>AFN775 (AFN775Q) /CSM/AF/AFN7-D/AFN775Q.Seq.d/
AAAATTTTTTATTTTGCATGATAAAGCTGTTCATTATTTTTTCATTTTTTTTTTTATTTT
TTAGGGNCATAATTCATCAAGCAAAACAACCACAACANGAACAACAACAACAAANGTCAA
TTTTAAAAAGTTTTATTGAAGTTAGNGAAGATTCACACTTTCCAATTCAAAATCTTCCAT
ATGGNGTCTTTAAACCAACCCTAAATGATCAAGCTAGAATTGGNGTTGCTATTGGNGATT
TCGTTTGNGATTTATCAGTTTTAGCAGATTTAAAACTTTTTGATGGTAAATTAAAAGATN
CTAAAGTTTTTCATCAAGAAAATTTAAATTCATTTATGTCATTAGGTAAAGAATTATGGA
GNGAAGCTAGAAAAACCATTCAAAATTTATTATCATCAGAAACATCANCTNTTAGAGATA
ATAAAGAGTNTAGAGAAAAGATNTTTCATTCAATTTCAAGNGTAACAATGTTATTACCAG
CAAGAATTGGAGATTATNCTGATTTCTATGCATCAAAAGAACATGCAACCAATGTTGGTN
TTATGTTTAGAGXXXXXXXXXXTTAATCCATGTAGATTATTGGATTTTGAATTGGAGATG
GGCGCTTTGATTGGAGGAGAATCAACCAAATTGGGTGAACCAATTTCAATTGAGTCTGCA
AAAGATCATATCTTTGGTTTGGTACTTTTGAATGATTGGAGTGCAAGAGATATCCAAAAA
TGGGAATACGTTCCATTGGGTCCATTCTTGGCAAAGAATTTCGGCTCAACTATATCACCA
TGGGTTGTTACAATGGAGGCATTGCAACCATTTGCTACAAAACCACCAACTCAAGATCCA
CAACCAATGAAATATCTTCAAGAACAAGGTAATACTACCTTTGATATTGAATTATCAGTT
AGTATTAAATCTCCAAAAATGTCAAAACCTCATAAGGTTTCAACTTCAAATTTGAAATAC
ATGTATTGGACACTCACTCAACAATTGGCTCATCATACTGTAAATGGTTGTAATATGAAT
GCTGGTGATTTACTTGGTACTGGTACAATTAGTGGTCCAACTGAAGATAGCTATGGTTCA
ATGTTGGAACTCTCTTGAAAAGGTAGTAAAGTGGTCTCATTAGGTACTGAAACAAATGAA
GAAAGAAAATTCATTCAAGATGGTGATTCTGTTATCTTATCTGGTCTTTGTAAAGGTAAT
GGTTATCAAATTGGTTTTGGTAATTGTGAAGGTACAATTTTACCAGCTGATAAACGTCAA
TAAAAAAAAATTAATTAAATAAATAAATAAATAAAGAAAACTATTTTTAAAT

NFLFCMIKLFIIFSFFFLFFRXIIHQAKQPQXEQQQQXSILKSFIEVXEDSHFPIQNLPY
GVFKPTLNDQARIGVAIGDFVXDLSVLADLKLFDGKLKDXKVFHQENLNSFMSLGKELWX
EARKTIQNLLSSETSXXRDNKEXREKXFHSISXVTMLLPARIGDYXDFYASKEHATNVGX
MFR---

---NPCRLLDFELEMGALIGGESTKLGEPISIESAKDHIFGLVLLNDWSARDIQKWEYVP
LGPFLAKNFGSTISPWVVTMEALQPFATKPPTQDPQPMKYLQEQGNTTFDIELSVSIKSP
KMSKPHKVSTSNLKYMYWTLTQQLAHHTVNGCNMNAGDLLGTGTISGPTEDSYGSMLELS
*kgskvvslgtetneerkfiqdgdsvilsglckgngyqigfgncegtilpadkrq*kkin
*ink*ikktifk

Frame A:
kifyfa**scslffhfffyflgx*fikqnnhnxnnnnkxqf*kvllklxkihtfqfkifh
mxslnqp*miklelxlllxisfxiyqf*qi*nflmvn*kxlkffikki*ihlch*vknyg
xklekpfkiyyhqkhxlleiiksxekrxfiqfqx*qcyyqqeleixlismhqknmqpmlv
lcle---

