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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHF231
Library VH
(Link to library)
Clone ID VHF231
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VHF231P
(Link to Original site)
Representative DNA sequence 
>VHF231 (VHF231Q) /CSM/VH/VHF2-B/VHF231Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATAAGGAATTCGGTGATGTCACTGTATCCCXXXXXXXXXXATAAAAATGATGCC
ATGAACATTTGGTCTTTCGGTCCAGAAGGTAACGGTGCCAATTTCNTCGTCAATGTCACC
AAAGGTGTTCAATATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCC
ACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGAT
GTCACCCTCCACACTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGT
CGTGTANTCTATGCTGCTGAATTAACTGCCTCCCCAANTNTTTTAGAACCAATNTACTTA
GTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTANTCTGTANTCAATCGTCGT
ANAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTATCTGTCAAAGCT
CATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGT
CAAGCTTTCCCACAATGTGTNTTCGATCANTGGGCTTCAATTGGTGTTGTCAACAAAGAT
AAGAAAGCCANTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATC
CCAGATCTTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTTGAGTTTTC
AAAGATAGNAATCAAATCTAAAAAAATTTTAAAAAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTEDKEFGDVTVS---

---KNDAMNIWSFGPEGNGANFXVNVTKGVQYLNEIKDSFVGAFQWATKEGVVCDENMRG
IRFNLYDVTLHTDAIHRGGGQIIPTARRVXYAAELTASPXXLEPXYLVEITAPENAIGGI
XSVXNRRXGIVIGEERRIGSPLLSVKAHLPVLESFGFTADLRSHTAGQAFPQCVFDXWAS
IGVVNKDKKAXEVALATRKRKGLAPEIPDLDKFHEKL*ti*val*vfkdxnqi*knfkk
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kklislsvellnpsmsllvtlkirnsvmslyp---

---ikmmp*tfglsvqkvtvpisssmspkvfni*mklkilslvlsngppkkvlfvmkt*e
vsvsifmmspstlmpstevvvkssqllvvxsmlln*lppqxf*nqxt**kllpqktllvv
fxlxsivvxvlllvknvvsvphyylskliyqy*npsvspliyvptlpvklshnvxsixgl
qlvlstkirkpxksl*pperekv*pqksqiltnstknskqfkshfefskixikskkilkk

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rslslfp*sy*irqchcw*h*r*gir*chci---

---*k*chehlvfrsrr*rcqfxrqchqrcsife*n*rffrwcfpmghqrrccl**kher
ypfqsl*chpph*chpqrwwsnhpncsscxlcc*inclpxxfrtnxlsrnycprkrywwy
lxcxqssxrycyw*rtsyrfpiiicqssftsirilrfhr*ftfphcrssfptmcxrsxgf
nwccqqr*esx*srfshqkekrfsprnprs*qiprktlnnlsrtlsfqr*xsnlkkf*kk

