGTATCA
TTTTAATTCACTTAATTGGATTTTAAAATTAAATTGCACATGTGCAATGAAAAATAGGAG
CTCTTAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGTGGGTTGAACCAAATATTGGG
ATAAGACGTCTAACATTCACTAATAGXXXXXXXXXXGCTTTTTGATCNTTCGATGTCGGN
TCTTCTTATCATTGTGAAGCAGAATTCACAAAGTGTTGGATTGTTCACCCACTAACAAGG
AACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGTTGTCAATT
GTTTGCGTAATAGTAGCATGATTTAGTACGAGAGGAACTGTCATGCCGGATCACTGGTCT
GTAGGTTTATTTGACAAAATAGTGACCTGCCGCTACCATCCGTTGGATAATGGCTGAACG
CNTCATAAGTCAGAATCCATTCTAGAAACGCAAACCAAATGCTTTAGAGTGTGAATGTTG
TAGGTAACATTAGGTTGTTGGTGGGGGACCACTTTCAACTTTAAACCATATGATTAATCG
CTGTTACACTGCAGTTTCNTTCCGGTTANTGTGGTGGGTGGATAAATTATAATTTATATC
CTCGTTCCGCTCAACTCTTCGATTGTAGACGACTATCAAATGAACTAGGTGCTGTAAGAC
TTCCGTAGTAGCGTTCAGTTACGAGGGGTTGAGGCTTT
sequence update 2002.10.25
Translated Amino Acid sequence 
cvrv*e*lkl*rvlkerilqkivttvtfckecpnhensvskgfavehlewdpkgcelclr
kaksgetlmeacrnadvqiacltwv*grktnrtt**lvpsevslriagavf*fhlvktmi
ssfggvmlsadsqtlngwvsf*ft*ldfkiklhmcnek*ellvghfw*AELAMWVEPNIG
IRRLTFTN---

---LFDXSMSXLLIIVKQNSQSVGLFTH*qgt*agfrps*drlvlpyccqlfa***hdlv
reelscritgl*vyltk**paatirwimaerxisqnpf*krkpnalececcr*h*vvggg
plstlnhminrcytavsfrlxwwvdkl*fissfrstlrl*ttik*trccktsvvafsyeg
lrl
Translated Amino Acid sequence (All Frames) 
Frame A:
cvrv*e*lkl*rvlkerilqkivttvtfckecpnhensvskgfavehlewdpkgcelclr
kaksgetlmeacrnadvqiacltwv*grktnrtt**lvpsevslriagavf*fhlvktmi
ssfggvmlsadsqtlngwvsf*ft*ldfkiklhmcnek*ellvghfw*AELAMWVEPNIG
IRRLTFTN---

---af*sfdvgssyhceaeftkcwivhpltrnvswv*tvvrqvsftlllsivcviva*fs
trgtvmpdhwsvglfdkivtcryhpldng*txhksesiletqtkcfrv*ml*vtlgcwwg
ttfnfkpyd*sllhcsfxpvxvvgg*iiiyilvplnssivddyqmn*vl*dfrssvqlrg
vea

Frame B:
vcecksn*nsday*kkeyskrs*lrlpsvrsarimrtlfrkdlrlst*ngtrkvvnya*g
rrsqgkl*wrlvamltckslv*lgyrgerlieqpsswflpkfpsg*leqysssil*rq*l
avsga*csqlilkl*tggyhfnslnwilklnctcamknrss*waifgkqnwrcglnqilg
*dv*hsli---

---LFDXSMSXLLIIVKQNSQSVGLFTH*qgt*agfrps*drlvlpyccqlfa***hdlv
reelscritgl*vyltk**paatirwimaerxisqnpf*krkpnalececcr*h*vvggg
plstlnhminrcytavsfrlxwwvdkl*fissfrstlrl*ttik*trccktsvvafsyeg
lrl

Frame C:
casvrviktltrierkntpkdrnygyll*gvpes*elcfericg*aprmgperl*tmpee
gevrgnsdgglsqc*ranrlsnlgigakd*snnlvagsfrsfpqdswssilvpsckdnd*
qfrgrnals*fsnservgiilihligf*n*iahvq*kigalsgpflvsrtgdvg*tkywd
ktsnih**---