---lihvdywilnwrwal*leenqpnwvnqfqlslqkiislvwyf*migvqeiskngntf
hwvhswqrisaqlyhhgllqwrhcnhllqnhqlkihnq*nifknkvilplilnyqlvlnl
qkcqnlirfqlqi*ntcighslnnwliil*mvvi*mlviylvlvqlvvqlkiamvqcwns
lekvvkwsh*vlkqmkkensfkmvillsylvfvkvmviklvlvivkvqfyqlinvnkkkl
ik*ink*rklfln

Frame B:
kffilhdkavhyffifffif*ghnssskttttxttttxvnfkkfy*s*xrftlsnskssi
wxl*tnpk*ss*nwxcywxfrlxfisfsrfktf*w*ikrx*sfssrkfkfiyvir*rime
xs*knhskfiiirnixx*r**rv*rkdxsfnfkxnnvitsknwrlx*flcikrtcnqcwx
yv*---

---*sm*iigf*igdgrfdwrrinqig*tnfn*vckrsylwfgtfe*leckrypkmgirs
igsilgkefrlnyitmgcynggiaticykttnsrsttneissrtr*yyl*y*iis*y*is
knvkts*gfnfkfeihvldthstigssyckwl*yecw*ftwywyn*wsn*r*lwfnvgtl
lkr**sgliry*nk*rkkihsrw*fcyliwsl*r*wlsnwfw*l*rynfts**tsikkn*
lnk*inkenyf*

Frame C:
NFLFCMIKLFIIFSFFFLFFRXIIHQAKQPQXEQQQQXSILKSFIEVXEDSHFPIQNLPY
GVFKPTLNDQARIGVAIGDFVXDLSVLADLKLFDGKLKDXKVFHQENLNSFMSLGKELWX
EARKTIQNLLSSETSXXRDNKEXREKXFHSISXVTMLLPARIGDYXDFYASKEHATNVGX
MFR---

