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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHF254
Library VH
(Link to library)
Clone ID VHF254
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11124-1
Original site URL
Representative seq. ID VHF254P
(Link to Original site)
Representative DNA sequence 
>VHF254 (VHF254Q) /CSM/VH/VHF2-C/VHF254Q.Seq.d/
ATTTCGAAATAAATTAAATCAAATAAATAAAAAAGATGCCGTCTATTTCACCAAATCTAT
TAGCATCATTACAAGATCATACTAAAAACATTAGAAATATATGTGTGTTGGCACATGTTG
ATCATGGTAAAACCACATTATCCGATTGTTTAATCAGTAGCAATGGTATCATTTCACCAG
AGATGGCTGGTAAATTAAGATATTTAGATTTTCTAGAAGATGAACAAGAAAGAGAAATTA
CAATGAAAGCAAGTGCAATTTCATTATTATTTCAACAACCATCATCATCATCGTCATCAA
ATGATAAAGAATCATTTTTAATTAATTTAATTGATTCACCAGGACATGTGGATTTTTCAA
GTGAAGTTTCAACAGCAGTTAGAATTACAGATGGTGCATTAGTATTGGTTGATGCAGTCG
AAGGTGTTTGTATACAAACTCATGCAGTATTGAAACAAGCTTATCAAGAAAAAGTTAAAC
CATGTTTAGTATTAAATAAAATTGATCGTTTAATACTCGAGTTACATATGACCCCATTGG
AAGCTTATCAACATCTATCAAAAATAATTGAACAAGTTAATGTAATCACTGGTACATTAA
CATCTGAAGAGATCATTCTAAXXXXXXXXXXTACTTCATCAACAATTACATCATCGTCAT
CTACAACAGCAGCAGCAACAGCAACAACAACAAATAATAGTGGTCAGCAATCACCACCAC
TTAAAGAGATTATTACAGTTAAAACAGCAAATAAAAAAGTATCAGTAAAAATACGTGCAA
TTCCATTACCAAAGAATATTACAAATTTAATTGAACAACGTTCACAACAACTTAGAGATC
TTTTCTTGGGTGGTAAGAATACCAATAAAGAATTATCAGATAATAAAATTACAAAGAGAG
AAGCTGAACAAGCAGAAAGAGAAGATTTCCAAAAAGAATTGGAAGAGGAATTAGAAAAAT
CTGGTGGTGATTGGAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
fein*ik*IKKMPSISPNLLASLQDHTKNIRNICVLAHVDHGKTTLSDCLISSNGIISPE
MAGKLRYLDFLEDEQEREITMKASAISLLFQQPSSSSSSNDKESFLINLIDSPGHVDFSS
EVSTAVRITDGALVLVDAVEGVCIQTHAVLKQAYQEKVKPCLVLNKIDRLILELHMTPLE
AYQHLSKIIEQVNVITGTLTSEEIIL---

---TSSTITSSSSTTAAATATTTNNSGQQSPPLKEIITVKTANKKVSVKIRAIPLPKNIT
NLIEQRSQQLRDLFLGGKNTNKELSDNKITKREAEQAEREDFQKELEEELEKSGGDWK
Translated Amino Acid sequence (All Frames) 
Frame A:
isk*iksnk*krcrlfhqiy*hhykiilktleiyvcwhmlimvkphypiv*svamvsfhq
rwlvn*di*if*kmnkkeklq*kqvqfhyyfnnhhhhrhqmiknhf*li*lihqdmwifq
vkfqqqlelqmvh*ywlmqskvfvyklmqy*nklikkklnhv*y*ikliv*yssyi*phw
kliniyqk*lnklm*slvh*hlkrsf*---

---yfinnyiiviynsssnsnnnk**wsaittt*rdyys*nsk*ksiskntcnsitkeyy
kfn*ttfttt*rsflgw*eyq*riir**nykers*tsrkrrfpkrigrgirkiww*le

Frame B:
frnklnqinkkdavyftksisiitrsy*kh*kymcvgtc*sw*nhiirlfnq*qwyhftr
dgw*ikifrfsrr*trkrnyneskcnfiiisttiiiivik**riifn*fn*ftrtcgffk
*sfnss*nyrwcisig*csrrclytnscsietslsrks*tmfsik*n*sfntrvtydpig
slstsiknn*ts*cnhwyini*rdhs---