---flixrcrxflsl*srihkvldcsptnkerelgldrretg*fyptvvnclrnssmi*y
ernchagslvcrfi*qnsdlplpsvg*wlnas*vrihsrnanqml*svnvvgnirllvgd
hfql*ti*liavtlqfxsgxcggwinynlyprsaqlfdcrrlsnelgavrlp**rsvtrg
*gf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF257 (VHF257Q) /CSM/VH/VHF2-C/VHF257Q.Seq.d/                       2127   0.0
VHJ433 (VHJ433Q) /CSM/VH/VHJ4-B/VHJ433Q.Seq.d/                       1122   0.0
VHH677 (VHH677Q) /CSM/VH/VHH6-D/VHH677Q.Seq.d/                       1122   0.0
VHC874 (VHC874Q) /CSM/VH/VHC8-D/VHC874Q.Seq.d/                       1118   0.0
AHI755 (AHI755Q) /CSM/AH/AHI7-C/AHI755Q.Seq.d/                       1116   0.0
SHE696 (SHE696Q) /CSM/SH/SHE6-D/SHE696Q.Seq.d/                       1108   0.0
CHM596 (CHM596Q) /CSM/CH/CHM5-D/CHM596Q.Seq.d/                       1108   0.0
AHF142 (AHF142Q) /CSM/AH/AHF1-B/AHF142Q.Seq.d/                       1090   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AY171066) Dictyostelium discoideum extrachromosomal palindr...  1120   0.0    1
(BJ423451) Dictyostelium discoideum cDNA clone:ddv49b10, 5' ...  1120   0.0    1
(BJ422724) Dictyostelium discoideum cDNA clone:ddv46i20, 5' ...  1120   0.0    1
(BJ421033) Dictyostelium discoideum cDNA clone:ddv41a16, 5' ...  1120   0.0    1
(BJ419516) Dictyostelium discoideum cDNA clone:ddv36h12, 5' ...  1116   0.0    1
(BJ335031) Dictyostelium discoideum cDNA clone:dda48n13, 5' ...  1114   0.0    1
(BJ368750) Dictyostelium discoideum cDNA clone:ddc47o23, 5' ...  1108   0.0    1
(BJ394149) Dictyostelium discoideum cDNA clone:dds33o24, 5' ...  1106   0.0    1
(X00601) Dictyostelium discoideum ribosomal RNA gene.            1104   0.0    1
(BJ333041) Dictyostelium discoideum cDNA clone:dda41c11, 5' ...  1088   0.0    1
dna update 2008.10.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EU430725_1(EU430725|pid:none) Cupressus sempervirens clone CYPLP...    82   3e-14
AY810980_1(AY810980|pid:none) Schistosoma japonicum clone SJCHGC...    81   8e-14
AP005527_26(AP005527|pid:none) Oryza sativa Japonica Group genom...    60   1e-11
EF091847_1(EF091847|pid:none) Lilium longiflorum hypothetical pr...    71   6e-11
BT087045_1(BT087045|pid:none) Zea mays full-length cDNA clone ZM...    70   1e-10
BT012669_1(BT012669|pid:none) Drosophila melanogaster LD48059 fu...    42   0.039
AL844509_690(AL844509|pid:none) Plasmodium falciparum 3D7 chromo...    42   0.051
CP000497_644(CP000497|pid:none) Pichia stipitis CBS 6054 chromos...    41   0.088
BT058553_1(BT058553|pid:none) Salmo salar clone Contig1810 Cytoc...    39   0.43
AM482385_1(AM482385|pid:none) Vitis vinifera contig VV78X166167....    37   1.7
protein update 2009. 7. 2
PSORT 

 psg: 0.75  gvh: 0.52  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 20.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system

