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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHF201
Library VH
(Link to library)
Clone ID VHF201
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15717-1
Original site URL
Representative seq. ID VHF201P
(Link to Original site)
Representative DNA sequence 
>VHF201 (VHF201Q) /CSM/VH/VHF2-A/VHF201Q.Seq.d/
ACAACTTCAAAAATAATATTTTTTTTTATATTATTATATAATTAAAAAAAAATAATTATA
AAATGAACTCACTCGTTAAGGGTATTTCCAAAGTTAGATCAACTAGATCTTTTTCAACAG
TATCAATGAGTCCTCTTGAACCAAACAAGAAACTCAACTACGAAGGTATTGATGCTAAAT
TAAAACAATTCAGACTCCATCACAACAAACCACTCACCCTTGCTGAAAAGATCATTTATG
GTCATTTAGAAGATCCATCAACTAAAGTTGAAAGAGGTATCACTTATCTTAAATTACATC
CAGATCGTGTTGCTATGCAAGATGCCACCGCACAAATGGCTGTATTACAATTTATGTCAG
CTGGTTTACCAGAAACTGCAGTACCAACAACAATTCATTGTGATCATTTAATTGAAGCAT
ATAAAGGTGGTGAAAAAGATTTAGAAGTTGCAAAAGATATCAATAAAGAAGTTTACGATT
TCCTTTCAACCAGTGCTAAGAAATTTGGTATGGGTTTCTGGAAGCCAGGTTCAGGTATTA
TCCATCAAATCGTTTTANAAAATTATGCATTCCCAGGTGGTTTAATGATTGGTACTGATA
GTCATACACCAAATGCTGGTGXXXXXXXXXXACCACCATCACCAAATGGTCAAAACATTA
ACGTTATCGTTGATTCAGAAAGCAGCAGATTACAATTACTCCAACCATTTGCCCCATGGG
ATAAGAAAGATTTAGTCGATATGCAAGTTCTCATCAAGGTTCAAGGTAAATGTACCACCG
ATCACATCTCTATGGCTGGTCCATGGTTGAAATACAGAGGTCATTTGGATAACATTTCCA
ACAATATGTTAATTGGTGCTATCAANTCTGAAAACGGTAAAGCCAACGCTGTTCTCAATC
AATTCACTGGTGAAATCGGTCCAGTCCCAACCGTCGATCGTGACTACAAGAAACGTGGTG
TCAATTGGATCGTCGTTGGTGATGAAAACTATGGTGAAGGTTCATCTCGTGAACATGCTG
CCCTCGAACCACGTCATNTCGGTGGTAAAGCTATCCTCGTTAAATCATTTGCTCGTATCC
ACGAAACCAATNTCAAGAAACAAGGTATCTTACCATTAACCTTTGCTAACCCATCTGATT
ACGATAAAATNTCTGGTGACGATCGTATTTCAATCATTGGTCTCAAAGATCTTGCCCCAG
GTAAACAACTCACTNTCATTGTTAAATCTGATAAACAAGGTTNTGAATTTGAAATTAAAG
CTAATCACACCATGAATGCTGGTCAAATCGAAGGTTCAAAGCTGGTTCCGCTTTAAATTA
TATTAAATCAGAAAAAGCCAAAAAAAATT
sequence update 2002.10.25
Translated Amino Acid sequence 
nfknniffyiii*LKKNNYKMNSLVKGISKVRSTRSFSTVSMSPLEPNKKLNYEGIDAKL
KQFRLHHNKPLTLAEKIIYGHLEDPSTKVERGITYLKLHPDRVAMQDATAQMAVLQFMSA
GLPETAVPTTIHCDHLIEAYKGGEKDLEVAKDINKEVYDFLSTSAKKFGMGFWKPGSGII
HQIVLXNYAFPGGLMIGTDSHTPNAG---

---PPSPNGQNINVIVDSESSRLQLLQPFAPWDKKDLVDMQVLIKVQGKCTTDHISMAGP
WLKYRGHLDNISNNMLIGAIXSENGKANAVLNQFTGEIGPVPTVDRDYKKRGVNWIVVGD
ENYGEGSSREHAALEPRHXGGKAILVKSFARIHETNXKKQGILPLTFANPSDYDKXSGDD
RISIIGLKDLAPGKQLTXIVKSDKQGXEFEIKANHTMNAGQIEGSKLVPL*iilnqkkpk
ki
Translated Amino Acid sequence (All Frames) 
Frame A:
ttskiifffillyn*kkiiik*thslrvfpkldqldlfqqyq*vllnqtrnsttkvlmln
*nnsdsittnhspllkrsfmvi*kihqlklkevslilnyiqivllckmpphkwlyynlcq
lvyqklqyqqqfivii*lkhikvvkki*klqkisikkftisfqpvlrnlvwvsgsqvqvl
siksfxkimhsqvv**lvlivihqmlv---

