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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHF243
Library VH
(Link to library)
Clone ID VHF243
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16423-1
Original site URL
Representative seq. ID VHF243P
(Link to Original site)
Representative DNA sequence 
>VHF243 (VHF243Q) /CSM/VH/VHF2-B/VHF243Q.Seq.d/
AATTTTCATCCACGCGGATAAATTTTTTTAAAGAATTAGTCATTTTATGCCAATTGGAAA
TAACAAAGATAACGATGGAACTACCCTGAATATCTCAGGGTTAACCATCTCTGATAAGAG
TTCGTCAACTTCATCAAACAACAACACAACAACAACAACAACACAAACTAGTGAACCATA
TGTCCCACCATCTCGTAGAAACAAGATGAATAATAGTTCAGGTTCATCAAGAGACGATTA
TCCATCACCATCTGACCGTGATAATCGTGATTCACCATTCAATCGTGACCGTCGTGACGA
TGGTTACAGAGGTGGTAACAATTTTAGGGAAAATAACTCTAATAACAACAACAACAGTAG
AGATAACTACAAAAGTCCATCATTCTCAAGAAACAATAATAGTAATGGTGGTGGTAGTAG
TAGTGGTAGCGGTGGTTGGGATAATGGTCCAAGAGAACAAGGCCCACCAAGAGGTAATAG
TTACTCTTATTCACCAAATAATTCATTCTCTCGTGGTGGTAATCAAGGTGGTTATGGTAA
TAGAGGCGGAAGTGGCAGTAGTTTTGGTAAXXXXXXXXXXTNCNTGTATTCATGGTGATT
TAACTCAACCAGAAAGAGAAAATGCACTTCAATCTTTCNGTTCTTTTGCAACTCCATTCT
TAGTAGCAACTGATATTGCATCAAGAGGTCTCCACATCGGTAATGTTAATTTGGTTATCA
ATTTCGACTTGCCTACCGACATACATATCTACGTTCATCGTATTGGTAGAACCGGAAGAG
CAGGAAAAAAAGGTTTAGCAATTTCTTTNTTCAATGAAAAGAATAAACCAGTTGGTGCTG
AACTTTTGAAATTAATGAAAGCTTCAAATCNAGATATTCCAGATTGGTTTGAAAAGATGG
TACA
sequence update 2002.10.25
Translated Amino Acid sequence 
ifihadkff*RISHFMPIGNNKDNDGTTLNISGLTISDKSSSTSSNNNTTTTTTQTSEPY
VPPSRRNKMNNSSGSSRDDYPSPSDRDNRDSPFNRDRRDDGYRGGNNFRENNSNNNNNSR
DNYKSPSFSRNNNSNGGGSSSGSGGWDNGPREQGPPRGNSYSYSPNNSFSRGGNQGGYGN
RGGSGSSFG---

---XCIHGDLTQPERENALQSFXSFATPFLVATDIASRGLHIGNVNLVINFDLPTDIHIY
VHRIGRTGRAGKKGLAISXFNEKNKPVGAELLKLMKASNXDIPDWFEKMV
Translated Amino Acid sequence (All Frames) 
Frame A:
nfhprg*iflkn*sfyanwk*qr*rwnypeylrvnhl**efvnfikqqhnnnnntn**ti
cptis*kqde**frfikrrlsiti*p**s*ftiqs*ps*rwlqrw*qf*gk*l**qqqq*
r*lqksiilkkq***www***w*rwlg*wskrtrptkr**lllftk*filsww*srwlw*
*rrkwq*fw*---

---xxysw*fnstrkrkctsifxffcnsilssn*ycikrsphr*c*fgyqfrlayrhtyl
rssyw*nrksrkkrfsnffxq*ke*tswc*tfeinesfksrysrlv*kdgt

Frame B:
ifihadkff*RISHFMPIGNNKDNDGTTLNISGLTISDKSSSTSSNNNTTTTTTQTSEPY
VPPSRRNKMNNSSGSSRDDYPSPSDRDNRDSPFNRDRRDDGYRGGNNFRENNSNNNNNSR
DNYKSPSFSRNNNSNGGGSSSGSGGWDNGPREQGPPRGNSYSYSPNNSFSRGGNQGGYGN
RGGSGSSFG---