>> prediction for VHF257 is cyt
5' end seq. ID VHF257F
5' end seq. 
>VHF257F.Seq
TGTGTGCGAGTGTAAGAGTAATTAAAACTCTGACGCGTATTGAAAGAAAGAATACTCCAA
AAGATCGTAACTACGGTTACCTTCTGTAAGGAGTGCCCGAATCATGAGAACTCTGTTTCG
AAAGGATTTGCGGTTGAGCACCTAGAATGGGACCCGAAAGGTTGTGAACTATGCCTGAGG
AAGGCGAAGTCAGGGGAAACTCTGATGGAGGCTTGTCGCAATGCTGACGTGCAAATCGCT
TGTCTAACTTGGGTATAGGGGCGAAAGACTAATCGAACAACCTAGTAGCTGGTTCCTTCC
GAAGTTTCCCTCAGGATAGCTGGAGCAGTATTCTAGTTCCATCTTGTAAAGACAATGATT
AGCAGTTTCGGGGGCGTAATGCTCTCAGCTGATTCTCAAACTCTGAACGGGTGGGTATCA
TTTTAATTCACTTAATTGGATTTTAAAATTAAATTGCACATGTGCAATGAAAAATAGGAG
CTCTTAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGTGGGTTGAACCAAATATTGGG
ATAAGACGTCTAACATTCACTAATAGNNNNNNNNNN
Length of 5' end seq. 576
3' end seq. ID VHF257Z
3' end seq. 
>VHF257Z.Seq
NNNNNNNNNNGCTTTTTGATCNTTCGATGTCGGNTCTTCTTATCATTGTGAAGCAGAATT
CACAAAGTGTTGGATTGTTCACCCACTAACAAGGAACGTGAGCTGGGTTTAGACCGTCGT
GAGACAGGTTAGTTTTACCCTACTGTTGTCAATTGTTTGCGTAATAGTAGCATGATTTAG
TACGAGAGGAACTGTCATGCCGGATCACTGGTCTGTAGGTTTATTTGACAAAATAGTGAC
CTGCCGCTACCATCCGTTGGATAATGGCTGAACGCNTCATAAGTCAGAATCCATTCTAGA
AACGCAAACCAAATGCTTTAGAGTGTGAATGTTGTAGGTAACATTAGGTTGTTGGTGGGG
GACCACTTTCAACTTTAAACCATATGATTAATCGCTGTTACACTGCAGTTTCNTTCCGGT
TANTGTGGTGGGTGGATAAATTATAATTTATATCCTCGTTCCGCTCAACTCTTCGATTGT
AGACGACTATCAAATGAACTAGGTGCTGTAAGACTTCCGTAGTAGCGTTCAGTTACGAGG
GGTTGAGGCTTT
Length of 3' end seq. 552
Connected seq. ID VHF257P
Connected seq. 
>VHF257P.Seq
TGTGTGCGAGTGTAAGAGTAATTAAAACTCTGACGCGTATTGAAAGAAAGAATACTCCAA
AAGATCGTAACTACGGTTACCTTCTGTAAGGAGTGCCCGAATCATGAGAACTCTGTTTCG
AAAGGATTTGCGGTTGAGCACCTAGAATGGGACCCGAAAGGTTGTGAACTATGCCTGAGG
AAGGCGAAGTCAGGGGAAACTCTGATGGAGGCTTGTCGCAATGCTGACGTGCAAATCGCT
TGTCTAACTTGGGTATAGGGGCGAAAGACTAATCGAACAACCTAGTAGCTGGTTCCTTCC
GAAGTTTCCCTCAGGATAGCTGGAGCAGTATTCTAGTTCCATCTTGTAAAGACAATGATT
AGCAGTTTCGGGGGCGTAATGCTCTCAGCTGATTCTCAAACTCTGAACGGGTGGGTATCA
TTTTAATTCACTTAATTGGATTTTAAAATTAAATTGCACATGTGCAATGAAAAATAGGAG
CTCTTAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGTGGGTTGAACCAAATATTGGG
ATAAGACGTCTAACATTCACTAATAG----------GCTTTTTGATCNTTCGATGTCGGN
TCTTCTTATCATTGTGAAGCAGAATTCACAAAGTGTTGGATTGTTCACCCACTAACAAGG
AACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGTTGTCAATT
GTTTGCGTAATAGTAGCATGATTTAGTACGAGAGGAACTGTCATGCCGGATCACTGGTCT
GTAGGTTTATTTGACAAAATAGTGACCTGCCGCTACCATCCGTTGGATAATGGCTGAACG
CNTCATAAGTCAGAATCCATTCTAGAAACGCAAACCAAATGCTTTAGAGTGTGAATGTTG
TAGGTAACATTAGGTTGTTGGTGGGGGACCACTTTCAACTTTAAACCATATGATTAATCG
CTGTTACACTGCAGTTTCNTTCCGGTTANTGTGGTGGGTGGATAAATTATAATTTATATC
CTCGTTCCGCTCAACTCTTCGATTGTAGACGACTATCAAATGAACTAGGTGCTGTAAGAC
TTCCGTAGTAGCGTTCAGTTACGAGGGGTTGAGGCTTT
Length of connected seq. 1108
Full length Seq ID -
Full length Seq. -
Length of full length seq. -