---NPCRLLDFELEMGALIGGESTKLGEPISIESAKDHIFGLVLLNDWSARDIQKWEYVP
LGPFLAKNFGSTISPWVVTMEALQPFATKPPTQDPQPMKYLQEQGNTTFDIELSVSIKSP
KMSKPHKVSTSNLKYMYWTLTQQLAHHTVNGCNMNAGDLLGTGTISGPTEDSYGSMLELS
*kgskvvslgtetneerkfiqdgdsvilsglckgngyqigfgncegtilpadkrq*kkin
*ink*ikktifk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFN775 (AFN775Q) /CSM/AF/AFN7-D/AFN775Q.Seq.d/                       2123   0.0
SFD819 (SFD819Q) /CSM/SF/SFD8-A/SFD819Q.Seq.d/                       1372   0.0
AFE706 (AFE706Q) /CSM/AF/AFE7-A/AFE706Q.Seq.d/                       1362   0.0
CFA612 (CFA612Q) /CSM/CF/CFA6-A/CFA612Q.Seq.d/                       1340   0.0
CHD537 (CHD537Q) /CSM/CH/CHD5-B/CHD537Q.Seq.d/                       1332   0.0
SFF638 (SFF638Q) /CSM/SF/SFF6-B/SFF638Q.Seq.d/                       1310   0.0
SFB270 (SFB270Q) /CSM/SF/SFB2-C/SFB270Q.Seq.d/                       1298   0.0
CFE715 (CFE715Q) /CSM/CF/CFE7-A/CFE715Q.Seq.d/                       1289   0.0
AFG455 (AFG455Q) /CSM/AF/AFG4-C/AFG455Q.Seq.d/                       1287   0.0
AFO620 (AFO620Q) /CSM/AF/AFO6-A/AFO620Q.Seq.d/                       1285   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BM774584|BM774584.1 ra95c11.y1 Meloidogyne incognita egg pAMP1 Bird Meloidogyne incognita cDNA 5' similar to TR:Q94272 Q94272 SIMILAR TO FUMARYLACETOACETASE. [1] ;, mRNA sequence.    64  1e-12  2
BI504681|BI504681.1 BB170011A20F01 Bee Brain Normalized/Subtracted Library, BB17 Apis mellifera cDNA clone BB170011A20F01 5', mRNA sequence.    64  6e-08  2
AW827814|AW827814.1 ra59b10.y1 Bird-Rao Meloidogyne incognita J2 Meloidogyne incognita cDNA 5' similar to WP:K10C2.4 CE04750 FUMARYLACETOACETASE ;, mRNA sequence.    48  5e-07  3
AW871572|AW871572.1 ra79e06.y1 Bird-Rao Meloidogyne incognita J2 Meloidogyne incognita cDNA 5' similar to WP:K10C2.4 CE04750 FUMARYLACETOACETASE ;, mRNA sequence.    48  5e-07  3
CF440839|CF440839.1 EST677184 normalized cDNA library of onion Allium cepa cDNA clone ACAFM72, mRNA sequence.    64  5e-06  1
CF446374|CF446374.1 EST682719 normalized cDNA library of onion Allium cepa cDNA clone ACAHY76, mRNA sequence.    64  5e-06  1
BI933163|BI933163.1 EST553052 tomato flower, 8 mm to preanthesis buds Lycopersicon esculentum cDNA clone cTOC25I18 5' end, mRNA sequence.    56  1e-05  2
BI422229|BI422229.1 EST532895 tomato callus, TAMU Lycopersicon esculentum cDNA clone cLEC69O22 5' end, mRNA sequence.    56  1e-05  2
BM410976|BM410976.1 EST585303 tomato breaker fruit Lycopersicon esculentum cDNA clone cLEG55C6 5' end, mRNA sequence.    56  1e-05  2
CB377955|CB377955.1 rq05b07.y1 Heterodera glycines J3 Heterodera glycines cDNA 5' similar to TR:Q94272 Q94272 SIMILAR TO FUMARYLACETOACETASE. [1] ;, mRNA sequence.    62  2e-05  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q1ZXQ1) RecName: Full=Fumarylacetoacetase;          Short=FAA; ...   474   e-132
(A5PKH3) RecName: Full=Fumarylacetoacetase;          Short=FAA; ...   292   2e-77
AY891819_1(AY891819|pid:none) Synthetic construct Homo sapiens c...   290   1e-76
(P16930) RecName: Full=Fumarylacetoacetase;          Short=FAA; ...   290   1e-76
T25813(T25813) hypothetical protein K10C2.4 - Caenorhabditis ele...   285   2e-75
CP000159_2302(CP000159|pid:none) Salinibacter ruber DSM 13855, c...   283   9e-75
EF144286_1(EF144286|pid:none) Populus trichocarpa clone PX0015_F...   283   1e-74
BC157696_1(BC157696|pid:none) Xenopus tropicalis hypothetical pr...   282   2e-74
BC044366_1(BC044366|pid:none) Danio rerio fumarylacetoacetate hy...   281   3e-74
BC165458_1(BC165458|pid:none) Danio rerio fumarylacetoacetate hy...   281   3e-74

 psg: 1.02  gvh: 0.48  alm: 0.20  top: 0.50  tms: 0.07  mit: 0.32  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: endoplasmic reticulum
	 20.0 %: nuclear
	 16.0 %: cytoplasmic
	 16.0 %: mitochondrial
	  8.0 %: plasma membrane
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system
	  4.0 %: extracellular, including cell wall

>> prediction for AFN775 is end

>AFN775F.Seq
AAAATTTTTTATTTTGCATGATAAAGCTGTTCATTATTTTTTCATTTTTTTTTTTATTTT
TTAGGGNCATAATTCATCAAGCAAAACAACCACAACANGAACAACAACAACAAANGTCAA
TTTTAAAAAGTTTTATTGAAGTTAGNGAAGATTCACACTTTCCAATTCAAAATCTTCCAT
ATGGNGTCTTTAAACCAACCCTAAATGATCAAGCTAGAATTGGNGTTGCTATTGGNGATT
TCGTTTGNGATTTATCAGTTTTAGCAGATTTAAAACTTTTTGATGGTAAATTAAAAGATN
CTAAAGTTTTTCATCAAGAAAATTTAAATTCATTTATGTCATTAGGTAAAGAATTATGGA
GNGAAGCTAGAAAAACCATTCAAAATTTATTATCATCAGAAACATCANCTNTTAGAGATA
ATAAAGAGTNTAGAGAAAAGATNTTTCATTCAATTTCAAGNGTAACAATGTTATTACCAG
CAAGAATTGGAGATTATNCTGATTTCTATGCATCAAAAGAACATGCAACCAATGTTGGTN
TTATGTTTAGAG----------

