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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHF221
Library VH
(Link to library)
Clone ID VHF221
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VHF221P
(Link to Original site)
Representative DNA sequence 
>VHF221 (VHF221Q) /CSM/VH/VHF2-A/VHF221Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAXXXXXXXXXXATGAATT
AACTGCCTCCCCAANTATCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAA
CGCTATTGGTGGTATTTAATCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGA
ACGTCGTATCGGTTCCCCATTATTATCTGTCAAAGCTCATTTACCAGTATTAGAATCNTT
CGGTTTCACCGATGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTT
CGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCNCTGAAGTCGCTTT
AGCCNCCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGA
AAAACTCTAAACAATTTAAGTCGCACTTGAGTTTTCAAAG
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLL---

---ELTASPXILEPIYLVEITAPENAIGGI*svlnrrrgivigeerrigspllsvkahlp
vlesfgftddlrshtagqafpqcvfdhwasigvvnkdkkaxevalaxrkrkglapeipdl
dkfhekl*ti*valefsk
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*---

---mn*lppqxs*nqst**kllpqktllvvfnlysivvevlllvknvvsvphyylskliy
qy*nxsvspmiyvptlpvklshnvssiiglqlvlstkirkplksl*pperekv*pqksqi
ltnstknskqfkshlsfq

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflir---

---*inclpxylrtnllsrnycprkrywwylictqss*rycyw*rtsyrfpiiicqssft
sirixrfhr*ftfphcrssfptmclrslgfnwccqqr*esx*srfsxqkekrfsprnprs
*qiprktlnnlsrt*vfk

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLL---

---ELTASPXILEPIYLVEITAPENAIGGI*svlnrrrgivigeerrigspllsvkahlp
vlesfgftddlrshtagqafpqcvfdhwasigvvnkdkkaxevalaxrkrkglapeipdl
dkfhekl*ti*valefsk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHP874 (VHP874Q) /CSM/modify/h12/F/treated/VHP874Q.Seq.d/             997   0.0
VHH874 (VHH874Q) /CSM/VH/VHH8-D/VHH874Q.Seq.d/                        997   0.0
VHG874 (VHG874Q) /CSM/VH/VHG8-D/VHG874Q.Seq.d/                        997   0.0
VHP874 (VHP874Q) /CSM/modify/h12/F/treated/VHP874Q.Seq.d/             997   0.0
VHH874 (VHH874Q) /CSM/VH/VHH8-D/VHH874Q.Seq.d/                        997   0.0
VHG874 (VHG874Q) /CSM/VH/VHG8-D/VHG874Q.Seq.d/                        997   0.0
VHQ476 (VHQ476Q) /CSM/VH/VHQ4-D/VHQ476Q.Seq.d/                        997   0.0
VHQ473 (VHQ473Q) /CSM/VH/VHQ4-D/VHQ473Q.Seq.d/                        997   0.0
VHQ469 (VHQ469Q) /CSM/VH/VHQ4-C/VHQ469Q.Seq.d/                        997   0.0
VHQ463 (VHQ463Q) /CSM/VH/VHQ4-C/VHQ463Q.Seq.d/                        997   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(M26017) D.discoideum elongation factor 2 mRNA, complete cds.     995   0.0    4
(BJ427897) Dictyostelium discoideum cDNA clone:ddv63p23, 5' ...   997   0.0    1
(BJ427892) Dictyostelium discoideum cDNA clone:ddv63o22, 5' ...   997   0.0    1
(BJ427891) Dictyostelium discoideum cDNA clone:ddv63o21, 5' ...   997   0.0    1
(BJ427889) Dictyostelium discoideum cDNA clone:ddv63o19, 5' ...   997   0.0    1
(BJ427888) Dictyostelium discoideum cDNA clone:ddv63n23, 5' ...   997   0.0    1
(BJ427882) Dictyostelium discoideum cDNA clone:ddv63m22, 5' ...   997   0.0    1
(BJ427875) Dictyostelium discoideum cDNA clone:ddv63k24, 5' ...   997   0.0    1
(BJ427874) Dictyostelium discoideum cDNA clone:ddv63k23, 5' ...   997   0.0    1
(BJ427873) Dictyostelium discoideum cDNA clone:ddv63k22, 5' ...   997   0.0    1
dna update 2008.10.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM270011_29(AM270011|pid:none) Aspergillus niger contig An02c016...   235   2e-60
AF083031_87(AF083031|pid:none) Guillardia theta nucleomorph chro...   232   1e-59
AF248644_1(AF248644|pid:none) Filobasidiella neoformans var. neo...   231   4e-59
AY026074_1(AY026074|pid:none) Monosiga brevicollis elongation fa...   228   2e-58
(Q6FYA7) RecName: Full=Elongation factor 2;          Short=EF-2;...   228   2e-58
(Q874B9) RecName: Full=Elongation factor 2;          Short=EF-2;...   228   2e-58
DQ357217_1(DQ357217|pid:none) Leishmania braziliensis elongation...   227   5e-58
AM494972_26(AM494972|pid:none) Leishmania braziliensis chromosom...   227   5e-58
AY618550_1(AY618550|pid:none) Trypanosoma cruzi elongation facto...   226   7e-58
CT005272_19(CT005272|pid:none) Leishmania major strain Friedlin,...   226   7e-58
protein update 2009. 7. 2
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 32.0 %: nuclear
	 20.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for VHF221 is cyt
5' end seq. ID VHF221F
5' end seq. 
>VHF221F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGANNNNNNNNNN
Length of 5' end seq. 533
3' end seq. ID VHF221Z
3' end seq. 
>VHF221Z.Seq
NNNNNNNNNNATGAATTAACTGCCTCCCCAANTATCTTAGAACCAATCTACTTAGTAGAA
ATTACTGCCCCAGAAAACGCTATTGGTGGTATTTAATCTGTACTCAATCGTCGTAGAGGT
ATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTATCTGTCAAAGCTCATTTA
CCAGTATTAGAATCNTTCGGTTTCACCGATGATTTACGTTCCCACACTGCCGGTCAAGCT
TTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAA
GCCNCTGAAGTCGCTTTAGCCNCCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGAT
CTTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTGAGTTTTCAAAG
Length of 3' end seq. 417
Connected seq. ID VHF221P
Connected seq. 
>VHF221P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGA----------ATGAATT
AACTGCCTCCCCAANTATCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAA
CGCTATTGGTGGTATTTAATCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGA
ACGTCGTATCGGTTCCCCATTATTATCTGTCAAAGCTCATTTACCAGTATTAGAATCNTT
CGGTTTCACCGATGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTT
CGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCNCTGAAGTCGCTTT
AGCCNCCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGA
AAAACTCTAAACAATTTAAGTCGCACTTGAGTTTTCAAAG
Length of connected seq. 930
Full length Seq ID -
Full length Seq. -
Length of full length seq. -