---ttitkwskh*ryr*frkqqititpticpmg*erfsryasshqgsr*myhrshlygws
mveiqrsfg*hfqqyvnwcyqx*kr*sqrcsqsihw*nrsspnrrs*lqetwcqldrrw*
*klw*rfis*tccprttsxrw*sypr*iicsyprnqxqetryltinlc*pi*lr*nxw*r
syfnhwsqrscpr*tthxhc*i**trx*i*n*s*shhecwsnrrfkagsalnyiksekak
kn

Frame B:
qlqk*yfflyyyiikkk*l*neltr*gyfqs*in*iffnsiness*tkqetqlrry*c*i
ktiqtpsqqtthpc*kdhlwsfrrsin*s*kryhls*itsrsccyarchrtngcitiyvs
wftrncstnnnsl*sfn*si*rw*krfrsckryq*rslrfpfnqc*eiwygflearfryy
psnrfxklciprwfndwy**sytkcw---

---PPSPNGQNINVIVDSESSRLQLLQPFAPWDKKDLVDMQVLIKVQGKCTTDHISMAGP
WLKYRGHLDNISNNMLIGAIXSENGKANAVLNQFTGEIGPVPTVDRDYKKRGVNWIVVGD
ENYGEGSSREHAALEPRHXGGKAILVKSFARIHETNXKKQGILPLTFANPSDYDKXSGDD
RISIIGLKDLAPGKQLTXIVKSDKQGXEFEIKANHTMNAGQIEGSKLVPL*iilnqkkpk
ki

Frame C:
nfknniffyiii*LKKNNYKMNSLVKGISKVRSTRSFSTVSMSPLEPNKKLNYEGIDAKL
KQFRLHHNKPLTLAEKIIYGHLEDPSTKVERGITYLKLHPDRVAMQDATAQMAVLQFMSA
GLPETAVPTTIHCDHLIEAYKGGEKDLEVAKDINKEVYDFLSTSAKKFGMGFWKPGSGII
HQIVLXNYAFPGGLMIGTDSHTPNAG---

