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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHF187
Library VH
(Link to library)
Clone ID VHF187
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16363-1
Original site URL
Representative seq. ID VHF187P
(Link to Original site)
Representative DNA sequence 
>VHF187 (VHF187Q) /CSM/VH/VHF1-D/VHF187Q.Seq.d/
GAATGAAAAGATCAATAGTATTTATTTATTCATTGGTAATTTTATTATTATCAGTTGGGT
TTATTGATGCATTCAAAATATCAATAGAAAATCATATAAAATTATCAGATGATTCATCTT
ATCAAATTGGTACAGGTATTTATGATATTACCGGACCAGGTGCAGAGACTAATATGATGG
GCTATGCAATGCCAGGCCAAATTACTGGAGGAATTCATTTTAGACAACGTGCTAGAGCAT
TTGTATTTATAGATTCAGAAGGTAATAGAGCAGTATATGTTAGTACTGATTCATGTATGA
TTTTCCAAGAAGTTAAAATTCAAGTTATTCAAGATTTACAAGAAATTTTTGGACCAACTT
TATATACACATGATAATGTATTATTAAGCGGTACACATACTCATTCAGGTCCTGCTGGTT
TTTCAGAGTATGCATTATATGGTATTACAGCATTAGGTTTCTATAAAAAGAATTTCGATA
CAATTTGTGATGGTATTGTTCAAGCAATTGTTAAAGCTCATAAATCGGTTCAACCTGCAX
XXXXXXXXXTGGTAGAAGATTAAGAAATACNGTTAGAGAAATCATTGGTCAATCAATTGA
AAATCCAATTGTTTTAATTGCTGGTTTAGCAAATACTTATTCAGGTTATATTGCAACATT
TGAAGAGTTTCAAGTTCAAAGATATGAAGGTGCATCAACCGTTTTTGGACTTCATACTTT
AGGAGCATATCAACAAGAATTTGCAAATCTTGCACAATCAATTGTAGATGGTAGTCAAGC
TGATCCAGGAACATTCCCAAGAAATATGTCTGGTCATACACCATTCTTTTTACCACCAGT
CATTGTTGATGTAGCTCCAAAATTCGACGATTTCGGTGATATCTATACTGATGTCTCTAC
AACTACACCATATTCAATCAATCAAACTGTTACCGTCATTTTCTATGGTGCCAATCTTAG
AAATAATTTCATGACTGAATCTTCTTTCNTCACTGTTGATCAATTACAATCTAATGGTCA
ATGGACTACAATTTTAAATGATGGTGATTGGGATACAAAATTATATTGGAAAATGCATGA
TTTAGGTTTTTCACTCATCACTGTTGATTGGACAATCTCTCCAATCACTCAACCAGGTAC
TTATAGAATAACTCATAGTGGTTAGGCTAAGAAAAATCCATTCTCTNATAATTTAACTTT
TTATCAAGGTATTTCAAGTAATTTTAANGTTCAATAAAAATTAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
MKRSIVFIYSLVILLLSVGFIDAFKISIENHIKLSDDSSYQIGTGIYDITGPGAETNMMG
YAMPGQITGGIHFRQRARAFVFIDSEGNRAVYVSTDSCMIFQEVKIQVIQDLQEIFGPTL
YTHDNVLLSGTHTHSGPAGFSEYALYGITALGFYKKNFDTICDGIVQAIVKAHKSVQPA-
--

---GRRLRNTVREIIGQSIENPIVLIAGLANTYSGYIATFEEFQVQRYEGASTVFGLHTL
GAYQQEFANLAQSIVDGSQADPGTFPRNMSGHTPFFLPPVIVDVAPKFDDFGDIYTDVST
TTPYSINQTVTVIFYGANLRNNFMTESSFXTVDQLQSNGQWTTILNDGDWDTKLYWKMHD
LGFSLITVDWTISPITQPGTYRITHSG*akknpfsxnltfyqgissnfxvq*klk
Translated Amino Acid sequence (All Frames) 
Frame A:
e*kdq*ylfihw*fyyyqlgllmhskyq*kii*nyqmihliklvqvfmilpdqvqrli*w
amqcqaklleefildnvlehlyl*iqkvieqymlvlihv*fskklkfklfkiykkfldql
yihmimyy*avhiliqvllvfqsmhymvlqh*vsikrisiqfvmvlfkqllklinrfnl-
--