---XCIHGDLTQPERENALQSFXSFATPFLVATDIASRGLHIGNVNLVINFDLPTDIHIY
VHRIGRTGRAGKKGLAISXFNEKNKPVGAELLKLMKASNXDIPDWFEKMV

Frame C:
fsstrinffkelvilcqleitkitmelp*isqg*pslirvrqlhqtttqqqqqhklvnhm
shhlvetr*iivqvhqetiihhhltviivihhsivtvvtmvtevvtilgkitlittttve
ittkvhhsqetiivmvvvvvvvavvgimvqenkahqevivtlihqiihslvvvikvvmvi
eaevavvlv---

---xvfmvi*lnqkekmhfnlsvllqlhs**qlilhqevstsvmliwlsistclptyist
fivlvepeeqekkv*qflxsmkrinqlvlnf*n**klqixifqiglkrwy
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHN622 (VHN622Q) /CSM/VH/VHN6-A/VHN622Q.Seq.d/                        797   0.0
VHN546 (VHN546Q) /CSM/VH/VHN5-B/VHN546Q.Seq.d/                        797   0.0
VHM632 (VHM632Q) /CSM/VH/VHM6-B/VHM632Q.Seq.d/                        797   0.0
VHM494 (VHM494Q) /CSM/VH/VHM4-D/VHM494Q.Seq.d/                        797   0.0
VHM344 (VHM344Q) /CSM/VH/VHM3-B/VHM344Q.Seq.d/                        797   0.0
VHM184 (VHM184Q) /CSM/VH/VHM1-D/VHM184Q.Seq.d/                        797   0.0
VHL839 (VHL839Q) /CSM/VH/VHL8-B/VHL839Q.Seq.d/                        797   0.0
VHL619 (VHL619Q) /CSM/VH/VHL6-A/VHL619Q.Seq.d/                        797   0.0
VHL166 (VHL166Q) /CSM/VH/VHL1-C/VHL166Q.Seq.d/                        797   0.0
VHK772 (VHK772Q) /CSM/VH/VHK7-C/VHK772Q.Seq.d/                        797   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ422317) Dictyostelium discoideum cDNA clone:ddv45e15, 5' ...   797   0.0    3
(BJ419348) Dictyostelium discoideum cDNA clone:ddv35g21, 5' ...   797   0.0    3
(BJ423112) Dictyostelium discoideum cDNA clone:ddv48i02, 5' ...   797   0.0    3
(BJ423020) Dictyostelium discoideum cDNA clone:ddv47f21, 5' ...   797   0.0    3
(BJ371302) Dictyostelium discoideum cDNA clone:ddc57n10, 5' ...   797   0.0    3
(BJ424922) Dictyostelium discoideum cDNA clone:ddv54e06, 5' ...   797   0.0    3
(BJ424172) Dictyostelium discoideum cDNA clone:ddv51j15, 5' ...   797   0.0    3
(BJ424215) Dictyostelium discoideum cDNA clone:ddv51c19, 5' ...   797   0.0    3
(BJ371007) Dictyostelium discoideum cDNA clone:ddc56e12, 5' ...   797   0.0    3
(BJ369782) Dictyostelium discoideum cDNA clone:ddc51a22, 5' ...   797   0.0    3
dna update 2008.10.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54QS3) RecName: Full=Probable ATP-dependent RNA helicase ddx3;...   210   5e-53
(Q6Z4K6) RecName: Full=DEAD-box ATP-dependent RNA helicase 52B; ...   130   5e-29
GM694515_1(GM694515|pid:none) Sequence 71 from Patent EP1953235....   128   2e-28
BT085991_1(BT085991|pid:none) Zea mays full-length cDNA clone ZM...   127   5e-28
(Q75HJ0) RecName: Full=DEAD-box ATP-dependent RNA helicase 37;  ...   127   7e-28
EU953399_1(EU953399|pid:none) Zea mays clone 1406878 unknown mRNA.    126   9e-28
(Q2R1M8) RecName: Full=DEAD-box ATP-dependent RNA helicase 52C; ...   122   1e-26
AM444101_1(AM444101|pid:none) Vitis vinifera contig VV78X103110....   122   1e-26
BT033920_1(BT033920|pid:none) Zea mays full-length cDNA clone ZM...   122   2e-26
AY062446_1(AY062446|pid:none) Arabidopsis thaliana ATP-dependent...   122   2e-26
protein update 2009. 7. 2
PSORT 