---hhhqmvktltlsliqkaadynysnhlphgirki*sickfssrfkvnvppitslwlvh
g*nteviwitfptic*lvlsxlktvkptlfsinslvksvqsqpsivttrnvvsigsslvm
ktmvkvhlvnmlpsnhvxsvvklsslnhllvstkpxsrnkvsyh*pllthlitikxlvti
vfqslvskilpqvnnslsllnlinkvxnlklklitp*mlvkskvqswfrfkly*irksqk
k
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF201 (VHF201Q) /CSM/VH/VHF2-A/VHF201Q.Seq.d/                       2450   0.0
VHJ504 (VHJ504Q) /CSM/VH/VHJ5-A/VHJ504Q.Seq.d/                       1352   0.0
VHJ814 (VHJ814Q) /CSM/VH/VHJ8-A/VHJ814Q.Seq.d/                       1338   0.0
VHJ867 (VHJ867Q) /CSM/VH/VHJ8-C/VHJ867Q.Seq.d/                       1328   0.0
VHO133 (VHO133Q) /CSM/VH/VHO1-B/VHO133Q.Seq.d/                       1322   0.0
VHC367 (VHC367Q) /CSM/VH/VHC3-C/VHC367Q.Seq.d/                       1310   0.0
VHD386 (VHD386Q) /CSM/VH/VHD3-D/VHD386Q.Seq.d/                       1271   0.0
CHQ607 (CHQ607Q) /CSM/CH/CHQ6-A/CHQ607Q.Seq.d/                       1267   0.0
VHN217 (VHN217Q) /CSM/VH/VHN2-A/VHN217Q.Seq.d/                       1217   0.0
SLA476 (SLA476Q) /CSM/SL/SLA4-D/SLA476Q.Seq.d/                       1132   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ439599) Dictyostelium discoideum cDNA clone:ddv41a02, 3' ...  1368   0.0    1
(BJ442499) Dictyostelium discoideum cDNA clone:ddv50g01, 3' ...  1352   0.0    1
(BJ442525) Dictyostelium discoideum cDNA clone:ddv50l04, 3' ...  1259   0.0    2
(BJ437847) Dictyostelium discoideum cDNA clone:ddv35f17, 3' ...  1247   0.0    2
(BJ445359) Dictyostelium discoideum cDNA clone:ddv59a09, 3' ...  1233   0.0    2
(BJ438430) Dictyostelium discoideum cDNA clone:ddv37l21, 3' ...  1229   0.0    2
(BJ385501) Dictyostelium discoideum cDNA clone:ddc55m02, 3' ...  1193   0.0    2
(BJ442653) Dictyostelium discoideum cDNA clone:ddv50f18, 3' ...  1166   0.0    2
(C23825) Dictyostelium discoideum gamete cDNA, clone FCL-AC04.   1120   0.0    2
(BJ421181) Dictyostelium discoideum cDNA clone:ddv41a02, 5' ...  1120   0.0    3
dna update 2008.10.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AB105050_1(AB105050|pid:none) Antheraea yamamai mRNA for hypothe...   297   7e-79
Z49931_1(Z49931|pid:none) B.taurus mRNA for mitochondrial aconit...   293   1e-77
DQ468384_1(DQ468384|pid:none) Bos taurus BTA05 5597 genomic sequ...   293   1e-77
BC102642_1(BC102642|pid:none) Bos taurus aconitase 2, mitochondr...   293   1e-77
(P20004) RecName: Full=Aconitate hydratase, mitochondrial;      ...   293   1e-77
(Q99KI0) RecName: Full=Aconitate hydratase, mitochondrial;      ...   292   2e-77
BC058049_1(BC058049|pid:none) Danio rerio aconitase 2, mitochond...   292   2e-77
AK144207_1(AK144207|pid:none) Mus musculus kidney CCL-142 RAG cD...   292   2e-77
(P49609) RecName: Full=Aconitate hydratase, mitochondrial;      ...   291   4e-77
S44831(S44831)F54H12.1 protein - Caenorhabditis elegans               290   5e-77
protein update 2009. 7. 2
PSORT 

 psg: 0.75  gvh: 0.44  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.47  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 84.0 %: mitochondrial
	  8.0 %: cytoplasmic
	  4.0 %: plasma membrane
	  4.0 %: nuclear