---w*kikkyx*rnhwsin*ksncfncwfskylfrlycni*rvssski*rcinrfwtsyf
rsistricksctincrw*ss*srnipkkyvwsytilfttshc*csskirrfr*yly*cly
nytifnqsncyrhflwcqs*k*fhd*iffxhc*siti*wsmdynfk*w*lgykiilena*
frffthhc*ldnlsnhstryl*nns*wlg*eksilx*fnflsryfk*f*xsikik

Frame B:
nekinsiylfignfiiiswvy*ciqninrksykiir*filsnwyryl*yyrtrcrd*ydg
lcnarpnywrnsf*ttc*siciyrfrr**ssic*y*fmydfprs*nssysrftrnfwtnf
iyt**ciikrytysfrscwffrvciiwyysirfl*kefrynl*wycssnc*ss*igstc-
--

---GRRLRNTVREIIGQSIENPIVLIAGLANTYSGYIATFEEFQVQRYEGASTVFGLHTL
GAYQQEFANLAQSIVDGSQADPGTFPRNMSGHTPFFLPPVIVDVAPKFDDFGDIYTDVST
TTPYSINQTVTVIFYGANLRNNFMTESSFXTVDQLQSNGQWTTILNDGDWDTKLYWKMHD
LGFSLITVDWTISPITQPGTYRITHSG*akknpfsxnltfyqgissnfxvq*klk

Frame C:
MKRSIVFIYSLVILLLSVGFIDAFKISIENHIKLSDDSSYQIGTGIYDITGPGAETNMMG
YAMPGQITGGIHFRQRARAFVFIDSEGNRAVYVSTDSCMIFQEVKIQVIQDLQEIFGPTL
YTHDNVLLSGTHTHSGPAGFSEYALYGITALGFYKKNFDTICDGIVQAIVKAHKSVQPA-
--