---TSSTITSSSSTTAAATATTTNNSGQQSPPLKEIITVKTANKKVSVKIRAIPLPKNIT
NLIEQRSQQLRDLFLGGKNTNKELSDNKITKREAEQAEREDFQKELEEELEKSGGDWK

Frame C:
fein*ik*IKKMPSISPNLLASLQDHTKNIRNICVLAHVDHGKTTLSDCLISSNGIISPE
MAGKLRYLDFLEDEQEREITMKASAISLLFQQPSSSSSSNDKESFLINLIDSPGHVDFSS
EVSTAVRITDGALVLVDAVEGVCIQTHAVLKQAYQEKVKPCLVLNKIDRLILELHMTPLE
AYQHLSKIIEQVNVITGTLTSEEIIL---

---lhqqlhhrhlqqqqqqqqqqiivvsnhhhlkrllqlkqqikkyq*kyvqfhyqrilq
i*lnnvhnnleifswvvripiknyqiiklqreklnkqkekiskknwkrn*knlvvig
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF254 (VHF254Q) /CSM/VH/VHF2-C/VHF254Q.Seq.d/                       1810   0.0
VHP880 (VHP880Q) /CSM/VH/VHP8-D/VHP880Q.Seq.d/                       1164   0.0
VHP350 (VHP350Q) /CSM/VH/VHP3-C/VHP350Q.Seq.d/                       1164   0.0
VHL636 (VHL636Q) /CSM/VH/VHL6-B/VHL636Q.Seq.d/                       1164   0.0
VHK862 (VHK862Q) /CSM/VH/VHK8-C/VHK862Q.Seq.d/                       1164   0.0
VHK320 (VHK320Q) /CSM/VH/VHK3-A/VHK320Q.Seq.d/                       1164   0.0
VHG459 (VHG459Q) /CSM/VH/VHG4-C/VHG459Q.Seq.d/                       1164   0.0
VHE141 (VHE141Q) /CSM/VH/VHE1-B/VHE141Q.Seq.d/                       1164   0.0
VHD615 (VHD615Q) /CSM/VH/VHD6-A/VHD615Q.Seq.d/                       1164   0.0
VHC485 (VHC485Q) /CSM/VH/VHC4-D/VHC485Q.Seq.d/                       1164   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ427602) Dictyostelium discoideum cDNA clone:ddv62p20, 5' ...  1164   0.0    1
(BJ427170) Dictyostelium discoideum cDNA clone:ddv61d13, 5' ...  1164   0.0    1
(BJ425012) Dictyostelium discoideum cDNA clone:ddv54g10, 5' ...  1164   0.0    1
(BJ424504) Dictyostelium discoideum cDNA clone:ddv52l16, 5' ...  1164   0.0    1
(BJ424005) Dictyostelium discoideum cDNA clone:ddv51h05, 5' ...  1164   0.0    1
(BJ421705) Dictyostelium discoideum cDNA clone:ddv43f16, 5' ...  1164   0.0    1
(BJ421074) Dictyostelium discoideum cDNA clone:ddv41k14, 5' ...  1164   0.0    1
(BJ420418) Dictyostelium discoideum cDNA clone:ddv39a11, 5' ...  1164   0.0    1
(BJ420079) Dictyostelium discoideum cDNA clone:ddv38m04, 5' ...  1164   0.0    1
(BJ419362) Dictyostelium discoideum cDNA clone:ddv35j22, 5' ...  1164   0.0    1
dna update 2008.10.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q8C0D5) RecName: Full=Elongation factor Tu GTP-binding domain-c...   240   6e-62
BC045616_1(BC045616|pid:none) Mus musculus elongation factor Tu ...   240   6e-62
AK028676_1(AK028676|pid:none) Mus musculus 10 days neonate skin ...   240   6e-62
(Q7Z2Z2) RecName: Full=Elongation factor Tu GTP-binding domain-c...   239   8e-62
CR859273_1(CR859273|pid:none) Pongo abelii mRNA; cDNA DKFZp468B0...   238   3e-61
CU633900_457(CU633900|pid:none) Podospora anserina genomic DNA c...   231   2e-59
BC070807_1(BC070807|pid:none) Xenopus laevis hypothetical protei...   229   1e-58
CR382130_651(CR382130|pid:none) Yarrowia lipolytica strain CLIB1...   228   3e-58
AP007166_425(AP007166|pid:none) Aspergillus oryzae RIB40 genomic...   223   8e-57
(O74945) RecName: Full=Ribosome assembly protein 1;          EC=...   222   2e-56
protein update 2009. 7. 2
PSORT 

 psg: 0.82  gvh: 0.46  alm: 0.35  top: 0.53  tms: 0.00  mit: 0.33  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 32.0 %: cytoplasmic
	 24.0 %: mitochondrial
	  4.0 %: cytoskeletal