>> prediction for VHF201 is mit
5' end seq. ID VHF201F
5' end seq. 
>VHF201F.Seq
ACAACTTCAAAAATAATATTTTTTTTTATATTATTATATAATTAAAAAAAAATAATTATA
AAATGAACTCACTCGTTAAGGGTATTTCCAAAGTTAGATCAACTAGATCTTTTTCAACAG
TATCAATGAGTCCTCTTGAACCAAACAAGAAACTCAACTACGAAGGTATTGATGCTAAAT
TAAAACAATTCAGACTCCATCACAACAAACCACTCACCCTTGCTGAAAAGATCATTTATG
GTCATTTAGAAGATCCATCAACTAAAGTTGAAAGAGGTATCACTTATCTTAAATTACATC
CAGATCGTGTTGCTATGCAAGATGCCACCGCACAAATGGCTGTATTACAATTTATGTCAG
CTGGTTTACCAGAAACTGCAGTACCAACAACAATTCATTGTGATCATTTAATTGAAGCAT
ATAAAGGTGGTGAAAAAGATTTAGAAGTTGCAAAAGATATCAATAAAGAAGTTTACGATT
TCCTTTCAACCAGTGCTAAGAAATTTGGTATGGGTTTCTGGAAGCCAGGTTCAGGTATTA
TCCATCAAATCGTTTTANAAAATTATGCATTCCCAGGTGGTTTAATGATTGGTACTGATA
GTCATACACCAAATGCTGGTGNNNNNNNNNN
Length of 5' end seq. 631
3' end seq. ID VHF201Z
3' end seq. 
>VHF201Z.Seq
NNNNNNNNNNACCACCATCACCAAATGGTCAAAACATTAACGTTATCGTTGATTCAGAAA
GCAGCAGATTACAATTACTCCAACCATTTGCCCCATGGGATAAGAAAGATTTAGTCGATA
TGCAAGTTCTCATCAAGGTTCAAGGTAAATGTACCACCGATCACATCTCTATGGCTGGTC
CATGGTTGAAATACAGAGGTCATTTGGATAACATTTCCAACAATATGTTAATTGGTGCTA
TCAANTCTGAAAACGGTAAAGCCAACGCTGTTCTCAATCAATTCACTGGTGAAATCGGTC
CAGTCCCAACCGTCGATCGTGACTACAAGAAACGTGGTGTCAATTGGATCGTCGTTGGTG
ATGAAAACTATGGTGAAGGTTCATCTCGTGAACATGCTGCCCTCGAACCACGTCATNTCG
GTGGTAAAGCTATCCTCGTTAAATCATTTGCTCGTATCCACGAAACCAATNTCAAGAAAC
AAGGTATCTTACCATTAACCTTTGCTAACCCATCTGATTACGATAAAATNTCTGGTGACG
ATCGTATTTCAATCATTGGTCTCAAAGATCTTGCCCCAGGTAAACAACTCACTNTCATTG
TTAAATCTGATAAACAAGGTTNTGAATTTGAAATTAAAGCTAATCACACCATGAATGCTG
GTCAAATCGAAGGTTCAAAGCTGGTTCCGCTTTAAATTATATTAAATCAGAAAAAGCCAA
AAAAAATT
Length of 3' end seq. 728
Connected seq. ID VHF201P
Connected seq. 
>VHF201P.Seq
ACAACTTCAAAAATAATATTTTTTTTTATATTATTATATAATTAAAAAAAAATAATTATA
AAATGAACTCACTCGTTAAGGGTATTTCCAAAGTTAGATCAACTAGATCTTTTTCAACAG
TATCAATGAGTCCTCTTGAACCAAACAAGAAACTCAACTACGAAGGTATTGATGCTAAAT
TAAAACAATTCAGACTCCATCACAACAAACCACTCACCCTTGCTGAAAAGATCATTTATG
GTCATTTAGAAGATCCATCAACTAAAGTTGAAAGAGGTATCACTTATCTTAAATTACATC
CAGATCGTGTTGCTATGCAAGATGCCACCGCACAAATGGCTGTATTACAATTTATGTCAG
CTGGTTTACCAGAAACTGCAGTACCAACAACAATTCATTGTGATCATTTAATTGAAGCAT
ATAAAGGTGGTGAAAAAGATTTAGAAGTTGCAAAAGATATCAATAAAGAAGTTTACGATT
TCCTTTCAACCAGTGCTAAGAAATTTGGTATGGGTTTCTGGAAGCCAGGTTCAGGTATTA
TCCATCAAATCGTTTTANAAAATTATGCATTCCCAGGTGGTTTAATGATTGGTACTGATA
GTCATACACCAAATGCTGGTG----------ACCACCATCACCAAATGGTCAAAACATTA
ACGTTATCGTTGATTCAGAAAGCAGCAGATTACAATTACTCCAACCATTTGCCCCATGGG
ATAAGAAAGATTTAGTCGATATGCAAGTTCTCATCAAGGTTCAAGGTAAATGTACCACCG
ATCACATCTCTATGGCTGGTCCATGGTTGAAATACAGAGGTCATTTGGATAACATTTCCA
ACAATATGTTAATTGGTGCTATCAANTCTGAAAACGGTAAAGCCAACGCTGTTCTCAATC
AATTCACTGGTGAAATCGGTCCAGTCCCAACCGTCGATCGTGACTACAAGAAACGTGGTG
TCAATTGGATCGTCGTTGGTGATGAAAACTATGGTGAAGGTTCATCTCGTGAACATGCTG
CCCTCGAACCACGTCATNTCGGTGGTAAAGCTATCCTCGTTAAATCATTTGCTCGTATCC
ACGAAACCAATNTCAAGAAACAAGGTATCTTACCATTAACCTTTGCTAACCCATCTGATT
ACGATAAAATNTCTGGTGACGATCGTATTTCAATCATTGGTCTCAAAGATCTTGCCCCAG
GTAAACAACTCACTNTCATTGTTAAATCTGATAAACAAGGTTNTGAATTTGAAATTAAAG
CTAATCACACCATGAATGCTGGTCAAATCGAAGGTTCAAAGCTGGTTCCGCTTTAAATTA
TATTAAATCAGAAAAAGCCAAAAAAAATT
Length of connected seq. 1339
Full length Seq ID -
Full length Seq. -
Length of full length seq. -