---ved*eixlekslvnqlkiqlf*llv*qiliqvilqhlksfkfkdmkvhqpfldfil*
ehinknlqilhnql*mvvkliqehsqeiclvihhsfyhqsllm*lqnstisvisilmslq
lhhiqsikllpsfsmvpileiis*lnllssllinynlmvnglqf*mmvigiqnyigkcmi
*vfhsslligqslqslnqvlie*livvrlrkihslii*lfikvfqvilxfnkn*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHF187 (VHF187Q) /CSM/VH/VHF1-D/VHF187Q.Seq.d/                       2387   0.0
VHI396 (VHI396Q) /CSM/VH/VHI3-D/VHI396Q.Seq.d/                       1342   0.0
VHD412 (VHD412Q) /CSM/VH/VHD4-A/VHD412Q.Seq.d/                       1334   0.0
VFM574 (VFM574Q) /CSM/VF/VFM5-D/VFM574Q.Seq.d/                       1334   0.0
AHB843 (AHB843Q) /CSM/AH/AHB8-B/AHB843Q.Seq.d/                       1332   0.0
VHP543 (VHP543Q) /CSM/VH/VHP5-B/VHP543Q.Seq.d/                       1330   0.0
VHD145 (VHD145Q) /CSM/VH/VHD1-B/VHD145Q.Seq.d/                       1330   0.0
AHM465 (AHM465Q) /CSM/AH/AHM4-C/AHM465Q.Seq.d/                       1330   0.0
SFK788 (SFK788Q) /CSM/SF/SFK7-D/SFK788Q.Seq.d/                       1326   0.0
AHB869 (AHB869Q) /CSM/AH/AHB8-C/AHB869Q.Seq.d/                       1320   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U82513|U82513.1 Dictyostelium discoideum random slug cDNA25 protein (rsc25) mRNA, partial cds.  1342   0.0    1
AB121061|AB121061.1 Dictyostelium discoideum dcd2A mRNA for ceramidase, complete cds.  1342   0.0    1
AM045923|AM045923.1 Schistosoma mansoni lung schistosomulum EST, clone SmlC20e12.q1ka.    48   0.003  2
AM047552|AM047552.1 Schistosoma mansoni lung schistosomulum EST, clone SmlC21g08.q1ka.    48   0.003  2
AM047220|AM047220.1 Schistosoma mansoni lung schistosomulum EST, clone SmlC44f10.q1k.    48   0.004  2
AM047012|AM047012.1 Schistosoma mansoni lung schistosomulum EST, clone SmlC20a09.q1ka.    48   0.004  2
AC180850|AC180850.1 Strongylocentrotus purpuratus clone R3-3067P21, WORKING DRAFT SEQUENCE, 21 unordered pieces.    52   0.042  1
AF057019|AF057019.1 Dictyostelium discoideum interaptin (abpD) gene, complete cds.    34   0.15   5
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    34   0.51   10
AC116330|AC116330.2 Dictyostelium discoideum chromosome 2 map 3191214-3323468 strain AX4, complete sequence.    36   0.53   9
dna update 2007.10.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U82513_1(U82513|pid:none) Dictyostelium discoideum random slug c...   456   e-126
(Q55G11) RecName: Full=Neutral ceramidase B;          Short=N-CD...   345   3e-93
AC022464_28(AC022464|pid:none) Genomic sequence for Arabidopsis ...   156   2e-36
(Q304B9) RecName: Full=Neutral ceramidase;          Short=N-CDas...   155   2e-36
CP000777_2353(CP000777|pid:none) Leptospira biflexa serovar Pato...   154   5e-36
EU200974_1(EU200974|pid:none) Triticum aestivum neutral ceramida...   154   5e-36
AP002819_24(AP002819|pid:none) Oryza sativa Japonica Group genom...   153   1e-35
AK099625_1(AK099625|pid:none) Oryza sativa Japonica Group cDNA c...   153   1e-35
AB016885_17(AB016885|pid:none) Arabidopsis thaliana genomic DNA,...   152   2e-35
BC167651_1(BC167651|pid:none) Xenopus tropicalis hypothetical pr...   151   5e-35
protein update 2009. 7. 2
PSORT 

 psg: 0.96  gvh: 0.83  alm: 0.38  top: 0.30  tms: 0.00  mit: 0.37  mip: 0.02
 nuc: 0.03  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: extracellular, including cell wall
	 12.0 %: vacuolar
	  8.0 %: cytoplasmic
	  8.0 %: endoplasmic reticulum
	  4.0 %: mitochondrial
	  4.0 %: Golgi