>> prediction for VHF254 is nuc
5' end seq. ID VHF254F
5' end seq. 
>VHF254F.Seq
ATTTCGAAATAAATTAAATCAAATAAATAAAAAAGATGCCGTCTATTTCACCAAATCTAT
TAGCATCATTACAAGATCATACTAAAAACATTAGAAATATATGTGTGTTGGCACATGTTG
ATCATGGTAAAACCACATTATCCGATTGTTTAATCAGTAGCAATGGTATCATTTCACCAG
AGATGGCTGGTAAATTAAGATATTTAGATTTTCTAGAAGATGAACAAGAAAGAGAAATTA
CAATGAAAGCAAGTGCAATTTCATTATTATTTCAACAACCATCATCATCATCGTCATCAA
ATGATAAAGAATCATTTTTAATTAATTTAATTGATTCACCAGGACATGTGGATTTTTCAA
GTGAAGTTTCAACAGCAGTTAGAATTACAGATGGTGCATTAGTATTGGTTGATGCAGTCG
AAGGTGTTTGTATACAAACTCATGCAGTATTGAAACAAGCTTATCAAGAAAAAGTTAAAC
CATGTTTAGTATTAAATAAAATTGATCGTTTAATACTCGAGTTACATATGACCCCATTGG
AAGCTTATCAACATCTATCAAAAATAATTGAACAAGTTAATGTAATCACTGGTACATTAA
CATCTGAAGAGATCATTCTAANNNNNNNNNN
Length of 5' end seq. 631
3' end seq. ID VHF254Z
3' end seq. 
>VHF254Z.Seq
NNNNNNNNNNTACTTCATCAACAATTACATCATCGTCATCTACAACAGCAGCAGCAACAG
CAACAACAACAAATAATAGTGGTCAGCAATCACCACCACTTAAAGAGATTATTACAGTTA
AAACAGCAAATAAAAAAGTATCAGTAAAAATACGTGCAATTCCATTACCAAAGAATATTA
CAAATTTAATTGAACAACGTTCACAACAACTTAGAGATCTTTTCTTGGGTGGTAAGAATA
CCAATAAAGAATTATCAGATAATAAAATTACAAAGAGAGAAGCTGAACAAGCAGAAAGAG
AAGATTTCCAAAAAGAATTGGAAGAGGAATTAGAAAAATCTGGTGGTGATTGGAAA
Length of 3' end seq. 356
Connected seq. ID VHF254P
Connected seq. 
>VHF254P.Seq
ATTTCGAAATAAATTAAATCAAATAAATAAAAAAGATGCCGTCTATTTCACCAAATCTAT
TAGCATCATTACAAGATCATACTAAAAACATTAGAAATATATGTGTGTTGGCACATGTTG
ATCATGGTAAAACCACATTATCCGATTGTTTAATCAGTAGCAATGGTATCATTTCACCAG
AGATGGCTGGTAAATTAAGATATTTAGATTTTCTAGAAGATGAACAAGAAAGAGAAATTA
CAATGAAAGCAAGTGCAATTTCATTATTATTTCAACAACCATCATCATCATCGTCATCAA
ATGATAAAGAATCATTTTTAATTAATTTAATTGATTCACCAGGACATGTGGATTTTTCAA
GTGAAGTTTCAACAGCAGTTAGAATTACAGATGGTGCATTAGTATTGGTTGATGCAGTCG
AAGGTGTTTGTATACAAACTCATGCAGTATTGAAACAAGCTTATCAAGAAAAAGTTAAAC
CATGTTTAGTATTAAATAAAATTGATCGTTTAATACTCGAGTTACATATGACCCCATTGG
AAGCTTATCAACATCTATCAAAAATAATTGAACAAGTTAATGTAATCACTGGTACATTAA
CATCTGAAGAGATCATTCTAA----------TACTTCATCAACAATTACATCATCGTCAT
CTACAACAGCAGCAGCAACAGCAACAACAACAAATAATAGTGGTCAGCAATCACCACCAC
TTAAAGAGATTATTACAGTTAAAACAGCAAATAAAAAAGTATCAGTAAAAATACGTGCAA
TTCCATTACCAAAGAATATTACAAATTTAATTGAACAACGTTCACAACAACTTAGAGATC
TTTTCTTGGGTGGTAAGAATACCAATAAAGAATTATCAGATAATAAAATTACAAAGAGAG
AAGCTGAACAAGCAGAAAGAGAAGATTTCCAAAAAGAATTGGAAGAGGAATTAGAAAAAT
CTGGTGGTGATTGGAAA
Length of connected seq. 967
Full length Seq ID -
Full length Seq. -
Length of full length seq. -