 psg: 0.88  gvh: 0.39  alm: 0.45  top: 0.30  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.11  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 36.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VHF243 is cyt
5' end seq. ID VHF243F
5' end seq. 
>VHF243F.Seq
AATTTTCATCCACGCGGATAAATTTTTTTAAAGAATTAGTCATTTTATGCCAATTGGAAA
TAACAAAGATAACGATGGAACTACCCTGAATATCTCAGGGTTAACCATCTCTGATAAGAG
TTCGTCAACTTCATCAAACAACAACACAACAACAACAACAACACAAACTAGTGAACCATA
TGTCCCACCATCTCGTAGAAACAAGATGAATAATAGTTCAGGTTCATCAAGAGACGATTA
TCCATCACCATCTGACCGTGATAATCGTGATTCACCATTCAATCGTGACCGTCGTGACGA
TGGTTACAGAGGTGGTAACAATTTTAGGGAAAATAACTCTAATAACAACAACAACAGTAG
AGATAACTACAAAAGTCCATCATTCTCAAGAAACAATAATAGTAATGGTGGTGGTAGTAG
TAGTGGTAGCGGTGGTTGGGATAATGGTCCAAGAGAACAAGGCCCACCAAGAGGTAATAG
TTACTCTTATTCACCAAATAATTCATTCTCTCGTGGTGGTAATCAAGGTGGTTATGGTAA
TAGAGGCGGAAGTGGCAGTAGTTTTGGTAANNNNNNNNNN
Length of 5' end seq. 580
3' end seq. ID VHF243Z
3' end seq. 
>VHF243Z.Seq
NNNNNNNNNNTNCNTGTATTCATGGTGATTTAACTCAACCAGAAAGAGAAAATGCACTTC
AATCTTTCNGTTCTTTTGCAACTCCATTCTTAGTAGCAACTGATATTGCATCAAGAGGTC
TCCACATCGGTAATGTTAATTTGGTTATCAATTTCGACTTGCCTACCGACATACATATCT
ACGTTCATCGTATTGGTAGAACCGGAAGAGCAGGAAAAAAAGGTTTAGCAATTTCTTTNT
TCAATGAAAAGAATAAACCAGTTGGTGCTGAACTTTTGAAATTAATGAAAGCTTCAAATC
NAGATATTCCAGATTGGTTTGAAAAGATGGTACA
Length of 3' end seq. 334
Connected seq. ID VHF243P
Connected seq. 
>VHF243P.Seq
AATTTTCATCCACGCGGATAAATTTTTTTAAAGAATTAGTCATTTTATGCCAATTGGAAA
TAACAAAGATAACGATGGAACTACCCTGAATATCTCAGGGTTAACCATCTCTGATAAGAG
TTCGTCAACTTCATCAAACAACAACACAACAACAACAACAACACAAACTAGTGAACCATA
TGTCCCACCATCTCGTAGAAACAAGATGAATAATAGTTCAGGTTCATCAAGAGACGATTA
TCCATCACCATCTGACCGTGATAATCGTGATTCACCATTCAATCGTGACCGTCGTGACGA
TGGTTACAGAGGTGGTAACAATTTTAGGGAAAATAACTCTAATAACAACAACAACAGTAG
AGATAACTACAAAAGTCCATCATTCTCAAGAAACAATAATAGTAATGGTGGTGGTAGTAG
TAGTGGTAGCGGTGGTTGGGATAATGGTCCAAGAGAACAAGGCCCACCAAGAGGTAATAG
TTACTCTTATTCACCAAATAATTCATTCTCTCGTGGTGGTAATCAAGGTGGTTATGGTAA
TAGAGGCGGAAGTGGCAGTAGTTTTGGTAA----------TNCNTGTATTCATGGTGATT
TAACTCAACCAGAAAGAGAAAATGCACTTCAATCTTTCNGTTCTTTTGCAACTCCATTCT
TAGTAGCAACTGATATTGCATCAAGAGGTCTCCACATCGGTAATGTTAATTTGGTTATCA
ATTTCGACTTGCCTACCGACATACATATCTACGTTCATCGTATTGGTAGAACCGGAAGAG
CAGGAAAAAAAGGTTTAGCAATTTCTTTNTTCAATGAAAAGAATAAACCAGTTGGTGCTG
AACTTTTGAAATTAATGAAAGCTTCAAATCNAGATATTCCAGATTGGTTTGAAAAGATGG
TACA
Length of connected seq. 894
Full length Seq ID -
Full length Seq. -
Length of full length seq. -