>> prediction for VHF187 is exc
5' end seq. ID VHF187F
5' end seq. 
>VHF187F.Seq
GAATGAAAAGATCAATAGTATTTATTTATTCATTGGTAATTTTATTATTATCAGTTGGGT
TTATTGATGCATTCAAAATATCAATAGAAAATCATATAAAATTATCAGATGATTCATCTT
ATCAAATTGGTACAGGTATTTATGATATTACCGGACCAGGTGCAGAGACTAATATGATGG
GCTATGCAATGCCAGGCCAAATTACTGGAGGAATTCATTTTAGACAACGTGCTAGAGCAT
TTGTATTTATAGATTCAGAAGGTAATAGAGCAGTATATGTTAGTACTGATTCATGTATGA
TTTTCCAAGAAGTTAAAATTCAAGTTATTCAAGATTTACAAGAAATTTTTGGACCAACTT
TATATACACATGATAATGTATTATTAAGCGGTACACATACTCATTCAGGTCCTGCTGGTT
TTTCAGAGTATGCATTATATGGTATTACAGCATTAGGTTTCTATAAAAAGAATTTCGATA
CAATTTGTGATGGTATTGTTCAAGCAATTGTTAAAGCTCATAAATCGGTTCAACCTGCAN
NNNNNNNNN
Length of 5' end seq. 549
3' end seq. ID VHF187Z
3' end seq. 
>VHF187Z.Seq
NNNNNNNNNNTGGTAGAAGATTAAGAAATACNGTTAGAGAAATCATTGGTCAATCAATTG
AAAATCCAATTGTTTTAATTGCTGGTTTAGCAAATACTTATTCAGGTTATATTGCAACAT
TTGAAGAGTTTCAAGTTCAAAGATATGAAGGTGCATCAACCGTTTTTGGACTTCATACTT
TAGGAGCATATCAACAAGAATTTGCAAATCTTGCACAATCAATTGTAGATGGTAGTCAAG
CTGATCCAGGAACATTCCCAAGAAATATGTCTGGTCATACACCATTCTTTTTACCACCAG
TCATTGTTGATGTAGCTCCAAAATTCGACGATTTCGGTGATATCTATACTGATGTCTCTA
CAACTACACCATATTCAATCAATCAAACTGTTACCGTCATTTTCTATGGTGCCAATCTTA
GAAATAATTTCATGACTGAATCTTCTTTCNTCACTGTTGATCAATTACAATCTAATGGTC
AATGGACTACAATTTTAAATGATGGTGATTGGGATACAAAATTATATTGGAAAATGCATG
ATTTAGGTTTTTCACTCATCACTGTTGATTGGACAATCTCTCCAATCACTCAACCAGGTA
CTTATAGAATAACTCATAGTGGTTAGGCTAAGAAAAATCCATTCTCTNATAATTTAACTT
TTTATCAAGGTATTTCAAGTAATTTTAANGTTCAATAAAAATTAAAA
Length of 3' end seq. 707
Connected seq. ID VHF187P
Connected seq. 
>VHF187P.Seq
GAATGAAAAGATCAATAGTATTTATTTATTCATTGGTAATTTTATTATTATCAGTTGGGT
TTATTGATGCATTCAAAATATCAATAGAAAATCATATAAAATTATCAGATGATTCATCTT
ATCAAATTGGTACAGGTATTTATGATATTACCGGACCAGGTGCAGAGACTAATATGATGG
GCTATGCAATGCCAGGCCAAATTACTGGAGGAATTCATTTTAGACAACGTGCTAGAGCAT
TTGTATTTATAGATTCAGAAGGTAATAGAGCAGTATATGTTAGTACTGATTCATGTATGA
TTTTCCAAGAAGTTAAAATTCAAGTTATTCAAGATTTACAAGAAATTTTTGGACCAACTT
TATATACACATGATAATGTATTATTAAGCGGTACACATACTCATTCAGGTCCTGCTGGTT
TTTCAGAGTATGCATTATATGGTATTACAGCATTAGGTTTCTATAAAAAGAATTTCGATA
CAATTTGTGATGGTATTGTTCAAGCAATTGTTAAAGCTCATAAATCGGTTCAACCTGCA-
---------TGGTAGAAGATTAAGAAATACNGTTAGAGAAATCATTGGTCAATCAATTGA
AAATCCAATTGTTTTAATTGCTGGTTTAGCAAATACTTATTCAGGTTATATTGCAACATT
TGAAGAGTTTCAAGTTCAAAGATATGAAGGTGCATCAACCGTTTTTGGACTTCATACTTT
AGGAGCATATCAACAAGAATTTGCAAATCTTGCACAATCAATTGTAGATGGTAGTCAAGC
TGATCCAGGAACATTCCCAAGAAATATGTCTGGTCATACACCATTCTTTTTACCACCAGT
CATTGTTGATGTAGCTCCAAAATTCGACGATTTCGGTGATATCTATACTGATGTCTCTAC
AACTACACCATATTCAATCAATCAAACTGTTACCGTCATTTTCTATGGTGCCAATCTTAG
AAATAATTTCATGACTGAATCTTCTTTCNTCACTGTTGATCAATTACAATCTAATGGTCA
ATGGACTACAATTTTAAATGATGGTGATTGGGATACAAAATTATATTGGAAAATGCATGA
TTTAGGTTTTTCACTCATCACTGTTGATTGGACAATCTCTCCAATCACTCAACCAGGTAC
TTATAGAATAACTCATAGTGGTTAGGCTAAGAAAAATCCATTCTCTNATAATTTAACTTT
TTATCAAGGTATTTCAAGTAATTTTAANGTTCAATAAAAATTAAAA
Length of connected seq. 1236
Full length Seq ID -
Full length Seq. -
Length of full length seq. -