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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHF213
Library VH
(Link to library)
Clone ID VHF213
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VHF213P
(Link to Original site)
Representative DNA sequence 
>VHF213 (VHF213Q) /CSM/VH/VHF2-A/VHF213Q.Seq.d/
CTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTCGTGC
CATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATCACGG
TAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAGTTTC
TGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCATTAA
ATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTGGTTG
TACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCAGTTC
AGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCGTCGA
AGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCAAACC
AGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTGAAGA
AGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACACTGA
AGATXXXXXXXXXXCCAGAAGGTAACGGTGCCAATNTCATCGTCAATGTCACCAAAGGTG
TTCAATATTTGAATGAAATTAAAGANTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAG
AAGGTGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCN
TCCACACTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTAN
TATATGCTGATGAATTAACTGCNTCCCCAANTATCTTAGAACCAATCTACTTAGTAGAAA
TTACTGCCCCAGAAAACGCTATTGGTGGTATTTANTCTGTACTCAATCGTCGTAGAGGTA
TTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTATCTGTCAAAGCTCATTTAC
CAGTATTAGAATCNTTCGGTTTCACCGATGATTTACGTTCCCACACTGCCGGTCAAGCTT
TCCCACAATGTGTNTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAG
CCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATC
TTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTNAGTTTTCAA
sequence update 2002.10.25
Translated Amino Acid sequence 
SSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKVS
GDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFSS
EVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTEE
AYLSFRRAIESVNVIVGNTED---

---PEGNGANXIVNVTKGVQYLNEIKXSFVGAFQWATKEGVVCDENMRGIRFNLYDVTXH
TDAIHRGGGQIIPTARRVXYADELTASPXILEPIYLVEITAPENAIGGIXSVLNRRRGIV
IGEERRIGSPLLSVKAHLPVLESFGFTDDLRSHTAGQAFPQCVFDHWASIGVVNKDKKAT
EVALATRKRKGLAPEIPDLDKFHEKL*ti*valxfs
Translated Amino Acid sequence (All Frames) 
Frame A:
leckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*sf
w*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fqf
rsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*r
slslfp*sy*irqchcw*h*r---

---PEGNGANXIVNVTKGVQYLNEIKXSFVGAFQWATKEGVVCDENMRGIRFNLYDVTXH
TDAIHRGGGQIIPTARRVXYADELTASPXILEPIYLVEITAPENAIGGIXSVLNRRRGIV
IGEERRIGSPLLSVKAHLPVLESFGFTDDLRSHTAGQAFPQCVFDHWASIGVVNKDKKAT
EVALATRKRKGLAPEIPDLDKFHEKL*ti*valxfs

Frame B:
SSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKVS
GDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFSS
EVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTEE
AYLSFRRAIESVNVIVGNTED---

---qkvtvpxsssmspkvfni*mklkxlslvlsngppkkvlfvmkt*evsvsifmmspst
lmpstevvvkssqllvvxymlmn*lxpqxs*nqst**kllpqktllvvfxlysivvevll
lvknvvsvphyylskliyqy*nxsvspmiyvptlpvklshnvxsiiglqlvlstkirkpl
ksl*pperekv*pqksqiltnstknskqfkshxsfq

Frame C:
rvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpikfl
vi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlisvq
k*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tlkk
lislsvellnpsmsllvtlk---