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTEDKEFGDVTVS---

---KNDAMNIWSFGPEGNGANFXVNVTKGVQYLNEIKDSFVGAFQWATKEGVVCDENMRG
IRFNLYDVTLHTDAIHRGGGQIIPTARRVXYAAELTASPXXLEPXYLVEITAPENAIGGI
XSVXNRRXGIVIGEERRIGSPLLSVKAHLPVLESFGFTADLRSHTAGQAFPQCVFDXWAS
IGVVNKDKKAXEVALATRKRKGLAPEIPDLDKFHEKL*ti*val*vfkdxnqi*knfkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF231 (VHF231Q) /CSM/VH/VHF2-B/VHF231Q.Seq.d/                       2454   0.0
SHF640 (SHF640Q) /CSM/SH/SHF6-B/SHF640Q.Seq.d/                       1259   0.0
VHP487 (VHP487Q) /CSM/VH/VHP4-D/VHP487Q.Seq.d/                       1251   0.0
VHO803 (VHO803Q) /CSM/VH/VHO8-A/VHO803Q.Seq.d/                       1251   0.0
VHD139 (VHD139Q) /CSM/VH/VHD1-B/VHD139Q.Seq.d/                       1251   0.0
VHB228 (VHB228Q) /CSM/VH/VHB2-B/VHB228Q.Seq.d/                       1251   0.0
VHA614 (VHA614Q) /CSM/VH/VHA6-A/VHA614Q.Seq.d/                       1251   0.0
VHA101 (VHA101Q) /CSM/VH/VHA1-A/VHA101Q.Seq.d/                       1251   0.0
VHH874 (VHH874Q) /CSM/VH/VHH8-D/VHH874Q.Seq.d/                       1249   0.0
VHP832 (VHP832Q) /CSM/VH/VHP8-B/VHP832Q.Seq.d/                       1249   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ439732) Dictyostelium discoideum cDNA clone:ddv41m08, 3' ...  1302   0.0    1
(BJ406494) Dictyostelium discoideum cDNA clone:dds35o10, 3' ...  1283   0.0    1
(BJ438305) Dictyostelium discoideum cDNA clone:ddv37c18, 3' ...  1275   0.0    1
(BJ436374) Dictyostelium discoideum cDNA clone:ddv31a01, 3' ...  1275   0.0    1
(BJ446473) Dictyostelium discoideum cDNA clone:ddv62h19, 3' ...  1273   0.0    1
(BJ446358) Dictyostelium discoideum cDNA clone:ddv62p08, 3' ...  1273   0.0    1
(BJ446187) Dictyostelium discoideum cDNA clone:ddv61n22, 3' ...  1273   0.0    1
(BJ445933) Dictyostelium discoideum cDNA clone:ddv61i04, 3' ...  1273   0.0    1
(BJ445738) Dictyostelium discoideum cDNA clone:ddv60a17, 3' ...  1273   0.0    1
(BJ445613) Dictyostelium discoideum cDNA clone:ddv60f02, 3' ...  1273   0.0    1
dna update 2008.10.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM270011_29(AM270011|pid:none) Aspergillus niger contig An02c016...   273   1e-71
AF083031_87(AF083031|pid:none) Guillardia theta nucleomorph chro...   265   3e-69
(Q6FYA7) RecName: Full=Elongation factor 2;          Short=EF-2;...   265   3e-69
AF248644_1(AF248644|pid:none) Filobasidiella neoformans var. neo...   264   6e-69
DQ357217_1(DQ357217|pid:none) Leishmania braziliensis elongation...   263   1e-68
AM494972_26(AM494972|pid:none) Leishmania braziliensis chromosom...   263   1e-68
(Q874B9) RecName: Full=Elongation factor 2;          Short=EF-2;...   263   1e-68
CT005272_19(CT005272|pid:none) Leishmania major strain Friedlin,...   263   1e-68
CU638744_366(CU638744|pid:none) Podospora anserina genomic DNA c...   261   4e-68
AB095183_1(AB095183|pid:none) Cyanidioschyzon merolae gene for e...   260   7e-68
protein update 2009. 7. 2
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHF231 is cyt
5' end seq. ID VHF231F
5' end seq. 
>VHF231F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATAAGGAATTCGGTGATGTCACTGTATCCCNNNNNNNNNN
Length of 5' end seq. 646
3' end seq. ID VHF231Z
3' end seq. 
>VHF231Z.Seq
NNNNNNNNNNATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAGGTAACGGT
GCCAATTTCNTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGATTCT
TTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGA
GGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGGTGGT
GGTCAAATCATCCCAACTGCTCGTCGTGTANTCTATGCTGCTGAATTAACTGCCTCCCCA
ANTNTTTTAGAACCAATNTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGT
ATTTANTCTGTANTCAATCGTCGTANAGGTATTGTTATTGGTGAAGAACGTCGTATCGGT
TCCCCATTATTATCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCT
GATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTNTTCGATCANTGGGCT
TCAATTGGTGTTGTCAACAAAGATAAGAAAGCCANTGAAGTCGCTTTAGCCACCAGAAAG
AGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACA
ATTTAAGTCGCACTTTGAGTTTTCAAAGATAGNAATCAAATCTAAAAAAATTTTAAAAAA
AA
Length of 3' end seq. 722
Connected seq. ID VHF231P
Connected seq. 
>VHF231P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATAAGGAATTCGGTGATGTCACTGTATCCC----------ATAAAAATGATGCC
ATGAACATTTGGTCTTTCGGTCCAGAAGGTAACGGTGCCAATTTCNTCGTCAATGTCACC
AAAGGTGTTCAATATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCC
ACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGAT
GTCACCCTCCACACTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGT
CGTGTANTCTATGCTGCTGAATTAACTGCCTCCCCAANTNTTTTAGAACCAATNTACTTA
GTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTANTCTGTANTCAATCGTCGT
ANAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTATCTGTCAAAGCT
CATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGT
CAAGCTTTCCCACAATGTGTNTTCGATCANTGGGCTTCAATTGGTGTTGTCAACAAAGAT
AAGAAAGCCANTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATC
CCAGATCTTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTTGAGTTTTC
AAAGATAGNAATCAAATCTAAAAAAATTTTAAAAAAAA
Length of connected seq. 1348
Full length Seq ID -
Full length Seq. -
Length of full length seq. -