>AFN775Z.Seq
----------TTAATCCATGTAGATTATTGGATTTTGAATTGGAGATGGGCGCTTTGATT
GGAGGAGAATCAACCAAATTGGGTGAACCAATTTCAATTGAGTCTGCAAAAGATCATATC
TTTGGTTTGGTACTTTTGAATGATTGGAGTGCAAGAGATATCCAAAAATGGGAATACGTT
CCATTGGGTCCATTCTTGGCAAAGAATTTCGGCTCAACTATATCACCATGGGTTGTTACA
ATGGAGGCATTGCAACCATTTGCTACAAAACCACCAACTCAAGATCCACAACCAATGAAA
TATCTTCAAGAACAAGGTAATACTACCTTTGATATTGAATTATCAGTTAGTATTAAATCT
CCAAAAATGTCAAAACCTCATAAGGTTTCAACTTCAAATTTGAAATACATGTATTGGACA
CTCACTCAACAATTGGCTCATCATACTGTAAATGGTTGTAATATGAATGCTGGTGATTTA
CTTGGTACTGGTACAATTAGTGGTCCAACTGAAGATAGCTATGGTTCAATGTTGGAACTC
TCTTGAAAAGGTAGTAAAGTGGTCTCATTAGGTACTGAAACAAATGAAGAAAGAAAATTC
ATTCAAGATGGTGATTCTGTTATCTTATCTGGTCTTTGTAAAGGTAATGGTTATCAAATT
GGTTTTGGTAATTGTGAAGGTACAATTTTACCAGCTGATAAACGTCAATAAAAAAAAATT
AATTAAATAAATAAATAAATAAAGAAAACTATTTTTAAAT

>AFN775P.Seq
AAAATTTTTTATTTTGCATGATAAAGCTGTTCATTATTTTTTCATTTTTTTTTTTATTTT
TTAGGGNCATAATTCATCAAGCAAAACAACCACAACANGAACAACAACAACAAANGTCAA
TTTTAAAAAGTTTTATTGAAGTTAGNGAAGATTCACACTTTCCAATTCAAAATCTTCCAT
ATGGNGTCTTTAAACCAACCCTAAATGATCAAGCTAGAATTGGNGTTGCTATTGGNGATT
TCGTTTGNGATTTATCAGTTTTAGCAGATTTAAAACTTTTTGATGGTAAATTAAAAGATN
CTAAAGTTTTTCATCAAGAAAATTTAAATTCATTTATGTCATTAGGTAAAGAATTATGGA
GNGAAGCTAGAAAAACCATTCAAAATTTATTATCATCAGAAACATCANCTNTTAGAGATA
ATAAAGAGTNTAGAGAAAAGATNTTTCATTCAATTTCAAGNGTAACAATGTTATTACCAG
CAAGAATTGGAGATTATNCTGATTTCTATGCATCAAAAGAACATGCAACCAATGTTGGTN
TTATGTTTAGAG----------TTAATCCATGTAGATTATTGGATTTTGAATTGGAGATG
GGCGCTTTGATTGGAGGAGAATCAACCAAATTGGGTGAACCAATTTCAATTGAGTCTGCA
AAAGATCATATCTTTGGTTTGGTACTTTTGAATGATTGGAGTGCAAGAGATATCCAAAAA
TGGGAATACGTTCCATTGGGTCCATTCTTGGCAAAGAATTTCGGCTCAACTATATCACCA
TGGGTTGTTACAATGGAGGCATTGCAACCATTTGCTACAAAACCACCAACTCAAGATCCA
CAACCAATGAAATATCTTCAAGAACAAGGTAATACTACCTTTGATATTGAATTATCAGTT
AGTATTAAATCTCCAAAAATGTCAAAACCTCATAAGGTTTCAACTTCAAATTTGAAATAC
ATGTATTGGACACTCACTCAACAATTGGCTCATCATACTGTAAATGGTTGTAATATGAAT
GCTGGTGATTTACTTGGTACTGGTACAATTAGTGGTCCAACTGAAGATAGCTATGGTTCA
ATGTTGGAACTCTCTTGAAAAGGTAGTAAAGTGGTCTCATTAGGTACTGAAACAAATGAA
GAAAGAAAATTCATTCAAGATGGTGATTCTGTTATCTTATCTGGTCTTTGTAAAGGTAAT
GGTTATCAAATTGGTTTTGGTAATTGTGAAGGTACAATTTTACCAGCTGATAAACGTCAA
TAAAAAAAAATTAATTAAATAAATAAATAAATAAAGAAAACTATTTTTAAAT