---rr*rcqxhrqchqrcsife*n*rxfrwcfpmghqrrccl**kherypfqsl*chxph
*chpqrwwsnhpncsscxic**incxpxylrtnllsrnycprkrywwylxctqss*rycy
w*rtsyrfpiiicqssftsirixrfhr*ftfphcrssfptmcxrslgfnwccqqr*esh*
srfshqkekrfsprnprs*qiprktlnnlsrtxvf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF213 (VHF213Q) /CSM/VH/VHF2-A/VHF213Q.Seq.d/                       2335   0.0
VHE713 (VHE713Q) /CSM/VH/VHE7-A/VHE713Q.Seq.d/                       1209   0.0
VHF758 (VHF758Q) /CSM/VH/VHF7-C/VHF758Q.Seq.d/                       1201   0.0
VHF757 (VHF757Q) /CSM/VH/VHF7-C/VHF757Q.Seq.d/                       1201   0.0
VHF609 (VHF609Q) /CSM/VH/VHF6-A/VHF609Q.Seq.d/                       1201   0.0
VHF604 (VHF604Q) /CSM/VH/VHF6-A/VHF604Q.Seq.d/                       1201   0.0
VHF435 (VHF435Q) /CSM/VH/VHF4-B/VHF435Q.Seq.d/                       1201   0.0
VHF160 (VHF160Q) /CSM/VH/VHF1-C/VHF160Q.Seq.d/                       1201   0.0
VHE857 (VHE857Q) /CSM/VH/VHE8-C/VHE857Q.Seq.d/                       1197   0.0
VHF772 (VHF772Q) /CSM/VH/VHF7-C/VHF772Q.Seq.d/                       1195   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ439636) Dictyostelium discoideum cDNA clone:ddv41i04, 3' ...  1209   0.0    1
(BJ439334) Dictyostelium discoideum cDNA clone:ddv40j03, 3' ...  1209   0.0    1
(BJ440065) Dictyostelium discoideum cDNA clone:ddv42d15, 3' ...  1201   0.0    1
(BJ440053) Dictyostelium discoideum cDNA clone:ddv42b15, 3' ...  1201   0.0    1
(BJ439921) Dictyostelium discoideum cDNA clone:ddv42g02, 3' ...  1201   0.0    1
(BJ439893) Dictyostelium discoideum cDNA clone:ddv42a04, 3' ...  1201   0.0    1
(BJ439779) Dictyostelium discoideum cDNA clone:ddv41g15, 3' ...  1201   0.0    1
(BJ439697) Dictyostelium discoideum cDNA clone:ddv41f10, 3' ...  1201   0.0    1
(BJ439450) Dictyostelium discoideum cDNA clone:ddv40b16, 3' ...  1197   0.0    1
(BJ439816) Dictyostelium discoideum cDNA clone:ddv41o16, 3' ...  1195   0.0    1
dna update 2008.10.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM270011_29(AM270011|pid:none) Aspergillus niger contig An02c016...   264   6e-69
AF248644_1(AF248644|pid:none) Filobasidiella neoformans var. neo...   259   1e-67
(Q6FYA7) RecName: Full=Elongation factor 2;          Short=EF-2;...   258   3e-67
AF083031_87(AF083031|pid:none) Guillardia theta nucleomorph chro...   258   4e-67
AY026074_1(AY026074|pid:none) Monosiga brevicollis elongation fa...   257   7e-67
(Q874B9) RecName: Full=Elongation factor 2;          Short=EF-2;...   257   7e-67
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   256   2e-66
DQ357217_1(DQ357217|pid:none) Leishmania braziliensis elongation...   254   3e-66
AM494972_26(AM494972|pid:none) Leishmania braziliensis chromosom...   254   3e-66
CT005272_19(CT005272|pid:none) Leishmania major strain Friedlin,...   254   5e-66
protein update 2009. 7. 2
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.31  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 40.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHF213 is cyt
5' end seq. ID VHF213F
5' end seq. 
>VHF213F.Seq
CTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTCGTGC
CATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATCACGG
TAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAGTTTC
TGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCATTAA
ATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTGGTTG
TACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCAGTTC
AGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCGTCGA
AGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCAAACC
AGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTGAAGA
AGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACACTGA
AGATNNNNNNNNNN
Length of 5' end seq. 614
3' end seq. ID VHF213Z
3' end seq. 
>VHF213Z.Seq
NNNNNNNNNNCCAGAAGGTAACGGTGCCAATNTCATCGTCAATGTCACCAAAGGTGTTCA
ATATTTGAATGAAATTAAAGANTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGG
TGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCNTCCA
CACTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTANTATA
TGCTGATGAATTAACTGCNTCCCCAANTATCTTAGAACCAATCTACTTAGTAGAAATTAC
TGCCCCAGAAAACGCTATTGGTGGTATTTANTCTGTACTCAATCGTCGTAGAGGTATTGT
TATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTATCTGTCAAAGCTCATTTACCAGT
ATTAGAATCNTTCGGTTTCACCGATGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCC
ACAATGTGTNTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCAC
TGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGA
CAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTNAGTTTTCAA
Length of 3' end seq. 650
Connected seq. ID VHF213P
Connected seq. 
>VHF213P.Seq
CTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTCGTGC
CATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATCACGG
TAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAGTTTC
TGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCATTAA
ATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTGGTTG
TACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCAGTTC
AGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCGTCGA
AGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCAAACC
AGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTGAAGA
AGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACACTGA
AGAT----------CCAGAAGGTAACGGTGCCAATNTCATCGTCAATGTCACCAAAGGTG
TTCAATATTTGAATGAAATTAAAGANTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAG
AAGGTGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCN
TCCACACTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTAN
TATATGCTGATGAATTAACTGCNTCCCCAANTATCTTAGAACCAATCTACTTAGTAGAAA
TTACTGCCCCAGAAAACGCTATTGGTGGTATTTANTCTGTACTCAATCGTCGTAGAGGTA
TTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTATCTGTCAAAGCTCATTTAC
CAGTATTAGAATCNTTCGGTTTCACCGATGATTTACGTTCCCACACTGCCGGTCAAGCTT
TCCCACAATGTGTNTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAG
CCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATC
TTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTNAGTTTTCAA
Length of connected seq. 1244
Full length Seq ID -
Full length Seq. -
Length of full length seq. -